BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9R257 (190 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2HVA-A nrPDB 392 e-110 3R8J-A nrPDB 89 1e-18 4WFK-A nrPDB 28 1.7 5J54-A nrPDB 27 5.1 2K06-A nrPDB 27 6.8 2IWM-A nrPDB 27 6.8 5WLC-SH nrPDB 27 7.4 2WHK-A nrPDB 26 8.3 6C6U-N nrPDB 26 8.8 >2HVA-A nrPDB Length = 192 Score = 392 bits (1008), Expect = e-110, Method: Compositional matrix adjust. Identities = 189/190 (99%), Positives = 190/190 (100%) Query: 1 MLGMIRNSLFGSVETWPWQVLSTGGKEDVSYEERACEGGKFATVEVTDKPVDEALREAMP 60 MLGMIRNSLFGSVETWPWQVLSTGGKEDVSYEERACEGGKFATVEVTDKPVDEALREAMP Sbjct: 3 MLGMIRNSLFGSVETWPWQVLSTGGKEDVSYEERACEGGKFATVEVTDKPVDEALREAMP 62 Query: 61 KIMKYVGGTNDKGVGMGMTVPVSFALFPNEDGSLQKKLKVWFRIPNQFQGSPPAPSDESV 120 KIMKYVGGTNDKGVGMGMTVPVSFA+FPNEDGSLQKKLKVWFRIPNQFQGSPPAPSDESV Sbjct: 63 KIMKYVGGTNDKGVGMGMTVPVSFAVFPNEDGSLQKKLKVWFRIPNQFQGSPPAPSDESV 122 Query: 121 KIEEREGITVYSTQFGGYAKEADYVAHATQLRTTLEGTPATYQGDVYYCAGYDPPMKPYG 180 KIEEREGITVYSTQFGGYAKEADYVAHATQLRTTLEGTPATYQGDVYYCAGYDPPMKPYG Sbjct: 123 KIEEREGITVYSTQFGGYAKEADYVAHATQLRTTLEGTPATYQGDVYYCAGYDPPMKPYG 182 Query: 181 RRNEVWLVKA 190 RRNEVWLVKA Sbjct: 183 RRNEVWLVKA 192 >3R8J-A nrPDB Length = 212 Score = 88.6 bits (218), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 53/177 (29%), Positives = 83/177 (46%) Query: 13 VETWPWQVLSTGGKEDVSYEERACEGGKFATVEVTDKPVDEALREAMPKIMKYVGGTNDK 72 VET W+ G + SYE R K+ + V D A++ K+ Y+ G N+K Sbjct: 23 VETPGWKAPEDAGPQPGSYEIRHYGPAKWVSTSVESMDWDSAIQTGFTKLNSYIQGKNEK 82 Query: 73 GVGMGMTVPVSFALFPNEDGSLQKKLKVWFRIPNQFQGSPPAPSDESVKIEEREGITVYS 132 + + MT PV+ + P + + + IP++ Q PP P + V IE+R +TV+ Sbjct: 83 EMKIKMTAPVTSYVEPGSGPFSESTITISLYIPSEQQFDPPRPLESDVFIEDRAEMTVFV 142 Query: 133 TQFGGYAKEADYVAHATQLRTTLEGTPATYQGDVYYCAGYDPPMKPYGRRNEVWLVK 189 F G++ L + L + VYY AGY+ P+K R NEVWL++ Sbjct: 143 RSFDGFSSAQKNQEQLLTLASILREDGKVFDEKVYYTAGYNSPVKLLNRNNEVWLIQ 199 >4WFK-A nrPDB Length = 273 Score = 28.5 bits (62), Expect = 1.7, Method: Compositional matrix adjust. Identities = 21/68 (30%), Positives = 31/68 (45%), Gaps = 1/68 (1%) Query: 4 MIRNSLFGSVETWPWQVLSTGGKEDVSYEERACEGGKFATVEVTDKPVDEALREAMPKI- 62 ++R SL S+ PW + T G+ V E A V KP E+L ++PK Sbjct: 153 VMRPSLKASIPLTPWNLDKTWGQVQVPTFIIGAELDTIAPVRTHAKPFYESLPSSLPKAY 212 Query: 63 MKYVGGTN 70 M+ G T+ Sbjct: 213 MELDGATH 220 >5J54-A