BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9VAK8 (115 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3ZZR-A nrPDB 187 9e-49 5XVH-A nrPDB 28 1.0 5NZU-C nrPDB 27 1.9 3GBU-A nrPDB 26 4.5 5MHP-A nrPDB 26 5.1 6HV0-A nrPDB 25 7.0 >3ZZR-A nrPDB Length = 95 Score = 187 bits (475), Expect = 9e-49, Method: Compositional matrix adjust. Identities = 90/91 (98%), Positives = 90/91 (98%) Query: 25 ECCTSRELVEFKMDRGDCEAVRAIENYPNGCEVTICADGVAQLGAYCGQGSCNIFGCNCD 84 ECCTSRELVEFKMDRGDCEAVRAIENYPNGCEVTICADGVAQLGAYCGQG CNIFGCNCD Sbjct: 1 ECCTSRELVEFKMDRGDCEAVRAIENYPNGCEVTICADGVAQLGAYCGQGPCNIFGCNCD 60 Query: 85 GGCLSGDWSQEFVRRNQQYGIQIIKVTRLPF 115 GGCLSGDWSQEFVRRNQQYGIQIIKVTRLPF Sbjct: 61 GGCLSGDWSQEFVRRNQQYGIQIIKVTRLPF 91 >5XVH-A nrPDB Length = 306 Score = 28.1 bits (61), Expect = 1.0, Method: Compositional matrix adjust. Identities = 9/20 (45%), Positives = 14/20 (70%) Query: 89 SGDWSQEFVRRNQQYGIQII 108 S DW+++F R QYGI+ + Sbjct: 117 SPDWARKFAERLAQYGIEFL 136 >5NZU-C nrPDB Length = 905 Score = 26.9 bits (58), Expect = 1.9, Method: Composition-based stats. Identities = 12/27 (44%), Positives = 16/27 (59%), Gaps = 1/27 (3%) Query: 38 DRGDCEAV-RAIENYPNGCEVTICADG 63 D G CE + I++ PNG V +C DG Sbjct: 347 DMGSCEIYPQTIQHNPNGRFVVVCGDG 373 >3GBU-A nrPDB Length = 313 Score = 25.8 bits (55), Expect = 4.5, Method: Compositional matrix adjust. Identities = 11/32 (34%), Positives = 18/32 (56%), Gaps = 1/32 (3%) Query: 37 MDRGDCEAVRAIENYPNGCEVTICADGVAQLG 68 ++ GD + VR E +P G + GV++LG Sbjct: 17 VEEGDLKDVRLFEKHPGGAPANVAV-GVSRLG 47 >5MHP-A nrPDB Length = 871 Score = 25.8 bits (55), Expect = 5.1, Method: Composition-based stats. Identities = 12/32 (37%), Positives = 19/32 (59%), Gaps = 3/32 (9%) Query: 21 VARSECCTSRELV---EFKMDRGDCEAVRAIE 49 +AR +CCT+ ++V E DCE ++A E Sbjct: 125 LARGDCCTNYQVVCKGESHWVDDDCEEIKAAE 156 >6HV0-A nrPDB Length = 416 Score = 25.0 bits (53), Expect = 7.0, Method: Composition-based stats. Identities = 10/26 (38%), Positives = 17/26 (65%) Query: 9 LLVGICALAFVHVARSECCTSRELVE 34 +L+G C+L +H + E +REL+E Sbjct: 224 ILLGACSLDCLHPEKHEDVIARELIE 249 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3ZZR-A nrPDB 172 3e-44 Sequences not found previously or not previously below threshold: 5NZU-C nrPDB 26 2.9 5XVH-A nrPDB 26 3.4 2PLW-A nrPDB 26 3.5 5MHP-A nrPDB 25 8.5 3GBU-A nrPDB 25 9.7 CONVERGED! >3ZZR-A nrPDB Length = 95 Score = 172 bits (437), Expect = 3e-44, Method: Composition-based stats. Identities = 90/91 (98%), Positives = 90/91 (98%) Query: 25 ECCTSRELVEFKMDRGDCEAVRAIENYPNGCEVTICADGVAQLGAYCGQGSCNIFGCNCD 84 ECCTSRELVEFKMDRGDCEAVRAIENYPNGCEVTICADGVAQLGAYCGQG CNIFGCNCD Sbjct: 1 ECCTSRELVEFKMDRGDCEAVRAIENYPNGCEVTICADGVAQLGAYCGQGPCNIFGCNCD 60 Query: 85 GGCLSGDWSQEFVRRNQQYGIQIIKVTRLPF 115 GGCLSGDWSQEFVRRNQQYGIQIIKVTRLPF Sbjct: 61 GGCLSGDWSQEFVRRNQQYGIQIIKVTRLPF 91 >5NZU-C nrPDB Length = 905 Score = 26.4 bits (57), Expect = 2.9, Method: Composition-based stats. Identities = 12/27 (44%), Positives = 16/27 (59%), Gaps = 1/27 (3%) Query: 38 DRGDCEAV-RAIENYPNGCEVTICADG 63 D G CE + I++ PNG V +C DG Sbjct: 347 DMGSCEIYPQTIQHNPNGRFVVVCGDG 373 >5XVH-A nrPDB Length = 306 Score = 26.0 bits (56), Expect = 3.4, Method: Composition-based stats. Identities = 9/20 (45%), Positives = 14/20 (70%) Query: 89 SGDWSQEFVRRNQQYGIQII 108 S DW+++F R QYGI+ + Sbjct: 117 SPDWARKFAERLAQYGIEFL 136 >2PLW-A nrPDB Length = 201 Score = 26.0 bits (56), Expect = 3.5, Method: Composition-based stats. Identities = 10/29 (34%), Positives = 15/29 (51%) Query: 84 DGGCLSGDWSQEFVRRNQQYGIQIIKVTR 112 D GC G W Q + R + Y +II + + Sbjct: 28 DIGCYPGSWCQVILERTKNYKNKIIGIDK 56 >5MHP-A nrPDB Length = 871 Score = 24.9 bits (53), Expect = 8.5, Method: Composition-based stats. Identities = 12/32 (37%), Positives = 19/32 (59%), Gaps = 3/32 (9%) Query: 21 VARSECCTSRELV---EFKMDRGDCEAVRAIE 49 +AR +CCT+ ++V E DCE ++A E Sbjct: 125 LARGDCCTNYQVVCKGESHWVDDDCEEIKAAE 156 >3GBU-A nrPDB Length = 313 Score = 24.5 bits (52), Expect = 9.7, Method: Composition-based stats. Identities = 12/32 (37%), Positives = 19/32 (59%), Gaps = 1/32 (3%) Query: 37 MDRGDCEAVRAIENYPNGCEVTICADGVAQLG 68 ++ GD + VR E +P G + A GV++LG Sbjct: 17 VEEGDLKDVRLFEKHPGGAPANV-AVGVSRLG 47 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.323 0.147 0.497 Lambda K H 0.267 0.0442 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,476,513 Number of Sequences: 36641 Number of extensions: 239225 Number of successful extensions: 647 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 13 Number of HSP's that attempted gapping in prelim test: 634 Number of HSP's gapped (non-prelim): 27 length of query: 115 length of database: 10,336,785 effective HSP length: 80 effective length of query: 35 effective length of database: 7,405,505 effective search space: 259192675 effective search space used: 259192675 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.4 bits) S2: 53 (24.9 bits)