BLASTP 2.2.23 [Feb-03-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= Q9Y7N2
(138 letters)
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
36,641 sequences; 10,336,785 total letters
Searching..................................................done
Results from round 1
Score E
Sequences producing significant alignments: (bits) Value
5N9J-C nrPDB 286 2e-78
5OQM-m nrPDB 43 5e-05
6OIK-A nrPDB 33 0.040
1BML-C nrPDB 28 1.3
1C4P-A nrPDB 28 1.8
5UDI-A nrPDB 27 2.6
5BR7-A nrPDB 26 4.3
1H78-A nrPDB 26 5.0
6FSZ-KK nrPDB 25 9.4
>5N9J-C nrPDB
Length = 138
Score = 286 bits (733), Expect = 2e-78, Method: Compositional matrix adjust.
Identities = 138/138 (100%), Positives = 138/138 (100%)
Query: 1 MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQ 60
MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQ
Sbjct: 1 MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQ 60
Query: 61 DLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVM 120
DLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVM
Sbjct: 61 DLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVM 120
Query: 121 GNVSRDSSQPRSPSRRKK 138
GNVSRDSSQPRSPSRRKK
Sbjct: 121 GNVSRDSSQPRSPSRRKK 138
>5OQM-m nrPDB
Length = 220
Score = 42.7 bits (99), Expect = 5e-05, Method: Compositional matrix adjust.
Identities = 24/60 (40%), Positives = 37/60 (61%), Gaps = 2/60 (3%)
Query: 7 YHYVG-SVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKR-KMRRSYKAYIQDLPG 64
Y+YV Y +P+ Q+LI +YGL +++++V R + G K K+R+SYK I DL G
Sbjct: 14 YYYVDPETTYTYQQPNPLQDLISVYGLDDISRQVARTNLDGTKAVKLRKSYKNQIADLSG 73
>6OIK-A nrPDB
Length = 354
Score = 33.1 bits (74), Expect = 0.040, Method: Compositional matrix adjust.
Identities = 27/118 (22%), Positives = 52/118 (44%), Gaps = 18/118 (15%)
Query: 10 VGSVDYQPTRPSAHQNLIELYGLTELAKKVG----RVDEFGNKRKMRRSYKAYIQD---- 61
+G+ DYQPT + ++ G+ E R+ + G +R R+ + +D
Sbjct: 163 IGAADYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAI 222
Query: 62 -----LPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPK-VFG 113
L GY+++L ++ TN + E + + ++ F ++ II N K +FG
Sbjct: 223 IFCVALSGYDQVLHEDET----TNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFG 276
>1BML-C nrPDB
Length = 362
Score = 28.1 bits (61), Expect = 1.3, Method: Compositional matrix adjust.
Identities = 17/64 (26%), Positives = 31/64 (48%)
Query: 33 TELAKKVGRVDEFGNKRKMRRSYKAYIQDLPGYNEILRDNTIKQWLTNPIREEVPIDIEF 92
T+L K + D ++ + ++ + PGY RD++I + R +P+D EF
Sbjct: 171 TKLLKTLAIGDTITSQELLAQAQSILNKTHPGYTIYERDSSIVTHDNDIFRTILPMDQEF 230
Query: 93 LHHV 96
+HV
Sbjct: 231 TYHV 234
>1C4P-A nrPDB
Length = 137
Score = 27.7 bits (60), Expect = 1.8, Method: Compositional matrix adjust.
Identities = 17/64 (26%), Positives = 31/64 (48%)
Query: 33 TELAKKVGRVDEFGNKRKMRRSYKAYIQDLPGYNEILRDNTIKQWLTNPIREEVPIDIEF 92
T+L K + D ++ + ++ + PGY RD++I + R +P+D EF
Sbjct: 34 TKLLKTLAIGDTITSQELLAQAQSILNKTHPGYTIYERDSSIVTHDNDIFRTILPMDQEF 93
Query: 93 LHHV 96
+HV
Sbjct: 94 TYHV 97
>5UDI-A nrPDB
Length = 479
Score = 26.9 bits (58), Expect = 2.6, Method: Composition-based stats.
Identities = 17/71 (23%), Positives = 32/71 (45%), Gaps = 12/71 (16%)
Query: 1 MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQ 60
M ++ +H+ +V+ +PT AH +L +Y E GN RK +++ +
Sbjct: 322 MIRSAIFHFESAVEKKPTFEVAHLDLARMY------------IEAGNHRKAEENFQKLLC 369
Query: 61 DLPGYNEILRD 71
P E ++D
Sbjct: 370 MKPVVEETMQD 380
>5BR7-A nrPDB
Length = 393
Score = 26.2 bits (56), Expect = 4.3, Method: Compositional matrix adjust.
