BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9Y4X3 (112 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2KUM-A nrPDB 183 1e-47 6CWS-A nrPDB 65 8e-12 3UY5-A nrPDB 27 2.8 >2KUM-A nrPDB Length = 88 Score = 183 bits (465), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 88/88 (100%), Positives = 88/88 (100%) Query: 25 FLLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSICIHPQNPS 84 FLLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSICIHPQNPS Sbjct: 1 FLLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSICIHPQNPS 60 Query: 85 LSQWFEHQERKLHGTLPKLNFGMLRKMG 112 LSQWFEHQERKLHGTLPKLNFGMLRKMG Sbjct: 61 LSQWFEHQERKLHGTLPKLNFGMLRKMG 88 >6CWS-A nrPDB Length = 108 Score = 64.7 bits (156), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 29/73 (39%), Positives = 47/73 (64%), Gaps = 1/73 (1%) Query: 26 LLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSICIHPQNPSL 85 +LP +++CCT++ +S +LL +V +Q ADGDC L A +LH+ +R IC+ P N ++ Sbjct: 4 ILPIASSCCTEVSH-HISRRLLERVNMCRIQRADGDCDLAAVILHVKRRRICVSPHNHTV 62 Query: 86 SQWFEHQERKLHG 98 QW + Q K +G Sbjct: 63 KQWMKVQAAKKNG 75 >3UY5-A nrPDB Length = 428 Score = 26.6 bits (57), Expect = 2.8, Method: Composition-based stats. Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 3/50 (6%) Query: 37 LYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHL---AQRSICIHPQNP 83 LYR +D L +V ++ AD C L ++ L + SI HPQ+P Sbjct: 251 LYRVDRTDLKLARVSELRAVTADAHCALWRALIGLDSMERISIITHPQDP 300 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2KUM-A nrPDB 152 3e-38 6CWS-A nrPDB 121 6e-29 Sequences not found previously or not previously below threshold: 1HA6-A nrPDB 31 0.098 5LTL-A nrPDB 30 0.20 5CBA-E nrPDB 29 0.56 1G2S-A nrPDB 28 0.73 2RA4-A nrPDB 28 1.0 5A9Q-2 nrPDB 27 1.8 2JYO-A nrPDB 27 3.0 1NCV-A nrPDB 25 5.6 4L4I-A nrPDB 25 6.6 3IM6-A nrPDB 25 6.6 2Q0T-A nrPDB 25 7.0 1NR4-A nrPDB 25 7.0 4X2Q-A nrPDB 25 7.9 5BPZ-A nrPDB 25 8.2 3FSR-A nrPDB 25 9.7 3FPC-A nrPDB 25 9.7 1YKF-A nrPDB 25 9.7 CONVERGED! >2KUM-A nrPDB Length = 88 Score = 152 bits (385), Expect = 3e-38, Method: Composition-based stats. Identities = 88/88 (100%), Positives = 88/88 (100%) Query: 25 FLLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSICIHPQNPS 84 FLLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSICIHPQNPS Sbjct: 1 FLLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSICIHPQNPS 60 Query: 85 LSQWFEHQERKLHGTLPKLNFGMLRKMG 112 LSQWFEHQERKLHGTLPKLNFGMLRKMG Sbjct: 61 LSQWFEHQERKLHGTLPKLNFGMLRKMG 88 >6CWS-A nrPDB Length = 108 Score = 121 bits (304), Expect = 6e-29, Method: Composition-based stats. Identities = 29/73 (39%), Positives = 47/73 (64%), Gaps = 1/73 (1%) Query: 26 LLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSICIHPQNPSL 85 +LP +++CCT++ +S +LL +V +Q ADGDC L A +LH+ +R IC+ P N ++ Sbjct: 4 ILPIASSCCTEVSH-HISRRLLERVNMCRIQRADGDCDLAAVILHVKRRRICVSPHNHTV 62 Query: 86 SQWFEHQERKLHG 98 QW + Q K +G Sbjct: 63 KQWMKVQAAKKNG 75 >1HA6-A nrPDB Length = 70 Score = 31.1 bits (69), Expect = 0.098, Method: Composition-based stats. Identities = 14/56 (25%), Positives = 25/56 (44%), Gaps = 4/56 (7%) Query: 33 CCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQR-SICIHPQNPSLSQ 87 CC + PL + ++ Q AD C + A + H +R S+C P+ + + Sbjct: 