BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9ZKJ5 (325 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3AKJ-A nrPDB 678 0.0 5N6Y-C nrPDB 32 0.29 2A79-B nrPDB 32 0.31 2QAG-A nrPDB 30 1.1 5J45-A nrPDB 29 2.2 4YDH-A nrPDB 29 2.3 4UUG-A nrPDB 28 3.8 3FTQ-A nrPDB 28 4.1 3UAI-C nrPDB 28 5.7 1SQ1-A nrPDB 28 6.8 >3AKJ-A nrPDB Length = 325 Score = 678 bits (1750), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/325 (100%), Positives = 325/325 (100%) Query: 1 MPTIDFTFCEINPKKGFGGANGNKISLFYNNELYMVKFPPKPSTHKEMSYTNGCFSEYVA 60 MPTIDFTFCEINPKKGFGGANGNKISLFYNNELYMVKFPPKPSTHKEMSYTNGCFSEYVA Sbjct: 1 MPTIDFTFCEINPKKGFGGANGNKISLFYNNELYMVKFPPKPSTHKEMSYTNGCFSEYVA 60 Query: 61 CHIVNSLGLKVQETLLGTYKNKIVVACKDFTTHQYELVDFLSLKNTMIELEKSGKDTNLN 120 CHIVNSLGLKVQETLLGTYKNKIVVACKDFTTHQYELVDFLSLKNTMIELEKSGKDTNLN Sbjct: 61 CHIVNSLGLKVQETLLGTYKNKIVVACKDFTTHQYELVDFLSLKNTMIELEKSGKDTNLN 120 Query: 121 DVLYAIDNQHFIEPKVLKCFFWDMFVADTLLGNFDRHNGNWGFLRASNSKEYQIAPIFDC 180 DVLYAIDNQHFIEPKVLKCFFWDMFVADTLLGNFDRHNGNWGFLRASNSKEYQIAPIFDC Sbjct: 121 DVLYAIDNQHFIEPKVLKCFFWDMFVADTLLGNFDRHNGNWGFLRASNSKEYQIAPIFDC 180 Query: 181 GSCLYPQADDVVCQKVLSNIDELNARIYNFPQSILKDDNDKKINYYDFLTQTNNKDCLDA 240 GSCLYPQADDVVCQKVLSNIDELNARIYNFPQSILKDDNDKKINYYDFLTQTNNKDCLDA Sbjct: 181 GSCLYPQADDVVCQKVLSNIDELNARIYNFPQSILKDDNDKKINYYDFLTQTNNKDCLDA 240 Query: 241 LLRIYPRIDMNKIHSIIDNTPFMSEIHKEFLHTMLDERKSKIIDVAHTRAIELSLQHKQA 300 LLRIYPRIDMNKIHSIIDNTPFMSEIHKEFLHTMLDERKSKIIDVAHTRAIELSLQHKQA Sbjct: 241 LLRIYPRIDMNKIHSIIDNTPFMSEIHKEFLHTMLDERKSKIIDVAHTRAIELSLQHKQA 300 Query: 301 HSNPYDNADDLDNSNEYTPTPKRRR 325 HSNPYDNADDLDNSNEYTPTPKRRR Sbjct: 301 HSNPYDNADDLDNSNEYTPTPKRRR 325 >5N6Y-C nrPDB Length = 113 Score = 32.3 bits (72), Expect = 0.29, Method: Compositional matrix adjust. Identities = 14/37 (37%), Positives = 24/37 (64%) Query: 261 PFMSEIHKEFLHTMLDERKSKIIDVAHTRAIELSLQH 297 P+ S+++KE + +LD KS+++DV TR+ L H Sbjct: 74 PWASQVNKEEIEFLLDGLKSRLVDVTITRSTNRELNH 110 >2A79-B nrPDB Length = 499 Score = 32.3 bits (72), Expect = 0.31, Method: Compositional matrix adjust. Identities = 21/69 (30%), Positives = 34/69 (49%), Gaps = 10/69 (14%) Query: 186 PQADDVVCQKVLSNIDELN-----ARIYNFPQSILKDDNDKKINYYDFLTQT----NNKD 236 P+AD C++V+ NI L + FP+++L D K++ Y+D L N+ Sbjct: 25 PEADHECCERVVINISGLRFETQLKTLAQFPETLLGDPK-KRMRYFDPLRNEYFFDRNRP 83 Query: 237 CLDALLRIY 245 DA+L Y Sbjct: 84 SFDAILYYY 92 >2QAG-A nrPDB Length = 361 