nrPDB Length = 494 Score = 26.9 bits (58), Expect = 5.1, Method: Compositional matrix adjust. Identities = 15/49 (30%), Positives = 20/49 (40%), Gaps = 1/49 (2%) Query: 75 GMGMTVPVSFALFPNEDGSLQKKLKVWFRIPNQFQGSPPAPSDESVKIE 123 G T+PV + P DG Q+ ++ WF N F E KI Sbjct: 249 GFDTTMPVHLGIIPRRDGVRQEDIR-WFTRDNCFASHVLNAWQEGTKIH 296 >2K06-A nrPDB Length = 123 Score = 26.6 bits (57), Expect = 6.8, Method: Compositional matrix adjust. Identities = 16/64 (25%), Positives = 31/64 (48%), Gaps = 2/64 (3%) Query: 9 LFGSVETWPWQVLSTGGKEDVSYEERACEGGKFATVEVTDKPVDEALREAMPKIMKYVGG 68 LFG V +V+ G + E + G + V++ L ++P++M ++GG Sbjct: 38 LFGEVMVPTEEVVEIRGGQRRKSERKFFPG--YVLVQMVMNDASWHLVRSVPRVMGFIGG 95 Query: 69 TNDK 72 T+D+ Sbjct: 96 TSDR 99 >2IWM-A nrPDB Length = 338 Score = 26.6 bits (57), Expect = 6.8, Method: Compositional matrix adjust. Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 1/45 (2%) Query: 116 SDESVKIE-EREGITVYSTQFGGYAKEADYVAHATQLRTTLEGTP 159 S ES+ IE ++ GIT++ G Y H T LR + TP Sbjct: 153 SGESIVIEPDKTGITIHRKTIGVMTNSPGYEWHQTNLRAYIGVTP 197 >5WLC-SH nrPDB Length = 367 Score = 26.6 bits (57), Expect = 7.4, Method: Compositional matrix adjust. Identities = 15/31 (48%), Positives = 17/31 (54%) Query: 40 KFATVEVTDKPVDEALREAMPKIMKYVGGTN 70 K EV KPVDEA E M +K +G TN Sbjct: 331 KIFNTEVFLKPVDEADNEDMIATIKGIGFTN 361 >2WHK-A nrPDB Length = 336 Score = 26.2 bits (56), Expect = 8.3, Method: Compositional matrix adjust. Identities = 13/36 (36%), Positives = 20/36 (55%) Query: 130 VYSTQFGGYAKEADYVAHATQLRTTLEGTPATYQGD 165 V S FGGY+ + +A A ++R+ +PA Y D Sbjct: 32 VLSGAFGGYSHDTFSMAEADRIRSATGQSPAIYGCD 67 >6C6U-N nrPDB Length = 181 Score = 26.2 bits (56), Expect = 8.8, Method: Compositional matrix adjust. Identities = 16/64 (25%), Positives = 31/64 (48%), Gaps = 2/64 (3%) Query: 9 LFGSVETWPWQVLSTGGKEDVSYEERACEGGKFATVEVTDKPVDEALREAMPKIMKYVGG 68 LFG V +V+ G + E + G + V++ L ++P++M ++GG Sbjct: 38 LFGEVMVPTEEVVEIRGGQRRKSERKFFPG--YVLVQMVMNDASWHLVRSVPRVMGFIGG 95 Query: 69 TNDK 72 T+D+ Sbjct: 96 TSDR 99 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2HVA-A nrPDB 347 1e-96 3R8J-A nrPDB 287 2e-78 Sequences not found previously or not previously below threshold: 1IU8-A nrPDB 30 0.63 3RO0-A nrPDB 30 0.64 6F35-A nrPDB 30 0.75 5I5I-A nrPDB 29 1.1 5E53-A nrPDB 28 2.2 CONVERGED! >2HVA-A nrPDB Length = 