BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9Y4U1 (282 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3SOM-A nrPDB 576 e-165 5UJC-A nrPDB 144 3e-35 3TJT-A nrPDB 29 2.8 >3SOM-A nrPDB Length = 286 Score = 576 bits (1484), Expect = e-165, Method: Compositional matrix adjust. Identities = 282/282 (100%), Positives = 282/282 (100%) Query: 1 MEPKVAELKQKIEDTLCPFGFEVYPFQVAWYNELLPPAFHLPLPGPTLAFLVLSTPAMFD 60 MEPKVAELKQKIEDTLCPFGFEVYPFQVAWYNELLPPAFHLPLPGPTLAFLVLSTPAMFD Sbjct: 5 MEPKVAELKQKIEDTLCPFGFEVYPFQVAWYNELLPPAFHLPLPGPTLAFLVLSTPAMFD 64 Query: 61 RALKPFLQSCHLRMLTDPVDQCVAYHLGRVRESLPELQIEIIADYEVHPNRRPKILAQTA 120 RALKPFLQSCHLRMLTDPVDQCVAYHLGRVRESLPELQIEIIADYEVHPNRRPKILAQTA Sbjct: 65 RALKPFLQSCHLRMLTDPVDQCVAYHLGRVRESLPELQIEIIADYEVHPNRRPKILAQTA 124 Query: 121 AHVAGAAYYYQRQDVEADPWGNQRISGVCIHPRFGGWFAIRGVVLLPGIEVPDLPPRKPH 180 AHVAGAAYYYQRQDVEADPWGNQRISGVCIHPRFGGWFAIRGVVLLPGIEVPDLPPRKPH Sbjct: 125 AHVAGAAYYYQRQDVEADPWGNQRISGVCIHPRFGGWFAIRGVVLLPGIEVPDLPPRKPH 184 Query: 181 DCVPTRADRIALLEGFNFHWRDWTYRDAVTPQERYSEEQKAYFSTPPAQRLALLGLAQPS 240 DCVPTRADRIALLEGFNFHWRDWTYRDAVTPQERYSEEQKAYFSTPPAQRLALLGLAQPS Sbjct: 185 DCVPTRADRIALLEGFNFHWRDWTYRDAVTPQERYSEEQKAYFSTPPAQRLALLGLAQPS 244 Query: 241 EKPSSPSPDLPFTTPAPKKPGNPSRARSWLSPRVSPPASPGP 282 EKPSSPSPDLPFTTPAPKKPGNPSRARSWLSPRVSPPASPGP Sbjct: 245 EKPSSPSPDLPFTTPAPKKPGNPSRARSWLSPRVSPPASPGP 286 >5UJC-A nrPDB Length = 270 Score = 144 bits (364), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 85/239 (35%), Positives = 126/239 (52%), Gaps = 26/239 (10%) Query: 20 GFEVYPFQVAWYNELLPPA--FHLPLPGPTLAFLVLSTPAMFDRALK-----------PF 66 GFE + F++ YNE + + F LP T+A L+LSTP MFD A + F Sbjct: 24 GFESHMFKIGSYNEAVGESSPFALPYDDSTMALLILSTPDMFDVAFRKWVVQKTMDFGSF 83 Query: 67 LQSCHLRMLTDPVDQCVAYHLGRVRESLPELQ--IEIIADYEVHPNRRPKILAQTAAHVA 124 + C M++ P+ + L + E L +++ EI+ DY + P RRPKIL QT HVA Sbjct: 84 DEVC--EMVSSPIQSFLEDRLEIMSEKLRKVEENFEILHDYSMTPQRRPKILMQTCGHVA 141 Query: 125 GAAYYYQRQDVEAD-----PWG----NQRISGVCIHPRFGGWFAIRGVVLLPGIEVPDLP 175 GAA+YYQ + D P G N + G+ +HP +GG FA R V++ P +++P+ Sbjct: 142 GAAFYYQPCHFQEDGVTWPPAGRMGPNLKFIGLSLHPIYGGHFAFRSVLIFPNVKIPEFC 201 Query: 176 PRKPHDCVPTRADRIALLEGFNFHWRDWTYRDAVTPQERYSEEQKAYFSTPPAQRLALL 234 ++P + D LE FN++W+D +RD P RYS Q +F P A+R +L Sbjct: 202 EKEPRPILTASEDVRTALEKFNYNWKDSGFRDFGNPTRRYSTTQMEFFGRPVAERWEVL 260 >3TJT-A nrPDB Length = 208 Score = 28.9 bits (63), Expect = 2.8, Method: Compositional matrix adjust. Identities = 22/79 (27%), Positives = 35/79 (44%), Gaps = 1/79 (1%) Query: 50 FLVLSTPAMFDRALKPFLQSCHLRMLTDPVDQCVAYHLGRVRESLPELQIEIIADYEVHP 109 F V P +D AL+P++ +++ D Q L E PEL + + + Sbjct: 6 FKVKPLPYAYD-ALEPYIDKETMKLHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNL 64 Query: 110 NRRPKILAQTAAHVAGAAY 128 + PK +A T + AG AY Sbjct: 65 DSLPKDIATTVRNNAGGAY 83 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3SOM-A nrPDB 484 e-137 5UJC-A nrPDB 348 