BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9Y3E7 (222 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3FRT-A nrPDB 425 e-120 2GD5-A nrPDB 347 2e-96 3FRV-A nrPDB 300 4e-82 3IBV-A nrPDB 32 0.16 6MSG-W nrPDB 30 0.79 5XGS-A nrPDB 28 3.6 3E6I-A nrPDB 27 4.8 5JP0-A nrPDB 27 6.1 2I91-A nrPDB 27 6.4 >3FRT-A nrPDB Length = 218 Score = 425 bits (1093), Expect = e-120, Method: Compositional matrix adjust. Identities = 216/218 (99%), Positives = 216/218 (99%) Query: 5 GKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLA 64 G QEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLA Sbjct: 1 GHMQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLA 60 Query: 65 KEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQ 124 KEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQ Sbjct: 61 KEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQ 120 Query: 125 ATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKV 184 ATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKV Sbjct: 121 ATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKV 180 Query: 185 TDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS 222 TDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS Sbjct: 181 TDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS 218 >2GD5-A nrPDB Length = 179 Score = 347 bits (891), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 176/179 (98%), Positives = 176/179 (98%) Query: 5 GKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLA 64 G EKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLA Sbjct: 1 GAMAEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLA 60 Query: 65 KEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQ 124 KEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQ Sbjct: 61 KEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQ 120 Query: 125 ATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSK 183 ATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSK Sbjct: 121 ATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSK 179 >3FRV-A nrPDB Length = 152 Score = 300 bits (767), Expect = 4e-82, Method: Compositional matrix adjust. Identities = 150/150 (100%), Positives = 150/150 (100%) Query: 1 MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVC 60 MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVC Sbjct: 3 MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVC 62 Query: 61 IVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKI 120 IVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKI Sbjct: 63 IVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKI 122 Query: 121 PEIQATMRELSKEMMKAGIIEEMLEDTFES 150 PEIQATMRELSKEMMKAGIIEEMLEDTFES Sbjct: 123 PEIQATMRELSKEMMKAGIIEEMLEDTFES 152 >3IBV-A nrPDB Length = 980 Score = 32.3 bits (72), Expect = 0.16, Method: Composition-based stats. Identities = 16/43 (37%), Positives = 28/43 (65%), Gaps = 1/43 (2%) Query: 122 EIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEME 164 E+ A+++E K +++A II++M D + DD + EEEAE + Sbjct: 371 ELSASLKEFLKSLLEA-IIKKMKYDESQEWDDDPDSEEEAEFQ 412 >6MSG-W nrPDB Length = 456 Score = 30.0 bits (66), Expect = 0.79, Method: Compositional matrix adjust. Identities = 13/40 (32%), Positives = 22/40 (55%) Query: 2 GLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREE 41 G+ + K P L+N+WS K+ M +V++ I +EE Sbjct: 411 GIINFQRPKDPNNLLNDWSQKLNSLMSLVNKTTHLIAKEE 450 >5XGS-A nrPDB Length = 427 Score = 27.7 bits (60), Expect = 3.6, Method: Compositional matrix adjust. Identities = 14/37 (37%), Positives = 18/37 (48%) Query: 169 LFEITAGALGKAPSKVTDALPEPEPPGAMAASEDEEE 205 +F G LGK P+ V A+PE PP +E E Sbjct: 320 VFVWGYGILGKGPNLVESAVPEMIPPTLFGLTEFNPE 356 >3E6I-A nrPDB Length = 476 Score = 27.3 bits (59), Expect = 4.8, Method: Compositional matrix adjust. Identities = 22/88 (25%), Positives = 39/88 (44%), Gaps = 8/88 (9%) Query: 42 EKVKRSVKDAAKKGQKDVCIVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVA 101 E+VK + +D+ L EM + + + +LY + G+ +A L A Sbjct: 227 ERVKEHHQSLDPNCPRDLTDCLLVEMEKEKHSAERLYT--------MDGITVTVADLFFA 278 Query: 102 GSLQKSTEVMKAMQSLVKIPEIQATMRE 129 G+ ST + + L+K PEI+ + E Sbjct: 279 GTETTSTTLRYGLLILMKYPEIEEKLHE 306 >5JP0-A