BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9SIY3 (210 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5A5Y-A nrPDB 431 e-121 1H80-A nrPDB 31 0.49 4YDO-A nrPDB 27 5.2 >5A5Y-A nrPDB Length = 212 Score = 431 bits (1107), Expect = e-121, Method: Compositional matrix adjust. Identities = 210/210 (100%), Positives = 210/210 (100%) Query: 1 MEVEVKLRLLTAAAHLRLTTLLTPYHLKTLHQRNTFFDTPKNDLSLRRAVLRLRFLQNAA 60 MEVEVKLRLLTAAAHLRLTTLLTPYHLKTLHQRNTFFDTPKNDLSLRRAVLRLRFLQNAA Sbjct: 3 MEVEVKLRLLTAAAHLRLTTLLTPYHLKTLHQRNTFFDTPKNDLSLRRAVLRLRFLQNAA 62 Query: 61 VSAASPSPPRCIVSLKAKPTLANGISRVEEDEEEIEYWIGKECVESPAKLSDIGSRVLKR 120 VSAASPSPPRCIVSLKAKPTLANGISRVEEDEEEIEYWIGKECVESPAKLSDIGSRVLKR Sbjct: 63 VSAASPSPPRCIVSLKAKPTLANGISRVEEDEEEIEYWIGKECVESPAKLSDIGSRVLKR 122 Query: 121 VKEEYGFNDFLGFVCLGGFENVRNVYEWRGVKLEVDETKYDFGNCYEIECETEEPERVKT 180 VKEEYGFNDFLGFVCLGGFENVRNVYEWRGVKLEVDETKYDFGNCYEIECETEEPERVKT Sbjct: 123 VKEEYGFNDFLGFVCLGGFENVRNVYEWRGVKLEVDETKYDFGNCYEIECETEEPERVKT 182 Query: 181 MIEEFLTEEKIEFSNSDMTKFAVFRSGKLP 210 MIEEFLTEEKIEFSNSDMTKFAVFRSGKLP Sbjct: 183 MIEEFLTEEKIEFSNSDMTKFAVFRSGKLP 212 >1H80-A nrPDB Length = 464 Score = 30.8 bits (68), Expect = 0.49, Method: Compositional matrix adjust. Identities = 20/98 (20%), Positives = 41/98 (41%), Gaps = 6/98 (6%) Query: 33 RNTFFDTPKNDLSLRRAVLRLRFLQNAAVSAASPSPPRCIVSLKAKPTLANGISRVEED- 91 RN F D + L + F++N V + S C ++++ S +E Sbjct: 233 RNIFADNIRCSKGLAAVMFGPHFMKNGDVQVTNVSSVSCGSAVRSDSGFVELFSPTDEVH 292 Query: 92 -----EEEIEYWIGKECVESPAKLSDIGSRVLKRVKEE 124 ++ +E +G+ C ++P + G+R RV ++ Sbjct: 293 TRQSWKQAVESKLGRGCAQTPYARGNGGTRWAARVTQK 330 >4YDO-A nrPDB Length = 329 Score = 27.3 bits (59), Expect = 5.2, Method: Compositional matrix adjust. Identities = 11/37 (29%), Positives = 23/37 (62%), Gaps = 2/37 (5%) Query: 150 GVKLEVD--ETKYDFGNCYEIECETEEPERVKTMIEE 184 +L+VD ++KYD+ + ++ TEEP+ + + +E Sbjct: 18 SARLQVDMTDSKYDYSDITPVDINTEEPQICQILYDE 54 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5A5Y-A nrPDB 447 e-126 Sequences not found previously or not previously below threshold: 2V2V-A nrPDB 29 1.5 1H80-A nrPDB 29 1.7 1QHB-A nrPDB 28 2.4 4YDO-A nrPDB 28 3.7 2MBG-A nrPDB 27 4.8 6IW1-A nrPDB 27 7.7 5UBM-B nrPDB 27 8.1 4LOT-A nrPDB 27 8.1 4LOS-A nrPDB 27 8.1 4J1Y-A nrPDB 27 8.1 CONVERGED! >5A5Y-A nrPDB Length = 212 Score = 447 bits (1150), Expect = e-126, Method: Composition-based stats. Identities = 210/210 (100%), Positives = 