1e-96 Sequences not found previously or not previously below threshold: 4G2P-A nrPDB 32 0.38 2WVR-C nrPDB 30 1.0 4V6U-BE nrPDB 30 1.6 1M5Y-A nrPDB 29 2.0 1YW4-A nrPDB 29 2.6 4Q35-A nrPDB 28 5.8 4UUD-B nrPDB 28 6.1 1SOX-A nrPDB 28 6.7 CONVERGED! >3SOM-A nrPDB Length = 286 Score = 484 bits (1247), Expect = e-137, Method: Composition-based stats. Identities = 282/282 (100%), Positives = 282/282 (100%) Query: 1 MEPKVAELKQKIEDTLCPFGFEVYPFQVAWYNELLPPAFHLPLPGPTLAFLVLSTPAMFD 60 MEPKVAELKQKIEDTLCPFGFEVYPFQVAWYNELLPPAFHLPLPGPTLAFLVLSTPAMFD Sbjct: 5 MEPKVAELKQKIEDTLCPFGFEVYPFQVAWYNELLPPAFHLPLPGPTLAFLVLSTPAMFD 64 Query: 61 RALKPFLQSCHLRMLTDPVDQCVAYHLGRVRESLPELQIEIIADYEVHPNRRPKILAQTA 120 RALKPFLQSCHLRMLTDPVDQCVAYHLGRVRESLPELQIEIIADYEVHPNRRPKILAQTA Sbjct: 65 RALKPFLQSCHLRMLTDPVDQCVAYHLGRVRESLPELQIEIIADYEVHPNRRPKILAQTA 124 Query: 121 AHVAGAAYYYQRQDVEADPWGNQRISGVCIHPRFGGWFAIRGVVLLPGIEVPDLPPRKPH 180 AHVAGAAYYYQRQDVEADPWGNQRISGVCIHPRFGGWFAIRGVVLLPGIEVPDLPPRKPH Sbjct: 125 AHVAGAAYYYQRQDVEADPWGNQRISGVCIHPRFGGWFAIRGVVLLPGIEVPDLPPRKPH 184 Query: 181 DCVPTRADRIALLEGFNFHWRDWTYRDAVTPQERYSEEQKAYFSTPPAQRLALLGLAQPS 240 DCVPTRADRIALLEGFNFHWRDWTYRDAVTPQERYSEEQKAYFSTPPAQRLALLGLAQPS Sbjct: 185 DCVPTRADRIALLEGFNFHWRDWTYRDAVTPQERYSEEQKAYFSTPPAQRLALLGLAQPS 244 Query: 241 EKPSSPSPDLPFTTPAPKKPGNPSRARSWLSPRVSPPASPGP 282 EKPSSPSPDLPFTTPAPKKPGNPSRARSWLSPRVSPPASPGP Sbjct: 245 EKPSSPSPDLPFTTPAPKKPGNPSRARSWLSPRVSPPASPGP 286 >5UJC-A nrPDB Length = 270 Score = 348 bits (894), Expect = 1e-96, Method: Composition-based stats. Identities = 85/239 (35%), Positives = 126/239 (52%), Gaps = 26/239 (10%) Query: 20 GFEVYPFQVAWYNELLPPA--FHLPLPGPTLAFLVLSTPAMFDRALK-----------PF 66 GFE + F++ YNE + + F LP T+A L+LSTP MFD A + F Sbjct: 24 GFESHMFKIGSYNEAVGESSPFALPYDDSTMALLILSTPDMFDVAFRKWVVQKTMDFGSF 83 Query: 67 LQSCHLRMLTDPVDQCVAYHLGRVRESLPELQ--IEIIADYEVHPNRRPKILAQTAAHVA 124 + C M++ P+ + L + E L +++ EI+ DY + P RRPKIL QT HVA Sbjct: 84 DEVC--EMVSSPIQSFLEDRLEIMSEKLRKVEENFEILHDYSMTPQRRPKILMQTCGHVA 141 Query: 125 GAAYYYQRQDVEAD-----PWG----NQRISGVCIHPRFGGWFAIRGVVLLPGIEVPDLP 175 GAA+YYQ + D P G N + G+ +HP +GG FA R V++ P +++P+ Sbjct: 142 GAAFYYQPCHFQEDGVTWPPAGRMGPNLKFIGLSLHPIYGGHFAFRSVLIFPNVKIPEFC 201 Query: 176 PRKPHDCVPTRADRIALLEGFNFHWRDWTYRDAVTPQERYSEEQKAYFSTPPAQRLALL 234 ++P + D LE FN++W+D +RD P RYS Q +F P A+R +L Sbjct: 202 EKEPRPILTASEDVRTALEKFNYNWKDSGFRDFGNPTRRYSTTQMEFFGRPVAERWEVL 260 >4G2P-A nrPDB Length = 110 Score = 31.5 bits (70), Expect = 0.38, Method: Composition-based stats. Identities = 11/34 (32%), Positives = 17/34 (50%) Query: 54 STPAMFDRALKPFLQSCHLRMLTDPVDQCVAYHL 87 +TP +FD A + L H ++ PV +HL Sbjct: 68 ATPDIFDPAFRDALTKLHKGQISAPVHSSFGWHL 101 >2WVR-C nrPDB Length = 546 Score = 30.4 bits (67), Expect = 1.0, Method: Composition-based stats. Identities = 16/41 (39%), Positives = 20/41 (48%), Gaps = 2/41 (4%) Query: 243 PSSPSPDLPFTTPAPKKPG--NPSRARSWLSPRVSPPASPG 