BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9SWE5 (209 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1MVN-A nrPDB 432 e-122 1QZU-A nrPDB 181 1e-46 5H75-A nrPDB 68 3e-12 1P3Y-1 nrPDB 68 3e-12 4ZAN-A nrPDB 37 0.008 1D6Z-A nrPDB 32 0.13 1SBZ-A nrPDB 29 1.3 3BP8-A nrPDB 29 1.5 3MCU-A nrPDB 28 2.6 4AR9-A nrPDB 27 7.5 >1MVN-A nrPDB Length = 209 Score = 432 bits (1111), Expect = e-122, Method: Compositional matrix adjust. Identities = 208/209 (99%), Positives = 208/209 (99%) Query: 1 MENGKRDRQDMEVNTTPRKPRVLLAASGSVAAIKFGNLCHCFTEWAEVRAVVTKSSLHFL 60 MENGKRDRQDMEVNTTPRKPRVLLAASGSVAAIKFGNLCHCFTEWAEVRAVVTKSSLHFL Sbjct: 1 MENGKRDRQDMEVNTTPRKPRVLLAASGSVAAIKFGNLCHCFTEWAEVRAVVTKSSLHFL 60 Query: 61 DKLSLPQEVTLYTDEDEWSSWNKIGDPVLHIELRRWADVLVIAPLSANTLGKIAGGLCDN 120 DKLSLPQEVTLYTDEDEWSSWNKIGDPVLHIELRRWADVLVIAPLSANTLGKIAGGLCDN Sbjct: 61 DKLSLPQEVTLYTDEDEWSSWNKIGDPVLHIELRRWADVLVIAPLSANTLGKIAGGLCDN 120 Query: 121 LLTCIIRAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLACGDYGN 180 LLTCIIRAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLA GDYGN Sbjct: 121 LLTCIIRAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLASGDYGN 180 Query: 181 GAMAEPSLIYSTVRLFWESQAHQQTGGTS 209 GAMAEPSLIYSTVRLFWESQAHQQTGGTS Sbjct: 181 GAMAEPSLIYSTVRLFWESQAHQQTGGTS 209 >1QZU-A nrPDB Length = 206 Score = 181 bits (460), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 93/191 (48%), Positives = 120/191 (62%), Gaps = 5/191 (2%) Query: 18 RKPRVLLAASGSVAAIKFGNLCHCFTEWA--EVRAVVTKSSLHFLDKLSLPQEVTLYTDE 75 RK VL+ +GSVAA+K L + EV V T+ + HF +P VTLY+D Sbjct: 18 RKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFYSPQDIP--VTLYSDA 75 Query: 76 DEWSSWNKIGDPVLHIELRRWADVLVIAPLSANTLGKIAGGLCDNLLTCIIRAWDYTKPL 135 DEW W DPVLHI+LRRWAD+L++APL ANTLGK+A G+CDNLLTC++RAWD +KPL Sbjct: 76 DEWEMWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLLTCVMRAWDRSKPL 135 Query: 136 FVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLACGDYGNGAMAEPSLIYSTVR- 194 PAMNT MW +P T + + L G IP + K+L CGD G GAMAE I V+ Sbjct: 136 LFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGAMAEVGTIVDKVKE 195 Query: 195 LFWESQAHQQT 205 + ++ QQ+ Sbjct: 196 VLFQHSGFQQS 206 >5H75-A nrPDB Length = 238 Score = 68.2 bits (165), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 39/126 (30%), Positives = 63/126 (50%), Gaps = 7/126 (5%) Query: 45 WAEVRAVVTKSSLHFLDKLSLPQEVTLYTDEDEWSSWNKIGDPVLHIELRRWADVLVIAP 104 + E+R V+TK++ + P Y + +S + G H+E+ RWAD+ I P Sbjct: 42 FKEIRVVMTKTAEDLI-----PAHTVSYFCDHVYSEHGENGKRHSHVEIGRWADIYCIIP 96 Query: 105 LSANTLGKIAGGLCDNLLTCIIRAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGIT 