BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9Y7N2 (138 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5N9J-C nrPDB 286 2e-78 5OQM-m nrPDB 43 5e-05 6OIK-A nrPDB 33 0.040 1BML-C nrPDB 28 1.3 1C4P-A nrPDB 28 1.8 5UDI-A nrPDB 27 2.6 5BR7-A nrPDB 26 4.3 1H78-A nrPDB 26 5.0 6FSZ-KK nrPDB 25 9.4 >5N9J-C nrPDB Length = 138 Score = 286 bits (733), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 138/138 (100%), Positives = 138/138 (100%) Query: 1 MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQ 60 MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQ Sbjct: 1 MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQ 60 Query: 61 DLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVM 120 DLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVM Sbjct: 61 DLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVM 120 Query: 121 GNVSRDSSQPRSPSRRKK 138 GNVSRDSSQPRSPSRRKK Sbjct: 121 GNVSRDSSQPRSPSRRKK 138 >5OQM-m nrPDB Length = 220 Score = 42.7 bits (99), Expect = 5e-05, Method: Compositional matrix adjust. Identities = 24/60 (40%), Positives = 37/60 (61%), Gaps = 2/60 (3%) Query: 7 YHYVG-SVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKR-KMRRSYKAYIQDLPG 64 Y+YV Y +P+ Q+LI +YGL +++++V R + G K K+R+SYK I DL G Sbjct: 14 YYYVDPETTYTYQQPNPLQDLISVYGLDDISRQVARTNLDGTKAVKLRKSYKNQIADLSG 73 >6OIK-A nrPDB Length = 354 Score = 33.1 bits (74), Expect = 0.040, Method: Compositional matrix adjust. Identities = 27/118 (22%), Positives = 52/118 (44%), Gaps = 18/118 (15%) Query: 10 VGSVDYQPTRPSAHQNLIELYGLTELAKKVG----RVDEFGNKRKMRRSYKAYIQD---- 61 +G+ DYQPT + ++ G+ E R+ + G +R R+ + +D Sbjct: 163 IGAADYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAI 222 Query: 62 -----LPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPK-VFG 113 L GY+++L ++ TN + E + + ++ F ++ II N K +FG Sbjct: 223 IFCVALSGYDQVLHEDET----TNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFG 276 >1BML-C nrPDB Length = 362 Score = 28.1 bits (61), Expect = 1.3, Method: Compositional matrix adjust. Identities = 17/64 (26%), Positives = 31/64 (48%) Query: 33 TELAKKVGRVDEFGNKRKMRRSYKAYIQDLPGYNEILRDNTIKQWLTNPIREEVPIDIEF 92 T+L K + D ++ + ++ + PGY RD++I + R +P+D EF Sbjct: 171 TKLLKTLAIGDTITSQELLAQAQSILNKTHPGYTIYERDSSIVTHDNDIFRTILPMDQEF 230 Query: 93 LHHV 96 +HV Sbjct: 231 TYHV 234 >1C4P-A nrPDB Length = 137 Score = 27.7 bits (60), Expect = 1.8, Method: Compositional matrix adjust. Identities = 17/64 (26%), Positives = 31/64 (48%) Query: 33 TELAKKVGRVDEFGNKRKMRRSYKAYIQDLPGYNEILRDNTIKQWLTNPIREEVPIDIEF 92 T+L K + D ++ + ++ + PGY RD++I + R +P+D EF Sbjct: 34 TKLLKTLAIGDTITSQELLAQAQSILNKTHPGYTIYERDSSIVTHDNDIFRTILPMDQEF 93 Query: 93 LHHV 96 +HV Sbjct: 94 TYHV 97 >5UDI-A nrPDB Length = 479 Score = 26.9 bits (58), Expect = 2.6, Method: Composition-based stats. Identities = 17/71 (23%), Positives = 32/71 (45%), Gaps = 12/71 (16%) Query: 1 MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQ 60 M ++ +H+ +V+ +PT AH +L +Y E GN RK +++ + Sbjct: 322 MIRSAIFHFESAVEKKPTFEVAHLDLARMY------------IEAGNHRKAEENFQKLLC 369 Query: 61 DLPGYNEILRD 71 P E ++D Sbjct: 370 MKPVVEETMQD 380 >5BR7-A nrPDB Length = 393 Score = 26.2 bits (56), Expect = 4.3, Method: Compositional matrix adjust. Identities = 12/25 (48%), Positives = 16/25 (64%), Gaps = 2/25 (8%) Query: 63 PGYNEILRDNTIKQWLTNPIREEVP 87 P Y +RD T KQW T+P +E+P Sbjct: 148 PLYEAFIRDYTAKQWQTDP--KELP 170 >1H78-A nrPDB Length = 605 Score = 26.2 bits (56), Expect = 5.0, Method: Compositional matrix adjust. Identities = 11/32 (34%), Positives = 18/32 (56%) Query: 9 YVGSVDYQPTRPSAHQNLIELYGLTELAKKVG 40 +V +DY P P + L++L G+ E K+G Sbjct: 64 HVHDIDYSPALPFTNCCLVDLKGMLENGFKLG 95 >6FSZ-KK nrPDB Length = 733 Score = 25.0 bits (53), Expect = 9.4, Method: Compositional matrix adjust. Identities = 13/45 (28%), Positives = 24/45 (53%), Gaps = 3/45 (6%) Query: 86 VPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVMGNVSRDSSQP 130 VP+ +E + +V+P P +P++ L+D VV+ + QP Sbjct: 626 VPVKLE---EIKTVDPASAPNHSPEIDNLDDLVVLKKKNIQKKQP 667 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5N9J-C nrPDB 273 2e-74 5OQM-m nrPDB 94 3e-20 Sequences not found previously or not previously below threshold: 6OIK-A nrPDB 29 0.77 1J0D-A nrPDB 29 0.82 3V7C-A nrPDB 28 1.2 5BR7-A nrPDB 28 1.9 1BML-C nrPDB 27 2.0 4MHZ-A nrPDB 27 2.3 1C4P-A nrPDB 27 3.0 6FSZ-KK nrPDB 27 3.0 5K36-J nrPDB 27 3.0 2YGT-A nrPDB 27 3.2 3I5P-A nrPDB 26 4.6 6FKI-F nrPDB 26 5.2 4QFK-H nrPDB 26 5.2 3V8X-A nrPDB 26 6.0 CONVERGED! >5N9J-C nrPDB Length = 138 Score = 273 bits (697), Expect = 2e-74, Method: Composition-based stats. Identities = 138/138 (100%), Positives = 138/138 (100%) Query: 1 MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQ 60 MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQ Sbjct: 1 MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQ 60 Query: 61 DLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVM 120 DLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVM Sbjct: 61 DLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVM 120 Query: 121 GNVSRDSSQPRSPSRRKK 138 GNVSRDSSQPRSPSRRKK Sbjct: 121 GNVSRDSSQPRSPSRRKK 138 >5OQM-m nrPDB Length = 220 Score = 93.6 bits (231), Expect = 3e-20, Method: Composition-based stats. Identities = 24/60 (40%), Positives = 37/60 (61%), Gaps = 2/60 (3%) Query: 7 YHYVG-SVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKR-KMRRSYKAYIQDLPG 64 Y+YV Y +P+ Q+LI +YGL +++++V R + G K K+R+SYK I DL G Sbjct: 14 YYYVDPETTYTYQQPNPLQDLISVYGLDDISRQVARTNLDGTKAVKLRKSYKNQIADLSG 73 >6OIK-A nrPDB Length = 354 Score = 28.8 bits (63), Expect = 0.77, Method: Composition-based stats. Identities = 27/118 (22%), Positives = 52/118 (44%), Gaps = 18/118 (15%) Query: 10 VGSVDYQPTRPSAHQNLIELYGLTELAKKVG----RVDEFGNKRKMRRSYKAYIQD---- 61 +G+ DYQPT + ++ G+ E R+ + G +R R+ + +D Sbjct: 163 IGAADYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAI 222 Query: 62 -----LPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPK-VFG 113 L GY+++L ++ TN + E + + ++ F ++ II N K +FG Sbjct: 223 IFCVALSGYDQVLHEDET----TNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFG 276 >1J0D-A nrPDB Length = 341 Score = 28.8 bits (63), Expect = 0.82, Method: Composition-based stats. Identities = 33/137 (24%), Positives = 57/137 (41%), Gaps = 22/137 (16%) Query: 20 PSAHQNLIELYGLTELAKKVG---RVDEFGNKRKMRRSYKAYIQDL--PGYNEILRDNTI 74 P A +++ G EL++ +G RV E G MR+S+ +Q+L G+ Sbjct: 106 PEAEKDVYNRVGNIELSRIMGADVRVIEDGFDIGMRKSFANALQELEDAGHKPYPIPAGC 165 Query: 75 KQ----------WLTNPIREEVPIDIEFLHHVF-----SVEPGIIPGFNPKVFGLEDDVV 119 + + I +EV + I+F V S GI+ G +G +DDV+ Sbjct: 166 SEHKYGGLGFVGFADEVINQEVELGIKFDKIVVCCVTGSTTAGILAGMAQ--YGRQDDVI 223 Query: 120 MGNVSRDSSQPRSPSRR 136 + S S + + + R Sbjct: 224 AIDASFTSEKTKEQTLR 240 >3V7C-A nrPDB Length = 329 Score = 28.1 bits (61), Expect = 1.2, Method: Composition-based stats. Identities = 13/49 (26%), Positives = 23/49 (46%), Gaps = 1/49 (2%) Query: 1 MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKR 49 + Q P+ Y G +D Q T+ SA + E+Y + + + GN+ Sbjct: 60 LQQLPDIWYQGIID-QYTKSSALFDFSEVYDSIDSTQLAAKKSLVGNQS 107 >5BR7-A nrPDB Length = 393 Score = 27.7 bits (60), Expect = 1.9, Method: Composition-based stats. Identities = 12/25 (48%), Positives = 16/25 (64%), Gaps = 2/25 (8%) Query: 63 PGYNEILRDNTIKQWLTNPIREEVP 87 P Y +RD T KQW T+P +E+P Sbjct: 148 PLYEAFIRDYTAKQWQTDP--KELP 170 >1BML-C nrPDB Length = 362 Score = 27.3 bits (59), Expect = 2.0, Method: Composition-based stats. Identities = 19/85 (22%), Positives = 35/85 (41%) Query: 12 SVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQDLPGYNEILRD 71 Q T + + T+L K + D ++ + ++ + PGY RD Sbjct: 150 EYTVQFTPLNPDDDFRPGLKDTKLLKTLAIGDTITSQELLAQAQSILNKTHPGYTIYERD 209 Query: 72 NTIKQWLTNPIREEVPIDIEFLHHV 96 ++I + R +P+D EF +HV Sbjct: 210 SSIVTHDNDIFRTILPMDQEFTYHV 234 >4MHZ-A nrPDB Length = 326 Score = 27.3 bits (59), Expect = 2.3, Method: Composition-based stats. Identities = 12/51 (23%), Positives = 22/51 (43%), Gaps = 1/51 (1%) Query: 2 AQAPEYH-YVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKM 51 A+ P ++ Y G R ++ + GL EL ++ R + F N + Sbjct: 215 AENPRFYSYFGETQPVYRRLVNIESRLNDAGLMELPRRRRRTNYFSNSSTV 265 >1C4P-A nrPDB Length = 137 Score = 26.9 bits (58), Expect = 3.0, Method: Composition-based stats. Identities = 19/85 (22%), Positives = 35/85 (41%) Query: 12 SVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQDLPGYNEILRD 71 Q T + + T+L K + D ++ + ++ + PGY RD Sbjct: 13 EYTVQFTPLNPDDDFRPGLKDTKLLKTLAIGDTITSQELLAQAQSILNKTHPGYTIYERD 72 Query: 72 NTIKQWLTNPIREEVPIDIEFLHHV 96 ++I + R +P+D EF +HV Sbjct: 73 SSIVTHDNDIFRTILPMDQEFTYHV 97 >6FSZ-KK nrPDB Length = 733 Score = 26.9 bits (58), Expect = 3.0, Method: Composition-based stats. Identities = 14/52 (26%), Positives = 29/52 (55%), Gaps = 6/52 (11%) Query: 86 VPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVMGNVSRDSSQPRSPSRRK 137 VP+ +E + +V+P P +P++ L+D VV+ + + Q + P++ K Sbjct: 626 VPVKLE---EIKTVDPASAPNHSPEIDNLDDLVVL---KKKNIQKKQPAKEK 671 >5K36-J nrPDB Length = 559 Score = 26.9 bits (58), Expect = 3.0, Method: Composition-based stats. Identities = 14/52 (26%), Positives = 29/52 (55%), Gaps = 6/52 (11%) Query: 86 VPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVMGNVSRDSSQPRSPSRRK 137 VP+ +E + +V+P P +P++ L+D VV+ + + Q + P++ K Sbjct: 501 VPVKLE---EIKTVDPASAPNHSPEIDNLDDLVVL---KKKNIQKKQPAKEK 546 >2YGT-A nrPDB Length = 298 Score = 26.9 bits (58), Expect = 3.2, Method: Composition-based stats. Identities = 18/79 (22%), Positives = 34/79 (43%), Gaps = 6/79 (7%) Query: 42 VDEFGNKRKMRRSYKAYIQDLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEP 101 ++ G+K ++R+ + + N+ +R + T ++ +D F+ FS E Sbjct: 2 SNDLGSKSEIRKEENGNVTIITQNNKQIRKYSSTDSATTKSNSKITVDASFVDDKFSSEM 61 Query: 102 GII---PGFNP---KVFGL 114 I GF P K+F L Sbjct: 62 TTIISLKGFIPSGRKIFAL 80 >3I5P-A nrPDB Length = 525 Score = 26.1 bits (56), Expect = 4.6, Method: Composition-based stats. Identities = 15/54 (27%), Positives = 29/54 (53%), Gaps = 5/54 (9%) Query: 38 KVGRVDEFGNKRKMRRSYKAYIQDLPGYNEILRDNTIKQWLTNPIREEVPIDIE 91 ++ ++ K + ++SY+A ++ YN+ L + WL + REE +DIE Sbjct: 153 QISISNDDEVKLR-QKSYEAALK----YNDRLFHYHMYDWLVSQNREEKLLDIE 201 >6FKI-F nrPDB Length = 498 Score = 26.1 bits (56), Expect = 5.2, Method: Composition-based stats. Identities = 9/32 (28%), Positives = 15/32 (46%) Query: 7 YHYVGSVDYQPTRPSAHQNLIELYGLTELAKK 38 Y V R Q++I + GL EL+++ Sbjct: 385 YEIAQRVKETLQRYKELQDIIAILGLDELSEE 416 >4QFK-H nrPDB Length = 541 Score = 26.1 bits (56), Expect = 5.2, Method: Composition-based stats. Identities = 10/57 (17%), Positives = 27/57 (47%), Gaps = 9/57 (15%) Query: 7 YHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQDLP 63 YH+VG +Y + G+ +L +K+ RV + + ++ + +++ ++ Sbjct: 132 YHFVGDANYPYFQS---------VGIDQLIRKIVRVSDHQVRFELFNAESSFLANMA 179 >3V8X-A nrPDB Length = 904 Score = 25.8 bits (55), Expect = 6.0, Method: Composition-based stats. Identities = 14/60 (23%), Positives = 27/60 (45%), Gaps = 4/60 (6%) Query: 21 SAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQDLPGYNEILRDNTIKQWLTN 80 S + + EL G ++ + + K RR+ YI D+ GY + + T++ + N Sbjct: 802 SKAKEITELLG----SRALLNGNSRNTKATARRTRPWYIVDVSGYYTVKKHFTLRAGVYN 857 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.314 0.137 0.387 Lambda K H 0.267 0.0421 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,325,462 Number of Sequences: 36641 Number of extensions: 262220 Number of successful extensions: 570 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 15 Number of HSP's that attempted gapping in prelim test: 548 Number of HSP's gapped (non-prelim): 35 length of query: 138 length of database: 10,336,785 effective HSP length: 87 effective length of query: 51 effective length of database: 7,149,018 effective search space: 364599918 effective search space used: 364599918 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.4 bits) S2: 54 (25.4 bits)