192 Score = 347 bits (891), Expect = 1e-96, Method: Composition-based stats. Identities = 189/190 (99%), Positives = 190/190 (100%) Query: 1 MLGMIRNSLFGSVETWPWQVLSTGGKEDVSYEERACEGGKFATVEVTDKPVDEALREAMP 60 MLGMIRNSLFGSVETWPWQVLSTGGKEDVSYEERACEGGKFATVEVTDKPVDEALREAMP Sbjct: 3 MLGMIRNSLFGSVETWPWQVLSTGGKEDVSYEERACEGGKFATVEVTDKPVDEALREAMP 62 Query: 61 KIMKYVGGTNDKGVGMGMTVPVSFALFPNEDGSLQKKLKVWFRIPNQFQGSPPAPSDESV 120 KIMKYVGGTNDKGVGMGMTVPVSFA+FPNEDGSLQKKLKVWFRIPNQFQGSPPAPSDESV Sbjct: 63 KIMKYVGGTNDKGVGMGMTVPVSFAVFPNEDGSLQKKLKVWFRIPNQFQGSPPAPSDESV 122 Query: 121 KIEEREGITVYSTQFGGYAKEADYVAHATQLRTTLEGTPATYQGDVYYCAGYDPPMKPYG 180 KIEEREGITVYSTQFGGYAKEADYVAHATQLRTTLEGTPATYQGDVYYCAGYDPPMKPYG Sbjct: 123 KIEEREGITVYSTQFGGYAKEADYVAHATQLRTTLEGTPATYQGDVYYCAGYDPPMKPYG 182 Query: 181 RRNEVWLVKA 190 RRNEVWLVKA Sbjct: 183 RRNEVWLVKA 192 >3R8J-A nrPDB Length = 212 Score = 287 bits (734), Expect = 2e-78, Method: Composition-based stats. Identities = 53/177 (29%), Positives = 83/177 (46%) Query: 13 VETWPWQVLSTGGKEDVSYEERACEGGKFATVEVTDKPVDEALREAMPKIMKYVGGTNDK 72 VET W+ G + SYE R K+ + V D A++ K+ Y+ G N+K Sbjct: 23 VETPGWKAPEDAGPQPGSYEIRHYGPAKWVSTSVESMDWDSAIQTGFTKLNSYIQGKNEK 82 Query: 73 GVGMGMTVPVSFALFPNEDGSLQKKLKVWFRIPNQFQGSPPAPSDESVKIEEREGITVYS 132 + + MT PV+ + P + + + IP++ Q PP P + V IE+R +TV+ Sbjct: 83 EMKIKMTAPVTSYVEPGSGPFSESTITISLYIPSEQQFDPPRPLESDVFIEDRAEMTVFV 142 Query: 133 TQFGGYAKEADYVAHATQLRTTLEGTPATYQGDVYYCAGYDPPMKPYGRRNEVWLVK 189 F G++ L + L + VYY AGY+ P+K R NEVWL++ Sbjct: 143 RSFDGFSSAQKNQEQLLTLASILREDGKVFDEKVYYTAGYNSPVKLLNRNNEVWLIQ 199 >1IU8-A nrPDB Length = 206 Score = 30.1 bits (66), Expect = 0.63, Method: Composition-based stats. Identities = 18/107 (16%), Positives = 37/107 (34%), Gaps = 9/107 (8%) Query: 19 QVLSTGGKEDVSYEERACEGGKFATVEVTDKPVDEALREAMPKIMKYVGGTNDKGVGMGM 78 ++ G++ + T + V+E + +P ++ Y GT M + Sbjct: 86 RIPDNDGEQPKDEPIVEGGPAAYFATIPTREIVEEMKKNGIPAVLSYTAGTYLCNFAMYL 145 Query: 79 TVPVSFAL-FPNEDGSLQKKLKVWFRIPNQ---FQGSPPAPSDESVK 121 T+ S +P G + P+Q + +P D +K Sbjct: 146 TLHTSATKGYPKIAGFIHVP-----YTPDQVLEKKNTPSMSLDLEIK 187 >3RO0-A nrPDB Length = 223 Score = 30.1 bits (66), Expect = 0.64, Method: Composition-based stats. Identities = 10/51 (19%), Positives = 20/51 (39%) Query: 19 QVLSTGGKEDVSYEERACEGGKFATVEVTDKPVDEALREAMPKIMKYVGGT 69 ++ G + V + + T + V+E +E +P + Y