BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9P0R6 (139 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1SGO-A nrPDB 288 7e-79 6ASO-A nrPDB 30 0.26 3BPV-A nrPDB 27 3.7 5Y6P-a2 nrPDB 26 4.7 2P3E-A nrPDB 26 6.1 >1SGO-A nrPDB Length = 139 Score = 288 bits (736), Expect = 7e-79, Method: Compositional matrix adjust. Identities = 139/139 (100%), Positives = 139/139 (100%) Query: 1 METDCNPMELSSMSGFEEGSELNGFEGTDMKDMRLEAEAVVNDVLFAVNNMFVSKSLRCA 60 METDCNPMELSSMSGFEEGSELNGFEGTDMKDMRLEAEAVVNDVLFAVNNMFVSKSLRCA Sbjct: 1 METDCNPMELSSMSGFEEGSELNGFEGTDMKDMRLEAEAVVNDVLFAVNNMFVSKSLRCA 60 Query: 61 DDVAYINVETKERNRYCLELTEAGLKVVGYAFDQVDDHLQTPYHETVYSLLDTLSPAYRE 120 DDVAYINVETKERNRYCLELTEAGLKVVGYAFDQVDDHLQTPYHETVYSLLDTLSPAYRE Sbjct: 61 DDVAYINVETKERNRYCLELTEAGLKVVGYAFDQVDDHLQTPYHETVYSLLDTLSPAYRE 120 Query: 121 AFGNALLQRLEALKRDGQS 139 AFGNALLQRLEALKRDGQS Sbjct: 121 AFGNALLQRLEALKRDGQS 139 >6ASO-A nrPDB Length = 424 Score = 30.4 bits (67), Expect = 0.26, Method: Composition-based stats. Identities = 15/29 (51%), Positives = 19/29 (65%), Gaps = 1/29 (3%) Query: 64 AYINVETKERNRYCLELTEAGLKVVGYAF 92 AYI+V +KE RYC+E GLK+ GY Sbjct: 135 AYIDVTSKEDARYCVEKLN-GLKIEGYTL 162 >3BPV-A nrPDB Length = 138 Score = 26.6 bits (57), Expect = 3.7, Method: Compositional matrix adjust. Identities = 20/79 (25%), Positives = 37/79 (46%), Gaps = 9/79 (11%) Query: 19 GSELNGFEGTDMKD---MRLEAEAVVND----VLFAVNNMFVSKSLRCADDVAYINVE-- 69 G EL TD + +R+ E + F V+ ++++LR ++ +I E Sbjct: 19 GRELGHLNLTDAQVACLLRIHREPGIKQDELATFFHVDKGTIARTLRRLEESGFIEREQD 78 Query: 70 TKERNRYCLELTEAGLKVV 88 + R RY LE+T G +++ Sbjct: 79 PENRRRYILEVTRRGEEII 97 >5Y6P-a2 nrPDB Length = 885 Score = 26.2 bits (56), Expect = 4.7, Method: Composition-based stats. Identities = 11/40 (27%), Positives = 22/40 (55%), Gaps = 2/40 (5%) Query: 90 YAFDQVDDHLQTPYHETVYSLLDTL--SPAYREAFGNALL 127 Y ++D + Y E Y+++D + S Y E FG++++ Sbjct: 602 YGRQEIDQYFNIVYKEGYYTMIDRIIDSREYTETFGSSIV 641 >2P3E-A nrPDB Length = 420 Score = 25.8 bits (55), Expect = 6.1, Method: Composition-based stats. Identities = 17/57 (29%), Positives = 28/57 (49%), Gaps = 1/57 (1%) Query: 71 KERNRYCLELTEAGLKVVGYAFDQVDDHLQTPYHETVYSLLDTLSPAYREAFGNALL 127 KE N Y LE + L + G + ++ TP + + + AYR+AF +AL+ Sbjct: 5 KEYNPY-LEYRDGELFIEGVSLKELAQTFGTPLYVYSSNFIKERFEAYRKAFPDALI 60 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1SGO-A nrPDB 293 1e-80 Sequences not found previously or not previously below threshold: 6ASO-A nrPDB 32 0.100 5Y6P-a2 nrPDB 27 2.6 2P3E-A nrPDB 27 3.4 3BPV-A nrPDB 27 3.6 4E51-A nrPDB 25 7.9 3MH6-A nrPDB 25 8.7 CONVERGED! >1SGO-A nrPDB Length = 139 Score = 293 bits (751), Expect = 1e-80, Method: Composition-based stats. Identities = 139/139 (100%), Positives = 139/139 (100%) Query: 1 METDCNPMELSSMSGFEEGSELNGFEGTDMKDMRLEAEAVVNDVLFAVNNMFVSKSLRCA 60 METDCNPMELSSMSGFEEGSELNGFEGTDMKDMRLEAEAVVNDVLFAVNNMFVSKSLRCA Sbjct: 1 