BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9QXC1 (388 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6HT9-B nrPDB 759 0.0 2CH9-A nrPDB 39 0.003 1ZUN-B nrPDB 32 0.68 1CEW-I nrPDB 31 0.80 1BRY-Y nrPDB 31 0.95 4IT7-A nrPDB 30 2.0 1YPX-A nrPDB 29 3.7 >6HT9-B nrPDB Length = 378 Score = 759 bits (1959), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/370 (100%), Positives = 370/370 (100%) Query: 19 RSPPAPPLPQRPLSPLHPLGCNDSEVLAVAGFALQNINRDQKDGYMLSLNRVHDVREHYQ 78 RSPPAPPLPQRPLSPLHPLGCNDSEVLAVAGFALQNINRDQKDGYMLSLNRVHDVREHYQ Sbjct: 1 RSPPAPPLPQRPLSPLHPLGCNDSEVLAVAGFALQNINRDQKDGYMLSLNRVHDVREHYQ 60 Query: 79 EDMGSLFYLTLDVLETDCHVLSRKAQKDCKPRMFYESVYGQCKAMFHINKPRRVLYLPAY 138 EDMGSLFYLTLDVLETDCHVLSRKAQKDCKPRMFYESVYGQCKAMFHINKPRRVLYLPAY Sbjct: 61 EDMGSLFYLTLDVLETDCHVLSRKAQKDCKPRMFYESVYGQCKAMFHINKPRRVLYLPAY 120 Query: 139 NCTLRPVSKRKTHTTCPDCPSPIDLSNPSALEAATESLAKFNSKSPSKKYELVKVTKAMN 198 NCTLRPVSKRKTHTTCPDCPSPIDLSNPSALEAATESLAKFNSKSPSKKYELVKVTKAMN Sbjct: 121 NCTLRPVSKRKTHTTCPDCPSPIDLSNPSALEAATESLAKFNSKSPSKKYELVKVTKAMN 180 Query: 199 QWVSGPAYYVEYLIKEAPCTKSQASCSLQHSDSEPVGICQGSTVQSSLRHVPLIQPVEKS 258 QWVSGPAYYVEYLIKEAPCTKSQASCSLQHSDSEPVGICQGSTVQSSLRHVPLIQPVEKS Sbjct: 181 QWVSGPAYYVEYLIKEAPCTKSQASCSLQHSDSEPVGICQGSTVQSSLRHVPLIQPVEKS 240 Query: 259 VTVTCEFFESQAQVPGDENPAVTQGPQKLPQKNTAPTSSPSVTAPRGSIQHLPELDDEKP 318 VTVTCEFFESQAQVPGDENPAVTQGPQKLPQKNTAPTSSPSVTAPRGSIQHLPELDDEKP Sbjct: 241 VTVTCEFFESQAQVPGDENPAVTQGPQKLPQKNTAPTSSPSVTAPRGSIQHLPELDDEKP 300 Query: 319 EESKGGSPEEAFPVQLDLTTNPQGDTLDVSFLYLEPGDKKLVVLPFPGKEQRSAECPGPE 378 EESKGGSPEEAFPVQLDLTTNPQGDTLDVSFLYLEPGDKKLVVLPFPGKEQRSAECPGPE Sbjct: 301 EESKGGSPEEAFPVQLDLTTNPQGDTLDVSFLYLEPGDKKLVVLPFPGKEQRSAECPGPE 360 Query: 379 KENNPLVLPP 388 KENNPLVLPP Sbjct: 361 KENNPLVLPP 370 >2CH9-A nrPDB Length = 131 Score = 39.3 bits (90), Expect = 0.003, Method: Compositional matrix adjust. Identities = 30/107 (28%), Positives = 50/107 (46%), Gaps = 9/107 (8%) Query: 155 PDCPSPIDLSNPSALEAATESLAKFNS-KSPSKKYELVKVTKAMNQWVSGPAYYVEYLIK 213 P P I ++P L+AA S+ KFN+ + ++ ++T+A+ Q V G Y +E I Sbjct: 17 PGFPKTIKTNDPGVLQAARYSVEKFNNCTNDMFLFKESRITRALVQIVKGLKYMLEVEIG 76 Query: 214 EAPCTKSQA----SCSLQHSDSEPVGICQGSTVQSSLRHVPLIQPVE 256 C K+Q C Q + + + Q + S + VP +Q E Sbjct: 77 RTTCKKNQHLRLDDCDFQTNHT----LKQTLSCYSEVWVVPWLQHFE 