Identities = 12/25 (48%), Positives = 16/25 (64%), Gaps = 2/25 (8%)
Query: 63 PGYNEILRDNTIKQWLTNPIREEVP 87
P Y +RD T KQW T+P +E+P
Sbjct: 148 PLYEAFIRDYTAKQWQTDP--KELP 170
>1H78-A nrPDB
Length = 605
Score = 26.2 bits (56), Expect = 5.0, Method: Compositional matrix adjust.
Identities = 11/32 (34%), Positives = 18/32 (56%)
Query: 9 YVGSVDYQPTRPSAHQNLIELYGLTELAKKVG 40
+V +DY P P + L++L G+ E K+G
Sbjct: 64 HVHDIDYSPALPFTNCCLVDLKGMLENGFKLG 95
>6FSZ-KK nrPDB
Length = 733
Score = 25.0 bits (53), Expect = 9.4, Method: Compositional matrix adjust.
Identities = 13/45 (28%), Positives = 24/45 (53%), Gaps = 3/45 (6%)
Query: 86 VPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVMGNVSRDSSQP 130
VP+ +E + +V+P P +P++ L+D VV+ + QP
Sbjct: 626 VPVKLE---EIKTVDPASAPNHSPEIDNLDDLVVLKKKNIQKKQP 667
Searching..................................................done
Results from round 2
Score E
Sequences producing significant alignments: (bits) Value
Sequences used in model and found again:
5N9J-C nrPDB 273 2e-74
5OQM-m nrPDB 94 3e-20
Sequences not found previously or not previously below threshold:
6OIK-A nrPDB 29 0.77
1J0D-A nrPDB 29 0.82
3V7C-A nrPDB 28 1.2
5BR7-A nrPDB 28 1.9
1BML-C nrPDB 27 2.0
4MHZ-A nrPDB 27 2.3
1C4P-A nrPDB 27 3.0
6FSZ-KK nrPDB 27 3.0
5K36-J nrPDB 27 3.0
2YGT-A nrPDB 27 3.2
3I5P-A nrPDB 26 4.6
6FKI-F nrPDB 26 5.2
4QFK-H nrPDB 26 5.2
3V8X-A nrPDB 26 6.0
CONVERGED!
>5N9J-C nrPDB
Length = 138
Score = 273 bits (697), Expect = 2e-74, Method: Composition-based stats.
Identities = 138/138 (100%), Positives = 138/138 (100%)
Query: 1 MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQ 60
MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQ
Sbjct: 1 MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQ 60
Query: 61 DLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVM 120
DLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVM
Sbjct: 61 DLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVM 120
Query: 121 GNVSRDSSQPRSPSRRKK 138
GNVSRDSSQPRSPSRRKK
Sbjct: 121 GNVSRDSSQPRSPSRRKK 138
>5OQM-m nrPDB
Length = 220
Score = 93.6 bits (231), Expect = 3e-20, Method: Composition-based stats.
Identities = 24/60 (40%), Positives = 37/60 (61%), Gaps = 2/60 (3%)
Query: 7 YHYVG-SVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKR-KMRRSYKAYIQDLPG 64
Y+YV Y +P+ Q+LI +YGL +++++V R + G K K+R+SYK I DL G
Sbjct: 14 YYYVDPETTYTYQQPNPLQDLISVYGLDDISRQVARTNLDGTKAVKLRKSYKNQIADLSG 73
>6OIK-A nrPDB
Length = 354
Score = 28.8 bits (63), Expect = 0.77, Method: Composition-based stats.
Identities = 27/118 (22%), Positives = 52/118 (44%), Gaps = 18/118 (15%)
Query: 10 VGSVDYQPTRPSAHQNLIELYGLTELAKKVG----RVDEFGNKRKMRRSYKAYIQD---- 61
+G+ DYQPT + ++ G+ E R+ + G +R R+ + +D
Sbjct: 163 IGAADYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAI 222
Query: 62 -----LPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEPGIIPGFNPK-VFG 113
L GY+++L ++ TN + E + + ++ F ++ II N K +FG
Sbjct: 223 IFCVALSGYDQVLHEDET----TNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFG 276
>1J0D-A nrPDB
Length = 341
Score = 28.8 bits (63), Expect = 0.82, Method: Composition-based stats.