METDCNPMELSSMSGFEEGSELNGFEGTDMKDMRLEAEAVVNDVLFAVNNMFVSKSLRCA 60 Query: 61 DDVAYINVETKERNRYCLELTEAGLKVVGYAFDQVDDHLQTPYHETVYSLLDTLSPAYRE 120 DDVAYINVETKERNRYCLELTEAGLKVVGYAFDQVDDHLQTPYHETVYSLLDTLSPAYRE Sbjct: 61 DDVAYINVETKERNRYCLELTEAGLKVVGYAFDQVDDHLQTPYHETVYSLLDTLSPAYRE 120 Query: 121 AFGNALLQRLEALKRDGQS 139 AFGNALLQRLEALKRDGQS Sbjct: 121 AFGNALLQRLEALKRDGQS 139 >6ASO-A nrPDB Length = 424 Score = 32.0 bits (71), Expect = 0.100, Method: Composition-based stats. Identities = 15/29 (51%), Positives = 19/29 (65%), Gaps = 1/29 (3%) Query: 64 AYINVETKERNRYCLELTEAGLKVVGYAF 92 AYI+V +KE RYC+E GLK+ GY Sbjct: 135 AYIDVTSKEDARYCVEKLN-GLKIEGYTL 162 >5Y6P-a2 nrPDB Length = 885 Score = 27.0 bits (58), Expect = 2.6, Method: Composition-based stats. Identities = 11/40 (27%), Positives = 22/40 (55%), Gaps = 2/40 (5%) Query: 90 YAFDQVDDHLQTPYHETVYSLLDTL--SPAYREAFGNALL 127 Y ++D + Y E Y+++D + S Y E FG++++ Sbjct: 602 YGRQEIDQYFNIVYKEGYYTMIDRIIDSREYTETFGSSIV 641 >2P3E-A nrPDB Length = 420 Score = 26.6 bits (57), Expect = 3.4, Method: Composition-based stats. Identities = 17/57 (29%), Positives = 28/57 (49%), Gaps = 1/57 (1%) Query: 71 KERNRYCLELTEAGLKVVGYAFDQVDDHLQTPYHETVYSLLDTLSPAYREAFGNALL 127 KE N Y LE + L + G + ++ TP + + + AYR+AF +AL+ Sbjct: 5 KEYNPY-LEYRDGELFIEGVSLKELAQTFGTPLYVYSSNFIKERFEAYRKAFPDALI 60 >3BPV-A nrPDB Length = 138 Score = 26.6 bits (57), Expect = 3.6, Method: Composition-based stats. Identities = 13/46 (28%), Positives = 26/46 (56%), Gaps = 2/46 (4%) Query: 45 LFAVNNMFVSKSLRCADDVAYINVET--KERNRYCLELTEAGLKVV 88 F V+ ++++LR ++ +I E + R RY LE+T G +++ Sbjct: 52 FFHVDKGTIARTLRRLEESGFIEREQDPENRRRYILEVTRRGEEII 97 >4E51-A nrPDB Length = 467 Score = 25.4 bits (54), Expect = 7.9, Method: Composition-based stats. Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 4/55 (7%) Query: 71 KERNRYCLELTEAGLKVVGYAFDQVDDHLQTPYHETVYSLLDTLSPAYREAFGNA 125 +ER + +EL +K + D++DD Q + +LDT +PA +E NA Sbjct: 199 EERAAHRVEL----IKYLEQHADKLDDDAQRRLYTNPLRVLDTKNPALQEIVRNA 249 >3MH6-A nrPDB Length = 456 Score = 25.4 bits (54), Expect = 8.7, Method: Composition-based stats. Identities = 12/37 (32%), Positives = 20/37 (54%) Query: 8 MELSSMSGFEEGSELNGFEGTDMKDMRLEAEAVVNDV 44 ++ SS + + S NG EG +M + + VVN+V Sbjct: 354 LQQSSQNQVDSSSIFNGIEGAEMSNKGKDQGVVVNNV 390 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.132 0.373 Lambda K H 0.267 0.0406 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,298,639 Number of Sequences: 36641 Number of extensions: 191783 Number of successful extensions: 505 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 498 Number of HSP's gapped (non-prelim): 17 length of query: 139 length of database: 10,336,785 effective HSP length: 87 effective length of query: 52 effective length of database: 7,149,018 effective search space: 371748936 effective search space used: 371748936 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 54 (25.4 bits)