119 >1ZUN-B nrPDB Length = 434 Score = 31.6 bits (70), Expect = 0.68, Method: Compositional matrix adjust. Identities = 16/55 (29%), Positives = 24/55 (43%) Query: 315 DEKPEESKGGSPEEAFPVQLDLTTNPQGDTLDVSFLYLEPGDKKLVVLPFPGKEQ 369 D K + G + A V QG T+DV++ Y +K ++ PG EQ Sbjct: 62 DSKKSGTTGDDVDLALLVDGLQAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQ 116 >1CEW-I nrPDB Length = 108 Score = 31.2 bits (69), Expect = 0.80, Method: Compositional matrix adjust. Identities = 20/64 (31%), Positives = 31/64 (48%), Gaps = 1/64 (1%) Query: 158 PSPIDLSNPSALEAATESLAKFNSKSPSK-KYELVKVTKAMNQWVSGPAYYVEYLIKEAP 216 P P+D ++ A ++A++N S K +V+V A Q VSG Y ++ I Sbjct: 3 PVPVDENDEGLQRALQFAMAEYNRASNDKYSSRVVRVISAKRQLVSGIKYILQVEIGRTT 62 Query: 217 CTKS 220 C KS Sbjct: 63 CPKS 66 >1BRY-Y nrPDB Length = 248 Score = 30.8 bits (68), Expect = 0.95, Method: Compositional matrix adjust. Identities = 23/76 (30%), Positives = 31/76 (40%), Gaps = 13/76 (17%) Query: 326 PEEAFPVQLDLTTNPQGDTL--DVSFLYLEPGD-----------KKLVVLPFPGKEQRSA 372 +E V +D+T L DVS+ + E KK V LP+ G +R Sbjct: 57 ADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVFKDAKKKVTLPYSGNYERLQ 116 Query: 373 ECPGPEKENNPLVLPP 388 G +EN PL LP Sbjct: 117 TAAGKIRENIPLGLPA 132 >4IT7-A nrPDB Length = 113 Score = 30.0 bits (66), Expect = 2.0, Method: Composition-based stats. Identities = 20/66 (30%), Positives = 33/66 (50%), Gaps = 1/66 (1%) Query: 159 SPIDLSNPSALEAATESLAKFNSKSPSK-KYELVKVTKAMNQWVSGPAYYVEYLIKEAPC 217 S D+++P A ++L + N+ S + +VKV A Q V+G +E LI C Sbjct: 10 STKDVNDPKIQALAGKALQRINAASNDLFQQTIVKVISAKTQVVAGTNTVLELLIAPTSC 69 Query: 218 TKSQAS 223 K++ S Sbjct: 70 RKNETS 75 >1YPX-A nrPDB Length = 375 Score = 28.9 bits (63), Expect = 3.7, Method: Compositional matrix adjust. Identities = 24/84 (28%), Positives = 36/84 (42%), Gaps = 12/84 (14%) Query: 232 EPVGICQGSTVQSSLRHVPLIQPVEKSVTVTCEFFESQAQVPGDENPAVTQGPQKLPQKN 291 + G Q S VQ+ V + P++ + E F + GD + A K Sbjct: 86 DAAGGIQFSKVQTKSHSVKITGPIDFTTHPFIEDFIFLKEAVGDNHVA----------KQ 135 Query: 292 TAPTSSPSVTAPRGSIQHLPELDD 315 T P SP++ RG I++ P LDD Sbjct: 136 TIP--SPAMLHYRGDIEYQPYLDD 157 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6HT9-B nrPDB 753 0.0 Sequences not found previously or not previously below threshold: 2CH9-A