BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9UKL6 (214 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1LN1-A nrPDB 446 e-126 3BH2-A nrPDB 33 0.11 1O0W-A nrPDB 30 0.60 2Z87-A nrPDB 28 2.6 1D2I-A nrPDB 28 3.2 2FVO-A nrPDB 27 9.5 >1LN1-A nrPDB Length = 214 Score = 446 bits (1147), Expect = e-126, Method: Compositional matrix adjust. Identities = 214/214 (100%), Positives = 214/214 (100%) Query: 1 MELAAGSFSEEQFWEACAELQQPALAGADWQLLVETSGISIYRLLDKKTGLYEYKVFGVL 60 MELAAGSFSEEQFWEACAELQQPALAGADWQLLVETSGISIYRLLDKKTGLYEYKVFGVL Sbjct: 1 MELAAGSFSEEQFWEACAELQQPALAGADWQLLVETSGISIYRLLDKKTGLYEYKVFGVL 60 Query: 61 EDCSPTLLADIYMDSDYRKQWDQYVKELYEQECNGETVVYWEVKYPFPMSNRDYVYLRQR 120 EDCSPTLLADIYMDSDYRKQWDQYVKELYEQECNGETVVYWEVKYPFPMSNRDYVYLRQR Sbjct: 61 EDCSPTLLADIYMDSDYRKQWDQYVKELYEQECNGETVVYWEVKYPFPMSNRDYVYLRQR 120 Query: 121 RDLDMEGRKIHVILARSTSMPQLGERSGVIRVKQYKQSLAIESDGKKGSKVFMYYFDNPG 180 RDLDMEGRKIHVILARSTSMPQLGERSGVIRVKQYKQSLAIESDGKKGSKVFMYYFDNPG Sbjct: 121 RDLDMEGRKIHVILARSTSMPQLGERSGVIRVKQYKQSLAIESDGKKGSKVFMYYFDNPG 180 Query: 181 GQIPSWLINWAAKNGVPNFLKDMARACQNYLKKT 214 GQIPSWLINWAAKNGVPNFLKDMARACQNYLKKT Sbjct: 181 GQIPSWLINWAAKNGVPNFLKDMARACQNYLKKT 214 >3BH2-A nrPDB Length = 244 Score = 32.7 bits (73), Expect = 0.11, Method: Compositional matrix adjust. Identities = 28/104 (26%), Positives = 44/104 (42%), Gaps = 8/104 (7%) Query: 106 PFPMSNRDYVYLRQRRDLDM------EGRKIHVILARSTSMPQLGERSGVIRVKQYKQSL 159 P+ NR+Y + R D+D E +I L R M + + SG+ + Q++ Sbjct: 23 PYKFHNREYFNIVYRTDMDALRKVVPEPLEIDEPLVRFEIM-AMHDTSGLGCYTESGQAI 81 Query: 160 AIESDGKKGSKVFMYYFDN-PGGQIPSWLINWAAKNGVPNFLKD 202 + +G KG + M Y DN P + L + K G P D Sbjct: 82 PVSFNGVKGDYLHMMYLDNEPAIAVGRELSAYPKKLGYPKLFVD 125 >1O0W-A nrPDB Length = 252 Score = 30.4 bits (67), Expect = 0.60, Method: Compositional matrix adjust. Identities = 16/34 (47%), Positives = 20/34 (58%), Gaps = 5/34 (14%) Query: 59 VLEDCSPTLLADIYMDSDYRKQWDQYVKELYEQE 92 +L D LLA IY+D Y K +KEL+EQE Sbjct: 136 ILADAFEALLAAIYLDQGYEK-----IKELFEQE 164 >2Z87-A nrPDB Length = 624 Score = 28.1 bits (61), Expect = 2.6, Method: Compositional matrix adjust. Identities = 14/36 (38%), Positives = 20/36 (55%) Query: 106 PFPMSNRDYVYLRQRRDLDMEGRKIHVILARSTSMP 141 P P S DYV+ +R++LD RK +I S +P Sbjct: 64 PLPESTNDYVWAGKRKELDDYPRKQLIIDGLSIVIP 99 >1D2I-A nrPDB Length = 223 Score = 28.1 bits (61), Expect = 3.2, Method: Compositional matrix adjust. Identities = 12/39 (30%), Positives = 22/39 (56%), Gaps = 2/39 (5%) Query: 105 YPFPMSN--RDYVYLRQRRDLDMEGRKIHVILARSTSMP 141 YPF ++N R ++ + D+D EG K+ +I+ + P Sbjct: 99 YPFLLNNTVRSELFHKSNMDIDEEGMKVAIIITKGHMFP 137 >2FVO-A nrPDB Length = 333 Score = 26.6 bits (57), Expect = 9.5, Method: Compositional matrix adjust. Identities = 22/82 (26%), Positives = 39/82 (47%), Gaps = 3/82 (3%) Query: 78 RKQWDQYVKELYEQECNG--ETVVYWEVKYPFPMSNRDY-VYLRQRRDLDMEGRKIHVIL 134 RK W+ V E++E + G E V + + K + + + V+ QR + G +IH Sbjct: 118 