BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9SF21 (200 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5V5F-A nrPDB 411 e-116 2Q3S-A nrPDB 289 7e-79 5HUS-A nrPDB 30 0.48 5B8F-A nrPDB 29 1.3 3QDK-A nrPDB 28 3.8 3MQG-A nrPDB 27 4.3 >5V5F-A nrPDB Length = 200 Score = 411 bits (1057), Expect = e-116, Method: Compositional matrix adjust. Identities = 200/200 (100%), Positives = 200/200 (100%) Query: 1 MASFDGQGFMMVDNSWVQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFDVYWDVKSV 60 MASFDGQGFMMVDNSWVQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFDVYWDVKSV Sbjct: 1 MASFDGQGFMMVDNSWVQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFDVYWDVKSV 60 Query: 61 STKSEWRLCSVKFSTKNFCLFLRLPNPFCDNLKDLYRFFASKFVTFVGVQIQEDLALLKE 120 STKSEWRLCSVKFSTKNFCLFLRLPNPFCDNLKDLYRFFASKFVTFVGVQIQEDLALLKE Sbjct: 61 STKSEWRLCSVKFSTKNFCLFLRLPNPFCDNLKDLYRFFASKFVTFVGVQIQEDLALLKE 120 Query: 121 NHGIVIRSSLEIGKLAAKARGTPIVEFLGTRELAHKILWYDMSRLDSIQSKWDEASSNDR 180 NHGIVIRSSLEIGKLAAKARGTPIVEFLGTRELAHKILWYDMSRLDSIQSKWDEASSNDR Sbjct: 121 NHGIVIRSSLEIGKLAAKARGTPIVEFLGTRELAHKILWYDMSRLDSIQSKWDEASSNDR 180 Query: 181 LEAAAIEGWLIFNVYDQLQQ 200 LEAAAIEGWLIFNVYDQLQQ Sbjct: 181 LEAAAIEGWLIFNVYDQLQQ 200 >2Q3S-A nrPDB Length = 206 Score = 289 bits (739), Expect = 7e-79, Method: Compositional matrix adjust. Identities = 137/202 (67%), Positives = 168/202 (83%), Gaps = 5/202 (2%) Query: 2 ASFDGQGFMMVDNSWVQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFDVYWDV---- 57 ASFDG F M D S+VQTK IDV S+TDISPYLS I EDS+ NGNR+++FDVYWDV Sbjct: 2 ASFDGPKFKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVYWDVGFPE 61 Query: 58 -KSVSTKSEWRLCSVKFSTKNFCLFLRLPNPFCDNLKDLYRFFASKFVTFVGVQIQEDLA 116 ++ + S W L SVK ST+N CLFLRLP PF DNLKDLYRFFASKFVTFVGVQI+EDL Sbjct: 62 TETKTKTSGWSLSSVKLSTRNLCLFLRLPKPFHDNLKDLYRFFASKFVTFVGVQIEEDLD 121 Query: 117 LLKENHGIVIRSSLEIGKLAAKARGTPIVEFLGTRELAHKILWYDMSRLDSIQSKWDEAS 176 LL+ENHG+VIR+++ +GKLAA+ARGT ++EFLGTRELAH++LW D+ +LDSI++KW++A Sbjct: 122 LLRENHGLVIRNAINVGKLAAEARGTLVLEFLGTRELAHRVLWSDLGQLDSIEAKWEKAG 181 Query: 177 SNDRLEAAAIEGWLIFNVYDQL 198 ++LEAAAIEGWLI NV+DQL Sbjct: 182 PEEQLEAAAIEGWLIVNVWDQL 203 >5HUS-A nrPDB Length = 306 Score = 30.4 bits (67), Expect = 0.48, Method: Compositional matrix adjust. Identities = 31/115 (26%), Positives = 48/115 (41%), Gaps = 15/115 (13%) Query: 83 RLPNPFCDNLKDLYRFFASKFVT--------FVGVQIQEDLALLKEN--HGIVIRSSLEI 132 RLP FC+ L RF A K + +G IQ L + N H ++R+S+ + Sbjct: 136 RLPGSFCEALDATVRFHAGKSFSEDRERSLDTMGETIQHINTLFEHNGVHATLVRNSVIV 195 Query: 133 GKLAAKARGTPIVEFLGTRELAHKILWYDMSRLDSIQSKW-DEASSNDRLEAAAI 186 K + +V L K Y ++ ++ Q D+ SND E AA+ Sbjct: 196 QKDQISLKAIRLVLSCHNSNLDQK---YVVTHINEFQQPMKDDIPSNDH-EVAAV 246 >5B8F-A nrPDB Length = 164 Score = 29.3 bits (64), Expect = 1.3, Method: Compositional matrix adjust. Identities = 16/48 (33%), Positives = 24/48 (50%) Query: 94 DLYRFFASKFVTFVGVQIQEDLALLKENHGIVIRSSLEIGKLAAKARG 141 D++RF F+T GV+I L+ + G V+ +L L A A G Sbjct: 15 DVHRFGEGDFITLGGVRIPHKHGLVAHSDGDVLLHALSDALLGAAALG 62 >3QDK-A nrPDB Length = 572 Score = 27.7 bits (60), Expect = 3.8, Method: Compositional matrix adjust. Identities = 14/37 (37%), Positives = 19/37 (51%) Query: 