nrPDB 40 0.002 1CEW-I nrPDB 34 0.088 4Z6Y-B nrPDB 32 0.42 4IT7-A nrPDB 32 0.68 2IG2-H nrPDB 31 1.1 1ZUN-B nrPDB 30 1.3 1HZH-H nrPDB 30 1.5 1BRY-Y nrPDB 30 2.2 1YPX-A nrPDB 29 3.9 1ROA-A nrPDB 28 6.3 5IJO-H nrPDB 28 9.9 CONVERGED! >6HT9-B nrPDB Length = 378 Score = 753 bits (1943), Expect = 0.0, Method: Composition-based stats. Identities = 370/370 (100%), Positives = 370/370 (100%) Query: 19 RSPPAPPLPQRPLSPLHPLGCNDSEVLAVAGFALQNINRDQKDGYMLSLNRVHDVREHYQ 78 RSPPAPPLPQRPLSPLHPLGCNDSEVLAVAGFALQNINRDQKDGYMLSLNRVHDVREHYQ Sbjct: 1 RSPPAPPLPQRPLSPLHPLGCNDSEVLAVAGFALQNINRDQKDGYMLSLNRVHDVREHYQ 60 Query: 79 EDMGSLFYLTLDVLETDCHVLSRKAQKDCKPRMFYESVYGQCKAMFHINKPRRVLYLPAY 138 EDMGSLFYLTLDVLETDCHVLSRKAQKDCKPRMFYESVYGQCKAMFHINKPRRVLYLPAY Sbjct: 61 EDMGSLFYLTLDVLETDCHVLSRKAQKDCKPRMFYESVYGQCKAMFHINKPRRVLYLPAY 120 Query: 139 NCTLRPVSKRKTHTTCPDCPSPIDLSNPSALEAATESLAKFNSKSPSKKYELVKVTKAMN 198 NCTLRPVSKRKTHTTCPDCPSPIDLSNPSALEAATESLAKFNSKSPSKKYELVKVTKAMN Sbjct: 121 NCTLRPVSKRKTHTTCPDCPSPIDLSNPSALEAATESLAKFNSKSPSKKYELVKVTKAMN 180 Query: 199 QWVSGPAYYVEYLIKEAPCTKSQASCSLQHSDSEPVGICQGSTVQSSLRHVPLIQPVEKS 258 QWVSGPAYYVEYLIKEAPCTKSQASCSLQHSDSEPVGICQGSTVQSSLRHVPLIQPVEKS Sbjct: 181 QWVSGPAYYVEYLIKEAPCTKSQASCSLQHSDSEPVGICQGSTVQSSLRHVPLIQPVEKS 240 Query: 259 VTVTCEFFESQAQVPGDENPAVTQGPQKLPQKNTAPTSSPSVTAPRGSIQHLPELDDEKP 318 VTVTCEFFESQAQVPGDENPAVTQGPQKLPQKNTAPTSSPSVTAPRGSIQHLPELDDEKP Sbjct: 241 VTVTCEFFESQAQVPGDENPAVTQGPQKLPQKNTAPTSSPSVTAPRGSIQHLPELDDEKP 300 Query: 319 EESKGGSPEEAFPVQLDLTTNPQGDTLDVSFLYLEPGDKKLVVLPFPGKEQRSAECPGPE 378 EESKGGSPEEAFPVQLDLTTNPQGDTLDVSFLYLEPGDKKLVVLPFPGKEQRSAECPGPE Sbjct: 301 EESKGGSPEEAFPVQLDLTTNPQGDTLDVSFLYLEPGDKKLVVLPFPGKEQRSAECPGPE 360 Query: 379 KENNPLVLPP 388 KENNPLVLPP Sbjct: 361 KENNPLVLPP 370 >2CH9-A nrPDB Length = 131 Score = 39.7 bits (91), Expect = 0.002, Method: Composition-based stats. Identities = 31/110 (28%), Positives = 51/110 (46%), Gaps = 9/110 (8%) Query: 155 PDCPSPIDLSNPSALEAATESLAKFNSKSPSK-KYELVKVTKAMNQWVSGPAYYVEYLIK 213 P P I ++P L+AA S+ KFN+ + ++ ++T+A+ Q V G Y +E I Sbjct: 17 PGFPKTIKTNDPGVLQAARYSVEKFNNCTNDMFLFKESRITRALVQIVKGLKYMLEVEIG 76 Query: 214 EAPCTKSQA----SCSLQHSDSEPVGICQGSTVQSSLRHVPLIQPVEKSV 259 C K+Q C Q + + + Q + S + VP +Q E V Sbjct: 77 RTTCKKNQHLRLDDCDFQTNHT----LKQTLSCYSEVWVVPWLQHFEVPV 