BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9RFC8 (158 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4UC1-A nrPDB 308 5e-85 2MGY-A nrPDB 73 6e-14 4RYM-A nrPDB 34 0.022 6MPX-A nrPDB 29 1.0 4Z3P-A nrPDB 27 2.6 5NAX-A nrPDB 27 3.6 1ZRL-A nrPDB 27 5.4 5NAZ-A nrPDB 26 8.1 6OKK-C nrPDB 26 8.3 3D7W-B nrPDB 25 9.9 >4UC1-A nrPDB Length = 157 Score = 308 bits (790), Expect = 5e-85, Method: Compositional matrix adjust. Identities = 156/157 (99%), Positives = 156/157 (99%) Query: 1 MNMDWALFLTFLAACGAPATTGALLKPDEWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLA 60 MNMDWALFLTFLAACGAPATTGALLKPDEWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLA Sbjct: 1 MNMDWALFLTFLAACGAPATTGALLKPDEWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLA 60 Query: 61 AMRVAQLEGSGQALAFYAAQLAFNTLWTPVFFGMKRMATALAVVMVMWLFVAATMWAFFQ 120 AMRVAQLEGSGQALAFYAAQLAFNTLWTPVFFGMKRMATALAVVMVMWLFVAATMWAFFQ Sbjct: 61 AMRVAQLEGSGQALAFYAAQLAFNTLWTPVFFGMKRMATALAVVMVMWLFVAATMWAFFQ 120 Query: 121 LDTWAGVLFVPYLIWATAATGLNFEAMRLNWNRPEAR 157 LDTWAGVLFVPYLIWATA TGLNFEAMRLNWNRPEAR Sbjct: 121 LDTWAGVLFVPYLIWATATTGLNFEAMRLNWNRPEAR 157 >2MGY-A nrPDB Length = 169 Score = 72.8 bits (177), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 48/130 (36%), Positives = 64/130 (49%), Gaps = 5/130 (3%) Query: 29 EWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLAAMRV-AQLEGSGQ----ALAFYAAQLAF 83 WY L KP W+PPRW W +LY M + V +L G + L Y QLA Sbjct: 32 RWYAGLQKPSWHPPRWTLAPIWGTLYSAMGYGSYIVWKELGGFTEDAMVPLGLYTGQLAL 91 Query: 84 NTLWTPVFFGMKRMATALAVVMVMWLFVAATMWAFFQLDTWAGVLFVPYLIWATAATGLN 143 N W P+FFG ++M ALA ++++ AT A+ ++ A L PYL W AT LN Sbjct: 92 NWAWPPIFFGARQMGWALADLLLVSGVATATTLAWHRVSPPAARLLYPYLAWLAFATVLN 151 Query: 144 FEAMRLNWNR 153 + R N R Sbjct: 152 YYVWRDNSGR 161 >4RYM-A nrPDB Length = 181 Score = 34.3 bits (77), Expect = 0.022, Method: Compositional matrix adjust. Identities = 37/155 (23%), Positives = 61/155 (39%), Gaps = 7/155 (4%) Query: 1 MNMDWALFLTFLAACGAPATTGALLKPDE-WYDNLNKPWWNPPRWVFPLAWTSLYFLMSL 59 M M + + F G + L D WYD L KP W PP + W L+ L++L Sbjct: 29 MFMKKSSIIVFFLTYGLFYVSSVLFPIDRTWYDALEKPSWTPPGMTIGMIWAVLFGLIAL 88 Query: 60 AAMRVAQLEGSGQAL--AFYAAQLAFNTLWTPVFFGMKRMATALAVVMVMWLFVAATMWA 117 + + G + FN ++ F K + LA V + + + + Sbjct: 89 SVAIIYNNYGFKPKTFWFLFLLNYIFNQAFSYFQFSQKNL--FLATVDCLLVAITTLLLI 146 Query: 118 FF--QLDTWAGVLFVPYLIWATAATGLNFEAMRLN 150 F L + L +PY +W+ AT L++ +N Sbjct: 147 MFSSNLSKVSAWLLIPYFLWSAFATYLSWTIYSIN 181 >6MPX-A nrPDB Length = 693 Score = 28.9 bits (63), Expect = 1.0, Method: Composition-based stats. Identities = 23/68 (33%), Positives = 30/68 (44%), Gaps = 12/68 (17%) Query: 8 FLTFLAACGAPATTGALLKPDEWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLAAMRVAQL 67 F++ A C APA A+ ++ PP P W+SL+ S A Sbjct: 121 FISRCAVCEAPAMVMAVH---------SQTIQIPP---CPSGWSSLWIGYSFVMHTSAGA 168 Query: 68 EGSGQALA 75 EGSGQALA Sbjct: 169 EGSGQALA 176 Score = 28.9 bits (63), Expect = 1.0, Method: Composition-based stats. Identities = 23/68 (33%), Positives = 30/68 (44%), Gaps = 12/68 (17%) Query: 8 FLTFLAACGAPATTGALLKPDEWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLAAMRVAQL 67 F++ A C APA A+ ++ PP P W+SL+ S A Sbjct: 568 FISRCAVCEAPAMVMAVH---------SQTIQIPP---CPSGWSSLWIGYSFVMHTSAGA 615 Query: 68 EGSGQALA 75 EGSGQALA Sbjct: 616 EGSGQALA 623 >4Z3P-A nrPDB Length = 499 Score = 27.3 bits (59), Expect = 2.6, Method: Compositional matrix adjust. Identities = 19/65 (29%), Positives = 32/65 (49%), Gaps = 6/65 (9%) Query: 4 DWALFLTFLAACGAPATTGALLKPDEWYDNLNKPWWNP----PRWVFPLAWTSLYFLMSL 59 D+A+ T + A P +LL E+Y + KPW++ R VF ++ +Y L + Sbjct: 370 DYAVLQTRIMALALPFVAISLL-ASEYYQAIGKPWYSVLLTLMRHVF-ISVPVVYLLAIV 427 Query: 60 AAMRV 64 MR+ Sbjct: 428 LEMRI 432 >5NAX-A nrPDB Length = 229 Score = 26.9 bits (58), Expect = 3.6, Method: Compositional matrix adjust. Identities = 23/68 (33%), Positives = 30/68 (44%), Gaps = 12/68 (17%) Query: 8 FLTFLAACGAPATTGALLKPDEWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLAAMRVAQL 67 F++ A C APA A+ ++ PP P W+SL+ S A Sbjct: 104 FISRCAVCEAPAMVMAVH---------SQTIQIPP---CPSGWSSLWIGYSFVMHTSAGA 151 Query: 68 EGSGQALA 75 EGSGQALA Sbjct: 152 EGSGQALA 159 >1ZRL-A nrPDB Length = 602 Score = 26.6 bits (57), Expect = 5.4, Method: Composition-based stats. Identities = 9/19 (47%), Positives = 11/19 (57%) Query: 28 DEWYDNLNKPWWNPPRWVF 46 DEW+ + K WN WVF Sbjct: 447 DEWWKVIKKDVWNVISWVF 465 >5NAZ-A nrPDB Length = 229 Score = 25.8 bits (55), Expect = 8.1, Method: Compositional matrix adjust. Identities = 22/68 (32%), Positives = 27/68 (39%), Gaps = 12/68 (17%) Query: 8 FLTFLAACGAPATTGALLKPDEWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLAAMRVAQL 67 F++ A C APA A+ + P P W SL+ S A Sbjct: 104 FISRCAVCEAPAVVIAVHS-----QTIQIPHC-------PQGWDSLWIGYSFMMHTSAGA 151 Query: 68 EGSGQALA 75 EGSGQALA Sbjct: 152 EGSGQALA 159 >6OKK-C nrPDB Length = 263 Score = 25.8 bits (55), Expect = 8.3, Method: Compositional matrix adjust. Identities = 9/16 (56%), Positives = 11/16 (68%) Query: 