BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9Y821 (239 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5N9J-G nrPDB 497 e-141 5SVA-T nrPDB 54 9e-08 5OQM-h nrPDB 52 2e-07 4UFC-A nrPDB 32 0.27 4MYG-A nrPDB 27 9.3 >5N9J-G nrPDB Length = 239 Score = 497 bits (1279), Expect = e-141, Method: Compositional matrix adjust. Identities = 239/239 (100%), Positives = 239/239 (100%) Query: 1 MEYQRAIDSIEECLNKQLRLSSEKVDQYVLIENWTSLVGHLKTLHSLISNYTNGRELQNE 60 MEYQRAIDSIEECLNKQLRLSSEKVDQYVLIENWTSLVGHLKTLHSLISNYTNGRELQNE Sbjct: 1 MEYQRAIDSIEECLNKQLRLSSEKVDQYVLIENWTSLVGHLKTLHSLISNYTNGRELQNE 60 Query: 61 ISSLLKQDKELDLQIQDCMREMTSIYDTHLPKTVSGRKRQKVNAETLLDYGRKLSKFSSA 120 ISSLLKQDKELDLQIQDCMREMTSIYDTHLPKTVSGRKRQKVNAETLLDYGRKLSKFSSA Sbjct: 61 ISSLLKQDKELDLQIQDCMREMTSIYDTHLPKTVSGRKRQKVNAETLLDYGRKLSKFSSA 120 Query: 121 PPGYNPETGQDAKAPVHYPWPSEDQMRKTLLFQFSTSMVPNLSATASQLFSEQPPKTNEP 180 PPGYNPETGQDAKAPVHYPWPSEDQMRKTLLFQFSTSMVPNLSATASQLFSEQPPKTNEP Sbjct: 121 PPGYNPETGQDAKAPVHYPWPSEDQMRKTLLFQFSTSMVPNLSATASQLFSEQPPKTNEP 180 Query: 181 TETETEIDANKAVEEKTKMNYPASPTFTTQEENKEVESPANKDVFAGFDLFDPEMEEDF 239 TETETEIDANKAVEEKTKMNYPASPTFTTQEENKEVESPANKDVFAGFDLFDPEMEEDF Sbjct: 181 TETETEIDANKAVEEKTKMNYPASPTFTTQEENKEVESPANKDVFAGFDLFDPEMEEDF 239 >5SVA-T nrPDB Length = 284 Score = 53.5 bits (127), Expect = 9e-08, Method: Compositional matrix adjust. Identities = 33/125 (26%), Positives = 64/125 (51%), Gaps = 7/125 (5%) Query: 30 LIENWTSLVGHLKTLHSLISNYTNGRELQNEISSLLKQDKELDLQIQDCMREMTSI---Y 86 LI +L ++K L + Y N +++ + L + +++ + +C E+ ++ Sbjct: 73 LIRTDEALFENVKLLAEYDNIYRNLQKIDKDSEELDSKTRKILEILNECHDELKALPMLE 132 Query: 87 DTHLPKTVSGRKRQKVNAETLLDYGRKLSKFSSAPPGYNPETGQDAKAPVHYPWPSEDQM 146 K ++R K+N+ LLDY KLSKF+ PP ++ + A P ++ WP+ED + Sbjct: 133 QVEFEKNTILQQRSKINSTELLDYATKLSKFTKIPPTFD----KGAVGPNNFIWPAEDAL 188 Query: 147 RKTLL 151 R+ +L Sbjct: 189 RRGML 193 >5OQM-h nrPDB Length = 300 Score = 52.4 bits (124), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 22/55 (40%), Positives = 36/55 (65%), Gaps = 4/55 (7%) Query: 97 RKRQKVNAETLLDYGRKLSKFSSAPPGYNPETGQDAKAPVHYPWPSEDQMRKTLL 151 ++R K+N+ LLDY KLSKF+ PP ++ + A P ++ WP+ED +R+ +L Sbjct: 159 QQRSKINSTELLDYATKLSKFTKIPPTFD----KGAVGPNNFIWPAEDALRRGML 209 >4UFC-A nrPDB Length = 811 Score = 32.0 bits (71), Expect = 0.27, Method: Compositional matrix adjust. Identities = 26/77 (33%), Positives = 31/77 (40%), Gaps = 2/77 (2%) Query: 96 GRKRQKVNAETLLDYGRKLSKFSSAPPGYNPETGQDA-KAPVHYPWPSEDQMRKTLLFQF 154 G A L YGR L SS+ PG P Q H PW S+ + + Sbjct: 321 GNGEDMAMAALLFHYGRYL-LISSSQPGGQPANLQGIWNNSTHAPWDSKYTININTEMNY 379 Query: 155 STSMVPNLSATASQLFS 171 + V NLS T S LFS Sbjct: 380 WPAEVTNLSETHSPLFS 396 >4MYG-A nrPDB Length = 372 Score = 26.6 bits (57), Expect = 9.3, Method: Compositional matrix