122 >1CEW-I nrPDB Length = 108 Score = 34.3 bits (77), Expect = 0.088, Method: Composition-based stats. Identities = 24/79 (30%), Positives = 36/79 (45%), Gaps = 5/79 (6%) Query: 158 PSPIDLSNPSALEAATESLAKFNSKSPSK-KYELVKVTKAMNQWVSGPAYYVEYLIKEAP 216 P P+D ++ A ++A++N S K +V+V A Q VSG Y ++ I Sbjct: 3 PVPVDENDEGLQRALQFAMAEYNRASNDKYSSRVVRVISAKRQLVSGIKYILQVEIGRTT 62 Query: 217 CTKSQA---SCSLQHSDSE 232 C KS SC H + E Sbjct: 63 CPKSSGDLQSCEF-HDEPE 80 >4Z6Y-B nrPDB Length = 273 Score = 32.0 bits (71), Expect = 0.42, Method: Composition-based stats. Identities = 25/102 (24%), Positives = 44/102 (43%), Gaps = 13/102 (12%) Query: 26 LPQRPLSPLHPLGCNDSEVLAVAGFALQNINRDQKDGYMLSLNRVHDVREHYQEDMGSL- 84 LP+ P PL P D EV A++ + D D Y ++ V+ + Y++ + L Sbjct: 99 LPRSPSFPLEP----DDEVFLAIAKAMEEMVEDSVDCYWITRRFVNQLNTKYRDSLPQLP 154 Query: 85 ----FYLTLDVLETDCHVLSRKAQKDCKPRMFYESVYGQCKA 122 YL L+ D +L+ P++ Y+ + +C A Sbjct: 155 KAFEQYLNLE----DGRLLTHLRMCSAAPKLPYDLWFKRCFA 192 >4IT7-A nrPDB Length = 113 Score = 31.6 bits (70), Expect = 0.68, Method: Composition-based stats. Identities = 20/66 (30%), Positives = 33/66 (50%), Gaps = 1/66 (1%) Query: 159 SPIDLSNPSALEAATESLAKFNSKSPSK-KYELVKVTKAMNQWVSGPAYYVEYLIKEAPC 217 S D+++P A ++L + N+ S + +VKV A Q V+G +E LI C Sbjct: 10 STKDVNDPKIQALAGKALQRINAASNDLFQQTIVKVISAKTQVVAGTNTVLELLIAPTSC 69 Query: 218 TKSQAS 223 K++ S Sbjct: 70 RKNETS 75 >2IG2-H nrPDB Length = 455 Score = 30.8 bits (68), Expect = 1.1, Method: Composition-based stats. Identities = 14/28 (50%), Positives = 16/28 (57%), Gaps = 1/28 (3%) Query: 142 LRPVSKRKTHTTCPDCPSPIDLSNPSAL 169 + P S KTHT CP CP+P L PS Sbjct: 224 VEPKSCDKTHT-CPPCPAPELLGGPSVF 250 >1ZUN-B nrPDB Length = 434 Score = 30.4 bits (67), Expect = 1.3, Method: Composition-based stats. Identities = 16/55 (29%), Positives = 24/55 (43%) Query: 315 DEKPEESKGGSPEEAFPVQLDLTTNPQGDTLDVSFLYLEPGDKKLVVLPFPGKEQ 369 D K + G + A V QG T+DV++ Y +K ++ PG EQ Sbjct: 62 DSKKSGTTGDDVDLALLVDGLQAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQ 116 >1HZH-H nrPDB Length = 457 Score = 30.4 bits (67), Expect = 1.5, Method: Composition-based stats. Identities = 14/26 (53%), Positives = 15/26 (57%), Gaps = 1/26 (3%) Query: 144 PVSKRKTHTTCPDCPSPIDLSNPSAL 169 P S KTHT CP CP+P L PS Sbjct: 227 PKSCDKTHT-CPPCPAPELLGGPSVF 