164 +AN LG+ A G+ NL+ + A + F P MN LMWN R++ L + G Sbjct: 97 ATANILGQTANGVAMNLVATTVLAHPHNTIFF--PNMNDLMWNKTVVSRNIEQLRKDGHI 154 Query: 165 LIPPIK 170 +I P++ Sbjct: 155 VIEPVE 160 >1P3Y-1 nrPDB Length = 194 Score = 67.8 bits (164), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 39/126 (30%), Positives = 63/126 (50%), Gaps = 7/126 (5%) Query: 45 WAEVRAVVTKSSLHFLDKLSLPQEVTLYTDEDEWSSWNKIGDPVLHIELRRWADVLVIAP 104 + E+R V+TK++ + P Y + +S + G H+E+ RWAD+ I P Sbjct: 35 FKEIRVVMTKTAEDLI-----PAHTVSYFCDHVYSEHGENGKRHSHVEIGRWADIYCIIP 89 Query: 105 LSANTLGKIAGGLCDNLLTCIIRAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGIT 164 +AN LG+ A G+ NL+ + A + F P MN LMWN R++ L + G Sbjct: 90 ATANILGQTANGVAMNLVATTVLAHPHNTIFF--PNMNDLMWNKTVVSRNIEQLRKDGHI 147 Query: 165 LIPPIK 170 +I P++ Sbjct: 148 VIEPVE 153 >4ZAN-A nrPDB Length = 209 Score = 36.6 bits (83), Expect = 0.008, Method: Compositional matrix adjust. Identities = 27/77 (35%), Positives = 36/77 (46%), Gaps = 16/77 (20%) Query: 98 DVLVIAPLSANTLGKIAGGLCDNLLTCIIRAWDYT----KPLFVAPAMNTLMWNNPFTER 153 + +VI P S TL +A G C+NL I RA D +PL + P PF+ Sbjct: 96 NAMVICPCSTGTLSAVATGACNNL---IERAADVALKERRPLVLVP------REAPFSSI 146 Query: 154 HL---LSLDELGITLIP 167 HL L L LG ++P Sbjct: 147 HLENMLKLSNLGAVILP 163 >1D6Z-A nrPDB Length = 727 Score = 32.3 bits (72), Expect = 0.13, Method: Compositional matrix adjust. Identities = 20/70 (28%), Positives = 33/70 (47%), Gaps = 10/70 (14%) Query: 127 RAWDYTKPL--FVAPAMNTLMWNNPFTERHLLSLDELGITLIP--------PIKKRLACG 176 R WD+ + V P ++T+ +N+ T+R ++ LG ++P K L G Sbjct: 326 RNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSLGGMIVPYGDPDIGWYFKAYLDSG 385 Query: 177 DYGNGAMAEP 186 DYG G + P Sbjct: 386 DYGMGTLTSP 395 >1SBZ-A nrPDB Length = 197 Score = 29.3 bits (64), Expect = 1.3, Method: Compositional matrix adjust. Identities = 22/79 (27%), Positives = 35/79 (44%), Gaps = 16/79 (20%) Query: 98 DVLVIAPLSANTLGKIAGGLCDNLLTCIIRAWDYT----KPLFVAPAMNTLMWNNPFTER 153 D +++ P S TL I G D L+ RA D + L + P P + Sbjct: 79 DGMIVIPCSMKTLAGIRAGYADGLVG---RAADVVLKEGRKLVLVP------REMPLSTI 129 Query: 154 HL---LSLDELGITLIPPI 169 HL L+L +G+ ++PP+ Sbjct: 130 HLENMLALSRMGVAMVPPM 148 >3BP8-A nrPDB Length = 406 Score = 28.9 bits (63), Expect = 1.5, Method: Compositional matrix adjust. Identities = 22/68 (32%), Positives = 32/68 (47%), Gaps = 14/68 (20%) Query: 126 IRAWDYTKPLFVAP-----AMNTLMWNNP----FTERHLL-----SLDELGITLIPPIKK 171 I AW + LF A + ++ +N T+ HLL SL E+G T + P K Sbjct: 196 ISAWTMAEALFGASRGARDVIQVVIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGK 255 Query: 172 RLACGDYG 179 R CG++G Sbjct: 256 RCYCGNHG 263 >3MCU-A nrPDB Length = 207 Score = 28.1 bits (61), Expect = 2.6, Method: Compositional matrix adjust. Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%) Query: 98 DVLVIAPLSANTLGKIAGGLCDNLLTCIIRA-WDYTKPLFVAPAMN 142 D +VIAPL+ N++ K A + D+ + +A KP+ +A + N Sbjct: 86 DCMVIAPLTGNSMSKFANAMTDSPVLMAAKATLRNGKPVVLAVSTN 131 >4AR9-A nrPDB Length = 394 Score = 26.6 bits (57), Expect = 7.5, Method: Compositional matrix adjust. Identities = 11/37 (29%), Positives = 21/37 (56%) Query: 52 VTKSSLHFLDKLSLPQEVTLYTDEDEWSSWNKIGDPV 88 +TK S + D +L TL +++ E S+WN + + + Sbjct: 312 ITKESSQYFDTYNLKATYTLSSNKGEISNWNYMNNKI 348 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1MVN-A nrPDB 380 e-106 1QZU-A nrPDB 290 3e-79 5H75-A nrPDB 184 2e-47 1P3Y-1 nrPDB 184 2e-47 Sequences not found previously or not previously below threshold: 4ZAN-A nrPDB 32 0.17 6NCL-a0 nrPDB 31 0.39 3BDR-A nrPDB 29 1.2 6GAW-Bm nrPDB 29 2.0 1Z05-A nrPDB 29 2.4 3BP8-A nrPDB 28 3.9 1D6Z-A nrPDB 27 4.6 6HU9-k nrPDB 27 5.7 CONVERGED! >1MVN-A nrPDB Length = 209 Score = 380 bits (976), Expect = e-106, Method: Composition-based stats. Identities = 208/209 (99%), Positives = 208/209 (99%) Query: 1 MENGKRDRQDMEVNTTPRKPRVLLAASGSVAAIKFGNLCHCFTEWAEVRAVVTKSSLHFL 60 MENGKRDRQDMEVNTTPRKPRVLLAASGSVAAIKFGNLCHCFTEWAEVRAVVTKSSLHFL Sbjct: 1 MENGKRDRQDMEVNTTPRKPRVLLAASGSVAAIKFGNLCHCFTEWAEVRAVVTKSSLHFL 60 Query: 61 DKLSLPQEVTLYTDEDEWSSWNKIGDPVLHIELRRWADVLVIAPLSANTLGKIAGGLCDN 120 DKLSLPQEVTLYTDEDEWSSWNKIGDPVLHIELRRWADVLVIAPLSANTLGKIAGGLCDN Sbjct: 61 DKLSLPQEVTLYTDEDEWSSWNKIGDPVLHIELRRWADVLVIAPLSANTLGKIAGGLCDN 120 Query: 121 LLTCIIRAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLACGDYGN 180 LLTCIIRAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLA GDYGN Sbjct: 121 LLTCIIRAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLASGDYGN 180 Query: 181 GAMAEPSLIYSTVRLFWESQAHQQTGGTS 209 GAMAEPSLIYSTVRLFWESQAHQQTGGTS Sbjct: 181 GAMAEPSLIYSTVRLFWESQAHQQTGGTS 209 >1QZU-A nrPDB Length = 206 Score = 290 bits (742), Expect = 3e-79, Method: Composition-based stats. Identities = 93/191 (48%), Positives = 120/191 (62%), Gaps = 5/191 (2%) Query: 18 RKPRVLLAASGSVAAIKFGNLCHCFTEWA--EVRAVVTKSSLHFLDKLSLPQEVTLYTDE 75 RK VL+ +GSVAA+K L + EV V T+ + HF +P VTLY+D Sbjct: 18 RKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFYSPQDIP--VTLYSDA 75 Query: 76 DEWSSWNKIGDPVLHIELRRWADVLVIAPLSANTLGKIAGGLCDNLLTCIIRAWDYTKPL 135 DEW W DPVLHI+LRRWAD+L++APL ANTLGK+A G+CDNLLTC++RAWD +KPL Sbjct: 76 DEWEMWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLLTCVMRAWDRSKPL 135 Query: 136 FVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLACGDYGNGAMAEPSLIYSTVR- 194 PAMNT MW +P T + + L G IP + K+L CGD G GAMAE I V+ Sbjct: 136 LFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGAMAEVGTIVDKVKE 195 Query: 195 LFWESQAHQQT 205 + ++ QQ+ Sbjct: 196 VLFQHSGFQQS 206 >5H75-A nrPDB Length = 238 Score = 184 bits (468), Expect = 2e-47, Method: Composition-based stats. Identities = 39/126 (30%), Positives = 63/126 (50%), Gaps = 7/126 (5%) Query: 45 WAEVRAVVTKSSLHFLDKLSLPQEVTLYTDEDEWSSWNKIGDPVLHIELRRWADVLVIAP 104 + E+R V+TK++ + P Y + +S + G H+E+ RWAD+ I P Sbjct: 42 FKEIRVVMTKTAEDLI-----PAHTVSYFCDHVYSEHGENGKRHSHVEIGRWADIYCIIP 96 Query: 105 LSANTLGKIAGGLCDNLLTCIIRAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGIT 164 +AN LG+ A G+ NL+ + A + F P MN LMWN R++ L + G Sbjct: 97 ATANILGQTANGVAMNLVATTVLAHPHNTIFF--PNMNDLMWNKTVVSRNIEQLRKDGHI 154 Query: 165 LIPPIK 170 +I P++ Sbjct: 155 VIEPVE 160 >1P3Y-1 nrPDB Length = 194 Score = 184 bits (468), Expect = 2e-47, Method: Composition-based stats. Identities = 39/126 (30%), Positives = 63/126 (50%), Gaps = 7/126 (5%) Query: 45 WAEVRAVVTKSSLHFLDKLSLPQEVTLYTDEDEWSSWNKIGDPVLHIELRRWADVLVIAP 104 + E+R V+TK++ + P Y + +S + G H+E+ RWAD+ I P Sbjct: 35 FKEIRVVMTKTAEDLI-----PAHTVSYFCDHVYSEHGENGKRHSHVEIGRWADIYCIIP 89 Query: 105 LSANTLGKIAGGLCDNLLTCIIRAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGIT 164 +AN LG+ A G+ NL+ + A + F P MN LMWN R++ L + G Sbjct: 90 ATANILGQTANGVAMNLVATTVLAHPHNTIFF--PNMNDLMWNKTVVSRNIEQLRKDGHI 147 Query: 165 LIPPIK 170 +I P++ Sbjct: 148 VIEPVE 153 >4ZAN-A nrPDB Length = 209 Score = 32.0 bits (71), Expect = 0.17, Method: Composition-based stats. Identities = 23/74 (31%), Positives = 33/74 (44%), Gaps = 10/74 (13%) Query: 98 DVLVIAPLSANTLGKIAGGLCDNLLTCII-RAWDYTKPLFVAPAMNTLMWNNPFTERHLL 156 + +VI P S TL +A G C+NL+ A +PL + P PF+ HL Sbjct: 96 NAMVICPCSTGTLSAVATGACNNLIERAADVALKERRPLVLVPR------EAPFSSIHLE 149 Query: 157 ---SLDELGITLIP 167 L LG ++P Sbjct: 150 NMLKLSNLGAVILP 163 >6NCL-a0 nrPDB Length = 352 Score = 30.8 bits (68), Expect = 0.39, Method: Composition-based stats. Identities = 15/92 (16%), Positives = 33/92 (35%), Gaps = 6/92 (6%) Query: 112 KIAGGLCDNLLTCIIRAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKK 171 K+ + DN + ++ +A + ++ +N + L + Sbjct: 18 KMTDAISDNQIIAT------SRFFNIARDVADVVVSNTKLAQQYEQLSIDSLKEYLVSVA 71 Query: 172 RLACGDYGNGAMAEPSLIYSTVRLFWESQAHQ 203 + DY N A+ + +RLF E + +Q Sbjct: 72 KFVAVDYSNTTSADVDDLIHKLRLFIEEECYQ 103 >3BDR-A nrPDB Length = 190 Score = 29.3 bits (64), Expect = 1.2, Method: Composition-based stats. Identities = 14/43 (32%), Positives = 20/43 (46%), Gaps = 1/43 (2%) Query: 158 LDELGITLIPPIKKRLACGDYG-NGAMAEPSLIYSTVRLFWES 199 L E+G P+ R + G G + E IYS RL++ S Sbjct: 105 LREMGYAEKAPVAGRFSMGSDGALTLITEYETIYSEERLWFAS 147 >6GAW-Bm nrPDB Length = 159 Score = 28.5 bits (62), Expect = 2.0, Method: Composition-based stats. Identities = 13/58 (22%), Positives = 21/58 (36%), Gaps = 5/58 (8%) Query: 115 GGLCDNLLTCIIRAWDYTKPLFVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKR 172 GL LTC + + F +P P + + + I + PPI+ R Sbjct: 7 SGLARRSLTCAVSGAPRRE--FWSPFRKE---KQPVVAETVEEVKKEPILVCPPIQSR 59 >1Z05-A nrPDB Length = 429 Score = 28.5 bits (62), Expect = 2.4, Method: Composition-based stats. Identities = 14/35 (40%), Positives = 16/35 (45%), Gaps = 1/35 (2%) Query: 160 ELGITLIPPIKKRLACGDYG-NGAMAEPSLIYSTV 193 ELG I P KR CG+YG +A I V Sbjct: 266 ELGHIQIDPQGKRCHCGNYGCLETVASSQAIRDQV 300 >3BP8-A nrPDB Length = 406 Score = 27.7 bits (60), Expect = 3.9, Method: Composition-based stats. Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 1/40 (2%) Query: 157 SLDELGITLIPPIKKRLACGDYG-NGAMAEPSLIYSTVRL 195 SL E+G T + P KR CG++G +A I +L Sbjct: 241 SLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILELAQL 280 >1D6Z-A nrPDB Length = 727 Score = 27.4 bits (59), Expect = 4.6, Method: Composition-based stats. Identities = 20/70 (28%), Positives = 33/70 (47%), Gaps = 10/70 (14%) Query: 127 RAWDYTKPL--FVAPAMNTLMWNNPFTERHLLSLDELGITLIPP--------IKKRLACG 176 R WD+ + V P ++T+ +N+ T+R ++ LG ++P K L G Sbjct: 326 RNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSLGGMIVPYGDPDIGWYFKAYLDSG 385 Query: 177 DYGNGAMAEP 186 DYG G + P Sbjct: 386 DYGMGTLTSP 395 >6HU9-k nrPDB Length = 131 Score = 27.0 bits (58), Expect = 5.7, Method: Composition-based stats. Identities = 10/26 (38%), Positives = 12/26 (46%), Gaps = 1/26 (3%) Query: 131 YTKPLFVAPAMNTLMWNNPFTERHLL 156 +KP F TL WN P RH+ Sbjct: 93 RSKPFFWGDGDKTLFWN-PVVNRHIE 117 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.313 0.132 0.376 Lambda K H 0.267 0.0403 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,759,151 Number of Sequences: 36641 Number of extensions: 331845 Number of successful extensions: 603 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 15 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 577 Number of HSP's gapped (non-prelim): 23 length of query: 209 length of database: 10,336,785 effective HSP length: 93 effective length of query: 116 effective length of database: 6,929,172 effective search space: 803783952 effective search space used: 803783952 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.4 bits) S2: 57 (26.6 bits)