6 CCLSYIQTPLPSR---AIVGFTRQMADEACDINAIIFHTKKRKSVCADPKQNWVKR 58 >5LTL-A nrPDB Length = 100 Score = 30.4 bits (67), Expect = 0.20, Method: Composition-based stats. Identities = 13/61 (21%), Positives = 27/61 (44%), Gaps = 7/61 (11%) Query: 31 TACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQ-RSICIHPQNPSLSQWF 89 + CC + Y K L +L+ +CHL A + + R +C +P + + ++ Sbjct: 15 STCCLKYYEKVLPRRLVVGY------RKALNCHLPAIIFVTKRNREVCTNPNDDWVQEYI 68 Query: 90 E 90 + Sbjct: 69 K 69 >5CBA-E nrPDB Length = 88 Score = 28.8 bits (63), Expect = 0.56, Method: Composition-based stats. Identities = 14/69 (20%), Positives = 29/69 (42%), Gaps = 4/69 (5%) Query: 34 CTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSI-CIHPQNPSLSQWFEHQ 92 C Q + + + ++ L +G C + ++ +SI C+ PQ + + E Sbjct: 14 CVQESSVFIPRRFIDRIQI--LPRGNG-CPRKEIIVWKKNKSIVCVDPQAEWIQRMMEVL 70 Query: 93 ERKLHGTLP 101 ++ TLP Sbjct: 71 RKRSSSTLP 79 >1G2S-A nrPDB Length = 71 Score = 28.4 bits (62), Expect = 0.73, Method: Composition-based stats. Identities = 13/62 (20%), Positives = 24/62 (38%), Gaps = 6/62 (9%) Query: 29 PSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQ-RSICIHPQNPSLSQ 87 S CC Q KPL +R + C +A + + + +C HP+ + + Sbjct: 6 ISKTCCFQYSHKPLPWTWVR-----SYEFTSNSCSQRAVIFTTKRGKKVCTHPRKKWVQK 60 Query: 88 WF 89 + Sbjct: 61 YI 62 >2RA4-A nrPDB Length = 76 Score = 28.1 bits (61), Expect = 1.0, Method: Composition-based stats. Identities = 15/72 (20%), Positives = 28/72 (38%), Gaps = 6/72 (8%) Query: 27 LPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLA-QRSICIHPQNPSL 85 L + CC K +S + L+ + + C +A + + IC P+ + Sbjct: 6 LNVPSTCCFTFSSKKISLQRLKSYVITTSR-----CPQKAVIFRTKLGKEICADPKEKWV 60 Query: 86 SQWFEHQERKLH 97 + +H RK H Sbjct: 61 QNYMKHLGRKAH 72 >5A9Q-2 nrPDB Length = 326 Score = 26.9 bits (58), Expect = 1.8, Method: Composition-based stats. Identities = 12/48 (25%), Positives = 21/48 (43%), Gaps = 6/48 (12%) Query: 53 VELQEADGDCHLQAFVLHLAQRSICIHPQNPSLSQWFEHQERKLHGTL 100 + +G FVLH S+C HP+ + F+ + +GT+ Sbjct: 149 CRIWNLEG-VQTAHFVLHSPGMSVCWHPE-----ETFKLMVAEKNGTI 190 >2JYO-A nrPDB Length = 70 Score = 26.5 bits (57), Expect = 3.0, Method: Composition-based stats. Identities = 9/41 (21%), Positives = 19/41 (46%), Gaps = 1/41 (2%) Query: 56 QEADGDCHLQAFVLHLAQR-SICIHPQNPSLSQWFEHQERK 95 Q A+ C + A + H ++ S+C +P+ + +K Sbjct: 26 QLANEGCDINAIIFHTKKKLSVCANPKQTWVKYIVRLLSKK 66 >1NCV-A nrPDB Length = 76 Score = 25.4 bits (54), Expect = 5.6, Method: Composition-based stats. Identities = 16/75 (21%), Positives = 28/75 (37%), Gaps = 7/75 (9%) Query: 30 STACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLA-QRSICIHPQNPSLSQW 88 ST CC + K + + L + C +A + + IC P + + Sbjct: 8 STTCCYRFINKKIPKQRLESYR----RTTSSHCPREAVIFKTKLDKEICADPTQKWVQDF 63 Query: 89 FEHQERKLHGTLPKL 103 +H ++K PKL Sbjct: 64 MKHLDKKTQ--TPKL 76 >4L4I-A nrPDB Length = 547 Score = 25.4 bits (54), Expect = 6.6, Method: Composition-based stats. Identities = 8/39 (20%), Positives = 24/39 (61%) Query: 27 LPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQ 65 +PP + CT + + LS + L++++ +++++G ++ Sbjct: 58 VPPDLSICTFVLEQSLSVRALQEMLANTVEKSEGQVDVE 96 >3IM6-A nrPDB Length = 217 Score = 25.4 bits (54), Expect = 6.6, Method: Composition-based stats. Identities = 8/39 (20%), Positives = 24/39 (61%) Query: 27 LPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQ 65 +PP + CT + + LS + L++++ +++++G ++ Sbjct: 58 VPPDLSICTFVLEQSLSVRALQEMLANTVEKSEGQVDVE 96 >2Q0T-A nrPDB Length = 263 Score = 25.0 bits (53), Expect = 7.0, Method: Composition-based stats. Identities = 11/29 (37%), Positives = 16/29 (55%) Query: 20 DPTAAFLLPPSTACCTQLYRKPLSDKLLR 48 D T AF+ + AC TQLY ++ + R Sbjct: 58 DKTRAFIALAADACATQLYAPGVARHIER 86 >1NR4-A nrPDB Length = 71 Score = 25.0 bits (53), Expect = 7.0, Method: Composition-based stats. Identities = 13/60 (21%), Positives = 25/60 (41%), Gaps = 6/60 (10%) Query: 33 CCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFV-LHLAQRSICIHPQNPSLSQWFEH 91 CC + ++ + + L+ Q DC A V + + R+IC P N + ++ Sbjct: 10 CCLEYFKGAIPLRKLKTWYQ-----TSEDCSRDAIVFVTVQGRAICSDPNNKRVKNAVKY 64 >4X2Q-A nrPDB Length = 504 Score = 25.0 bits (53), Expect = 7.9, Method: Composition-based stats. Identities = 11/75 (14%), Positives = 30/75 (40%), Gaps = 14/75 (18%) Query: 33 CCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSICIHPQNPSLSQWFEHQ 92 CCT R + + + + ++ ++ A +R + P +P+ Q + Sbjct: 305 CCTAGSRIFVEESIYEEFVRRSVERA--------------KRRVVGSPFDPTTEQGPQID 350 Query: 93 ERKLHGTLPKLNFGM 107 +++ + L + G+ Sbjct: 351 KKQYNKILELIQSGV 365 >5BPZ-A nrPDB Length = 169 Score = 25.0 bits (53), Expect = 8.2, Method: Composition-based stats. Identities = 11/37 (29%), Positives = 17/37 (45%), Gaps = 1/37 (2%) Query: 12 LLSLLLSPDPTAAFLLPPSTACCTQLYRK-PLSDKLL 47 +L LL PD T + L+ CC + + KL+ Sbjct: 62 ILPLLQGPDMTLSKLIKIVEECCPNVSSSVHIRIKLM 98 >3FSR-A nrPDB Length = 352 Score = 24.6 bits (52), Expect = 9.7, Method: Composition-based stats. Identities = 10/44 (22%), Positives = 17/44 (38%) Query: 18 SPDPTAAFLLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGD 61 +P P A + P + A CT + + + EA G+ Sbjct: 21 APGPFDAIVRPLAVAPCTSDIHTVFEGAIGERHNMILGHEAVGE 64 >3FPC-A nrPDB Length = 352 Score = 24.6 bits (52), Expect = 9.7, Method: Composition-based stats. Identities = 10/44 (22%), Positives = 17/44 (38%) Query: 18 SPDPTAAFLLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGD 61 +P P A + P + A CT + + + EA G+ Sbjct: 21 APGPFDAIVRPLAVAPCTSDIHTVFEGAIGERHNMILGHEAVGE 64 >1YKF-A nrPDB Length = 352 Score = 24.6 bits (52), Expect = 9.7, Method: Composition-based stats. Identities = 10/44 (22%), Positives = 17/44 (38%) Query: 18 SPDPTAAFLLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGD 61 +P P A + P + A CT + + + EA G+ Sbjct: 21 APGPFDAIVRPLAVAPCTSDIHTVFEGAIGERHNMILGHEAVGE 64 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.320 0.139 0.423 Lambda K H 0.267 0.0419 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,752,250 Number of Sequences: 36641 Number of extensions: 160530 Number of successful extensions: 417 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 11 Number of HSP's that attempted gapping in prelim test: 402 Number of HSP's gapped (non-prelim): 25 length of query: 112 length of database: 10,336,785 effective HSP length: 78 effective length of query: 34 effective length of database: 7,478,787 effective search space: 254278758 effective search space used: 254278758 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.3 bits) S2: 52 (24.6 bits)