BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9Y4P1 (393 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2Z0E-A nrPDB 739 0.0 2P82-A nrPDB 429 e-120 4Z9R-A nrPDB 30 1.2 2Y7I-A nrPDB 30 1.3 5EJJ-A nrPDB 29 3.3 >2Z0E-A nrPDB Length = 357 Score = 739 bits (1907), Expect = 0.0, Method: Compositional matrix adjust. Identities = 352/353 (99%), Positives = 352/353 (99%) Query: 1 MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPA 60 MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPA Sbjct: 4 MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPA 63 Query: 61 IGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKD 120 IGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKD Sbjct: 64 IGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKD 123 Query: 121 SYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIR 180 SYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIR Sbjct: 124 SYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIR 183 Query: 181 RLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYV 240 RLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYV Sbjct: 184 RLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYV 243 Query: 241 ETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFH 300 ETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDP TTQPAVEPTDGCFIPDESFH Sbjct: 244 ETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPATTQPAVEPTDGCFIPDESFH 303 Query: 301 CQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVE 353 CQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVE Sbjct: 304 CQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVE 356 >2P82-A nrPDB Length = 355 Score = 429 bits (1103), Expect = e-120, Method: Compositional matrix adjust. Identities = 198/342 (57%), Positives = 259/342 (75%), Gaps = 9/342 (2%) Query: 20 PETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPAIGGTGPTSDTGWGCMLRCG 79 P+T E VWILG+++ + TEK ++LSD+++RLWFTYR+ F IGGTGP+SD GWGCMLRCG Sbjct: 19 PDTDELVWILGKQHLLKTEKSKLLSDISARLWFTYRRKFSPIGGTGPSSDAGWGCMLRCG 78 Query: 80 QMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKDSYYSIHQIAQMGVGEGKSI 139 QM+ AQAL+CRHLGRDW W ++K QP Y +L F+DRKD YSIHQ+AQMGVGEGKSI Sbjct: 79 QMMLAQALICRHLGRDWSWEKQKEQPKEYQRILQCFLDRKDCCYSIHQMAQMGVGEGKSI 138 Query: 140 GQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIRRLCRTSVPCAGATAFPADS 199 G+W+GPNTVAQVLKKLA+FD W+SLAV+++MDNTVV+E+I+++CR +P + TA Sbjct: 139 GEWFGPNTVAQVLKKLALFDEWNSLAVYVSMDNTVVIEDIKKMCRV-LPLSADTA----G 193 Query: 200 DRHCNGFPAGAEVTNRP---SPWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVI 256 DR + A + S W+PL+L++PLRLG+ IN YV+ K CF MPQSLG + Sbjct: 194 DRPPDSLTASNQSKGTSAYCSAWKPLLLIVPLRLGINQINPVYVDAFKECFKMPQSLGAL 253 Query: 257 GGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFHCQHPPCRMSIAELDPS 316 GGKPN+A+YFIG++G+ELI+LDPHTTQ V+ + + D++FHC P RM+I LDPS Sbjct: 254 GGKPNNAYYFIGFLGDELIFLDPHTTQTFVDTEENGTVNDQTFHCLQSPQRMNILNLDPS 313 Query: 317 IAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVELQPSH 358 +A+GFFCK E DF++WC V+K +L L MFELV+ PSH Sbjct: 314 VALGFFCKEEKDFDNWCSLVQK-EILKENLRMFELVQKHPSH 354 >4Z9R-A nrPDB Length = 547 Score = 30.4 bits (67), Expect = 1.2, Method: Compositional matrix adjust. Identities = 26/87 (29%), Positives = 35/87 (40%), Gaps = 19/87 (21%) Query: 172 NTVVMEEIRRLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLG 231 N ++ E + L SVP A ADS H + RPLV L+P L Sbjct: 247 NEGLISEDQMLMAQSVPMADDITAEADSGGHTDN--------------RPLVTLLPTILA 292 Query: 232 LTDINEAYVETLKHCFMMPQSLGVIGG 258 L D +A K+ + P +G GG Sbjct: 293 LKDTIQA-----KYQYKTPIRVGAGGG 314 >2Y7I-A nrPDB Length = 229 Score = 30.4 bits (67), Expect = 1.3, Method: Compositional matrix adjust. Identities = 26/90 (28%), Positives = 43/90 (47%), Gaps = 13/90 (14%) Query: 97 RWTQRKRQ---PDSYFSVLNAFIDRKDSYYSIHQIAQMGV-GEGKSIGQWYGPNTVAQVL 152 R+ Q K+Q P +Y S LNAF D K++ GV G+ +IG+W N ++ Sbjct: 