adjust. Identities = 14/53 (26%), Positives = 23/53 (43%) Query: 167 SQLFSEQPPKTNEPTETETEIDANKAVEEKTKMNYPASPTFTTQEENKEVESP 219 +QLF P+ + E E+D +K + + +P F EE E + P Sbjct: 291 TQLFPRASPQAADLLEKMLELDVDKRLTAAQALTHPFFEPFRDPEEETEAQQP 343 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5N9J-G nrPDB 413 e-116 5SVA-T nrPDB 174 3e-44 5OQM-h nrPDB 166 1e-41 Sequences not found previously or not previously below threshold: 4MYG-A nrPDB 32 0.26 1M7S-A nrPDB 31 0.53 5G5P-A nrPDB 31 0.62 5TEY-A nrPDB 30 0.75 4UFC-A nrPDB 30 0.94 1YR7-A nrPDB 30 1.2 6I7T-G nrPDB 30 1.2 5FWP-E nrPDB 29 1.4 3FII-A nrPDB 28 4.3 5CVY-A nrPDB 28 4.4 6B3O-A nrPDB 28 4.5 2NCA-A nrPDB 27 7.1 CONVERGED! >5N9J-G nrPDB Length = 239 Score = 413 bits (1061), Expect = e-116, Method: Composition-based stats. Identities = 239/239 (100%), Positives = 239/239 (100%) Query: 1 MEYQRAIDSIEECLNKQLRLSSEKVDQYVLIENWTSLVGHLKTLHSLISNYTNGRELQNE 60 MEYQRAIDSIEECLNKQLRLSSEKVDQYVLIENWTSLVGHLKTLHSLISNYTNGRELQNE Sbjct: 1 MEYQRAIDSIEECLNKQLRLSSEKVDQYVLIENWTSLVGHLKTLHSLISNYTNGRELQNE 60 Query: 61 ISSLLKQDKELDLQIQDCMREMTSIYDTHLPKTVSGRKRQKVNAETLLDYGRKLSKFSSA 120 ISSLLKQDKELDLQIQDCMREMTSIYDTHLPKTVSGRKRQKVNAETLLDYGRKLSKFSSA Sbjct: 61 ISSLLKQDKELDLQIQDCMREMTSIYDTHLPKTVSGRKRQKVNAETLLDYGRKLSKFSSA 120 Query: 121 PPGYNPETGQDAKAPVHYPWPSEDQMRKTLLFQFSTSMVPNLSATASQLFSEQPPKTNEP 180 PPGYNPETGQDAKAPVHYPWPSEDQMRKTLLFQFSTSMVPNLSATASQLFSEQPPKTNEP Sbjct: 121 PPGYNPETGQDAKAPVHYPWPSEDQMRKTLLFQFSTSMVPNLSATASQLFSEQPPKTNEP 180 Query: 181 TETETEIDANKAVEEKTKMNYPASPTFTTQEENKEVESPANKDVFAGFDLFDPEMEEDF 239 TETETEIDANKAVEEKTKMNYPASPTFTTQEENKEVESPANKDVFAGFDLFDPEMEEDF Sbjct: 181 TETETEIDANKAVEEKTKMNYPASPTFTTQEENKEVESPANKDVFAGFDLFDPEMEEDF 239 >5SVA-T nrPDB Length = 284 Score = 174 bits (441), Expect = 3e-44, Method: Composition-based stats. Identities = 33/125 (26%), Positives = 64/125 (51%), Gaps = 7/125 (5%) Query: 30 LIENWTSLVGHLKTLHSLISNYTNGRELQNEISSLLKQDKELDLQIQDCMREMTS---IY 86 LI +L ++K L + Y N +++ + L + +++ + +C E+ + + Sbjct: 73 LIRTDEALFENVKLLAEYDNIYRNLQKIDKDSEELDSKTRKILEILNECHDELKALPMLE 132 Query: 87 DTHLPKTVSGRKRQKVNAETLLDYGRKLSKFSSAPPGYNPETGQDAKAPVHYPWPSEDQM 146 K ++R K+N+ LLDY KLSKF+ PP ++ + A P ++ WP+ED + Sbjct: 133 QVEFEKNTILQQRSKINSTELLDYATKLSKFTKIPPTFD----KGAVGPNNFIWPAEDAL 188 Query: 147 RKTLL 151 R+ +L Sbjct: 189 RRGML 193 >5OQM-h nrPDB Length = 300 Score = 166 bits (419), Expect = 1e-41, Method: Composition-based stats. Identities = 33/141 (23%), Positives = 63/141 (44%), Gaps = 23/141 (16%) Query: 30 LIENWTSLVGHLKTLHSLISNYTNGRELQNEISSLLKQDKELDLQIQDCMREMTS----- 84 LI +L ++K L + Y N +++ + L + +++ + +C E+ Sbjct: 73 LIRTDEALFENVKLLAEYDNIYRNLQKIDKDSEELDSKTRKILEILNECHDELXXXXXXX 132 Query: 85 --------------IYDTHLPKTVSGRKRQKVNAETLLDYGRKLSKFSSAPPGYNPETGQ 130 + K ++R