251 >1BRY-Y nrPDB Length = 248 Score = 29.7 bits (65), Expect = 2.2, Method: Composition-based stats. Identities = 14/31 (45%), Positives = 17/31 (54%) Query: 357 KKLVVLPFPGKEQRSAECPGPEKENNPLVLP 387 KK V LP+ G +R G +EN PL LP Sbjct: 101 KKKVTLPYSGNYERLQTAAGKIRENIPLGLP 131 >1YPX-A nrPDB Length = 375 Score = 28.9 bits (63), Expect = 3.9, Method: Composition-based stats. Identities = 24/84 (28%), Positives = 36/84 (42%), Gaps = 12/84 (14%) Query: 232 EPVGICQGSTVQSSLRHVPLIQPVEKSVTVTCEFFESQAQVPGDENPAVTQGPQKLPQKN 291 + G Q S VQ+ V + P++ + E F + GD + A K Sbjct: 86 DAAGGIQFSKVQTKSHSVKITGPIDFTTHPFIEDFIFLKEAVGDNHVA----------KQ 135 Query: 292 TAPTSSPSVTAPRGSIQHLPELDD 315 T P SP++ RG I++ P LDD Sbjct: 136 TIP--SPAMLHYRGDIEYQPYLDD 157 >1ROA-A nrPDB Length = 122 Score = 28.1 bits (61), Expect = 6.3, Method: Composition-based stats. Identities = 18/62 (29%), Positives = 28/62 (45%), Gaps = 2/62 (3%) Query: 162 DLSNPSALEAATESLAKFNSKSPSKKY--ELVKVTKAMNQWVSGPAYYVEYLIKEAPCTK 219 DL++ S A +++++N +Y ++V A Q V G YY CTK Sbjct: 18 DLNDKSVQRALDFAISEYNKVINKDEYYSRPLQVMAAYQQIVGGVNYYFNVKFGRTTCTK 77 Query: 220 SQ 221 SQ Sbjct: 78 SQ 79 >5IJO-H nrPDB Length = 522 Score = 27.7 bits (60), Expect = 9.9, Method: Composition-based stats. Identities = 22/85 (25%), Positives = 29/85 (34%) Query: 215 APCTKSQASCSLQHSDSEPVGICQGSTVQSSLRHVPLIQPVEKSVTVTCEFFESQAQVPG 274 AP T S S ++P G GS S+ P P + +Q P Sbjct: 150 APATTSGGFSFTGGSTAQPSGFNIGSAGNSAQPTAPATLPFTPATPAATTAGATQPAAPT 209 Query: 275 DENPAVTQGPQKLPQKNTAPTSSPS 299 + GP TAPTSS + Sbjct: 210 PTATITSTGPSLFASIATAPTSSAT 234 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.132 0.400 Lambda K H 0.267 0.0407 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 16,047,403 Number of Sequences: 36641 Number of extensions: 661466 Number of successful extensions: 1094 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 20 Number of HSP's that attempted gapping in prelim test: 1081 Number of HSP's gapped (non-prelim): 36 length of query: 388 length of database: 10,336,785 effective HSP length: 99 effective length of query: 289 effective length of database: 6,709,326 effective search space: 1938995214 effective search space used: 1938995214 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 60 (27.7 bits)