BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9X1G6 (183 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5KBW-A nrPDB 330 3e-91 3P5N-A nrPDB 81 2e-16 4Z7F-A nrPDB 33 0.099 3ZGJ-A nrPDB 29 1.1 3V7P-A nrPDB 27 3.6 4X8F-A nrPDB 27 4.4 >5KBW-A nrPDB Length = 181 Score = 330 bits (845), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 172/174 (98%), Positives = 172/174 (98%) Query: 2 SSIKKISFVGIFSALATLVMFLEFPIFPQASFLKYDPSEIPALIVSFLLGPGVGMFVVLV 61 SSIKKISFVGIFSALATLVMFLEFPIFPQASFLKYDPSEIPALIVSFLLGPGVGMFVVLV Sbjct: 3 SSIKKISFVGIFSALATLVMFLEFPIFPQASFLKYDPSEIPALIVSFLLGPGVGMFVVLV 62 Query: 62 KDILFFLMKSGDPVGIAMNAVLGMSFVGIAGLIYHRNKSRATAIKGMIVATLFATAFALG 121 KDILFFLMKSGDPVGIAMNAVLGMSFVGIAGLIYHRNKSRATAIKGMIVATLFATAFALG Sbjct: 63 KDILFFLMKSGDPVGIAMNAVLGMSFVGIAGLIYHRNKSRATAIKGMIVATLFATAFALG 122 Query: 122 LNALIVPLYFEAPFELYLKFFPFILAFNLVKFGIDSVVTFFVYKKVSSILKLET 175 LNALIVPLYFEAPFELYLKFFPFILAFNLVKFGIDSVVTFFVYKKVSSILKL Sbjct: 123 LNALIVPLYFEAPFELYLKFFPFILAFNLVKFGIDSVVTFFVYKKVSSILKLSN 176 >3P5N-A nrPDB Length = 189 Score = 81.3 bits (199), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 58/179 (32%), Positives = 100/179 (55%), Gaps = 7/179 (3%) Query: 1 MSSIKKISFVGIFSALATLVMFLEFPIFPQASFLKYDPSEIPALIVSFLLGPGVGMFVVL 60 M K++ + + SA+A ++ F++FPI +L D S++P+L+ +F GP G+ V L Sbjct: 9 MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSLLATFTFGPVAGIIVAL 68 Query: 61 VKDILFFLMKSGDPVGIAMNAVLGMSFVGIAGLIYHRNKSRATAIKGMIVATLFATAFAL 120 VK++L +L GDPVG N + G SF+ A IY +S + I G+I+AT+ T Sbjct: 69 VKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTKSLITGLIIATIVMTIVLS 128 Query: 121 GLNA-LIVPLY------FEAPFELYLKFFPFILAFNLVKFGIDSVVTFFVYKKVSSILK 172 LN +++PLY + L + I+ FN++K + S+V +Y+++++ LK Sbjct: 129 ILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKGIVISIVFILLYRRLANFLK 187 >4Z7F-A nrPDB Length = 199 Score = 32.7 bits (73), Expect = 0.099, Method: Compositional matrix adjust. Identities = 45/170 (26%), Positives = 83/170 (48%), Gaps = 21/170 (12%) Query: 5 KKISFVGIFSALATLVMFLEFPIFPQASFLKYDPSEIPALIVSFLLGP---GVGMFVVLV 61 K I+ +G+ +A +V+F F + + +FLK + IP ++ + GP G+G V V Sbjct: 29 KSIALMGVL--IAVVVVFSRFFAY-ETTFLKISFTFIPESLIGMIFGPFWAGIGTAVADV 85 Query: 62 KDILFFLMKSGDPVGIAMNAVLGMSFVGIAGLIYHRNKSRATAIKGMIVATLFATAFALG 121 +L F K+G G +NA L + I G Y++ + + +I+ATL T Sbjct: 86 VGMLLF-PKAGYFPGFTLNAFLAGA---IYGYFYYK---KEMTWQRVILATLLVTVL--- 135 Query: 122 LNALIVPLYFEAPFELYLKFFPFILAFNLVK----FGIDSVVTFFVYKKV 167 +N ++ PL+ + + L F + + L+K F I + T+++ K+ Sbjct: 136 INIILTPLWLSLMYGVNLANFAWWVP-RLIKTVIFFPIQVIATYYLGNKI 184 >3ZGJ-A nrPDB Length = 371 Score = 29.3 bits (64), Expect = 1.1, Method: Compositional matrix adjust. Identities = 33/155 (21%), Positives = 60/155 (38%), Gaps = 24/155 (15%) Query: 32 SFLKYDPSEIPALIVSFLL---GPGVGMFVVLVKDILFFLMKSGDPVGIAMNAVLGMSFV 88 + ++ D + +P I FL GPGV LV DI+ + GD G++F+ Sbjct: 234 TLIEPDDTRVPGQIDQFLSAHDGPGVQHLAFLVDDIVGSVRSLGD---------RGVAFL 284 Query: 89 GIAGLIY----HRNKSRATAIKGMIVATLFATAFALGLNALIVPLYFEAPFELYLKFFPF 144 G Y R + A AI+ + + A G ++ ++ +P+ F+ + Sbjct: 285 RTPGAYYDLLTERVGAMADAIEDLRETNVLADRDEWG---YLLQIFTRSPYPRGTLFYEY 341 Query: 145 ILAFNLVKFGIDSVVTFFVYKKVSSILKLETVEGR 179 I FG ++ ++ + V GR Sbjct: 342 IQRNGARGFGSSNIKAL-----AEAVEREREVAGR 371 >3V7P-A nrPDB Length = 427 Score = 27.3 bits (59), Expect = 3.6, Method: Compositional matrix adjust. Identities = 32/115 (27%), Positives = 50/115 (43%), Gaps = 6/115 (5%) Query: 8 SFVGIFSALATLVMFLEFPIFPQ-ASFLKYDPSEIPALIVSFLLGPGVGMFVVLVKDILF 66 SF+ +F L TL + + + + + Y+ I I + LLG GV + + +K I + Sbjct: 243 SFLELFKELHTLFVHMVWANEEEIQTIASYNAHIIHCPISNRLLGNGV-LDLEKIKSIPY 301 Query: 67 FLMKSGDPVGIAMNAVLGMSFVGIAGLIYHRNKSRATAIKGMIVATLFATAFALG 121 + G ++ N L M A L H NK T K +I+ A ALG Sbjct: 302 AIATDG----LSSNYSLNMYEELKAALFVHPNKEATTFAKELIIRATKAGYDALG 352 >4X8F-A nrPDB Length = 309 Score = 26.9 bits (58), Expect = 4.4, Method: Compositional matrix adjust. Identities = 18/67 (26%), Positives = 28/67 (41%), Gaps = 1/67 (1%) Query: 94 IYHRNKSRATAIKGMIVATLFATAFALGLN-ALIVPLYFEAPFELYLKFFPFILAFNLVK 152 ++ R I G +V TA N AL+ L E P E +KF A ++ + Sbjct: 229 VWLSQNGRGQRIPGFVVKATDTTAAGDTFNGALVTGLLQEMPLESAIKFAHAAAAISVTR 288 Query: 153 FGIDSVV 159 FG + + Sbjct: 289 FGAQTSI 295 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5KBW-A nrPDB 198 1e-51 3P5N-A nrPDB 170 5e-43 Sequences not found previously or not previously below threshold: CONVERGED! >5KBW-A nrPDB Length = 181 Score = 198 bits (504), Expect = 1e-51, Method: Composition-based stats. Identities = 172/174 (98%), Positives = 172/174 (98%) Query: 2 SSIKKISFVGIFSALATLVMFLEFPIFPQASFLKYDPSEIPALIVSFLLGPGVGMFVVLV 61 SSIKKISFVGIFSALATLVMFLEFPIFPQASFLKYDPSEIPALIVSFLLGPGVGMFVVLV Sbjct: 3 SSIKKISFVGIFSALATLVMFLEFPIFPQASFLKYDPSEIPALIVSFLLGPGVGMFVVLV 62 Query: 62 KDILFFLMKSGDPVGIAMNAVLGMSFVGIAGLIYHRNKSRATAIKGMIVATLFATAFALG 121 KDILFFLMKSGDPVGIAMNAVLGMSFVGIAGLIYHRNKSRATAIKGMIVATLFATAFALG Sbjct: 63 KDILFFLMKSGDPVGIAMNAVLGMSFVGIAGLIYHRNKSRATAIKGMIVATLFATAFALG 122 Query: 122 LNALIVPLYFEAPFELYLKFFPFILAFNLVKFGIDSVVTFFVYKKVSSILKLET 175 LNALIVPLYFEAPFELYLKFFPFILAFNLVKFGIDSVVTFFVYKKVSSILKL Sbjct: 123 LNALIVPLYFEAPFELYLKFFPFILAFNLVKFGIDSVVTFFVYKKVSSILKLSN 176 >3P5N-A nrPDB Length = 189 Score = 170 bits (430), Expect = 5e-43, Method: Composition-based stats. Identities = 58/179 (32%), Positives = 100/179 (55%), Gaps = 7/179 (3%) Query: 1 MSSIKKISFVGIFSALATLVMFLEFPIFPQASFLKYDPSEIPALIVSFLLGPGVGMFVVL 60 M K++ + + SA+A ++ F++FPI +L D S++P+L+ +F GP G+ V L Sbjct: 9 MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSLLATFTFGPVAGIIVAL 68 Query: 61 VKDILFFLMKSGDPVGIAMNAVLGMSFVGIAGLIYHRNKSRATAIKGMIVATLFATAFAL 120 VK++L +L GDPVG N + G SF+ A IY +S + I G+I+AT+ T Sbjct: 69 VKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTKSLITGLIIATIVMTIVLS 128 Query: 121 GLNA-LIVPLY------FEAPFELYLKFFPFILAFNLVKFGIDSVVTFFVYKKVSSILK 172 LN +++PLY + L + I+ FN++K + S+V +Y+++++ LK Sbjct: 129 ILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKGIVISIVFILLYRRLANFLK 187 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.329 0.148 0.405 Lambda K H 0.267 0.0456 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,929,649 Number of Sequences: 36641 Number of extensions: 281441 Number of successful extensions: 875 Number of sequences better than 10.0: 27 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 33 Number of HSP's that attempted gapping in prelim test: 859 Number of HSP's gapped (non-prelim): 47 length of query: 183 length of database: 10,336,785 effective HSP length: 91 effective length of query: 92 effective length of database: 7,002,454 effective search space: 644225768 effective search space used: 644225768 T: 11 A: 40 X1: 16 ( 7.6 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 56 (26.0 bits)