GT Sbjct: 91 RIPDNEGNQPVGEDISQGGPAAYWTGLPIKRIVEEIKKEGIPAAVSYTAGT 141 >6F35-A nrPDB Length = 410 Score = 29.7 bits (65), Expect = 0.75, Method: Composition-based stats. Identities = 16/54 (29%), Positives = 24/54 (44%), Gaps = 2/54 (3%) Query: 137 GYAKEADYVAHATQLRT--TLEGTPATYQGDVYYCAGYDPPMKPYGRRNEVWLV 188 G+ A+ + A RT L +P+ G Y A Y P ++ R VWL+ Sbjct: 159 GFRLTAEKLEAAITPRTRWVLLNSPSNPSGAAYSAADYRPLLEVLLRHPHVWLL 212 >5I5I-A nrPDB Length = 636 Score = 29.3 bits (64), Expect = 1.1, Method: Composition-based stats. Identities = 16/81 (19%), Positives = 31/81 (38%), Gaps = 3/81 (3%) Query: 96 KKLKVWFRIPNQFQGSPPAPSDESVKIEEREGITVYSTQFGGYAKEADYVAHATQLRTTL 155 +K+ + + P +D +E+ ++ Y + F E VA + L Sbjct: 179 QKVPYTKYVICNGEFEIPMNNDGKASLED---VSTYRSLFNVIDAEKMEVAFQVMVDGNL 235 Query: 156 EGTPATYQGDVYYCAGYDPPM 176 + T A Y G ++ Y+ M Sbjct: 236 DNTDADYDGKYFFSTCYNSEM 256 >5E53-A nrPDB Length = 301 Score = 28.2 bits (61), Expect = 2.2, Method: Composition-based stats. Identities = 35/195 (17%), Positives = 49/195 (25%), Gaps = 38/195 (19%) Query: 16 WPWQVLSTGGKEDVSYEE---------RACEGGKFATVEVTDKPVDEA----LREAMPKI 62 P S G S E R G V KP E + P+I Sbjct: 104 PPNVAPSDVGGGGGSNRELTITWMPLSREYHYGNNFGYIVAFKPFGEKEWRRVTVTNPEI 163 Query: 63 MKYVGGTNDKGVGMGMTVPVSFALFPNEDGSLQKKLKVWFRIPNQFQGSPPAPSDESVKI 122 +YV V V + + I + P+D SVK+ Sbjct: 164 GRYVHKDESMPPSTQYQVKVKAFNSKGDGPFSLTAV-----IYSAQDAPTEVPTDVSVKV 218 Query: 123 EEREGITV----------------YSTQFGGYAKEAD----YVAHATQLRTTLEGTPATY 162 I+V Y A ++T+L T Sbjct: 219 LSSSEISVSWHHVTEKSVEGYQIRYWAAHDKEAAAQRVQVSNQEYSTKLENLKPNTRYHI 278 Query: 163 QGDVYYCAGYDPPMK 177 + AGY PP + Sbjct: 279 DVSAFNSAGYGPPSR 293 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.301 0.128 0.351 Lambda K H 0.267 0.0386 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,209,362 Number of Sequences: 36641 Number of extensions: 274326 Number of successful extensions: 465 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 454 Number of HSP's gapped (non-prelim): 21 length of query: 190 length of database: 10,336,785 effective HSP length: 92 effective length of query: 98 effective length of database: 6,965,813 effective search space: 682649674 effective search space used: 682649674 T: 11 A: 40 X1: 16 ( 6.9 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.2 bits) S2: 56 (26.3 bits)