BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9UT27 (401 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5AX7-A nrPDB 725 0.0 2WW2-A nrPDB 31 0.79 5NCU-A nrPDB 31 1.0 5NCS-A nrPDB 31 1.2 4OV4-A nrPDB 28 8.0 >5AX7-A nrPDB Length = 348 Score = 725 bits (1872), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/348 (100%), Positives = 348/348 (100%) Query: 54 KKPLFTKSPRNSASCESTITLQSNLLFTYYKHYFAGIKKVALIGFPDHPNKGDSAIYVAE 113 KKPLFTKSPRNSASCESTITLQSNLLFTYYKHYFAGIKKVALIGFPDHPNKGDSAIYVAE Sbjct: 1 KKPLFTKSPRNSASCESTITLQSNLLFTYYKHYFAGIKKVALIGFPDHPNKGDSAIYVAE 60 Query: 114 KKLLDALNIEVVYITAQEADYSASELKSIISDIPRDEFALAFHGGGNFGDLYPDHQHLRE 173 KKLLDALNIEVVYITAQEADYSASELKSIISDIPRDEFALAFHGGGNFGDLYPDHQHLRE Sbjct: 61 KKLLDALNIEVVYITAQEADYSASELKSIISDIPRDEFALAFHGGGNFGDLYPDHQHLRE 120 Query: 174 LVVRDFPSFTTISFPQSVWYNEQQLLEQASILYAENPNITLVTRDRQSYGFAVDAFGKHN 233 LVVRDFPSFTTISFPQSVWYNEQQLLEQASILYAENPNITLVTRDRQSYGFAVDAFGKHN Sbjct: 121 LVVRDFPSFTTISFPQSVWYNEQQLLEQASILYAENPNITLVTRDRQSYGFAVDAFGKHN 180 Query: 234 EVLLTPDIVFFMGPIPEIREATPITHDVLILARLDHEGGQQHGAEDYYRDTLNAANLTYS 293 EVLLTPDIVFFMGPIPEIREATPITHDVLILARLDHEGGQQHGAEDYYRDTLNAANLTYS Sbjct: 181 EVLLTPDIVFFMGPIPEIREATPITHDVLILARLDHEGGQQHGAEDYYRDTLNAANLTYS 240 Query: 294 VEDWLLWDPPVAQNPDSSFDDRGQARYEAGAEFLASARVVITDRLHAHILSTLMGIPHIV 353 VEDWLLWDPPVAQNPDSSFDDRGQARYEAGAEFLASARVVITDRLHAHILSTLMGIPHIV Sbjct: 241 VEDWLLWDPPVAQNPDSSFDDRGQARYEAGAEFLASARVVITDRLHAHILSTLMGIPHIV 300 Query: 354 VENSQMGKITNYHNTWLHGCTLDGVSVVVDSVDKALSLLLEWNEAGYF 401 VENSQMGKITNYHNTWLHGCTLDGVSVVVDSVDKALSLLLEWNEAGYF Sbjct: 301 VENSQMGKITNYHNTWLHGCTLDGVSVVVDSVDKALSLLLEWNEAGYF 348 >2WW2-A nrPDB Length = 737 Score = 31.2 bits (69), Expect = 0.79, Method: Compositional matrix adjust. Identities = 23/71 (32%), Positives = 30/71 (42%), Gaps = 5/71 (7%) Query: 247 PIPEIREATPITHDV----LILARLDHEGGQQHGAEDYYRDTLNAANLTYSVEDWLLWDP 302 P PE+ EAT T + +AR G+Q A+ YY+ N NL Y E +W Sbjct: 437 PYPEVPEATAKTLEYAYADWCIARFAESLGKQDIADQYYQKAPNYRNLYYP-EHGFMWTK 495 Query: 303 PVAQNPDSSFD 313 N FD Sbjct: 496 DAKGNWRDRFD 506 >5NCU-A nrPDB Length = 336 Score = 30.8 bits (68), Expect = 1.0, Method: Compositional matrix adjust. Identities = 19/63 (30%), Positives = 33/63 (52%), Gaps = 2/63 (3%) Query: 144 SDIPRDEFALAFHGGGNFGDLYPDHQH-LRELVVRDFPSFTTISFPQSVWYNEQQLLEQA 202 + + DE A G + ++ H LRE +++ PS TTI S+WY + +L+++ Sbjct: 50 AGVTADEMKTALRETGYTMEDINEYSHSLREALLKVDPS-TTIGMANSIWYKQGELVKEP 108 Query: 203 SIL 205 IL Sbjct: 109 FIL 111 >5NCS-A nrPDB Length = 375 Score = 30.8 bits (68), Expect = 1.2, Method: Compositional matrix adjust. Identities = 19/63 (30%), Positives = 33/63 (52%), Gaps = 2/63 (3%) Query: 144 SDIPRDEFALAFHGGGNFGDLYPDHQH-LRELVVRDFPSFTTISFPQSVWYNEQQLLEQA 202 + + DE A G + ++ H LRE +++ PS TTI S+WY + +L+++ Sbjct: 50 AGVTADEMKTALRETGYTMEDINEYSHSLREALLKVDPS-TTIGMANSIWYKQGELVKEP 108 Query: 203 SIL 205 IL Sbjct: 109 FIL 111 >4OV4-A nrPDB Length = 414 Score = 28.1 bits (61), Expect = 8.0, Method: Compositional matrix adjust. Identities = 17/64 (26%), Positives = 31/64 (48%), Gaps = 5/64 (7%) Query: 116 LLDALNIEVVYITAQEADYSASELKSIISDIPRDEFALAFHGGG-----NFGDLYPDHQH 170 + A ++E VY+ A++ + + + L + E A+A H G NF +YP + Sbjct: 234 MATATSVESVYVGAEQIEVALNGLGERAGNTNLYETAIALHQNGENLNINFQRIYPTAKR 293 Query: 171 LREL 174 + EL Sbjct: 294 ISEL 297 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5AX7-A nrPDB 748 0.0 Sequences not found previously or not previously below threshold: 2WW2-A nrPDB 33 0.31 5NCU-A nrPDB 30 1.6 5NCS-A nrPDB 30 1.6 1OJM-A nrPDB 30 2.1 5WLN-G nrPDB 29 3.2 2GW3-A nrPDB 28 5.1 4HOQ-A nrPDB 28 5.6 4OV4-A nrPDB 28 6.5 CONVERGED! >5AX7-A nrPDB Length = 348 Score = 748 bits (1932), Expect = 0.0, Method: Composition-based stats. Identities = 348/348 (100%), Positives = 348/348 (100%) Query: 54 KKPLFTKSPRNSASCESTITLQSNLLFTYYKHYFAGIKKVALIGFPDHPNKGDSAIYVAE 113 KKPLFTKSPRNSASCESTITLQSNLLFTYYKHYFAGIKKVALIGFPDHPNKGDSAIYVAE Sbjct: 1 KKPLFTKSPRNSASCESTITLQSNLLFTYYKHYFAGIKKVALIGFPDHPNKGDSAIYVAE 60 Query: 114 KKLLDALNIEVVYITAQEADYSASELKSIISDIPRDEFALAFHGGGNFGDLYPDHQHLRE 173 KKLLDALNIEVVYITAQEADYSASELKSIISDIPRDEFALAFHGGGNFGDLYPDHQHLRE Sbjct: 61 KKLLDALNIEVVYITAQEADYSASELKSIISDIPRDEFALAFHGGGNFGDLYPDHQHLRE 120 Query: 174 LVVRDFPSFTTISFPQSVWYNEQQLLEQASILYAENPNITLVTRDRQSYGFAVDAFGKHN 233 LVVRDFPSFTTISFPQSVWYNEQQLLEQASILYAENPNITLVTRDRQSYGFAVDAFGKHN Sbjct: 121 LVVRDFPSFTTISFPQSVWYNEQQLLEQASILYAENPNITLVTRDRQSYGFAVDAFGKHN 180 Query: 234 EVLLTPDIVFFMGPIPEIREATPITHDVLILARLDHEGGQQHGAEDYYRDTLNAANLTYS 293 EVLLTPDIVFFMGPIPEIREATPITHDVLILARLDHEGGQQHGAEDYYRDTLNAANLTYS Sbjct: 181 EVLLTPDIVFFMGPIPEIREATPITHDVLILARLDHEGGQQHGAEDYYRDTLNAANLTYS 240 Query: 294 VEDWLLWDPPVAQNPDSSFDDRGQARYEAGAEFLASARVVITDRLHAHILSTLMGIPHIV 353 VEDWLLWDPPVAQNPDSSFDDRGQARYEAGAEFLASARVVITDRLHAHILSTLMGIPHIV Sbjct: 241 VEDWLLWDPPVAQNPDSSFDDRGQARYEAGAEFLASARVVITDRLHAHILSTLMGIPHIV 300 Query: 354 VENSQMGKITNYHNTWLHGCTLDGVSVVVDSVDKALSLLLEWNEAGYF 401 VENSQMGKITNYHNTWLHGCTLDGVSVVVDSVDKALSLLLEWNEAGYF Sbjct: 301 VENSQMGKITNYHNTWLHGCTLDGVSVVVDSVDKALSLLLEWNEAGYF 348 >2WW2-A nrPDB Length = 737 Score = 32.7 bits (73), Expect = 0.31, Method: Composition-based stats. Identities = 23/71 (32%), Positives = 30/71 (42%), Gaps = 5/71 (7%) Query: 247 PIPEIREATPITHDVL----ILARLDHEGGQQHGAEDYYRDTLNAANLTYSVEDWLLWDP 302 P PE+ EAT T + +AR G+Q A+ YY+ N NL Y E +W Sbjct: 437 PYPEVPEATAKTLEYAYADWCIARFAESLGKQDIADQYYQKAPNYRNLYYP-EHGFMWTK 495 Query: 303 PVAQNPDSSFD 313 N FD Sbjct: 496 DAKGNWRDRFD 506 >5NCU-A nrPDB Length = 336 Score = 30.0 bits (66), Expect = 1.6, Method: Composition-based stats. Identities = 19/61 (31%), Positives = 32/61 (52%), Gaps = 2/61 (3%) Query: 146 IPRDEFALAFHGGGNFGDLYPDHQH-LRELVVRDFPSFTTISFPQSVWYNEQQLLEQASI 204 + DE A G + ++ H LRE +++ PS TTI S+WY + +L+++ I Sbjct: 52 VTADEMKTALRETGYTMEDINEYSHSLREALLKVDPS-TTIGMANSIWYKQGELVKEPFI 110 Query: 205 L 205 L Sbjct: 111 L 111 >5NCS-A nrPDB Length = 375 Score = 30.0 bits (66), Expect = 1.6, Method: Composition-based stats. Identities = 