210/210 (100%) Query: 1 MEVEVKLRLLTAAAHLRLTTLLTPYHLKTLHQRNTFFDTPKNDLSLRRAVLRLRFLQNAA 60 MEVEVKLRLLTAAAHLRLTTLLTPYHLKTLHQRNTFFDTPKNDLSLRRAVLRLRFLQNAA Sbjct: 3 MEVEVKLRLLTAAAHLRLTTLLTPYHLKTLHQRNTFFDTPKNDLSLRRAVLRLRFLQNAA 62 Query: 61 VSAASPSPPRCIVSLKAKPTLANGISRVEEDEEEIEYWIGKECVESPAKLSDIGSRVLKR 120 VSAASPSPPRCIVSLKAKPTLANGISRVEEDEEEIEYWIGKECVESPAKLSDIGSRVLKR Sbjct: 63 VSAASPSPPRCIVSLKAKPTLANGISRVEEDEEEIEYWIGKECVESPAKLSDIGSRVLKR 122 Query: 121 VKEEYGFNDFLGFVCLGGFENVRNVYEWRGVKLEVDETKYDFGNCYEIECETEEPERVKT 180 VKEEYGFNDFLGFVCLGGFENVRNVYEWRGVKLEVDETKYDFGNCYEIECETEEPERVKT Sbjct: 123 VKEEYGFNDFLGFVCLGGFENVRNVYEWRGVKLEVDETKYDFGNCYEIECETEEPERVKT 182 Query: 181 MIEEFLTEEKIEFSNSDMTKFAVFRSGKLP 210 MIEEFLTEEKIEFSNSDMTKFAVFRSGKLP Sbjct: 183 MIEEFLTEEKIEFSNSDMTKFAVFRSGKLP 212 >2V2V-A nrPDB Length = 271 Score = 28.9 bits (63), Expect = 1.5, Method: Composition-based stats. Identities = 16/45 (35%), Positives = 22/45 (48%) Query: 89 EEDEEEIEYWIGKECVESPAKLSDIGSRVLKRVKEEYGFNDFLGF 133 E EE+I+ I E E L DI + + E Y F ++LGF Sbjct: 190 EYAEEKIQRIISGEVEEIENVLGDIARELYPEINEVYRFVEYLGF 234 >1H80-A nrPDB Length = 464 Score = 28.9 bits (63), Expect = 1.7, Method: Composition-based stats. Identities = 20/98 (20%), Positives = 41/98 (41%), Gaps = 6/98 (6%) Query: 33 RNTFFDTPKNDLSLRRAVLRLRFLQNAAVSAASPSPPRCIVSLKAKPTLANGISRVEED- 91 RN F D + L + F++N V + S C ++++ S +E Sbjct: 233 RNIFADNIRCSKGLAAVMFGPHFMKNGDVQVTNVSSVSCGSAVRSDSGFVELFSPTDEVH 292 Query: 92 -----EEEIEYWIGKECVESPAKLSDIGSRVLKRVKEE 124 ++ +E +G+ C ++P + G+R RV ++ Sbjct: 293 TRQSWKQAVESKLGRGCAQTPYARGNGGTRWAARVTQK 330 >1QHB-A nrPDB Length = 596 Score = 28.5 bits (62), Expect = 2.4, Method: Composition-based stats. Identities = 16/47 (34%), Positives = 23/47 (48%) Query: 37 FDTPKNDLSLRRAVLRLRFLQNAAVSAASPSPPRCIVSLKAKPTLAN 83 FD+PKN +R A+ RL L+ A PP I + + T+ N Sbjct: 176 FDSPKNAAFIRSAIERLNGLEWFNTPAKLGDPPAEIRRRRGEVTVGN 222 >4YDO-A nrPDB Length = 329 Score = 27.7 bits (60), Expect = 3.7, Method: Composition-based stats. Identities = 11/35 (31%), Positives = 23/35 (65%), Gaps = 2/35 (5%) Query: 152 KLEVD--ETKYDFGNCYEIECETEEPERVKTMIEE 184 +L+VD ++KYD+ + ++ TEEP+ + + +E Sbjct: 20 RLQVDMTDSKYDYSDITPVDINTEEPQICQILYDE 54 >2MBG-A nrPDB Length = 265 Score = 27.3 bits (59), Expect = 4.8, Method: Composition-based stats. Identities = 12/44 (27%), Positives = 23/44 (52%) Query: 145 VYEWRGVKLEVDETKYDFGNCYEIECETEEPERVKTMIEEFLTE 188 +Y G+K +VDE K + E