BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9V452 (140 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2BYK-A nrPDB 288 6e-79 5Y27-B nrPDB 49 7e-07 1N1J-B nrPDB 42 9e-05 5G49-B nrPDB 41 2e-04 4G92-C nrPDB 38 0.001 4WZS-A nrPDB 31 0.22 4FT4-B nrPDB 26 4.4 3DNF-A nrPDB 26 7.3 >2BYK-A nrPDB Length = 140 Score = 288 bits (736), Expect = 6e-79, Method: Compositional matrix adjust. Identities = 140/140 (100%), Positives = 140/140 (100%) Query: 1 MGEPRSQPPVERPPTAETFLPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGA 60 MGEPRSQPPVERPPTAETFLPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGA Sbjct: 1 MGEPRSQPPVERPPTAETFLPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGA 60 Query: 61 AYTEEFGQRPGEALKYEHLSQVVNKNKNLEFLLQIVPQKIRVHQFQEMLRLNRSAGSDDD 120 AYTEEFGQRPGEALKYEHLSQVVNKNKNLEFLLQIVPQKIRVHQFQEMLRLNRSAGSDDD Sbjct: 61 AYTEEFGQRPGEALKYEHLSQVVNKNKNLEFLLQIVPQKIRVHQFQEMLRLNRSAGSDDD 120 Query: 121 DDDDDDDDEEESESESESDE 140 DDDDDDDDEEESESESESDE Sbjct: 121 DDDDDDDDEEESESESESDE 140 >5Y27-B nrPDB Length = 92 Score = 48.9 bits (115), Expect = 7e-07, Method: Compositional matrix adjust. Identities = 30/78 (38%), Positives = 42/78 (53%), Gaps = 3/78 (3%) Query: 16 AETFLPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGAAY-TEEFGQRPGEAL 74 +T LPLSRV+ I+K D +N L++ TELFV LA AY + +R G + Sbjct: 11 GKTVLPLSRVKRIIKQDEDVHYCSNASALLISVATELFVEKLATEAYQLAKLQKRKG--I 68 Query: 75 KYEHLSQVVNKNKNLEFL 92 +Y + VV K+ EFL Sbjct: 69 RYRDVEDVVRKDDQFEFL 86 >1N1J-B nrPDB Length = 97 Score = 42.0 bits (97), Expect = 9e-05, Method: Compositional matrix adjust. Identities = 25/81 (30%), Positives = 43/81 (53%), Gaps = 5/81 (6%) Query: 20 LPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGAAY--TEEFGQRPGEALKYE 77 LPL+R++ IMK D +I+ E L K ++F+ L A+ TE+ +R L+ Sbjct: 20 LPLARIKKIMKLDEDVKMISAEAPVLFAKAAQIFITELTLRAWIHTEDNKRR---TLQRN 76 Query: 78 HLSQVVNKNKNLEFLLQIVPQ 98 ++ + K +FL+ IVP+ Sbjct: 77 DIAMAITKFDQFDFLIDIVPR 97 >5G49-B nrPDB Length = 95 Score = 40.8 bits (94), Expect = 2e-04, Method: Compositional matrix adjust. Identities = 24/81 (29%), Positives = 44/81 (54%), Gaps = 5/81 (6%) Query: 20 LPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGAAY--TEEFGQRPGEALKYE 77 LPL+R++ IMK+ D +I+ E + + E+F+ L ++ TEE +R L+ Sbjct: 18 LPLARIKKIMKADEDVRMISAEAPVVFARACEMFILELTLRSWNHTEENKRR---TLQKN 74 Query: 78 HLSQVVNKNKNLEFLLQIVPQ 98 ++ V + +FL+ IVP+ Sbjct: 75 DIAAAVTRTDIFDFLVDIVPR 95 >4G92-C nrPDB Length = 119 Score = 37.7 bits (86), Expect = 0.001, Method: Compositional matrix adjust. Identities = 22/80 (27%), Positives = 44/80 (55%), Gaps = 3/80 (3%) Query: 20 LPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGAAYTE-EFGQRPGEALKYEH 78 LPL+R++ +MK+ + +I+ E L K ++F+ L A+ E +R L+ Sbjct: 42 LPLARIKKVMKADPEVKMISAEAPILFAKGCDVFITELTMRAWIHAEDNKR--RTLQRSD 99 Query: 79 LSQVVNKNKNLEFLLQIVPQ 98 ++ ++K+ +FL+ IVP+ Sbjct: 100 IAAALSKSDMFDFLIDIVPR 119 >4WZS-A nrPDB Length = 95 Score = 30.8 bits (68), Expect = 0.22, Method: Compositional matrix adjust. Identities = 15/47 (31%), Positives = 25/47 (53%) Query: 18 TFLPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGAAYTE 64 T P+SR++ IM+ + D G I V + +K E+F+ + G E Sbjct: 12 TRFPISRLKRIMQLNEDIGKIGASVPVVASKAIEMFLTEIVGLTLKE 58 >4FT4-B nrPDB Length = 784 Score = 26.2 bits (56), Expect = 4.4, Method: Compositional matrix adjust. Identities = 27/109 (24%), Positives = 44/109 (40%), Gaps = 11/109 (10%) Query: 31 SSMDTGLITNEVLFLMTKCTELFVRHLAGAA---------YTEEFGQRPGEALKYEHLSQ 81 SSM T T +L L + C + GAA + +F ++LKY H Sbjct: 206 SSMPTRTAT--LLDLYSGCGGMSTGLCLGAALSGLKLETRWAVDFNSFACQSLKYNHPQT 263 Query: 82 VVNKNKNLEFLLQIVPQKIRVHQFQEMLRLNRSAGSDDDDDDDDDDDEE 130 V K EFL + + ++ + + N ++ D D+D D +E Sbjct: 264 EVRNEKADEFLALLKEWAVLCKKYVQDVDSNLASSEDQADEDSPLDKDE 312 >3DNF-A nrPDB Length = 297 Score = 25.8 bits (55), Expect = 7.3, Method: Compositional matrix adjust. Identities = 14/44 (31%), Positives = 22/44 (50%) Query: 53 FVRHLAGAAYTEEFGQRPGEALKYEHLSQVVNKNKNLEFLLQIV 96 ++R G E + GEALK+E + V +N EF ++V Sbjct: 133 YLRACNGKGIVVETLEDIGEALKHERVGIVAQTTQNEEFFKEVV 176 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2BYK-A nrPDB 216 2e-57 4G92-C nrPDB 118 1e-27 1N1J-B nrPDB 109 6e-25 5G49-B nrPDB 107 2e-24 5Y27-B nrPDB 100 3e-22 Sequences not found previously or not previously below threshold: 1JFI-A nrPDB 47 2e-06 4WZS-A nrPDB 45 1e-05 4FT4-B nrPDB 29 0.67 2EQO-A nrPDB 27 2.7 6FAI-l nrPDB 27 3.3 4CFG-A nrPDB 25 8.9 >2BYK-A nrPDB Length = 140 Score = 216 bits (551), Expect = 2e-57, Method: Composition-based stats. Identities = 140/140 (100%), Positives = 140/140 (100%) Query: 1 MGEPRSQPPVERPPTAETFLPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGA 60 MGEPRSQPPVERPPTAETFLPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGA Sbjct: 1 MGEPRSQPPVERPPTAETFLPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGA 60 Query: 61 AYTEEFGQRPGEALKYEHLSQVVNKNKNLEFLLQIVPQKIRVHQFQEMLRLNRSAGSDDD 120 AYTEEFGQRPGEALKYEHLSQVVNKNKNLEFLLQIVPQKIRVHQFQEMLRLNRSAGSDDD Sbjct: 61 AYTEEFGQRPGEALKYEHLSQVVNKNKNLEFLLQIVPQKIRVHQFQEMLRLNRSAGSDDD 120 Query: 121 DDDDDDDDEEESESESESDE 140 DDDDDDDDEEESESESESDE