BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9USH1 (139 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5U0P-3 nrPDB 286 2e-78 3FBN-B nrPDB 64 2e-11 5OQM-o nrPDB 64 2e-11 1TON-A nrPDB 28 0.93 6HIV-Cd nrPDB 26 6.4 5Z0U-A nrPDB 25 8.7 6OKK-O nrPDB 25 9.5 >5U0P-3 nrPDB Length = 139 Score = 286 bits (732), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 139/139 (100%), Positives = 139/139 (100%) Query: 1 METKWLLSKVPDDKSRFEIELEFVQMLSNPWYLNFLAQHKYFEDEAFLQYLEYMEYWREP 60 METKWLLSKVPDDKSRFEIELEFVQMLSNPWYLNFLAQHKYFEDEAFLQYLEYMEYWREP Sbjct: 1 METKWLLSKVPDDKSRFEIELEFVQMLSNPWYLNFLAQHKYFEDEAFLQYLEYMEYWREP 60 Query: 61 EYVKFIIYPTCLHMLTLLKNPQFRNDISRADLSKQVNDEIYYEWLGKGLQQYGSADDATL 120 EYVKFIIYPTCLHMLTLLKNPQFRNDISRADLSKQVNDEIYYEWLGKGLQQYGSADDATL Sbjct: 61 EYVKFIIYPTCLHMLTLLKNPQFRNDISRADLSKQVNDEIYYEWLGKGLQQYGSADDATL 120 Query: 121 SQPQQEEDEKKVDVKKENE 139 SQPQQEEDEKKVDVKKENE Sbjct: 121 SQPQQEEDEKKVDVKKENE 139 >3FBN-B nrPDB Length = 130 Score = 64.3 bits (155), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 30/67 (44%), Positives = 44/67 (65%), Gaps = 1/67 (1%) Query: 15 SRFEIELEFVQMLSNPWYLNFL-AQHKYFEDEAFLQYLEYMEYWREPEYVKFIIYPTCLH 73 +RFE+ELEF+Q L+N Y+ +L Q + ++ F YL+Y+EYW P Y + I+YP CL Sbjct: 24 TRFEVELEFIQSLANIQYVTYLLTQQQIWKSPNFKNYLKYLEYWCNPPYSQCIVYPNCLF 83 Query: 74 MLTLLKN 80 +L LL Sbjct: 84 ILKLLNG 90 >5OQM-o nrPDB Length = 151 Score = 64.3 bits (155), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 30/67 (44%), Positives = 44/67 (65%), Gaps = 1/67 (1%) Query: 15 SRFEIELEFVQMLSNPWYLNFL-AQHKYFEDEAFLQYLEYMEYWREPEYVKFIIYPTCLH 73 +RFE+ELEF+Q L+N Y+ +L Q + ++ F YL+Y+EYW P Y + I+YP CL Sbjct: 21 TRFEVELEFIQSLANIQYVTYLLTQQQIWKSPNFKNYLKYLEYWCNPPYSQCIVYPNCLF 80 Query: 74 MLTLLKN 80 +L LL Sbjct: 81 ILKLLNG 87 >1TON-A nrPDB Length = 235 Score = 28.5 bits (62), Expect = 0.93, Method: Compositional matrix adjust. Identities = 11/36 (30%), Positives = 19/36 (52%) Query: 32 YLNFLAQHKYFEDEAFLQYLEYMEYWREPEYVKFII 67 Y L ++ F+DE F Q + +R P+Y+ I+ Sbjct: 45 YQVLLGRNNLFKDEPFAQRRLVRQSFRHPDYIPLIV 80 >6HIV-Cd nrPDB Length = 440 Score = 25.8 bits (55), Expect = 6.4, Method: Compositional matrix adjust. Identities = 33/136 (24%), Positives = 59/136 (43%), Gaps = 12/136 (8%) Query: 6 LLSKVPDDKSRFEIELE---FVQMLSNPWYLNFLAQHKYFE--DEAFLQYLEYMEYWREP 60 L++ VP + R I+ E +V + P+ LNF+ Q + E D ++ E + Sbjct: 147 LINYVPHAQLREHIDKELDKYVAGKNEPYPLNFVGQMPFLEDGDGNIVEVPESLLSNHMA 206 Query: 61 EYVKFIIYPTCLHMLTLLKN-PQFRNDISRADLSKQVNDEIYYEWLGKGLQQYGSADDAT 119 E+ P H + + F + RA +S +E EWLG + A T Sbjct: 207 EHPDSTAKPHEPHTSSSISEAAAFEERMLRAMVS--AKEEDLKEWLGDDSR----ALSET 260 Query: 120 LSQPQQEEDEKKVDVK 135 + +EE+E++ D++ Sbjct: 261 IDDISREEEEREADIR 276 >5Z0U-A nrPDB Length = 626 Score = 25.4 bits (54), Expect = 8.7, Method: Composition-based stats. Identities = 21/80 (26%), Positives = 38/80 (47%), Gaps = 4/80 (5%) Query: 28 SNPWYLNFLAQHKYFEDEA-FLQY--LEYMEYWREPEYVKFIIYPTCLHMLTLLKNPQFR 84 S+PWY N+ + + + A FL + L + Y V+ +IY + NP + Sbjct: 291 SSPWY-NYYTFYTWPDSYASFLGFNSLPKLNYGNSGSAVRGVIYNNSNSVAKTYLNPPYS 349 Query: 85 NDISRADLSKQVNDEIYYEW 104 D R D ++ V+ +I+ E+ Sbjct: 350 VDGWRLDAAQYVDHQIWSEF 369 >6OKK-O nrPDB Length = 137 Score = 25.4 bits (54), Expect = 9.5, Method: Compositional matrix adjust. Identities = 20/82 (24%), Positives = 38/82 (46%) Query: 8 SKVPDDKSRFEIELEFVQMLSNPWYLNFLAQHKYFEDEAFLQYLEYMEYWREPEYVKFII 67 +K+P L + L + N++ + ++ + F+ E +EY RE ++ I Sbjct: 38 AKIPRHPHLNVPNLHIMMTLKSLKSRNYVEEKYNWKHQYFILNNEGIEYLREFLHLPPSI 97 Query: 68 YPTCLHMLTLLKNPQFRNDISR 89 +P L T+ + P+ DISR Sbjct: 98 FPATLSKKTVNRAPKMDEDISR 119 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5U0P-3 nrPDB 248 6e-67 3FBN-B nrPDB 120 1e-28 5OQM-o nrPDB 119 3e-28 Sequences not found previously or not previously below threshold: 6OKK-O nrPDB 30 0.35 5N77-A nrPDB 27 2.1 6B43-6 nrPDB 27 3.3 1UJS-A nrPDB 26 4.7 4DQ1-A nrPDB 26 5.4 1RY2-A nrPDB 26 7.1 6C23-K nrPDB 25 10.0 1R03-A nrPDB 25 10.0 CONVERGED! >5U0P-3 nrPDB Length = 139 Score = 248 bits (633), Expect = 6e-67, Method: Composition-based stats. Identities = 139/139 (100%), Positives = 139/139 (100%) Query: 1 METKWLLSKVPDDKSRFEIELEFVQMLSNPWYLNFLAQHKYFEDEAFLQYLEYMEYWREP 60 METKWLLSKVPDDKSRFEIELEFVQMLSNPWYLNFLAQHKYFEDEAFLQYLEYMEYWREP Sbjct: 1 METKWLLSKVPDDKSRFEIELEFVQMLSNPWYLNFLAQHKYFEDEAFLQYLEYMEYWREP 60 Query: 61 EYVKFIIYPTCLHMLTLLKNPQFRNDISRADLSKQVNDEIYYEWLGKGLQQYGSADDATL 120 EYVKFIIYPTCLHMLTLLKNPQFRNDISRADLSKQVNDEIYYEWLGKGLQQYGSADDATL Sbjct: 61 EYVKFIIYPTCLHMLTLLKNPQFRNDISRADLSKQVNDEIYYEWLGKGLQQYGSADDATL 120 Query: 121 SQPQQEEDEKKVDVKKENE 139 SQPQQEEDEKKVDVKKENE Sbjct: 121 SQPQQEEDEKKVDVKKENE 139 >3FBN-B nrPDB Length = 130 Score = 120 bits (302), Expect = 1e-28, Method: Composition-based stats. Identities = 30/67 (44%), Positives = 44/67 (65%), Gaps = 1/67 (1%) Query: 15 SRFEIELEFVQMLSNPWYLNFL-AQHKYFEDEAFLQYLEYMEYWREPEYVKFIIYPTCLH 73 +RFE+ELEF+Q L+N Y+ +L Q + ++ F YL+Y+EYW P Y + I+YP CL Sbjct: 24 TRFEVELEFIQSLANIQYVTYLLTQQQIWKSPNFKNYLKYLEYWCNPPYSQCIVYPNCLF 83 Query: 74 MLTLLKN 80 +L LL Sbjct: 84 ILKLLNG 90 >5OQM-o nrPDB Length = 151 Score = 119 bits (299), Expect = 3e-28, Method: Composition-based stats. Identities = 30/67 (44%), Positives = 44/67 (65%), Gaps = 1/67 (1%) Query: 15 SRFEIELEFVQMLSNPWYLNFL-AQHKYFEDEAFLQYLEYMEYWREPEYVKFIIYPTCLH 73 +RFE+ELEF+Q L+N Y+ +L Q + ++ F YL+Y+EYW P Y + I+YP CL Sbjct: 21 TRFEVELEFIQSLANIQYVTYLLTQQQIWKSPNFKNYLKYLEYWCNPPYSQCIVYPNCLF 80 Query: 74 MLTLLKN 80 +L LL Sbjct: 81 ILKLLNG 87 >6OKK-O nrPDB Length = 137 Score = 30.0 bits (66), Expect = 0.35, Method: Composition-based stats. Identities = 18/69 (26%), Positives = 34/69 (49%) Query: 21 LEFVQMLSNPWYLNFLAQHKYFEDEAFLQYLEYMEYWREPEYVKFIIYPTCLHMLTLLKN 80 L + L + N++ + ++ + F+ E +EY RE ++ I+P L T+ + Sbjct: 51 LHIMMTLKSLKSRNYVEEKYNWKHQYFILNNEGIEYLREFLHLPPSIFPATLSKKTVNRA 110 Query: 81 PQFRNDISR 89 P+ DISR Sbjct: 111 PKMDEDISR 119 >5N77-A nrPDB Length = 257 Score = 27.3 bits (59), Expect = 2.1, Method: Composition-based stats. Identities = 28/103 (27%), Positives = 39/103 (37%), Gaps = 6/103 (5%) Query: 5 WLLSKVPDDKSRFEIELEFVQMLSN-PWYLNFLAQHKYFEDEAFLQ-----YLEYMEYWR 58 W+ PDD R ++ E Q L+ P + A ++FED+ L + E E Sbjct: 28 WIDLVEPDDDERLRVQSELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHA 87 Query: 59 EPEYVKFIIYPTCLHMLTLLKNPQFRNDISRADLSKQVNDEIY 101 V F I L L + P FR RA V+ Y Sbjct: 88 GNSTVAFTIRDGRLFTLRERELPAFRLYRMRARSQSMVDGNAY 130 >6B43-6 nrPDB Length = 305 Score = 26.5 bits (57), Expect = 3.3, Method: Composition-based stats. Identities = 9/24 (37%), Positives = 15/24 (62%) Query: 10 VPDDKSRFEIELEFVQMLSNPWYL 33 VPDD +R + ++Q LS+ + L Sbjct: 259 VPDDITRMRVMFSYLQSLSSIFNL 282 >1UJS-A nrPDB Length = 88 Score = 26.2 bits (56), Expect = 4.7, Method: Composition-based stats. Identities = 14/56 (25%), Positives = 26/56 (46%), Gaps = 3/56 (5%) Query: 57 WREPEYVKFIIYPTCLHMLTLLKNPQFRNDISRADLSKQVNDEIYYEWLGKGLQQY 112 W EY IYP L ++T + D+ R L + ++ E +Y+ G + ++ Sbjct: 12 WGMREYK---IYPYELLLVTTRGRNRLPKDVDRTRLERHLSQEEFYQVFGMTISEF 64 >4DQ1-A nrPDB Length = 321 Score = 26.2 bits (56), Expect = 5.4, Method: Composition-based stats. Identities = 10/36 (27%), Positives = 18/36 (50%), Gaps = 1/36 (2%) Query: 21 LEFVQMLSNPWYLNFLAQHKYFEDEAFLQYLEYMEY 56 L F++ +N YL + + + AF Y++ EY Sbjct: 68 LWFIKGDTNIQYL-LKYNNNIWNEWAFENYIKSDEY 102 >1RY2-A nrPDB Length = 663 Score = 25.8 bits (55), Expect = 7.1, Method: Composition-based stats. Identities = 9/33 (27%), Positives = 15/33 (45%) Query: 44 DEAFLQYLEYMEYWREPEYVKFIIYPTCLHMLT 76 A+ Y Y + E + +F + PT L +L Sbjct: 339 TPAYPNYSRYWDIIDEHKVTQFYVAPTALRLLK 371 >6C23-K nrPDB Length = 746 Score = 25.0 bits (53), Expect = 10.0, Method: Composition-based stats. Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 1/44 (2%) Query: 96 VNDEIYYEWLGKGLQQYGSADDATLSQPQQEEDEKKVDVKKENE 139 +NDEI+ E L L QY DD +E +EK+ D++ + Sbjct: 166 INDEIFVE-LVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRD 208 >1R03-A nrPDB Length = 182 Score = 25.0 bits (53), Expect = 10.0, Method: Composition-based stats. Identities = 11/34 (32%), Positives = 20/34 (58%) Query: 88 SRADLSKQVNDEIYYEWLGKGLQQYGSADDATLS 121 S A +++Q+N E+Y ++ + Y S DD L+ Sbjct: 16 SEAAINRQINLELYASYVYLSMAYYFSRDDVALN 49 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.312 0.136 0.403 Lambda K H 0.267 0.0424 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,031,444 Number of Sequences: 36641 Number of extensions: 240602 Number of successful extensions: 644 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 15 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 621 Number of HSP's gapped (non-prelim): 25 length of query: 139 length of database: 10,336,785 effective HSP length: 87 effective length of query: 52 effective length of database: 7,149,018 effective search space: 371748936 effective search space used: 371748936 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.3 bits) S2: 54 (25.4 bits)