nrPDB Length = 772 Score = 26.9 bits (58), Expect = 6.1, Method: Composition-based stats. Identities = 16/46 (34%), Positives = 25/46 (54%) Query: 123 IQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRI 168 I AT +E K ++ AGI M+ D +E+ EE E+ ++RI Sbjct: 305 IAATKKEAVKIVINAGIDMSMVPYEVSFCDYLKELVEEGEVSMERI 350 >2I91-A nrPDB Length = 538 Score = 26.9 bits (58), Expect = 6.4, Method: Compositional matrix adjust. Identities = 19/56 (33%), Positives = 27/56 (48%), Gaps = 1/56 (1%) Query: 99 RVAGSLQKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGI-IEEMLEDTFESMDD 153 RV GS+ ++ V AM LV E + M S EM+ I + +L + E M D Sbjct: 384 RVLGSILNASVVAAAMCMLVARTEKDSHMVAFSDEMLPCPITVNMLLHEVVEKMSD 439 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3FRT-A nrPDB 315 1e-86 2GD5-A nrPDB 261 2e-70 3FRV-A nrPDB 231 2e-61 Sequences not found previously or not previously below threshold: 3IBV-A nrPDB 34 0.040 6E8G-T nrPDB 31 0.43 3JC1-Ab nrPDB 30 0.55 3E6I-A nrPDB 30 0.87 5JP0-A nrPDB 29 2.5 5XGS-A nrPDB 28 2.9 6MSG-W nrPDB 28 3.5 1G6Q-1 nrPDB 28 3.8 6EM8-A nrPDB 28 4.4 2I91-A nrPDB 27 4.7 CONVERGED! >3FRT-A nrPDB Length = 218 Score = 315 bits (806), Expect = 1e-86, Method: Composition-based stats. Identities = 216/218 (99%), Positives = 216/218 (99%) Query: 5 GKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLA 64 G QEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLA Sbjct: 1 GHMQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLA 60 Query: 65 KEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQ 124 KEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQ Sbjct: 61 KEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQ 120 Query: 125 ATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKV 184 ATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKV Sbjct: 121 ATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKV 180 Query: 185 TDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS 222 TDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS Sbjct: 181 TDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS 218 >2GD5-A nrPDB Length = 179 Score = 261 bits (667), Expect = 2e-70, Method: Composition-based stats. Identities = 176/179 (98%), Positives = 176/179 (98%) Query: 5 GKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLA 64 G EKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLA Sbjct: 1 GAMAEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLA 60 Query: 65 KEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQ 124 KEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQ Sbjct: 61 KEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQ 120 Query: 125 ATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSK 183 ATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSK Sbjct: 121 ATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSK 179 >3FRV-A nrPDB Length = 152 Score = 231 bits (589), Expect = 2e-61, Method: Composition-based stats. Identities = 150/150 (100%), Positives = 150/150 (100%) Query: 1 MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVC 60 MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVC Sbjct: 3 MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVC 62 Query: 61 IVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKI 120 IVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKI Sbjct: 63 IVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKI 122 Query: 121 PEIQATMRELSKEMMKAGIIEEMLEDTFES 150 PEIQATMRELSKEMMKAGIIEEMLEDTFES Sbjct: 123 PEIQATMRELSKEMMKAGIIEEMLEDTFES 152 >3IBV-A nrPDB Length = 980 Score = 34.3 bits (77), Expect = 0.040, Method: Composition-based stats. Identities = 16/43 (37%), Positives = 28/43 (65%), Gaps = 1/43 (2%) Query: 122 EIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEME 164 E+ A+++E K +++A II++M D + DD + EEEAE + Sbjct: 371 ELSASLKEFLKSLLEA-IIKKMKYDESQEWDDDPDSEEEAEFQ 412 >6E8G-T nrPDB Length = 199 Score = 30.8 bits (68), Expect = 0.43, Method: Composition-based stats. Identities = 25/134 (18%), Positives = 57/134 (42%) Query: 30 VDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLAKEMIRSRKAVSKLYASKAHMNSVLM 89 + R + +EE+ K ++ A +KG +V + A+ IR + A +++V Sbjct: 18 