Identities = 33/137 (24%), Positives = 57/137 (41%), Gaps = 22/137 (16%)
Query: 20 PSAHQNLIELYGLTELAKKVG---RVDEFGNKRKMRRSYKAYIQDL--PGYNEILRDNTI 74
P A +++ G EL++ +G RV E G MR+S+ +Q+L G+
Sbjct: 106 PEAEKDVYNRVGNIELSRIMGADVRVIEDGFDIGMRKSFANALQELEDAGHKPYPIPAGC 165
Query: 75 KQ----------WLTNPIREEVPIDIEFLHHVF-----SVEPGIIPGFNPKVFGLEDDVV 119
+ + I +EV + I+F V S GI+ G +G +DDV+
Sbjct: 166 SEHKYGGLGFVGFADEVINQEVELGIKFDKIVVCCVTGSTTAGILAGMAQ--YGRQDDVI 223
Query: 120 MGNVSRDSSQPRSPSRR 136
+ S S + + + R
Sbjct: 224 AIDASFTSEKTKEQTLR 240
>3V7C-A nrPDB
Length = 329
Score = 28.1 bits (61), Expect = 1.2, Method: Composition-based stats.
Identities = 13/49 (26%), Positives = 23/49 (46%), Gaps = 1/49 (2%)
Query: 1 MAQAPEYHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKR 49
+ Q P+ Y G +D Q T+ SA + E+Y + + + GN+
Sbjct: 60 LQQLPDIWYQGIID-QYTKSSALFDFSEVYDSIDSTQLAAKKSLVGNQS 107
>5BR7-A nrPDB
Length = 393
Score = 27.7 bits (60), Expect = 1.9, Method: Composition-based stats.
Identities = 12/25 (48%), Positives = 16/25 (64%), Gaps = 2/25 (8%)
Query: 63 PGYNEILRDNTIKQWLTNPIREEVP 87
P Y +RD T KQW T+P +E+P
Sbjct: 148 PLYEAFIRDYTAKQWQTDP--KELP 170
>1BML-C nrPDB
Length = 362
Score = 27.3 bits (59), Expect = 2.0, Method: Composition-based stats.
Identities = 19/85 (22%), Positives = 35/85 (41%)
Query: 12 SVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQDLPGYNEILRD 71
Q T + + T+L K + D ++ + ++ + PGY RD
Sbjct: 150 EYTVQFTPLNPDDDFRPGLKDTKLLKTLAIGDTITSQELLAQAQSILNKTHPGYTIYERD 209
Query: 72 NTIKQWLTNPIREEVPIDIEFLHHV 96
++I + R +P+D EF +HV
Sbjct: 210 SSIVTHDNDIFRTILPMDQEFTYHV 234
>4MHZ-A nrPDB
Length = 326
Score = 27.3 bits (59), Expect = 2.3, Method: Composition-based stats.
Identities = 12/51 (23%), Positives = 22/51 (43%), Gaps = 1/51 (1%)
Query: 2 AQAPEYH-YVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKM 51
A+ P ++ Y G R ++ + GL EL ++ R + F N +
Sbjct: 215 AENPRFYSYFGETQPVYRRLVNIESRLNDAGLMELPRRRRRTNYFSNSSTV 265
>1C4P-A nrPDB
Length = 137
Score = 26.9 bits (58), Expect = 3.0, Method: Composition-based stats.
Identities = 19/85 (22%), Positives = 35/85 (41%)
Query: 12 SVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQDLPGYNEILRD 71
Q T + + T+L K + D ++ + ++ + PGY RD
Sbjct: 13 EYTVQFTPLNPDDDFRPGLKDTKLLKTLAIGDTITSQELLAQAQSILNKTHPGYTIYERD 72
Query: 72 NTIKQWLTNPIREEVPIDIEFLHHV 96
++I + R +P+D EF +HV
Sbjct: 73 SSIVTHDNDIFRTILPMDQEFTYHV 97
>6FSZ-KK nrPDB
Length = 733
Score = 26.9 bits (58), Expect = 3.0, Method: Composition-based stats.