Score = 30.4 bits (67), Expect = 1.1, Method: Compositional matrix adjust. Identities = 22/98 (22%), Positives = 46/98 (46%), Gaps = 7/98 (7%) Query: 125 AIDNQHFIEPKVLKCFFWDMFVADTLLGNFDRHNGNWGFLRASNSKEYQIAPIFDCGSCL 184 ++ +H I+ +V CF++ L + F++A ++K I P+ L Sbjct: 135 GLNRRHIIDNRVHCCFYFISPFGHGL------KPLDVAFMKAIHNK-VNIVPVIAKADTL 187 Query: 185 YPQADDVVCQKVLSNIDELNARIYNFPQSILKDDNDKK 222 + + + +++L I+E N +IY+ P + +D D K Sbjct: 188 TLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFK 225 >5J45-A nrPDB Length = 127 Score = 29.3 bits (64), Expect = 2.2, Method: Compositional matrix adjust. Identities = 11/39 (28%), Positives = 23/39 (58%) Query: 232 TNNKDCLDALLRIYPRIDMNKIHSIIDNTPFMSEIHKEF 270 T K+ DAL R + +D++K+H ++D+ ++ +E Sbjct: 87 TTMKNAADALKRAHQNMDVDKVHDMMDDIAEQQDVAREI 125 >4YDH-A nrPDB Length = 433 Score = 29.3 bits (64), Expect = 2.3, Method: Compositional matrix adjust. Identities = 21/57 (36%), Positives = 28/57 (49%), Gaps = 3/57 (5%) Query: 21 NGNKISLFYNNELYMVKFPPKPSTHKEMSYTN-GCFSEYVACHIVNSLGLK--VQET 74 N K L + E + VK PP K SY + G S VA +++LG K VQE+ Sbjct: 63 NEKKWELICDQERFQVKNPPAAYIQKLKSYVDTGGVSRKVAADWMSNLGFKRRVQES 119 >4UUG-A nrPDB Length = 332 Score = 28.5 bits (62), Expect = 3.8, Method: Compositional matrix adjust. Identities = 11/33 (33%), Positives = 17/33 (51%) Query: 7 TFCEINPKKGFGGANGNKISLFYNNELYMVKFP 39 F E+ +G G G+K YNN +Y++ P Sbjct: 112 AFVEVIVTRGLTGVRGSKPEDLYNNNIYLLVLP 144 >3FTQ-A nrPDB Length = 274 Score = 28.5 bits (62), Expect = 4.1, Method: Compositional matrix adjust. Identities = 21/98 (21%), Positives = 46/98 (46%), Gaps = 7/98 (7%) Query: 125 AIDNQHFIEPKVLKCFFWDMFVADTLLGNFDRHNGNWGFLRASNSKEYQIAPIFDCGSCL 184 ++ +H I+ +V CF++ L + F++A ++K I P+ L Sbjct: 103 GLNRRHIIDNRVHCCFYFISPFGHGL------KPLDVAFMKAIHNK-VNIVPVIAKADTL 155 Query: 185 YPQADDVVCQKVLSNIDELNARIYNFPQSILKDDNDKK 222 + + + +++L I+E + +IY+ P + +D D K Sbjct: 156 TLKERERLKKRILDEIEEHSIKIYHLPDAESDEDEDFK 193 >3UAI-C nrPDB Length = 114 Score = 28.1 bits (61), Expect = 5.7, Method: Compositional matrix adjust. Identities = 11/32 (34%), Positives = 19/32 (59%) Query: 177 IFDCGSCLYPQADDVVCQKVLSNIDELNARIY 208 + + G+ L+P D+VC+ + + I NA IY Sbjct: 27 VLEMGAFLHPCEGDIVCRSINTKIPYFNAPIY 58 >1SQ1-A nrPDB Length = 370 Score = 27.7 bits (60), Expect = 6.8, Method: Compositional matrix adjust. Identities = 23/78 (29%), Positives = 32/78 (41%), Gaps = 19/78 (24%) Query: 253 IHSIIDNTPFMSEIHKEFLHTMLDERKS--------KIIDVAHTRA-----------IEL 293 + IID P + +EFL LD+RK K D A + I + Sbjct: 21 VGCIIDGMPAGVKFDEEFLQNELDKRKGGSKFATPRKESDKAQVLSGVFEGYTTGHPIAI 80 Query: 294 SLQHKQAHSNPYDNADDL 311 + ++ AHS YDN DL Sbjct: 81 VVFNENAHSKDYDNLKDL 98 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3AKJ-A nrPDB 701 0.0 Sequences not found previously or not previously below threshold: 5N6Y-C nrPDB 33 0.14 2A79-B nrPDB 33 0.21 2QAG-A nrPDB 30 1.7 5J45-A nrPDB 29 3.3 4YDH-A nrPDB 28 3.8 4UUG-A nrPDB 28 5.2 5T4O-G nrPDB 28 5.3 1FS0-G nrPDB 28 5.3 1SQ1-A nrPDB 28 6.1 3UAI-C nrPDB 28 6.2 5WVM-A nrPDB 28 6.9 3FTQ-A nrPDB 28 7.5 1VJL-A nrPDB 27 9.7 CONVERGED! >3AKJ-A nrPDB Length = 325 Score = 701 bits (1808), Expect = 0.0, Method: Composition-based stats. Identities = 325/325 (100%), Positives = 325/325 (100%) Query: 1 MPTIDFTFCEINPKKGFGGANGNKISLFYNNELYMVKFPPKPSTHKEMSYTNGCFSEYVA 60 MPTIDFTFCEINPKKGFGGANGNKISLFYNNELYMVKFPPKPSTHKEMSYTNGCFSEYVA Sbjct: 1 MPTIDFTFCEINPKKGFGGANGNKISLFYNNELYMVKFPPKPSTHKEMSYTNGCFSEYVA 60 Query: 61 CHIVNSLGLKVQETLLGTYKNKIVVACKDFTTHQYELVDFLSLKNTMIELEKSGKDTNLN 120 CHIVNSLGLKVQETLLGTYKNKIVVACKDFTTHQYELVDFLSLKNTMIELEKSGKDTNLN Sbjct: 61 CHIVNSLGLKVQETLLGTYKNKIVVACKDFTTHQYELVDFLSLKNTMIELEKSGKDTNLN 120 Query: 121 DVLYAIDNQHFIEPKVLKCFFWDMFVADTLLGNFDRHNGNWGFLRASNSKEYQIAPIFDC 180 DVLYAIDNQHFIEPKVLKCFFWDMFVADTLLGNFDRHNGNWGFLRASNSKEYQIAPIFDC Sbjct: 121 DVLYAIDNQHFIEPKVLKCFFWDMFVADTLLGNFDRHNGNWGFLRASNSKEYQIAPIFDC 180 Query: 181 GSCLYPQADDVVCQKVLSNIDELNARIYNFPQSILKDDNDKKINYYDFLTQTNNKDCLDA 240 GSCLYPQADDVVCQKVLSNIDELNARIYNFPQSILKDDNDKKINYYDFLTQTNNKDCLDA Sbjct: 181 GSCLYPQADDVVCQKVLSNIDELNARIYNFPQSILKDDNDKKINYYDFLTQTNNKDCLDA 240 Query: 241 LLRIYPRIDMNKIHSIIDNTPFMSEIHKEFLHTMLDERKSKIIDVAHTRAIELSLQHKQA 300 LLRIYPRIDMNKIHSIIDNTPFMSEIHKEFLHTMLDERKSKIIDVAHTRAIELSLQHKQA Sbjct: 241 LLRIYPRIDMNKIHSIIDNTPFMSEIHKEFLHTMLDERKSKIIDVAHTRAIELSLQHKQA 300 Query: 301 HSNPYDNADDLDNSNEYTPTPKRRR 325 HSNPYDNADDLDNSNEYTPTPKRRR Sbjct: 301 HSNPYDNADDLDNSNEYTPTPKRRR 325 >5N6Y-C nrPDB Length = 113 Score = 33.5 bits (75), Expect = 0.14, Method: Composition-based stats. Identities = 14/37 (37%), Positives = 24/37 (64%) Query: 261 PFMSEIHKEFLHTMLDERKSKIIDVAHTRAIELSLQH 297 P+ S+++KE + +LD KS+++DV TR+ L H Sbjct: 74 PWASQVNKEEIEFLLDGLKSRLVDVTITRSTNRELNH 110 >2A79-B nrPDB Length = 499 Score = 32.7 bits (73), Expect = 0.21, Method: Composition-based stats. Identities = 21/69 (30%), Positives = 34/69 (49%), Gaps = 10/69 (14%) Query: 186 PQADDVVCQKVLSNIDELN-----ARIYNFPQSILKDDNDKKINYYDFLTQT----NNKD 236 P+AD C++V+ NI L + FP+++L D K++ Y+D L N+ Sbjct: 25 PEADHECCERVVINISGLRFETQLKTLAQFPETLLGDPK-KRMRYFDPLRNEYFFDRNRP 83 Query: 237 CLDALLRIY 245 DA+L Y Sbjct: 84 SFDAILYYY 92 >2QAG-A nrPDB Length = 361 Score = 29.6 bits (65), Expect = 1.7, Method: Composition-based stats. Identities = 22/98 (22%), Positives = 46/98 (46%), Gaps = 7/98 (7%) Query: 125 AIDNQHFIEPKVLKCFFWDMFVADTLLGNFDRHNGNWGFLRASNSKEYQIAPIFDCGSCL 184 ++ +H I+ +V CF++ L + F++A ++K I P+ L Sbjct: 135 GLNRRHIIDNRVHCCFYFISPFGHGL------KPLDVAFMKAIHNK-VNIVPVIAKADTL 187 Query: 185 YPQADDVVCQKVLSNIDELNARIYNFPQSILKDDNDKK 222 + + + +++L I+E N +IY+ P + +D D K Sbjct: 188 TLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFK 225 >5J45-A nrPDB Length = 127 Score = 28.8 bits (63), Expect = 3.3, Method: Composition-based stats. Identities = 11/38 (28%), Positives = 23/38 (60%) Query: 232 TNNKDCLDALLRIYPRIDMNKIHSIIDNTPFMSEIHKE 269 T K+ DAL R + +D++K+H ++D+ ++ +E Sbjct: 87 TTMKNAADALKRAHQNMDVDKVHDMMDDIAEQQDVARE 124 >4YDH-A nrPDB Length = 433 Score = 28.5 bits (62), Expect = 3.8, Method: Composition-based stats. Identities = 21/57 (36%), Positives = 28/57 (49%), Gaps = 3/57 (5%) Query: 21 NGNKISLFYNNELYMVKFPPKPSTHKEMSYTN-GCFSEYVACHIVNSLGLK--VQET 74 N K L + E + VK PP K SY + G S VA +++LG K VQE+ Sbjct: 63 NEKKWELICDQERFQVKNPPAAYIQKLKSYVDTGGVSRKVAADWMSNLGFKRRVQES 119 >4UUG-A nrPDB Length = 332 Score = 28.1 bits (61), Expect = 5.2, Method: Composition-based stats. Identities = 11/32 (34%), Positives = 17/32 (53%) Query: 8 FCEINPKKGFGGANGNKISLFYNNELYMVKFP 39 F E+ +G G G+K YNN +Y++ P Sbjct: 113 FVEVIVTRGLTGVRGSKPEDLYNNNIYLLVLP 144 >5T4O-G nrPDB Length = 287 Score = 28.1 bits (61), Expect = 5.3, Method: Composition-based stats. Identities = 13/29 (44%), Positives = 18/29 (62%) Query: 217 DDNDKKINYYDFLTQTNNKDCLDALLRIY 245 DD+D K +D+L + + K LD LLR Y Sbjct: 195 DDDDLKHKSWDYLYEPDPKALLDTLLRRY 223 >1FS0-G nrPDB Length = 230 Score = 28.1 bits (61), Expect = 5.3, Method: Composition-based stats. Identities = 13/29 (44%), Positives = 18/29 (62%) Query: 217 DDNDKKINYYDFLTQTNNKDCLDALLRIY 245 DD+D K +D+L + + K LD LLR Y Sbjct: 177 DDDDLKHKSWDYLYEPDPKALLDTLLRRY 205 >1SQ1-A nrPDB Length = 370 Score = 28.1 bits (61), Expect = 6.1, Method: Composition-based stats. Identities = 23/75 (30%), Positives = 31/75 (41%), Gaps = 19/75 (25%) Query: 256 IIDNTPFMSEIHKEFLHTMLDERKS--------KIIDVAHTRA-----------IELSLQ 296 IID P + +EFL LD+RK K D A + I + + Sbjct: 24 IIDGMPAGVKFDEEFLQNELDKRKGGSKFATPRKESDKAQVLSGVFEGYTTGHPIAIVVF 83 Query: 297 HKQAHSNPYDNADDL 311 ++ AHS YDN DL Sbjct: 84 NENAHSKDYDNLKDL 98 >3UAI-C nrPDB Length = 114 Score = 28.1 bits (61), Expect = 6.2, Method: Composition-based stats. Identities = 11/32 (34%), Positives = 19/32 (59%) Query: 177 IFDCGSCLYPQADDVVCQKVLSNIDELNARIY 208 + + G+ L+P D+VC+ + + I NA IY Sbjct: 27 VLEMGAFLHPCEGDIVCRSINTKIPYFNAPIY 58 >5WVM-A nrPDB Length = 509 Score = 27.7 bits (60), Expect = 6.9, Method: Composition-based stats. Identities = 12/26 (46%), Positives = 16/26 (61%) Query: 157 HNGNWGFLRASNSKEYQIAPIFDCGS 182 H+GNW FLR ++ YQI F+ S Sbjct: 402 HHGNWIFLRNNDQVVYQIMRSFEQNS 427 >3FTQ-A nrPDB Length = 274 Score = 27.7 bits (60), Expect = 7.5, Method: Composition-based stats. Identities = 21/98 (21%), Positives = 46/98 (46%), Gaps = 7/98 (7%) Query: 125 AIDNQHFIEPKVLKCFFWDMFVADTLLGNFDRHNGNWGFLRASNSKEYQIAPIFDCGSCL 184 ++ +H I+ +V CF++ L + F++A ++K I P+ L Sbjct: 103 GLNRRHIIDNRVHCCFYFISPFGHGL------KPLDVAFMKAIHNK-VNIVPVIAKADTL 155 Query: 185 YPQADDVVCQKVLSNIDELNARIYNFPQSILKDDNDKK 222 + + + +++L I+E + +IY+ P + +D D K Sbjct: 156 TLKERERLKKRILDEIEEHSIKIYHLPDAESDEDEDFK 193 >1VJL-A nrPDB Length = 164 Score = 27.3 bits (59), Expect = 9.7, Method: Composition-based stats. Identities = 11/39 (28%), Positives = 25/39 (64%) Query: 247 RIDMNKIHSIIDNTPFMSEIHKEFLHTMLDERKSKIIDV 285 R+D IHS+ DNT + + + ++ +T ++ ++ +ID+ Sbjct: 83 RVDKVIIHSLKDNTFYATLVIRDLTYTDEEDEEAALIDI 121 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.320 0.137 0.418 Lambda K H 0.267 0.0438 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 15,748,953 Number of Sequences: 36641 Number of extensions: 736205 Number of successful extensions: 1693 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 19 Number of HSP's that attempted gapping in prelim test: 1670 Number of HSP's gapped (non-prelim): 41 length of query: 325 length of database: 10,336,785 effective HSP length: 97 effective length of query: 228 effective length of database: 6,782,608 effective search space: 1546434624 effective search space used: 1546434624 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 59 (27.2 bits)