17 VQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFDV 53 V A+ E + + P S +L WNGNRSI+ D Sbjct: 360 VNVHALLEEKASQLRPGESGLLALDWWNGNRSILVDT 396 >3MQG-A nrPDB Length = 192 Score = 27.3 bits (59), Expect = 4.3, Method: Compositional matrix adjust. Identities = 18/65 (27%), Positives = 32/65 (49%), Gaps = 3/65 (4%) Query: 6 GQGFMMVDNSWVQTK---AIDVESTTDISPYLSKILEDSVWNGNRSIVFDVYWDVKSVST 62 G+G + N +V + V+ ++S Y + LED V+ G + +VY ++ Sbjct: 37 GEGCSLGQNVFVGNRVRIGNRVKIQNNVSVYDNVFLEDDVFCGPSMVFTNVYNPRAAIER 96 Query: 63 KSEWR 67 KSE+R Sbjct: 97 KSEYR 101 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5V5F-A nrPDB 365 e-102 2Q3S-A nrPDB 352 5e-98 Sequences not found previously or not previously below threshold: 4AR2-A nrPDB 31 0.43 6B1T-M nrPDB 29 1.7 5B8F-A nrPDB 29 1.8 5TX1-N nrPDB 28 4.1 6REQ-B nrPDB 27 7.1 5O57-A nrPDB 26 7.1 5YFP-F nrPDB 26 9.8 CONVERGED! >5V5F-A nrPDB Length = 200 Score = 365 bits (938), Expect = e-102, Method: Composition-based stats. Identities = 200/200 (100%), Positives = 200/200 (100%) Query: 1 MASFDGQGFMMVDNSWVQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFDVYWDVKSV 60 MASFDGQGFMMVDNSWVQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFDVYWDVKSV Sbjct: 1 MASFDGQGFMMVDNSWVQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFDVYWDVKSV 60 Query: 61 STKSEWRLCSVKFSTKNFCLFLRLPNPFCDNLKDLYRFFASKFVTFVGVQIQEDLALLKE 120 STKSEWRLCSVKFSTKNFCLFLRLPNPFCDNLKDLYRFFASKFVTFVGVQIQEDLALLKE Sbjct: 61 STKSEWRLCSVKFSTKNFCLFLRLPNPFCDNLKDLYRFFASKFVTFVGVQIQEDLALLKE 120 Query: 121 NHGIVIRSSLEIGKLAAKARGTPIVEFLGTRELAHKILWYDMSRLDSIQSKWDEASSNDR 180 NHGIVIRSSLEIGKLAAKARGTPIVEFLGTRELAHKILWYDMSRLDSIQSKWDEASSNDR Sbjct: 121 NHGIVIRSSLEIGKLAAKARGTPIVEFLGTRELAHKILWYDMSRLDSIQSKWDEASSNDR 180 Query: 181 LEAAAIEGWLIFNVYDQLQQ 200 LEAAAIEGWLIFNVYDQLQQ Sbjct: 181 LEAAAIEGWLIFNVYDQLQQ 200 >2Q3S-A nrPDB Length = 206 Score = 352 bits (904), Expect = 5e-98, Method: Composition-based stats. Identities = 137/202 (67%), Positives = 168/202 (83%), Gaps = 5/202 (2%) Query: 2 ASFDGQGFMMVDNSWVQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFDVYWDV---- 57 ASFDG F M D S+VQTK IDV S+TDISPYLS I EDS+ NGNR+++FDVYWDV Sbjct: 2 ASFDGPKFKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVYWDVGFPE 61 Query: 58 -KSVSTKSEWRLCSVKFSTKNFCLFLRLPNPFCDNLKDLYRFFASKFVTFVGVQIQEDLA 116 ++ + S W L SVK ST+N CLFLRLP PF DNLKDLYRFFASKFVTFVGVQI+EDL Sbjct: 62 TETKTKTSGWSLSSVKLSTRNLCLFLRLPKPFHDNLKDLYRFFASKFVTFVGVQIEEDLD 121 Query: 117 LLKENHGIVIRSSLEIGKLAAKARGTPIVEFLGTRELAHKILWYDMSRLDSIQSKWDEAS 176 LL+ENHG+VIR+++ +GKLAA+ARGT ++EFLGTRELAH++LW D+ +LDSI++KW++A Sbjct: 122 LLRENHGLVIRNAINVGKLAAEARGTLVLEFLGTRELAHRVLWSDLGQLDSIEAKWEKAG 181 Query: 177 SNDRLEAAAIEGWLIFNVYDQL 198 ++LEAAAIEGWLI NV+DQL Sbjct: 182 PEEQLEAAAIEGWLIVNVWDQL 203 >4AR2-A nrPDB Length = 509 Score = 30.7 bits (68), Expect = 0.43, Method: Composition-based stats. Identities = 21/108 (19%), Positives = 39/108 (36%), Gaps = 20/108 (18%) Query: 9 FMMVDNSWVQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFDVY-----WDVKSVSTK 63 F++ + ++ T ID+ + I YL ++ V + + FD WD ++ Sbjct: 177 FILPEGNFSATMTIDLMNNAIIDNYLEIGRQNGVLESDIGVKFDTRNFRLGWDPETKLIM 236 Query: 64 