EP V ++++++L + Sbjct: 49 IYRVSGIKSKVDELKAAYDREESTNLEDYEPNTVASLLKQYLRD 92 >6IW1-A nrPDB Length = 395 Score = 26.5 bits (57), Expect = 7.7, Method: Composition-based stats. Identities = 31/100 (31%), Positives = 45/100 (45%), Gaps = 18/100 (18%) Query: 77 AKPTLANGISRVEEDEEEIEYWIGKECVESPAKLSDIGSRVLKRVKEEYGFNDF--LGFV 134 AK T A +S E D+ +I+Y I + +G+ K+E D L F Sbjct: 117 AKFTCAKSMSLFEVDQTKIQYVIRAQL--------HVGA------KQENWNTDIKTLKFD 162 Query: 135 CLGGFENVRNV-YEWRGVKLEVDETKYDFGNCYEIECETE 173 L G + V + Y ++ +V +T DFGN Y E ETE Sbjct: 163 ALSGSQEVEFIGYGKATLECQV-QTAVDFGNSYIAEMETE 201 >5UBM-B nrPDB Length = 152 Score = 26.5 bits (57), Expect = 8.1, Method: Composition-based stats. Identities = 13/36 (36%), Positives = 17/36 (47%) Query: 107 PAKLSDIGSRVLKRVKEEYGFNDFLGFVCLGGFENV 142 P D + V + K +Y F D + CL GFE V Sbjct: 9 PCPKEDTPNSVWEPAKAKYVFRDVVQITCLDGFEVV 44 >4LOT-A nrPDB Length = 249 Score = 26.5 bits (57), Expect = 8.1, Method: Composition-based stats. Identities = 13/36 (36%), Positives = 17/36 (47%) Query: 107 PAKLSDIGSRVLKRVKEEYGFNDFLGFVCLGGFENV 142 P D + V + K +Y F D + CL GFE V Sbjct: 119 PCPKEDTPNSVWEPAKAKYVFRDVVQITCLDGFEVV 154 >4LOS-A nrPDB Length = 187 Score = 26.5 bits (57), Expect = 8.1, Method: Composition-based stats. Identities = 13/36 (36%), Positives = 17/36 (47%) Query: 107 PAKLSDIGSRVLKRVKEEYGFNDFLGFVCLGGFENV 142 P D + V + K +Y F D + CL GFE V Sbjct: 122 PCPKEDTPNSVWEPAKAKYVFRDVVQITCLDGFEVV 157 >4J1Y-A nrPDB Length = 397 Score = 26.5 bits (57), Expect = 8.1, Method: Composition-based stats. Identities = 13/36 (36%), Positives = 17/36 (47%) Query: 107 PAKLSDIGSRVLKRVKEEYGFNDFLGFVCLGGFENV 142 P D + V + K +Y F D + CL GFE V Sbjct: 2 PCPKEDTPNSVWEPAKAKYVFRDVVQITCLDGFEVV 37 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.136 0.395 Lambda K H 0.267 0.0417 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,438,308 Number of Sequences: 36641 Number of extensions: 343884 Number of successful extensions: 938 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 22 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 914 Number of HSP's gapped (non-prelim): 38 length of query: 210 length of database: 10,336,785 effective HSP length: 93 effective length of query: 117 effective length of database: 6,929,172 effective search space: 810713124 effective search space used: 810713124 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 57 (26.5 bits)