BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9US45 (216 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5U0S-F nrPDB 452 e-128 4H61-A nrPDB 361 e-100 3RJ1-G nrPDB 102 1e-22 3QP5-A nrPDB 29 1.7 3UA4-A nrPDB 27 5.8 5OGZ-A nrPDB 27 7.7 2Z1S-A nrPDB 27 8.6 3QP6-A nrPDB 27 9.1 >5U0S-F nrPDB Length = 216 Score = 452 bits (1162), Expect = e-128, Method: Compositional matrix adjust. Identities = 216/216 (100%), Positives = 216/216 (100%) Query: 1 MASGAPPSVDLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNA 60 MASGAPPSVDLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNA Sbjct: 1 MASGAPPSVDLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNA 60 Query: 61 LDLGDLNSQLKRLTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPN 120 LDLGDLNSQLKRLTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPN Sbjct: 61 LDLGDLNSQLKRLTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPN 120 Query: 121 AYTLLATRMLNATYCFQKALTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTNEPADENK 180 AYTLLATRMLNATYCFQKALTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTNEPADENK Sbjct: 121 AYTLLATRMLNATYCFQKALTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTNEPADENK 180 Query: 181 NQSIENADYSFSPEDFSVVRAFMQSLHSSKEAPDVK 216 NQSIENADYSFSPEDFSVVRAFMQSLHSSKEAPDVK Sbjct: 181 NQSIENADYSFSPEDFSVVRAFMQSLHSSKEAPDVK 216 >4H61-A nrPDB Length = 175 Score = 361 bits (927), Expect = e-100, Method: Compositional matrix adjust. Identities = 171/172 (99%), Positives = 172/172 (100%) Query: 9 VDLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNALDLGDLNS 68 +DLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNALDLGDLNS Sbjct: 4 MDLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNALDLGDLNS 63 Query: 69 QLKRLTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPNAYTLLATR 128 QLKRLTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPNAYTLLATR Sbjct: 64 QLKRLTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPNAYTLLATR 123 Query: 129 MLNATYCFQKALTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTNEPADENK 180 MLNATYCFQKALTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTNEPADENK Sbjct: 124 MLNATYCFQKALTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTNEPADENK 175 >3RJ1-G nrPDB Length = 295 Score = 102 bits (254), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 59/198 (29%), Positives = 93/198 (46%), Gaps = 52/198 (26%) Query: 11 LTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQF------------ 58 L +QW+ PEW+Q G LRTENVL+YF++SPF+ SNN+++KMQ QF Sbjct: 6 LDELQWKSPEWIQVFG-LRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNAAVNM 64 Query: 59 --NALDLGD-----------------------------LNSQLKRLTGIQFVIIHERPPF 87 N + L D L +L +L G ++V+ R P Sbjct: 65 TQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSVREPD 124 Query: 88 LWVIQKQNRLNEN--------EVKPLTVYFVCNENIYMAPNAYTLLATRMLNATYCFQKA 139 WVI+KQ R N + E+ PL Y++ NIY +P + ++ +R+++ +Y Sbjct: 125 FWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYHLNST 184 Query: 140 LTKIEKFPQYNPQEGYTY 157 L + ++ P +G Y Sbjct: 185 LESLYDLIEFQPSQGVHY 202 >3QP5-A nrPDB Length = 265 Score = 28.9 bits (63), Expect = 1.7, Method: Compositional matrix adjust. Identities = 24/74 (32%), Positives = 34/74 (45%), Gaps = 21/74 (28%) Query: 7 PSVDLTSIQWRMPEWVQSMGGLRTENVLEYF-----SQSPFYSHKSNNEMLKMQSQFNAL 61 P+ S QW + EW+ G + TEN L+ F SQ+P S+ L Sbjct: 13 PAGLTASQQWTLLEWIHMAGHIETENELKAFLDQVLSQAP--------------SERLLL 58 Query: 62 DLGDLN--SQLKRL 73 LG LN +Q++RL Sbjct: 59 ALGRLNNQNQIQRL 