26 KPDEWYDNLNKPWWNP 41 K +EW DN N+ W NP Sbjct: 242 KKEEWNDNTNEDWKNP 257 >3D7W-B nrPDB Length = 265 Score = 25.4 bits (54), Expect = 9.9, Method: Compositional matrix adjust. Identities = 19/48 (39%), Positives = 21/48 (43%), Gaps = 1/48 (2%) Query: 43 RWVFPLAWTSLYFLMSLAAMRVAQLEGSGQALAFYAAQLAFNTLWTPV 90 RWVF L LA M VAQ S Q + Y A N +W PV Sbjct: 216 RWVFTNEGAILNLKNGLA-MDVAQANPSLQRIIIYPATGNPNQMWLPV 262 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4UC1-A nrPDB 218 7e-58 2MGY-A nrPDB 161 1e-40 Sequences not found previously or not previously below threshold: 4RYM-A nrPDB 54 3e-08 4RQ9-A nrPDB 27 3.0 6BAF-A nrPDB 27 3.3 6BAP-A nrPDB 26 6.4 >4UC1-A nrPDB Length = 157 Score = 218 bits (556), Expect = 7e-58, Method: Composition-based stats. Identities = 156/157 (99%), Positives = 156/157 (99%) Query: 1 MNMDWALFLTFLAACGAPATTGALLKPDEWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLA 60 MNMDWALFLTFLAACGAPATTGALLKPDEWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLA Sbjct: 1 MNMDWALFLTFLAACGAPATTGALLKPDEWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLA 60 Query: 61 AMRVAQLEGSGQALAFYAAQLAFNTLWTPVFFGMKRMATALAVVMVMWLFVAATMWAFFQ 120 AMRVAQLEGSGQALAFYAAQLAFNTLWTPVFFGMKRMATALAVVMVMWLFVAATMWAFFQ Sbjct: 61 AMRVAQLEGSGQALAFYAAQLAFNTLWTPVFFGMKRMATALAVVMVMWLFVAATMWAFFQ 120 Query: 121 LDTWAGVLFVPYLIWATAATGLNFEAMRLNWNRPEAR 157 LDTWAGVLFVPYLIWATA TGLNFEAMRLNWNRPEAR Sbjct: 121 LDTWAGVLFVPYLIWATATTGLNFEAMRLNWNRPEAR 157 >2MGY-A nrPDB Length = 169 Score = 161 bits (408), Expect = 1e-40, Method: Composition-based stats. Identities = 48/130 (36%), Positives = 64/130 (49%), Gaps = 5/130 (3%) Query: 29 EWYDNLNKPWWNPPRWVFPLAWTSLYFLMSLAAMRV-AQLEGSGQ----ALAFYAAQLAF 83 WY L KP W+PPRW W +LY M + V +L G + L Y QLA Sbjct: 32 RWYAGLQKPSWHPPRWTLAPIWGTLYSAMGYGSYIVWKELGGFTEDAMVPLGLYTGQLAL 91 Query: 84 NTLWTPVFFGMKRMATALAVVMVMWLFVAATMWAFFQLDTWAGVLFVPYLIWATAATGLN 143 N W P+FFG ++M ALA ++++ AT A+ ++ A L PYL W AT LN Sbjct: 92 NWAWPPIFFGARQMGWALADLLLVSGVATATTLAWHRVSPPAARLLYPYLAWLAFATVLN 151 Query: 144 FEAMRLNWNR 153 + R N R Sbjct: 152 YYVWRDNSGR 161 >4RYM-A nrPDB Length = 181 Score = 53.9 bits (128), Expect = 3e-08, Method: Composition-based stats. Identities = 35/153 (22%), Positives = 61/153 (39%), Gaps = 3/153 (1%) Query: 1 MNMDWALFLTFLAACGAPATTGALLKPDE-WYDNLNKPWWNPPRWVFPLAWTSLYFLMSL 59 M M + + F G + L D WYD L KP W PP + W L+ L++L Sbjct: 29 MFMKKSSIIVFFLTYGLFYVSSVLFPIDRTWYDALEKPSWTPPGMTIGMIWAVLFGLIAL 88 Query: 60 AAMRVAQLEGSGQALAFYAAQL--AFNTLWTPVFFGMKRMATALAVVMVMWLFVAATMWA 