RKGWNLEVAEIHETDLGGIREVVFFVKGKNAYGILKYESGVHRVQRVPVTESGGRIHTST 177 Query: 135 ARSTSMPQLGERSGVIRVKQYK 156 A +P++ E+ IR + K Sbjct: 178 ATVAVLPEIEEKDIEIRPEDLK 199 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1LN1-A nrPDB 468 e-133 Sequences not found previously or not previously below threshold: 3BH2-A nrPDB 33 0.081 1O0W-A nrPDB 32 0.27 2Z87-A nrPDB 29 1.2 2FVO-A nrPDB 29 1.6 1D2I-A nrPDB 28 2.1 5BRL-A nrPDB 28 3.0 4C9D-A nrPDB 28 3.2 6ENS-A nrPDB 28 3.6 1BEH-A nrPDB 28 3.6 1A44-A nrPDB 28 3.6 3O2K-A nrPDB 27 5.9 CONVERGED! >1LN1-A nrPDB Length = 214 Score = 468 bits (1205), Expect = e-133, Method: Composition-based stats. Identities = 214/214 (100%), Positives = 214/214 (100%) Query: 1 MELAAGSFSEEQFWEACAELQQPALAGADWQLLVETSGISIYRLLDKKTGLYEYKVFGVL 60 MELAAGSFSEEQFWEACAELQQPALAGADWQLLVETSGISIYRLLDKKTGLYEYKVFGVL Sbjct: 1 MELAAGSFSEEQFWEACAELQQPALAGADWQLLVETSGISIYRLLDKKTGLYEYKVFGVL 60 Query: 61 EDCSPTLLADIYMDSDYRKQWDQYVKELYEQECNGETVVYWEVKYPFPMSNRDYVYLRQR 120 EDCSPTLLADIYMDSDYRKQWDQYVKELYEQECNGETVVYWEVKYPFPMSNRDYVYLRQR Sbjct: 61 EDCSPTLLADIYMDSDYRKQWDQYVKELYEQECNGETVVYWEVKYPFPMSNRDYVYLRQR 120 Query: 121 RDLDMEGRKIHVILARSTSMPQLGERSGVIRVKQYKQSLAIESDGKKGSKVFMYYFDNPG 180 RDLDMEGRKIHVILARSTSMPQLGERSGVIRVKQYKQSLAIESDGKKGSKVFMYYFDNPG Sbjct: 121 RDLDMEGRKIHVILARSTSMPQLGERSGVIRVKQYKQSLAIESDGKKGSKVFMYYFDNPG 180 Query: 181 GQIPSWLINWAAKNGVPNFLKDMARACQNYLKKT 214 GQIPSWLINWAAKNGVPNFLKDMARACQNYLKKT Sbjct: 181 GQIPSWLINWAAKNGVPNFLKDMARACQNYLKKT 214 >3BH2-A nrPDB Length = 244 Score = 33.1 bits (74), Expect = 0.081, Method: Composition-based stats. Identities = 28/104 (26%), Positives = 44/104 (42%), Gaps = 8/104 (7%) Query: 106 PFPMSNRDYVYLRQRRDLDM------EGRKIHVILARSTSMPQLGERSGVIRVKQYKQSL 159 P+ NR+Y + R D+D E +I L R M + + SG+ + Q++ Sbjct: 23 PYKFHNREYFNIVYRTDMDALRKVVPEPLEIDEPLVRFEIMA-MHDTSGLGCYTESGQAI 81 Query: 160 AIESDGKKGSKVFMYYFDN-PGGQIPSWLINWAAKNGVPNFLKD 202 + +G KG + M Y DN P + L + K G P D Sbjct: 82 PVSFNGVKGDYLHMMYLDNEPAIAVGRELSAYPKKLGYPKLFVD 125 >1O0W-A nrPDB Length = 252 Score = 31.6 bits (70), Expect = 0.27, Method: Composition-based stats. Identities = 16/34 (47%), Positives = 20/34 (58%), Gaps = 5/34 (14%) Query: 59 VLEDCSPTLLADIYMDSDYRKQWDQYVKELYEQE 92 +L D LLA IY+D Y K +KEL+EQE Sbjct: 136 ILADAFEALLAAIYLDQGYEK-----IKELFEQE 164 >2Z87-A nrPDB Length = 624 Score = 29.2 bits (64), Expect = 1.2, Method: Composition-based stats. Identities = 14/36 (38%), Positives = 20/36 (55%) Query: 106 PFPMSNRDYVYLRQRRDLDMEGRKIHVILARSTSMP 141 P P S DYV+ +R++LD RK +I S +P Sbjct: 64 PLPESTNDYVWAGKRKELDDYPRKQLIIDGLSIVIP 99 >2FVO-A nrPDB Length = 333 Score = 28.9 bits (63), Expect = 1.6, Method: Composition-based stats. Identities = 22/82 (26%), Positives = 39/82 (47%), Gaps = 3/82 (3%) Query: 78 RKQWDQYVKELYEQECNG--ETVVYWEVKYPFPMSNRDY-VYLRQRRDLDMEGRKIHVIL 134 RK W+ V E++E + G E V + + K + + + V+ QR + G +IH Sbjct: 118 RKGWNLEVAEIHETDLGGIREVVFFVKGKNAYGILKYESGVHRVQRVPVTESGGRIHTST 177 Query: 135 