19/61 (31%), Positives = 32/61 (52%), Gaps = 2/61 (3%) Query: 146 IPRDEFALAFHGGGNFGDLYPDHQH-LRELVVRDFPSFTTISFPQSVWYNEQQLLEQASI 204 + DE A G + ++ H LRE +++ PS TTI S+WY + +L+++ I Sbjct: 52 VTADEMKTALRETGYTMEDINEYSHSLREALLKVDPS-TTIGMANSIWYKQGELVKEPFI 110 Query: 205 L 205 L Sbjct: 111 L 111 >1OJM-A nrPDB Length = 729 Score = 30.0 bits (66), Expect = 2.1, Method: Composition-based stats. Identities = 25/113 (22%), Positives = 47/113 (41%), Gaps = 10/113 (8%) Query: 27 GVTRTDLQTLKNPSSLTSPSSSTSVDKKKPLFTKSPRNSASCESTITLQS-NLLFTYYKH 85 G T TD + + + PS+ K + A+ +T+ + N T +K Sbjct: 450 GTTETDAKRADSDTGKVLPSAFVGTSK---------LDDANATATMDFTNWNQTLTAHKS 500 Query: 86 YFAGIKKVALIGFPDHPNKGDSAIYVAEKKLLDALNIEVVYITAQEADYSASE 138 +F K+A +G D+A +++ L++ N VY+ +EA + E Sbjct: 501 WFMLKDKIAFLGSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQE 553 >5WLN-G nrPDB Length = 624 Score = 29.2 bits (64), Expect = 3.2, Method: Composition-based stats. Identities = 24/65 (36%), Positives = 32/65 (49%), Gaps = 15/65 (23%) Query: 274 QHGAEDYYRDTLNAANLTYSVEDWLLWDPPVAQNPDSSFDDRGQARYEAGAEFLASARVV 333 Q G+ DY + NL Y W++ V N S RGQA+ AGA+ +A AR Sbjct: 169 QKGSHDY-----SVINLRYG---WVMDAAEVLNNAMS----RGQAKGAAGAQVIADAR-- 214 Query: 334 ITDRL 338 T+RL Sbjct: 215 -TNRL 218 >2GW3-A nrPDB Length = 227 Score = 28.5 bits (62), Expect = 5.1, Method: Composition-based stats. Identities = 19/66 (28%), Positives = 25/66 (37%), Gaps = 11/66 (16%) Query: 149 DEFALAFHGGGNFGDLYPDHQHLRELVVRDFPSFTTISFPQSVWYNEQQLLEQASILYAE 208 D AFH G YPDH P + SFP+ + + E + A Sbjct: 57 DILTTAFHYGNRVFAKYPDH----------IPDYFKQSFPKGFSWERSLMFEDGGVCIAT 106 Query: 209 NPNITL 214 N +ITL Sbjct: 107 N-DITL 111 >4HOQ-A nrPDB Length = 482 Score = 28.5 bits (62), Expect = 5.6, Method: Composition-based stats. Identities = 13/28 (46%), Positives = 16/28 (57%) Query: 264 LARLDHEGGQQHGAEDYYRDTLNAANLT 291 LA + EGGQ AED +R L N+T Sbjct: 344 LANMYAEGGQYSNAEDIFRKALRLENIT 371 >4OV4-A nrPDB Length = 414 Score = 28.1 bits (61), Expect = 6.5, Method: Composition-based stats. Identities = 17/61 (27%), Positives = 30/61 (49%), Gaps = 5/61 (8%) Query: 119 ALNIEVVYITAQEADYSASELKSIISDIPRDEFALAFHGGG-----NFGDLYPDHQHLRE 173 A ++E VY+ A++ + + + L + E A+A H G NF +YP + + E Sbjct: 237 ATSVESVYVGAEQIEVALNGLGERAGNTNLYETAIALHQNGENLNINFQRIYPTAKRISE 296 Query: 174 L 174 L Sbjct: 297 L 297 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.319 0.135 0.404 Lambda K H 0.267 0.0428 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 17,865,381 Number of Sequences: 36641 Number of extensions: 771483 Number of successful extensions: 1635 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 1630 Number of HSP's gapped (non-prelim): 19 length of query: 401 length of database: 10,336,785 effective HSP length: 99 effective length of query: 302 effective length of database: 6,709,326 effective search space: 2026216452 effective search space used: 2026216452 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 60 (27.7 bits)