K+N+ LLDY KLSKF+ PP ++ + Sbjct: 133 XXXXXXXXXKALPMLEQVEFEKNTILQQRSKINSTELLDYATKLSKFTKIPPTFD----K 188 Query: 131 DAKAPVHYPWPSEDQMRKTLL 151 A P ++ WP+ED +R+ +L Sbjct: 189 GAVGPNNFIWPAEDALRRGML 209 >4MYG-A nrPDB Length = 372 Score = 32.1 bits (71), Expect = 0.26, Method: Composition-based stats. Identities = 14/53 (26%), Positives = 23/53 (43%) Query: 167 SQLFSEQPPKTNEPTETETEIDANKAVEEKTKMNYPASPTFTTQEENKEVESP 219 +QLF P+ + E E+D +K + + +P F EE E + P Sbjct: 291 TQLFPRASPQAADLLEKMLELDVDKRLTAAQALTHPFFEPFRDPEEETEAQQP 343 >1M7S-A nrPDB Length = 484 Score = 30.9 bits (68), Expect = 0.53, Method: Composition-based stats. Identities = 16/54 (29%), Positives = 26/54 (48%), Gaps = 3/54 (5%) Query: 124 YNPETGQDAKAPVHY---PWPSEDQMRKTLLFQFSTSMVPNLSATASQLFSEQP 174 + ET Q A AP + PSED++ + +F ++ + + L A L QP Sbjct: 299 FFQETEQVAMAPANLVPGIEPSEDRLLQGRVFSYADTQMYRLGANGLSLPVNQP 352 >5G5P-A nrPDB Length = 805 Score = 30.5 bits (67), Expect = 0.62, Method: Composition-based stats. Identities = 18/65 (27%), Positives = 28/65 (43%) Query: 109 DYGRKLSKFSSAPPGYNPETGQDAKAPVHYPWPSEDQMRKTLLFQFSTSMVPNLSATASQ 168 +Y KL++ SS P N + A P H+P+ E + + + S P L+ Sbjct: 582 NYNEKLNQNSSVKPQINTSPKRVATRPNHFPFSQESKQLPQISQSHTLSTNPLLTPQVHG 641 Query: 169 LFSEQ 173 SEQ Sbjct: 642 DLSEQ 646 >5TEY-A nrPDB Length = 598 Score = 30.1 bits (66), Expect = 0.75, Method: Composition-based stats. Identities = 16/65 (24%), Positives = 29/65 (44%), Gaps = 10/65 (15%) Query: 56 ELQNEISSLL-------KQDKELDLQIQDCMREMTSIYDTHLPKTVSGRKRQKVNAETLL 108 ++ EI SLL +Q K++ +I + + T+ + + K R R + + Sbjct: 238 DVDLEIESLLNQQSTKEQQSKKVSQEILELLNTTTAKEQSIVEK---FRSRGRAQVQEFC 294 Query: 109 DYGRK 113 DYG K Sbjct: 295 DYGTK 299 >4UFC-A nrPDB Length = 811 Score = 30.1 bits (66), Expect = 0.94, Method: Composition-based stats. Identities = 28/88 (31%), Positives = 34/88 (38%), Gaps = 4/88 (4%) Query: 87 DTHLPKTV--SGRKRQKVNAETLLDYGRKLSKFSSAPPGYNPETGQDA-KAPVHYPWPSE 143 PK + G A L YGR L SS+ PG P Q H PW S+ Sbjct: 310 QLETPKRIENFGNGEDMAMAALLFHYGRYLL-ISSSQPGGQPANLQGIWNNSTHAPWDSK 368 Query: 144 DQMRKTLLFQFSTSMVPNLSATASQLFS 171 + + + V NLS T S LFS Sbjct: 369 YTININTEMNYWPAEVTNLSETHSPLFS 396 >1YR7-A nrPDB Length = 262 Score = 29.7 bits (65), Expect = 1.2, Method: Composition-based stats. Identities = 15/31 (48%), Positives = 19/31 (61%), Gaps = 2/31 (6%) Query: 3 YQRAIDSIEECLNKQLRLSSEKVDQYVLIEN 33 Y R ++ E LNK LRL EK + YVLI+ Sbjct: 88 YDRLMEKFNEYLNKILRL--EKENDYVLIDT 116 >6I7T-G nrPDB Length = 712 Score = 29.7 bits (65), Expect = 1.2, Method: Composition-based stats. Identities = 17/75 (22%), Positives = 30/75 (40%), Gaps = 7/75 (9%) Query: 71 LDLQIQDCMREMTSIYDTHL--PKTVSGRKRQKVNAETLLDYGRKLSKFSSAPP---GYN 125 LD IQD + S H+ K V + K+ T + G K+ + + G N Sbjct: 308 