LSRSAKKCDKEEKAEKAKIEKAIQKGNMEVARIHAENAIRQKNQAVNFLRMSARVDAVAA 77 Query: 90 GMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFE 149 ++ + + +V S+ + M A + + +I A M + + + + +EDT Sbjct: 78 RVQTAVTMGKVTKSMAGVVKSMDATLKTMNLEKISALMDKFEHQFETLDVQTQQMEDTMS 137 Query: 150 SMDDQEEMEEEAEM 163 S + + +M Sbjct: 138 STTTLTTPQNQVDM 151 >3JC1-Ab nrPDB Length = 160 Score = 30.4 bits (67), Expect = 0.55, Method: Composition-based stats. Identities = 25/134 (18%), Positives = 57/134 (42%) Query: 30 VDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLAKEMIRSRKAVSKLYASKAHMNSVLM 89 + R + +EE+ K ++ A +KG +V + A+ IR + A +++V Sbjct: 15 LSRSAKKCDKEEKAEKAKIEKAIQKGNMEVARIHAENAIRQKNQAVNFLRMSARVDAVAA 74 Query: 90 GMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFE 149 ++ + + +V S+ + M A + + +I A M + + + + +EDT Sbjct: 75 RVQTAVTMGKVTKSMAGVVKSMDATLKTMNLEKISALMDKFEHQFETLDVQTQQMEDTMS 134 Query: 150 SMDDQEEMEEEAEM 163 S + + +M Sbjct: 135 STTTLTTPQNQVDM 148 >3E6I-A nrPDB Length = 476 Score = 30.0 bits (66), Expect = 0.87, Method: Composition-based stats. Identities = 22/88 (25%), Positives = 39/88 (44%), Gaps = 8/88 (9%) Query: 42 EKVKRSVKDAAKKGQKDVCIVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVA 101 E+VK + +D+ L EM + + + +LY + G+ +A L A Sbjct: 227 ERVKEHHQSLDPNCPRDLTDCLLVEMEKEKHSAERLYT--------MDGITVTVADLFFA 278 Query: 102 GSLQKSTEVMKAMQSLVKIPEIQATMRE 129 G+ ST + + L+K PEI+ + E Sbjct: 279 GTETTSTTLRYGLLILMKYPEIEEKLHE 306 >5JP0-A nrPDB Length = 772 Score = 28.5 bits (62), Expect = 2.5, Method: Composition-based stats. Identities = 16/46 (34%), Positives = 25/46 (54%) Query: 123 IQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRI 168 I AT +E K ++ AGI M+ D +E+ EE E+ ++RI Sbjct: 305 IAATKKEAVKIVINAGIDMSMVPYEVSFCDYLKELVEEGEVSMERI 350 >5XGS-A nrPDB Length = 427 Score = 28.1 bits (61), Expect = 2.9, Method: Composition-based stats. Identities = 13/31 (41%), Positives = 16/31 (51%) Query: 175 GALGKAPSKVTDALPEPEPPGAMAASEDEEE 205 G LGK P+ V A+PE PP +E E Sbjct: 326 GILGKGPNLVESAVPEMIPPTLFGLTEFNPE 356 >6MSG-W nrPDB Length = 456 Score = 27.7 bits (60), Expect = 3.5, Method: Composition-based stats. Identities = 13/40 (32%), Positives = 22/40 (55%) Query: 2 GLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREE 41 G+ + K P L+N+WS K+ M +V++ I +EE Sbjct: 411 GIINFQRPKDPNNLLNDWSQKLNSLMSLVNKTTHLIAKEE 450 >1G6Q-1 nrPDB Length = 328 Score = 27.7 bits (60), Expect = 3.8, Method: Composition-based stats. Identities = 11/38 (28%), Positives = 23/38 (60%) Query: 138 GIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAG 175 GI EEML+DT ++ + + + ++ D+I+ ++ G Sbjct: 11 GIHEEMLQDTVRTLSYRNAIIQNKDLFKDKIVLDVGCG 48 >6EM8-A nrPDB Length = 818 Score = 27.7 bits (60), Expect = 4.4, Method: Composition-based stats. Identities = 16/44 (36%), Positives = 27/44 (61%), Gaps = 1/44 (2%) Query: 14 ELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQK 57 +L++E S K+R + +++I++E EKVK KDAA Q+ Sbjct: 393 DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNE-KDAAVHAQE 435 >2I91-A nrPDB Length = 538 Score = 27.3 bits (59), Expect = 4.7, Method: Composition-based stats. Identities = 19/56 (33%), Positives = 27/56 (48%), Gaps = 1/56 (1%) Query: 99 RVAGSLQKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGI-IEEMLEDTFESMDD 153 RV GS+ ++ V AM LV E + M S EM+ I + +L + E M D Sbjct: 384 RVLGSILNASVVAAAMCMLVARTEKDSHMVAFSDEMLPCPITVNMLLHEVVEKMSD 439 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.309 0.133 0.365 Lambda K H 0.267 0.0389 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,094,482 Number of Sequences: 36641 Number of extensions: 249362 Number of successful extensions: 1275 Number of sequences better than 10.0: 42 Number of HSP's better than 10.0 without gapping: 27 Number of HSP's successfully gapped in prelim test: 66 Number of HSP's that attempted gapping in prelim test: 1224 Number of HSP's gapped (non-prelim): 107 length of query: 222 length of database: 10,336,785 effective HSP length: 93 effective length of query: 129 effective length of database: 6,929,172 effective search space: 893863188 effective search space used: 893863188 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 57 (26.6 bits)