117 + + G ++ L FN ++ F K + A +++ + + Sbjct: 89 SVAIIYNNYGFKPKTFWFLFLLNYIFNQAFSYFQFSQKNLFLATVDCLLVAITTLLLIMF 148 Query: 118 FFQLDTWAGVLFVPYLIWATAATGLNFEAMRLN 150 L + L +PY +W+ AT L++ +N Sbjct: 149 SSNLSKVSAWLLIPYFLWSAFATYLSWTIYSIN 181 >4RQ9-A nrPDB Length = 348 Score = 27.3 bits (59), Expect = 3.0, Method: Composition-based stats. Identities = 25/108 (23%), Positives = 42/108 (38%), Gaps = 13/108 (12%) Query: 31 YDNLNKPWWNPPRW---VFPLAWTSLYFLMSLAAMRVAQLEGSGQALAFYAAQLAFNTLW 87 Y L+ P + PR ++ L W + + RV L G G+ L L+F+ L Sbjct: 219 YLGLHFPASDIPRQARELYQLNWLRIIPTIDYQPARVRALPGHGEPL-----DLSFSVLR 273 Query: 88 --TPVFFG-MKRMATALAVVMVMWLFVAATMWAFFQLDTWAGVLFVPY 132 +P+ + M + M + L +W +G +VPY Sbjct: 274 SVSPIHLEYLHNMGVQAS--MSISLMKDGKLWGLISCHQVSGTRYVPY 319 >6BAF-A nrPDB Length = 303 Score = 27.0 bits (58), Expect = 3.3, Method: Composition-based stats. Identities = 25/108 (23%), Positives = 42/108 (38%), Gaps = 13/108 (12%) Query: 31 YDNLNKPWWNPPRW---VFPLAWTSLYFLMSLAAMRVAQLEGSGQALAFYAAQLAFNTLW 87 Y L+ P + PR ++ L W + + RV L G G+ L L+F+ L Sbjct: 184 YLGLHFPASDIPRQARELYQLNWLRIIPTIDYQPARVRALPGHGEPL-----DLSFSVLR 238 Query: 88 --TPVFFG-MKRMATALAVVMVMWLFVAATMWAFFQLDTWAGVLFVPY 132 +P+ + M + M + L +W +G +VPY Sbjct: 239 SVSPIHLEYLHNMGVQAS--MSISLMKDGKLWGLISCTQVSGTRYVPY 284 >6BAP-A nrPDB Length = 515 Score = 26.2 bits (56), Expect = 6.4, Method: Composition-based stats. Identities = 25/108 (23%), Positives = 42/108 (38%), Gaps = 13/108 (12%) Query: 31 YDNLNKPWWNPPRW---VFPLAWTSLYFLMSLAAMRVAQLEGSGQALAFYAAQLAFNTLW 87 Y L+ P + PR ++ L W + + RV L G G+ L L+F+ L Sbjct: 199 YLGLHFPASDIPRQARELYQLNWLRIIPTIDYQPARVRALPGHGEPL-----DLSFSVLR 253 Query: 88 --TPVFFG-MKRMATALAVVMVMWLFVAATMWAFFQLDTWAGVLFVPY 132 +P+ + M + M + L +W +G +VPY Sbjct: 254 SVSPIHLEYLHNMGVQAS--MSISLMKDGKLWGLISCHQVSGTRYVPY 299 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.311 0.133 0.458 Lambda K H 0.267 0.0414 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,919,213 Number of Sequences: 36641 Number of extensions: 254653 Number of successful extensions: 696 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 673 Number of HSP's gapped (non-prelim): 29 length of query: 158 length of database: 10,336,785 effective HSP length: 89 effective length of query: 69 effective length of database: 7,075,736 effective search space: 488225784 effective search space used: 488225784 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (20.9 bits) S2: 55 (25.8 bits)