Identities = 14/52 (26%), Positives = 29/52 (55%), Gaps = 6/52 (11%)
Query: 86 VPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVMGNVSRDSSQPRSPSRRK 137
VP+ +E + +V+P P +P++ L+D VV+ + + Q + P++ K
Sbjct: 626 VPVKLE---EIKTVDPASAPNHSPEIDNLDDLVVL---KKKNIQKKQPAKEK 671
>5K36-J nrPDB
Length = 559
Score = 26.9 bits (58), Expect = 3.0, Method: Composition-based stats.
Identities = 14/52 (26%), Positives = 29/52 (55%), Gaps = 6/52 (11%)
Query: 86 VPIDIEFLHHVFSVEPGIIPGFNPKVFGLEDDVVMGNVSRDSSQPRSPSRRK 137
VP+ +E + +V+P P +P++ L+D VV+ + + Q + P++ K
Sbjct: 501 VPVKLE---EIKTVDPASAPNHSPEIDNLDDLVVL---KKKNIQKKQPAKEK 546
>2YGT-A nrPDB
Length = 298
Score = 26.9 bits (58), Expect = 3.2, Method: Composition-based stats.
Identities = 18/79 (22%), Positives = 34/79 (43%), Gaps = 6/79 (7%)
Query: 42 VDEFGNKRKMRRSYKAYIQDLPGYNEILRDNTIKQWLTNPIREEVPIDIEFLHHVFSVEP 101
++ G+K ++R+ + + N+ +R + T ++ +D F+ FS E
Sbjct: 2 SNDLGSKSEIRKEENGNVTIITQNNKQIRKYSSTDSATTKSNSKITVDASFVDDKFSSEM 61
Query: 102 GII---PGFNP---KVFGL 114
I GF P K+F L
Sbjct: 62 TTIISLKGFIPSGRKIFAL 80
>3I5P-A nrPDB
Length = 525
Score = 26.1 bits (56), Expect = 4.6, Method: Composition-based stats.
Identities = 15/54 (27%), Positives = 29/54 (53%), Gaps = 5/54 (9%)
Query: 38 KVGRVDEFGNKRKMRRSYKAYIQDLPGYNEILRDNTIKQWLTNPIREEVPIDIE 91
++ ++ K + ++SY+A ++ YN+ L + WL + REE +DIE
Sbjct: 153 QISISNDDEVKLR-QKSYEAALK----YNDRLFHYHMYDWLVSQNREEKLLDIE 201
>6FKI-F nrPDB
Length = 498
Score = 26.1 bits (56), Expect = 5.2, Method: Composition-based stats.
Identities = 9/32 (28%), Positives = 15/32 (46%)
Query: 7 YHYVGSVDYQPTRPSAHQNLIELYGLTELAKK 38
Y V R Q++I + GL EL+++
Sbjct: 385 YEIAQRVKETLQRYKELQDIIAILGLDELSEE 416
>4QFK-H nrPDB
Length = 541
Score = 26.1 bits (56), Expect = 5.2, Method: Composition-based stats.
Identities = 10/57 (17%), Positives = 27/57 (47%), Gaps = 9/57 (15%)
Query: 7 YHYVGSVDYQPTRPSAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQDLP 63
YH+VG +Y + G+ +L +K+ RV + + ++ + +++ ++
Sbjct: 132 YHFVGDANYPYFQS---------VGIDQLIRKIVRVSDHQVRFELFNAESSFLANMA 179
>3V8X-A nrPDB
Length = 904
Score = 25.8 bits (55), Expect = 6.0, Method: Composition-based stats.
Identities = 14/60 (23%), Positives = 27/60 (45%), Gaps = 4/60 (6%)
Query: 21 SAHQNLIELYGLTELAKKVGRVDEFGNKRKMRRSYKAYIQDLPGYNEILRDNTIKQWLTN 80
S + + EL G ++ + + K RR+ YI D+ GY + + T++ + N
Sbjct: 802 SKAKEITELLG----SRALLNGNSRNTKATARRTRPWYIVDVSGYYTVKKHFTLRAGVYN 857
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
Posted date: Jan 3, 2023 9:05 PM
Number of letters in database: 10,336,785
Number of sequences in database: 36,641
Lambda K H
0.314 0.137 0.387
Lambda K H
0.267 0.0421 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,325,462
Number of Sequences: 36641
Number of extensions: 262220
Number of successful extensions: 570
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 548
Number of HSP's gapped (non-prelim): 35
length of query: 138
length of database: 10,336,785
effective HSP length: 87
effective length of query: 51
effective length of database: 7,149,018
effective search space: 364599918
effective search space used: 364599918
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.4 bits)
S2: 54 (25.4 bits)