LDSNIQD--DQTYSYESRHIYKEKDVVLAQSCKIGKCTAIGSGTKIGEGTKIENSVIGRN 365 Query: 126 PETGQDAKAPVHYPW 140 + G++ + + W Sbjct: 366 CQIGENIRIKNSFIW 380 >5FWP-E nrPDB Length = 378 Score = 29.4 bits (64), Expect = 1.4, Method: Composition-based stats. Identities = 28/145 (19%), Positives = 48/145 (33%), Gaps = 27/145 (18%) Query: 54 GRELQNEISSLLKQDKELDLQIQDCMREMTSIYDTHLPKTVSGRKRQKVNAETLLDYGRK 113 + Q E L + +E ++ +C R++ + K E L ++ Sbjct: 40 MEQFQKEKEELDRGCRECKRKVAECQRKLKELEVAE---------GGKAELERLQAEAQQ 90 Query: 114 LSKFSSAPPGYNPETGQDAKAPVHYPWPSEDQMRKTLLFQFSTSMVPNLSATASQLFSEQ 173 L K + + + K PW + + FS SMV + + Sbjct: 91 LRKEER---SWEQKLEEMRKKEKSMPWNVDTLSKDG----FSKSMV-----------NTK 132 Query: 174 PPKTNEPTETETEIDANKAVEEKTK 198 P KT E +E E VE+ K Sbjct: 133 PEKTEEDSEEVREQKHKTFVEKYEK 157 >3FII-A nrPDB Length = 427 Score = 27.8 bits (60), Expect = 4.3, Method: Composition-based stats. Identities = 11/36 (30%), Positives = 20/36 (55%), Gaps = 3/36 (8%) Query: 99 RQKVNAETLLDY---GRKLSKFSSAPPGYNPETGQD 131 ++K+ L +Y +LS+ +SAPP Y+ +D Sbjct: 283 KEKIYNNLLANYEKIATRLSRVNSAPPEYDINEYKD 318 >5CVY-A nrPDB Length = 709 Score = 27.8 bits (60), Expect = 4.4, Method: Composition-based stats. Identities = 23/82 (28%), Positives = 31/82 (37%), Gaps = 2/82 (2%) Query: 102 VNAETLLDYGRKLSKFSSAPPGYNPETGQDAKAPVHYPWPSEDQMRKTLLFQFSTSMVPN 161 V E DY R K P G+N GQ + P PS+ Q ++ + PN Sbjct: 587 VTEEEREDYDRFFKKEVYFPNGWNGTFGQGNQIPGSSTIPSDPQRGGNGVYTSFADLRPN 646 Query: 162 LSATA--SQLFSEQPPKTNEPT 181 + S L S+ NE T Sbjct: 647 IKQDPAWSSLESKYQSSFNEAT 668 >6B3O-A nrPDB Length = 605 Score = 27.8 bits (60), Expect = 4.5, Method: Composition-based stats. Identities = 6/34 (17%), Positives = 19/34 (55%) Query: 42 KTLHSLISNYTNGRELQNEISSLLKQDKELDLQI 75 K S I+ ++++++I + + K+++ +I Sbjct: 549 KLNESYINLIKRMKQIEDKIEEIESKQKKIENEI 582 >2NCA-A nrPDB Length = 128 Score = 27.1 bits (58), Expect = 7.1, Method: Composition-based stats. Identities = 6/36 (16%), Positives = 15/36 (41%) Query: 54 GRELQNEISSLLKQDKELDLQIQDCMREMTSIYDTH 89 + Q E L + +E ++ +C R++ + Sbjct: 42 MEQFQKEKEELDRGCRECKRKVAECQRKLKELEVAE 77 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.307 0.125 0.332 Lambda K H 0.267 0.0391 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,531,048 Number of Sequences: 36641 Number of extensions: 311603 Number of successful extensions: 769 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 17 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 744 Number of HSP's gapped (non-prelim): 33 length of query: 239 length of database: 10,336,785 effective HSP length: 94 effective length of query: 145 effective length of database: 6,892,531 effective search space: 999416995 effective search space used: 999416995 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 57 (26.6 bits)