ARSTSMPQLGERSGVIRVKQYK 156 A +P++ E+ IR + K Sbjct: 178 ATVAVLPEIEEKDIEIRPEDLK 199 >1D2I-A nrPDB Length = 223 Score = 28.5 bits (62), Expect = 2.1, Method: Composition-based stats. Identities = 12/39 (30%), Positives = 22/39 (56%), Gaps = 2/39 (5%) Query: 105 YPFPMSN--RDYVYLRQRRDLDMEGRKIHVILARSTSMP 141 YPF ++N R ++ + D+D EG K+ +I+ + P Sbjct: 99 YPFLLNNTVRSELFHKSNMDIDEEGMKVAIIITKGHMFP 137 >5BRL-A nrPDB Length = 210 Score = 28.1 bits (61), Expect = 3.0, Method: Composition-based stats. Identities = 16/79 (20%), Positives = 38/79 (48%), Gaps = 6/79 (7%) Query: 20 LQQPALAGADWQLLVETSGISIYRLLDKKTGLYEYKVFGVLEDCSPTLLADIYMDSDYRK 79 +Q ++ +W++ + ++++R ++ Y YK GV++D ++ D +R Sbjct: 23 IQYHSIKEDEWRVAKKVKDVTVWRKPSEEFNGYLYKAQGVMDDVVNNVI-DHIRPGPWRL 81 Query: 80 QWDQYVKEL-----YEQEC 93 WD+ + L +E+ C Sbjct: 82 DWDRLMTSLDVLEHFEENC 100 >4C9D-A nrPDB Length = 268 Score = 28.1 bits (61), Expect = 3.2, Method: Composition-based stats. Identities = 22/95 (23%), Positives = 40/95 (42%), Gaps = 10/95 (10%) Query: 95 GETVVYWEVKYPFPMSNRDYVYLRQRRDLDME-----GRKIHVILARSTSMPQLGERSGV 149 G + W+ PFP + ++ LR+ +LD+E + H + A P +G Sbjct: 170 GSLLDKWQAHSPFPYNPKEEAALRELFELDLEVAGFRNLRFHRVQAGKGFFPGF---TGE 226 Query: 150 IRVKQYKQSLAIESDGKKGSKVFMYYFDNPGGQIP 184 ++ + QSL E+ G + +F G + P Sbjct: 227 ATLRLWSQSL--EAQEALGRLHALAFFSGVGAKTP 259 >6ENS-A nrPDB Length = 193 Score = 27.7 bits (60), Expect = 3.6, Method: Composition-based stats. Identities = 9/15 (60%), Positives = 12/15 (80%) Query: 77 YRKQWDQYVKELYEQ 91 Y+ +WD YV +LYEQ Sbjct: 175 YQAEWDDYVPKLYEQ 189 >1BEH-A nrPDB Length = 187 Score = 27.7 bits (60), Expect = 3.6, Method: Composition-based stats. Identities = 9/15 (60%), Positives = 12/15 (80%) Query: 77 YRKQWDQYVKELYEQ 91 Y+ +WD YV +LYEQ Sbjct: 169 YQAEWDDYVPKLYEQ 183 >1A44-A nrPDB Length = 185 Score = 27.7 bits (60), Expect = 3.6, Method: Composition-based stats. Identities = 9/15 (60%), Positives = 12/15 (80%) Query: 77 YRKQWDQYVKELYEQ 91 Y+ +WD YV +LYEQ Sbjct: 168 YQAEWDDYVPKLYEQ 182 >3O2K-A nrPDB Length = 474 Score = 26.9 bits (58), Expect = 5.9, Method: Composition-based stats. Identities = 12/40 (30%), Positives = 23/40 (57%) Query: 6 GSFSEEQFWEACAELQQPALAGADWQLLVETSGISIYRLL 45 GS EE++W + A + L +++ + V+ +S+YR L Sbjct: 29 GSVDEERWWHSTAPILSRLLISSNYDVDVQYKYLSLYRHL 68 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.319 0.135 0.417 Lambda K H 0.267 0.0416 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,584,433 Number of Sequences: 36641 Number of extensions: 415711 Number of successful extensions: 954 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 940 Number of HSP's gapped (non-prelim): 26 length of query: 214 length of database: 10,336,785 effective HSP length: 93 effective length of query: 121 effective length of database: 6,929,172 effective search space: 838429812 effective search space used: 838429812 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 57 (26.5 bits)