SEWRL-------------CSVKFSTKNFC--LFLRLPNPFCDNLKDLY 96 C V F+ L +R +PF + K +Y Sbjct: 237 PGVYTYEAFHPDIVLLPGCGVDFTESRLSNLLGIRKRHPFQEGFKIMY 284 >6B1T-M nrPDB Length = 571 Score = 28.8 bits (63), Expect = 1.7, Method: Composition-based stats. Identities = 9/44 (20%), Positives = 21/44 (47%) Query: 9 FMMVDNSWVQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFD 52 F + + ++ +T ID+ + + YL ++ V + + FD Sbjct: 168 FTLPEGNYSETMTIDLMNNAIVEHYLKVGRQNGVLESDIGVKFD 211 >5B8F-A nrPDB Length = 164 Score = 28.8 bits (63), Expect = 1.8, Method: Composition-based stats. Identities = 16/48 (33%), Positives = 24/48 (50%) Query: 94 DLYRFFASKFVTFVGVQIQEDLALLKENHGIVIRSSLEIGKLAAKARG 141 D++RF F+T GV+I L+ + G V+ +L L A A G Sbjct: 15 DVHRFGEGDFITLGGVRIPHKHGLVAHSDGDVLLHALSDALLGAAALG 62 >5TX1-N nrPDB Length = 497 Score = 27.6 bits (60), Expect = 4.1, Method: Composition-based stats. Identities = 19/108 (17%), Positives = 38/108 (35%), Gaps = 20/108 (18%) Query: 9 FMMVDNSWVQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFD-----VYWDVKSVSTK 63 F + + ++ +T ID+ + + YL ++ V + + FD + WD + Sbjct: 139 FTLPEGNFSETMTIDLMNNAILENYLQVGRQNGVLESDIGVKFDSRNFKLGWDPVTKLVM 198 Query: 64 SEWRL-------------CSVKFSTKNFC--LFLRLPNPFCDNLKDLY 96 C V F+ L +R PF + + +Y Sbjct: 199 PGVYTYEAFHPDVVLLPGCGVDFTESRLSNLLGIRKKQPFQEGFRIMY 246 >6REQ-B nrPDB Length = 637 Score = 26.9 bits (58), Expect = 7.1, Method: Composition-based stats. Identities = 18/58 (31%), Positives = 30/58 (51%), Gaps = 3/58 (5%) Query: 112 QEDLAL-LKENHGIVIRSSLEIGKLAAKARGTPIVEFLGTRELAHKILWYDMSRLDSI 168 ++D L + N GIV+ + IG++ A G+ VE L TR LA W + ++ + Sbjct: 370 EDDFPLRIARNTGIVLAEEVNIGRVNDPAGGSYYVESL-TRSLADAA-WKEFQEVEKL 425 >5O57-A nrPDB Length = 95 Score = 26.5 bits (57), Expect = 7.1, Method: Composition-based stats. Identities = 12/37 (32%), Positives = 16/37 (43%) Query: 61 STKSEWRLCSVKFSTKNFCLFLRLPNPFCDNLKDLYR 97 + K L +T+ FCL R PFC + L R Sbjct: 18 ARKGSQCLSDTDCNTRKFCLQPRDEKPFCATCRGLRR 54 >5YFP-F nrPDB Length = 910 Score = 26.1 bits (56), Expect = 9.8, Method: Composition-based stats. Identities = 22/104 (21%), Positives = 40/104 (38%), Gaps = 23/104 (22%) Query: 9 FMMVDNSWVQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFDVYWDVKSVSTKSEWRL 68 F++VDN++ T +SPYLS + D K + + +L Sbjct: 415 FILVDNNYNSTNEFWDGLMDRLSPYLS-----------------YFIDEKLKTEEDMIKL 457 Query: 69 CSVKFSTKNFCLFLRLPNPFCDNLKDLYRFFASKFVTFVGVQIQ 112 C+++ + F N++ LY+ S F F V ++ Sbjct: 458 KDF------LCIYVAILENFKLNIEPLYKILVSIFEKFCSVSLR 495 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.319 0.143 0.426 Lambda K H 0.267 0.0437 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,371,798 Number of Sequences: 36641 Number of extensions: 336417 Number of successful extensions: 706 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 16 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 688 Number of HSP's gapped (non-prelim): 27 length of query: 200 length of database: 10,336,785 effective HSP length: 92 effective length of query: 108 effective length of database: 6,965,813 effective search space: 752307804 effective search space used: 752307804 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 57 (26.5 bits)