125 RYLQDKQQAITPVAYDSYLNAFTDLKNNRLE-------GVFGDVAAIGKWLKNNPDYAIM 177 Query: 153 KKLAVFDTW--SSLAVHIAMDNTVVMEEIR 180 + A + L + + DN +++EI Sbjct: 178 DERASDPDYYGKGLGIAVRKDNDALLQEIN 207 >5EJJ-A nrPDB Length = 564 Score = 29.3 bits (64), Expect = 3.3, Method: Compositional matrix adjust. Identities = 31/121 (25%), Positives = 48/121 (39%), Gaps = 34/121 (28%) Query: 69 DTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRK-DSYYSIHQ 127 D+GWGC R Q I W W +LN + D+ S+ I Q Sbjct: 391 DSGWGCAYRSFQTI-------------WSWF-----------ILNGYTDKPVPSHREIQQ 426 Query: 128 -IAQMGVGEGKSIG--QWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTV-VMEEIRRLC 183 + + + K +G QW G ++ VL +L + IA ++ V+E +R L Sbjct: 427 ALVDIQDKQAKFVGSRQWIGSTEISFVLNELLKLE-----CRFIATNSGAEVVERVRELA 481 Query: 184 R 184 R Sbjct: 482 R 482 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2Z0E-A nrPDB 720 0.0 2P82-A nrPDB 612 e-176 Sequences not found previously or not previously below threshold: 5EJJ-A nrPDB 32 0.36 4Z9R-A nrPDB 31 0.90 4LGC-A nrPDB 30 1.5 2Y7I-A nrPDB 30 2.0 3G04-C nrPDB 30 2.4 4KNH-A nrPDB 28 5.6 CONVERGED! >2Z0E-A nrPDB Length = 357 Score = 720 bits (1858), Expect = 0.0, Method: Composition-based stats. Identities = 352/354 (99%), Positives = 352/354 (99%) Query: 1 MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPA 60 MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPA Sbjct: 4 MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPA 63 Query: 61 IGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKD 120 IGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKD Sbjct: 64 IGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKD 123 Query: 121 SYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIR 180 SYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIR Sbjct: 124 SYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIR 183 Query: 181 RLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYV 240 RLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYV Sbjct: 184 RLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYV 243 Query: 241 ETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFH 300 ETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDP TTQPAVEPTDGCFIPDESFH Sbjct: 244 ETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPATTQPAVEPTDGCFIPDESFH 303 Query: 301 CQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVEL 354 CQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVE Sbjct: 304 CQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVEQ 357 >2P82-A nrPDB Length = 355 Score = 612 bits (1579), Expect = e-176, Method: Composition-based stats. Identities = 198/342 (57%), Positives = 259/342 (75%), Gaps = 9/342 (2%) Query: 20 PETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPAIGGTGPTSDTGWGCMLRCG 79 P+T E VWILG+++ + TEK ++LSD+++RLWFTYR+ F IGGTGP+SD GWGCMLRCG Sbjct: 19 PDTDELVWILGKQHLLKTEKSKLLSDISARLWFTYRRKFSPIGGTGPSSDAGWGCMLRCG 78 Query: 80 QMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKDSYYSIHQIAQMGVGEGKSI 139 QM+ AQAL+CRHLGRDW W ++K QP Y +L F+DRKD YSIHQ+AQMGVGEGKSI Sbjct: 79 QMMLAQALICRHLGRDWSWEKQKEQPKEYQRILQCFLDRKDCCYSIHQMAQMGVGEGKSI 138 Query: 140 GQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIRRLCRTSVPCAGATAFPADS 199 G+W+GPNTVAQVLKKLA+FD W+SLAV+++MDNTVV+E+I+++CR +P + TA Sbjct: 139 GEWFGPNTVAQVLKKLALFDEWNSLAVYVSMDNTVVIEDIKKMCRV-LPLSADTA----G 193 Query: 200 DRHCNGFPAGAEVTNRP---SPWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVI 256 DR + A + S W+PL+L++PLRLG+ IN YV+ K CF MPQSLG + Sbjct: 194 DRPPDSLTASNQSKGTSAYCSAWKPLLLIVPLRLGINQINPVYVDAFKECFKMPQSLGAL 253 Query: 257 GGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFHCQHPPCRMSIAELDPS 316 GGKPN+A+YFIG++G+ELI+LDPHTTQ V+ + + D++FHC P RM+I LDPS Sbjct: 254 GGKPNNAYYFIGFLGDELIFLDPHTTQTFVDTEENGTVNDQTFHCLQSPQRMNILNLDPS 313 Query: 317 IAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVELQPSH 358 +A+GFFCK E DF++WC V+K +L L MFELV+ PSH Sbjct: 314 VALGFFCKEEKDFDNWCSLVQK-EILKENLRMFELVQKHPSH 354 >5EJJ-A nrPDB Length = 564 Score = 32.3 bits (72), Expect = 0.36, Method: Composition-based stats. Identities = 43/193 (22%), Positives = 74/193 (38%), Gaps = 49/193 (25%) Query: 11 LRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPAIGG----TGP 66 +R + + P +S+PV L + ++ +E + + R N+ +G GP Sbjct: 320 IRLHKLFNLP-SSKPVLRLSQALALHSESARLTNKKLIREPHLSITNYQPVGEITTVNGP 378 Query: 67 TS----------DTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFI 116 + D+GWGC R Q I W W +LN + Sbjct: 379 YNYHHYMQDGIDDSGWGCAYRSFQTI-------------WSWF-----------ILNGYT 414 Query: 117 DRK-DSYYSIHQ-IAQMGVGEGKSIG--QWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDN 172 D+ S+ I Q + + + K +G QW G ++ VL +L + IA ++ Sbjct: 415 DKPVPSHREIQQALVDIQDKQAKFVGSRQWIGSTEISFVLNELLKLE-----CRFIATNS 469 Query: 173 TV-VMEEIRRLCR 184 V+E +R L R Sbjct: 470 GAEVVERVRELAR 482 >4Z9R-A nrPDB Length = 547 Score = 31.1 bits (69), Expect = 0.90, Method: Composition-based stats. Identities = 28/94 (29%), Positives = 39/94 (41%), Gaps = 21/94 (22%) Query: 172 NTVVMEEIRRLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLG 231 N ++ E + L SVP A ADS H + RPLV L+P L Sbjct: 247 NEGLISEDQMLMAQSVPMADDITAEADSGGHTDN--------------RPLVTLLPTILA 292 Query: 232 LTDINEAYVETLKHCFMMPQSLGVIG--GKPNSA 263 L D +A K+ + P +G G G P++A Sbjct: 293 LKDTIQA-----KYQYKTPIRVGAGGGIGTPDAA 321 >4LGC-A nrPDB Length = 539 Score = 30.3 bits (67), Expect = 1.5, Method: Composition-based stats. Identities = 18/71 (25%), Positives = 29/71 (40%), Gaps = 4/71 (5%) Query: 133 VGEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIRRLCRTSVPCAGA 192 +G G + + PN++ ++ AVF W + A ++ M EIR C T P A Sbjct: 101 IGPGSIVLTMF-PNSIEHII---AVFAIWKAGACYMPMSYKAAESEIREACDTIHPNAAF 156 Query: 193 TAFPADSDRHC 203 + C Sbjct: 157 AECKIPGLKFC 167 >2Y7I-A nrPDB Length = 229 Score = 30.0 bits (66), Expect = 2.0, Method: Composition-based stats. Identities = 26/89 (29%), Positives = 43/89 (48%), Gaps = 13/89 (14%) Query: 97 RWTQRKRQ---PDSYFSVLNAFIDRKDSYYSIHQIAQMGV-GEGKSIGQWYGPNTVAQVL 152 R+ Q K+Q P +Y S LNAF D K++ GV G+ +IG+W N ++ Sbjct: 125 RYLQDKQQAITPVAYDSYLNAFTDLKNNRLE-------GVFGDVAAIGKWLKNNPDYAIM 177 Query: 153 KKLAVFDTW--SSLAVHIAMDNTVVMEEI 179 + A + L + + DN +++EI Sbjct: 178 DERASDPDYYGKGLGIAVRKDNDALLQEI 206 >3G04-C nrPDB Length = 239 Score = 29.6 bits (65), Expect = 2.4, Method: Composition-based stats. Identities = 18/72 (25%), Positives = 30/72 (41%), Gaps = 20/72 (27%) Query: 301 CQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVELQ----P 356 C PPC C E+DF C+ ++++ L + +L+E P Sbjct: 3 CSSPPCE---------------CHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIP 47 Query: 357 SHLACPDVLNLS 368 SH A ++ N+S Sbjct: 48 SH-AFSNLPNIS 58 >4KNH-A nrPDB Length = 961 Score = 28.4 bits (62), Expect = 5.6, Method: Composition-based stats. Identities = 24/78 (30%), Positives = 39/78 (50%), Gaps = 12/78 (15%) Query: 321 FFCKTEDDFNDWCQQVKKLSLLGGALPMFELVELQPSHL--ACPDVLNLSLDSSDVERLE 378 F CK D+ + W L +LG A+ + + E +L D+ ++LD D +R + Sbjct: 312 FICKGGDE-DSWA-----LPVLGAAVRAWWIAEHNGFYLDDTVQDLRGINLDEEDEQRTK 365 Query: 379 RFFDSEDE---DFEILSL 393 +F D+ E DF ILS+ Sbjct: 366 QFLDALKEGAFDF-ILSV 382 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.141 0.441 Lambda K H 0.267 0.0430 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 17,650,235 Number of Sequences: 36641 Number of extensions: 771133 Number of successful extensions: 1503 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 1492 Number of HSP's gapped (non-prelim): 13 length of query: 393 length of database: 10,336,785 effective HSP length: 99 effective length of query: 294 effective length of database: 6,709,326 effective search space: 1972541844 effective search space used: 1972541844 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 60 (27.7 bits)