Sbjct: 121 DDDDDDDDEEESESESESDE 140 >4G92-C nrPDB Length = 119 Score = 118 bits (295), Expect = 1e-27, Method: Composition-based stats. Identities = 22/80 (27%), Positives = 44/80 (55%), Gaps = 3/80 (3%) Query: 20 LPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGAAYTE-EFGQRPGEALKYEH 78 LPL+R++ +MK+ + +I+ E L K ++F+ L A+ E +R L+ Sbjct: 42 LPLARIKKVMKADPEVKMISAEAPILFAKGCDVFITELTMRAWIHAEDNKR--RTLQRSD 99 Query: 79 LSQVVNKNKNLEFLLQIVPQ 98 ++ ++K+ +FL+ IVP+ Sbjct: 100 IAAALSKSDMFDFLIDIVPR 119 >1N1J-B nrPDB Length = 97 Score = 109 bits (271), Expect = 6e-25, Method: Composition-based stats. Identities = 24/80 (30%), Positives = 41/80 (51%), Gaps = 3/80 (3%) Query: 20 LPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGAAYTE-EFGQRPGEALKYEH 78 LPL+R++ IMK D +I+ E L K ++F+ L A+ E +R L+ Sbjct: 20 LPLARIKKIMKLDEDVKMISAEAPVLFAKAAQIFITELTLRAWIHTEDNKR--RTLQRND 77 Query: 79 LSQVVNKNKNLEFLLQIVPQ 98 ++ + K +FL+ IVP+ Sbjct: 78 IAMAITKFDQFDFLIDIVPR 97 >5G49-B nrPDB Length = 95 Score = 107 bits (266), Expect = 2e-24, Method: Composition-based stats. Identities = 24/81 (29%), Positives = 44/81 (54%), Gaps = 5/81 (6%) Query: 20 LPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGAAY--TEEFGQRPGEALKYE 77 LPL+R++ IMK+ D +I+ E + + E+F+ L ++ TEE +R L+ Sbjct: 18 LPLARIKKIMKADEDVRMISAEAPVVFARACEMFILELTLRSWNHTEENKRR---TLQKN 74 Query: 78 HLSQVVNKNKNLEFLLQIVPQ 98 ++ V + +FL+ IVP+ Sbjct: 75 DIAAAVTRTDIFDFLVDIVPR 95 >5Y27-B nrPDB Length = 92 Score = 100 bits (248), Expect = 3e-22, Method: Composition-based stats. Identities = 30/81 (37%), Positives = 43/81 (53%), Gaps = 3/81 (3%) Query: 16 AETFLPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGAAY-TEEFGQRPGEAL 74 +T LPLSRV+ I+K D +N L++ TELFV LA AY + +R G + Sbjct: 11 GKTVLPLSRVKRIIKQDEDVHYCSNASALLISVATELFVEKLATEAYQLAKLQKRKG--I 68 Query: 75 KYEHLSQVVNKNKNLEFLLQI 95 +Y + VV K+ EFL + Sbjct: 69 RYRDVEDVVRKDDQFEFLSDL 89 >1JFI-A nrPDB Length = 98 Score = 47.4 bits (111), Expect = 2e-06, Method: Composition-based stats. Identities = 15/64 (23%), Positives = 31/64 (48%), Gaps = 1/64 (1%) Query: 20 LPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGAAYTEEFGQRPGEALKYEHL 79 P +R++ IM++ + G + V ++++ ELF+ L A + R + + HL Sbjct: 12 FPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKA-CQVTQSRNAKTMTTSHL 70 Query: 80 SQVV 83 Q + Sbjct: 71 KQCI 74 >4WZS-A nrPDB Length = 95 Score = 45.1 bits (105), Expect = 1e-05, Method: Composition-based stats. Identities = 20/85 (23%), Positives = 37/85 (43%), Gaps = 1/85 (1%) Query: 8 