281 P P L TP+P +P P+ A S R PPA+PG Sbjct: 18 PRIAPPKLACRTPSPARPALRAPASATSGSRKRARPPAAPG 58 >4V6U-BE nrPDB Length = 186 Score = 29.6 bits (65), Expect = 1.6, Method: Composition-based stats. Identities = 10/16 (62%), Positives = 14/16 (87%) Query: 100 EIIADYEVHPNRRPKI 115 +I+AD+E HP RRP+I Sbjct: 10 QILADWEAHPMRRPRI 25 >1M5Y-A nrPDB Length = 408 Score = 29.2 bits (64), Expect = 2.0, Method: Composition-based stats. Identities = 10/34 (29%), Positives = 17/34 (50%) Query: 54 STPAMFDRALKPFLQSCHLRMLTDPVDQCVAYHL 87 +TP +FD A + L + ++ PV +HL Sbjct: 324 ATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHL 357 >1YW4-A nrPDB Length = 341 Score = 28.8 bits (63), Expect = 2.6, Method: Composition-based stats. Identities = 29/118 (24%), Positives = 38/118 (32%), Gaps = 15/118 (12%) Query: 99 IEIIADYEVHPNRRPKILAQTAA--------HVAGAAYYYQRQDVEADPW----GNQRIS 146 + D H R Q A Y+ Q EAD + G R Sbjct: 163 YPFLHDGRTH-KREQLAWLQRCGIEAVLLHTQPANTFSYFTSQYCEADAFTLELGKARPF 221 Query: 147 GVCIHPRFGG-WFAIRGVVLLPGIEVPDLPPRKPHDCVPTRADRIALLEGFNFHWRDW 203 G RF G A+RG++ P VPDL K + D + E F + D Sbjct: 222 GQNDLSRFSGIDGALRGLLSNPQANVPDLDEDK-LPLFRAKYDLVKHSEAFKLNLADS 278 >4Q35-A nrPDB Length = 802 Score = 27.7 bits (60), Expect = 5.8, Method: Composition-based stats. Identities = 16/58 (27%), Positives = 25/58 (43%), Gaps = 13/58 (22%) Query: 187 ADRIALLEGFNFHWRDWTYRDAVTPQERYSEEQK---------AYFSTPPAQRLALLG 235 DR+ L N+H+ Y A P + YS ++ A S P A R +++G Sbjct: 648 EDRLVQL---NYHYASPEYIQATLP-KYYSTAEQYKNGISQVGAVASRPIADRWSIVG 701 >4UUD-B nrPDB Length = 864 Score = 27.7 bits (60), Expect = 6.1, Method: Composition-based stats. Identities = 18/47 (38%), Positives = 20/47 (42%), Gaps = 11/47 (23%) Query: 234 LGLAQPSEKPSSPSPDLPFTTPAPKKPGNPSRARSWLSPRVSPPASP 280 LG A P SPD PF P P+ P P+RA PP P Sbjct: 809 LGGAPPVPSRPGASPD-PFG-PPPQVPSRPNRA---------PPGVP 844 >1SOX-A nrPDB Length = 466 Score = 27.7 bits (60), Expect = 6.7, Method: Composition-based stats. Identities = 15/45 (33%), Positives = 19/45 (42%), Gaps = 2/45 (4%) Query: 234 LGLAQPSEKPSSPSPDLPFTTPAPKKPGNPSRARSWLSPRVSPPA 278 +G P E P++P PF P+ PG R S PPA Sbjct: 79 VGELSPDEAPAAPDAQDPFAGDPPRHPG--LRVNSQKPFNAEPPA 121 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.311 0.137 0.413 Lambda K H 0.267 0.0406 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,381,940 Number of Sequences: 36641 Number of extensions: 522364 Number of successful extensions: 1291 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 17 Number of HSP's that attempted gapping in prelim test: 1246 Number of HSP's gapped (non-prelim): 46 length of query: 282 length of database: 10,336,785 effective HSP length: 96 effective length of query: 186 effective length of database: 6,819,249 effective search space: 1268380314 effective search space used: 1268380314 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.2 bits) S2: 58 (27.0 bits)