BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9Y6H6 (103 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2NDJ-A nrPDB 216 2e-57 2K21-A nrPDB 39 4e-04 2M0Q-A nrPDB 33 0.021 >2NDJ-A nrPDB Length = 112 Score = 216 bits (551), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 103/103 (100%), Positives = 103/103 (100%) Query: 1 METTNGTETWYESLHAVLKALNATLHSNLLCRPGPGLGPDNQTEERRASLPGRDDNSYMY 60 METTNGTETWYESLHAVLKALNATLHSNLLCRPGPGLGPDNQTEERRASLPGRDDNSYMY Sbjct: 10 METTNGTETWYESLHAVLKALNATLHSNLLCRPGPGLGPDNQTEERRASLPGRDDNSYMY 69 Query: 61 ILFVMFLFAVTVGSLILGYTRSRKVDKRSDPYHVYIKNRVSMI 103 ILFVMFLFAVTVGSLILGYTRSRKVDKRSDPYHVYIKNRVSMI Sbjct: 70 ILFVMFLFAVTVGSLILGYTRSRKVDKRSDPYHVYIKNRVSMI 112 >2K21-A nrPDB Length = 138 Score = 39.3 bits (90), Expect = 4e-04, Method: Compositional matrix adjust. Identities = 17/53 (32%), Positives = 31/53 (58%) Query: 46 RRASLPGRDDNSYMYILFVMFLFAVTVGSLILGYTRSRKVDKRSDPYHVYIKN 98 RR+ G +Y+L V+ F ++L Y RS+K++ +DP++VYI++ Sbjct: 41 RRSPRSGDGKLEALYVLMVLGFFGFFTLGIMLSYIRSKKLEHSNDPFNVYIES 93 >2M0Q-A nrPDB Length = 123 Score = 33.5 bits (75), Expect = 0.021, Method: Compositional matrix adjust. Identities = 18/61 (29%), Positives = 34/61 (55%), Gaps = 4/61 (6%) Query: 40 DNQTEERRASLPGRD-DNSYMYILFVMFL---FAVTVGSLILGYTRSRKVDKRSDPYHVY 95 N T E+ A D +N Y IL++M + F+ + ++++ +S++ + +DPYH Y Sbjct: 28 QNTTAEQEALQAKVDAENFYYVILYLMVMIGMFSFIIVAILVSTVKSKRREHSNDPYHQY 87 Query: 96 I 96 I Sbjct: 88 I 88 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2NDJ-A nrPDB 201 7e-53 2K21-A nrPDB 95 5e-21 Sequences not found previously or not previously below threshold: 2M0Q-A nrPDB 46 4e-06 1XTP-A nrPDB 27 2.2 >2NDJ-A nrPDB Length = 112 Score = 201 bits (511), Expect = 7e-53, Method: Composition-based stats. Identities = 103/103 (100%), Positives = 103/103 (100%) Query: 1 METTNGTETWYESLHAVLKALNATLHSNLLCRPGPGLGPDNQTEERRASLPGRDDNSYMY 60 METTNGTETWYESLHAVLKALNATLHSNLLCRPGPGLGPDNQTEERRASLPGRDDNSYMY Sbjct: 10 METTNGTETWYESLHAVLKALNATLHSNLLCRPGPGLGPDNQTEERRASLPGRDDNSYMY 69 Query: 61 ILFVMFLFAVTVGSLILGYTRSRKVDKRSDPYHVYIKNRVSMI 103 ILFVMFLFAVTVGSLILGYTRSRKVDKRSDPYHVYIKNRVSMI Sbjct: 70 ILFVMFLFAVTVGSLILGYTRSRKVDKRSDPYHVYIKNRVSMI 112 >2K21-A nrPDB Length = 138 Score = 95.5 bits (236), Expect = 5e-21, Method: Composition-based stats. Identities = 17/53 (32%), Positives = 31/53 (58%) Query: 46 RRASLPGRDDNSYMYILFVMFLFAVTVGSLILGYTRSRKVDKRSDPYHVYIKN 98 RR+ G +Y+L V+ F ++L Y RS+K++ +DP++VYI++ Sbjct: 41 RRSPRSGDGKLEALYVLMVLGFFGFFTLGIMLSYIRSKKLEHSNDPFNVYIES 93 >2M0Q-A nrPDB Length = 123 Score = 45.8 bits (107), Expect = 4e-06, Method: Composition-based stats. Identities = 9/38 (23%), Positives = 26/38 (68%) Query: 59 MYILFVMFLFAVTVGSLILGYTRSRKVDKRSDPYHVYI 96 +Y++ ++ +F+ + ++++ +S++ + +DPYH YI Sbjct: 51 LYLMVMIGMFSFIIVAILVSTVKSKRREHSNDPYHQYI 88 >1XTP-A nrPDB Length = 254 Score = 26.9 bits (58), Expect = 2.2, Method: Composition-based stats. Identities = 10/27 (37%), Positives = 12/27 (44%) Query: 34 GPGLGPDNQTEERRASLPGRDDNSYMY 60 GPG P + R + GRD N Y Sbjct: 1 GPGSMPSKEASSRNLPISGRDTNGKTY 27 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.137 0.390 Lambda K H 0.267 0.0422 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,177,248 Number of Sequences: 36641 Number of extensions: 143571 Number of successful extensions: 282 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 272 Number of HSP's gapped (non-prelim): 10 length of query: 103 length of database: 10,336,785 effective HSP length: 70 effective length of query: 33 effective length of database: 7,771,915 effective search space: 256473195 effective search space used: 256473195 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 53 (25.0 bits)