72 >3UA4-A nrPDB Length = 745 Score = 26.9 bits (58), Expect = 5.8, Method: Compositional matrix adjust. Identities = 22/97 (22%), Positives = 41/97 (42%), Gaps = 5/97 (5%) Query: 2 ASGAPPSVDLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNAL 61 A PP +DL +Q R W + G + ++ S P ++ S +LK S L Sbjct: 103 AENHPPVIDLPDVQLRNDLWESYVVG-KISPWIDCDSSDPAFASLSEEHLLKELSYICYL 161 Query: 62 DLGDLNSQLKRLTGIQFVIIHERPPFLWVIQKQNRLN 98 L + +L R++ + I ++ W+ + +R Sbjct: 162 GLQTMAIELTRISSPRTAAILKK----WIWTRNSRFT 194 >5OGZ-A nrPDB Length = 456 Score = 26.6 bits (57), Expect = 7.7, Method: Compositional matrix adjust. Identities = 10/39 (25%), Positives = 22/39 (56%), Gaps = 2/39 (5%) Query: 1 MASGAPPSVDLTSIQWRMPEWVQSMGGLRTENVLEYFSQ 39 + +G P++ L W +P+ +Q GG + + +YF++ Sbjct: 109 LENGIMPAITLY--HWDLPQKLQDKGGWKNRDTTDYFTE 145 >2Z1S-A nrPDB Length = 454 Score = 26.6 bits (57), Expect = 8.6, Method: Compositional matrix adjust. Identities = 8/27 (29%), Positives = 15/27 (55%) Query: 11 LTSIQWRMPEWVQSMGGLRTENVLEYF 37 LT W +P+W++ GG +++F Sbjct: 124 LTLYHWDLPQWIEDEGGWTQRETIQHF 150 >3QP6-A nrPDB Length = 265 Score = 26.6 bits (57), Expect = 9.1, Method: Compositional matrix adjust. Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 5/40 (12%) Query: 7 PSVDLTSIQWRMPEWVQSMGGLRTENVLEYF-----SQSP 41 P+ S QW + EW+ G + TE L+ F SQ+P Sbjct: 13 PAGLTASQQWTLLEWIHMAGHIETEGELKAFLDNILSQAP 52 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5U0S-F nrPDB 403 e-113 4H61-A nrPDB 321 2e-88 3RJ1-G nrPDB 218 2e-57 Sequences not found previously or not previously below threshold: 3QP5-A nrPDB 32 0.17 1PD0-A nrPDB 29 1.9 1M2V-B nrPDB 28 2.9 1DPE-A nrPDB 27 4.9 CONVERGED! >5U0S-F nrPDB Length = 216 Score = 403 bits (1036), Expect = e-113, Method: Composition-based stats. Identities = 216/216 (100%), Positives = 216/216 (100%) Query: 1 MASGAPPSVDLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNA 60 MASGAPPSVDLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNA Sbjct: 1 MASGAPPSVDLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNA 60 Query: 61 LDLGDLNSQLKRLTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPN 120 LDLGDLNSQLKRLTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPN Sbjct: 61 LDLGDLNSQLKRLTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPN 120 Query: 121 AYTLLATRMLNATYCFQKALTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTNEPADENK 180 AYTLLATRMLNATYCFQKALTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTNEPADENK Sbjct: 121 AYTLLATRMLNATYCFQKALTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTNEPADENK 180 Query: 181 NQSIENADYSFSPEDFSVVRAFMQSLHSSKEAPDVK 216 NQSIENADYSFSPEDFSVVRAFMQSLHSSKEAPDVK Sbjct: 181 NQSIENADYSFSPEDFSVVRAFMQSLHSSKEAPDVK 216 >4H61-A nrPDB Length = 175 Score = 321 bits (822), Expect = 2e-88, Method: Composition-based stats. Identities = 171/172 (99%), Positives = 172/172 (100%) Query: 9 VDLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNALDLGDLNS 68 +DLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNALDLGDLNS Sbjct: 4 MDLTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQFNALDLGDLNS 63 Query: 69 QLKRLTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPNAYTLLATR 128 QLKRLTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPNAYTLLATR Sbjct: 64 QLKRLTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPNAYTLLATR 123 Query: 129 MLNATYCFQKALTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTNEPADENK 180 MLNATYCFQKALTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTNEPADENK Sbjct: 124 MLNATYCFQKALTKIEKFPQYNPQEGYTYPKLSNDNLEVDHSNTNEPADENK 175 >3RJ1-G nrPDB Length = 295 Score = 218 bits (555), Expect = 2e-57, Method: Composition-based stats. Identities = 59/198 (29%), Positives = 93/198 (46%), Gaps = 52/198 (26%) Query: 11 LTSIQWRMPEWVQSMGGLRTENVLEYFSQSPFYSHKSNNEMLKMQSQF------------ 58 L +QW+ PEW+Q G LRTENVL+YF++SPF+ SNN+++KMQ QF Sbjct: 6 LDELQWKSPEWIQVFG-LRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNAAVNM 64 Query: 59 --NALDLGD-----------------------------LNSQLKRLTGIQFVIIHERPPF 87 N + L D L +L +L G ++V+ R P Sbjct: 65 TQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSVREPD 124 Query: 88 LWVIQKQNRLNEN--------EVKPLTVYFVCNENIYMAPNAYTLLATRMLNATYCFQKA 139 WVI+KQ R N + E+ PL Y++ NIY +P + ++ +R+++ +Y Sbjct: 125 FWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYHLNST 184 Query: 140 LTKIEKFPQYNPQEGYTY 157 L + ++ P +G Y Sbjct: 185 LESLYDLIEFQPSQGVHY 202 >3QP5-A nrPDB Length = 265 Score = 32.4 bits (72), Expect = 0.17, Method: Composition-based stats. Identities = 19/78 (24%), Positives = 32/78 (41%), Gaps = 12/78 (15%) Query: 7 PSVDLTSIQWRMPEWVQSMGGLRTENVLEYF-----SQSP-------FYSHKSNNEMLKM 54 P+ S QW + EW+ G + TEN L+ F SQ+P + N++ ++ Sbjct: 13 PAGLTASQQWTLLEWIHMAGHIETENELKAFLDQVLSQAPSERLLLALGRLNNQNQIQRL 72 Query: 55 QSQFNALDLGDLNSQLKR 72 + N D Q + Sbjct: 73 ERVLNVSYPSDWLDQYMK 90 >1PD0-A nrPDB Length = 810 Score = 28.9 bits (63), Expect = 1.9, Method: Composition-based stats. Identities = 30/128 (23%), Positives = 47/128 (36%), Gaps = 31/128 (24%) Query: 94 QNRLNENEVKPLTVYFVCNENIYMAPNAYTLLATRMLNATYCF----------------- 136 ++R + NE+K C YMAP YTL ATYCF Sbjct: 159 KSRYDRNEIK-------CAVMEYMAPKEYTLRQPP--PATYCFLIDVSQSSIKSGLLATT 209 Query: 137 -QKALTKIEKFPQYNPQEGYTYPKLSND----NLEVDHSNTNEPADENKNQSIENADYSF 191 L ++ P ++ + + + N + +D N E AD+ I + + F Sbjct: 210 INTLLQNLDSIPNHDERTRISILCVDNAIHYFKIPLDSENNEESADQINMMDIADLEEPF 269 Query: 192 SPEDFSVV 199 P S+V Sbjct: 270 LPRPNSMV 277 >1M2V-B nrPDB Length = 926 Score = 28.1 bits (61), Expect = 2.9, Method: Composition-based stats. Identities = 30/128 (23%), Positives = 47/128 (36%), Gaps = 31/128 (24%) Query: 94 QNRLNENEVKPLTVYFVCNENIYMAPNAYTLLATRMLNATYCF----------------- 136 ++R + NE+K C YMAP YTL ATYCF Sbjct: 275 KSRYDRNEIK-------CAVMEYMAPKEYTLRQPP--PATYCFLIDVSQSSIKSGLLATT 325 Query: 137 -QKALTKIEKFPQYNPQEGYTYPKLSND----NLEVDHSNTNEPADENKNQSIENADYSF 191 L ++ P ++ + + + N + +D N E AD+ I + + F Sbjct: 326 INTLLQNLDSIPNHDERTRISILCVDNAIHYFKIPLDSENNEESADQINMMDIADLEEPF 385 Query: 192 SPEDFSVV 199 P S+V Sbjct: 386 LPRPNSMV 393 >1DPE-A nrPDB Length = 507 Score = 27.4 bits (59), Expect = 4.9, Method: Composition-based stats. Identities = 11/33 (33%), Positives = 19/33 (57%), Gaps = 2/33 (6%) Query: 58 FNALDLGDLNSQLKRLTG--IQFVIIHERPPFL 88 F + L +L S++K++ +QFV+ PFL Sbjct: 115 FEGMGLPELISEVKKVDDNTVQFVLTRPEAPFL 147 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.307 0.130 0.377 Lambda K H 0.267 0.0395 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,314,632 Number of Sequences: 36641 Number of extensions: 304728 Number of successful extensions: 748 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 732 Number of HSP's gapped (non-prelim): 22 length of query: 216 length of database: 10,336,785 effective HSP length: 93 effective length of query: 123 effective length of database: 6,929,172 effective search space: 852288156 effective search space used: 852288156 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.1 bits) S2: 57 (26.6 bits)