PPVERPPTAETFLPLSRVRTIMKSSMDTGLITNEVLFLMTKCTELFVRHLAGAAYTEEFG 67 P E T P+SR++ IM+ + D G I V + +K E+F+ + G +E Sbjct: 2 PKQESQKKKLTRFPISRLKRIMQLNEDIGKIGASVPVVASKAIEMFLTEIVGLT-LKEAR 60 Query: 68 QRPGEALKYEHLSQVVNKNKNLEFL 92 ++ + E + + + FL Sbjct: 61 KKSSSRMSSEFIIRATESDPKFAFL 85 >4FT4-B nrPDB Length = 784 Score = 28.9 bits (63), Expect = 0.67, Method: Composition-based stats. Identities = 17/76 (22%), Positives = 31/76 (40%), Gaps = 1/76 (1%) Query: 55 RHLAGAAYTEEFGQRPGEALKYEHLSQVVNKNKNLEFLLQIVPQKIRVHQFQEMLRLNRS 114 L + +F ++LKY H V K EFL + + ++ + + N + Sbjct: 238 LKLETR-WAVDFNSFACQSLKYNHPQTEVRNEKADEFLALLKEWAVLCKKYVQDVDSNLA 296 Query: 115 AGSDDDDDDDDDDDEE 130 + D D+D D +E Sbjct: 297 SSEDQADEDSPLDKDE 312 >2EQO-A nrPDB Length = 140 Score = 27.0 bits (58), Expect = 2.7, Method: Composition-based stats. Identities = 14/65 (21%), Positives = 28/65 (43%) Query: 60 AAYTEEFGQRPGEALKYEHLSQVVNKNKNLEFLLQIVPQKIRVHQFQEMLRLNRSAGSDD 119 AA + G+ ++ L++ + +L I+ + IR+ F + L + SD+ Sbjct: 10 AAVVRRTQEALGKVIRRPPLTEKLLSKPPFRYLHDIITEVIRMTGFMKGLYTDAEMKSDN 69 Query: 120 DDDDD 124 D D Sbjct: 70 VKDKD 74 >6FAI-l nrPDB Length = 425 Score = 26.6 bits (57), Expect = 3.3, Method: Composition-based stats. Identities = 21/76 (27%), Positives = 31/76 (40%), Gaps = 7/76 (9%) Query: 71 GEALKYEHLSQVVNKNKNLEFLLQIV------PQKIRVHQFQEMLRLNRSAGSDDDDDDD 124 G Y + V + NL+ L+Q P + E +R N SDDD +D Sbjct: 303 GYKYAYPDFKRDVKRTDNLDELVQASGFSKKHPGDRGLETAVESMR-NAVYNSDDDMSND 361 Query: 125 DDDDEEESESESESDE 140 + ++E SE DE Sbjct: 362 EAEEENGEGDYSEEDE 377 >4CFG-A nrPDB Length = 630 Score = 25.4 bits (54), Expect = 8.9, Method: Composition-based stats. Identities = 6/27 (22%), Positives = 10/27 (37%) Query: 66 FGQRPGEALKYEHLSQVVNKNKNLEFL 92 ++ E + Q + K EFL Sbjct: 282 LKKKSEIQTLKEEIEQSLTKRDEFEFL 308 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.308 0.131 0.342 Lambda K H 0.267 0.0404 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,170,732 Number of Sequences: 36641 Number of extensions: 181189 Number of successful extensions: 2607 Number of sequences better than 10.0: 78 Number of HSP's better than 10.0 without gapping: 168 Number of HSP's successfully gapped in prelim test: 21 Number of HSP's that attempted gapping in prelim test: 961 Number of HSP's gapped (non-prelim): 1065 length of query: 140 length of database: 10,336,785 effective HSP length: 87 effective length of query: 53 effective length of database: 7,149,018 effective search space: 378897954 effective search space used: 378897954 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 54 (25.4 bits)