BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9NYA1 (384 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4L02-A nrPDB 718 0.0 3T5P-A nrPDB 48 6e-06 4WER-A nrPDB 47 2e-05 2QVL-A nrPDB 38 0.007 >4L02-A nrPDB Length = 384 Score = 718 bits (1854), Expect = 0.0, Method: Compositional matrix adjust. Identities = 356/356 (100%), Positives = 356/356 (100%) Query: 9 GVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEE 68 GVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEE Sbjct: 29 GVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEE 88 Query: 69 LGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQ 128 LGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQ Sbjct: 89 LGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQ 148 Query: 129 VTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLG 188 VTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLG Sbjct: 149 VTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLG 208 Query: 189 EMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSH 248 EMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSH Sbjct: 209 EMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSH 268 Query: 249 WTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEK 308 WTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEK Sbjct: 269 WTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEK 328 Query: 309 GRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSG 364 GRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSG Sbjct: 329 GRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSG 384 >3T5P-A nrPDB Length = 306 Score = 48.1 bits (113), Expect = 6e-06, Method: Compositional matrix adjust. Identities = 44/199 (22%), Positives = 92/199 (46%), Gaps = 23/199 (11%) Query: 16 RVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDAL 75 +VL+++NP+ G+G L + + P LA A ++ T+ + A + + E + D + Sbjct: 14 KVLLIVNPKAGQGD-LHTNLTKIVPPLAAAFPDLHILHTKEQGDATKYCQ-EFASKVDLI 71 Query: 76 VVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLL 135 +V GDG + E NGL I+ L +P G+ N + +L G Q E Sbjct: 72 IVFGGDGTVFECTNGLA-----PLEIRPTLAIIPGGTCNDFSRTL----GVPQNIAE--- 119 Query: 136 TNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADV--DLESEKYRRLGEMRFT 193 L+ + + P+++ + L + + G +++V ++++E+ +LG++ + Sbjct: 120 --AAKLITKEHVKPVDVAKANGQHFLNFWGI-----GLVSEVSNNIDAEEKAKLGKIGYY 172 Query: 194 LGTFLRLAALRTYRGRLAY 212 L T + T+ ++ Y Sbjct: 173 LSTIRTVKNAETFPVKITY 191 >4WER-A nrPDB Length = 303 Score = 46.6 bits (109), Expect = 2e-05, Method: Compositional matrix adjust. Identities = 46/193 (23%), Positives = 86/193 (44%), Gaps = 22/193 (11%) Query: 16 RVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDAL 75 + ++++NP G KA + F + + L + + TE+ A + R +D++ Sbjct: 6 KAVLIVNPSSGGEKAKE-FETLAEEKLKQLFDEVVVKQTEKGGDAEQFAREAAESHFDSV 64 Query: 76 VVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLL 135 VM GDG ++E ++GL E+ A + P G+ N LA +LN E+ + L Sbjct: 65 FVMGGDGTVNEGISGLAEQ-----AYRPKFGFFPLGTVNDLARALNLPMDPEEAIQQLDL 119 Query: 136 TNCTLLLCRRLLSP--MNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFT 193 + L ++ MN++++ T I DVD+E + +LG++ + Sbjct: 120 EKTSALDVGKINDDYFMNVVAIGTIP------------ESINDVDVEQK--TKLGKLAYF 165 Query: 194 LGTFLRLAALRTY 206 + LA +TY Sbjct: 166 ISGAKHLANAQTY 178 >2QVL-A nrPDB Length = 337 Score = 38.1 bits (87), Expect = 0.007, Method: Compositional matrix adjust. Identities = 31/117 (26%), Positives = 51/117 (43%), Gaps = 12/117 (10%) Query: 7 PRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEIS---FTLMLTERRNHAREL 63 PRG R ++ NP GK + F+ + L + E + + TE+ A Sbjct: 17 PRGSHMMRKRARIIYNPTSGK----EQFKRELPDALIKLEKAGYETSAYATEKIGDATLE 72 Query: 64 VRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASL 120 +D L+ GDG ++EVVNG+ E+P+ + L +P G+ N +L Sbjct: 73 AERAMHENYDVLIAAGGDGTLNEVVNGIAEKPN-----RPKLGVIPMGTVNDFGRAL 124 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4L02-A nrPDB 620 e-178 3T5P-A nrPDB 224 7e-59 4WER-A nrPDB 217 6e-57 Sequences not found previously or not previously below threshold: 2QVL-A nrPDB 94 1e-19 2P1R-A nrPDB 67 2e-11 2JGR-A nrPDB 65 3e-11 6I04-A nrPDB 33 0.18 2UZX-B nrPDB 33 0.18 6OIX-A nrPDB 28 9.0 >4L02-A nrPDB Length = 384 Score = 620 bits (1598), Expect = e-178, Method: Composition-based stats. Identities = 356/356 (100%), Positives = 356/356 (100%) Query: 9 GVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEE 68 GVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEE Sbjct: 29 GVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEE 88 Query: 69 LGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQ 128 LGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQ Sbjct: 89 LGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQ 148 Query: 129 VTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLG 188 VTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLG Sbjct: 149 VTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLG 208 Query: 189 EMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSH 248 EMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSH Sbjct: 209 EMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSH 268 Query: 249 WTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEK 308 WTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEK Sbjct: 269 WTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEK 328 Query: 309 GRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSG 364 GRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSG Sbjct: 329 GRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSG 384 >3T5P-A nrPDB Length = 306 Score = 224 bits (570), Expect = 7e-59, Method: Composition-based stats. Identities = 44/199 (22%), Positives = 92/199 (46%), Gaps = 23/199 (11%) Query: 16 RVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDAL 75 +VL+++NP+ G+G L + + P LA A ++ T+ + A + + E + D + Sbjct: 14 KVLLIVNPKAGQGD-LHTNLTKIVPPLAAAFPDLHILHTKEQGDATKYCQ-EFASKVDLI 71 Query: 76 VVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLL 135 +V GDG + E NGL I+ L +P G+ N + +L G Q E Sbjct: 72 IVFGGDGTVFECTNGLA-----PLEIRPTLAIIPGGTCNDFSRTL----GVPQNIAE--- 119 Query: 136 TNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADV--DLESEKYRRLGEMRFT 193 L+ + + P+++ + L + + G +++V ++++E+ +LG++ + Sbjct: 120 --AAKLITKEHVKPVDVAKANGQHFLNFWGI-----GLVSEVSNNIDAEEKAKLGKIGYY 172 Query: 194 LGTFLRLAALRTYRGRLAY 212 L T + T+ ++ Y Sbjct: 173 LSTIRTVKNAETFPVKITY 191 >4WER-A nrPDB Length = 303 Score = 217 bits (553), Expect = 6e-57, Method: Composition-based stats. Identities = 47/198 (23%), Positives = 88/198 (44%), Gaps = 22/198 (11%) Query: 16 RVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDAL 75 + ++++NP G KA + F + + L + + TE+ A + R +D++ Sbjct: 6 KAVLIVNPSSGGEKAKE-FETLAEEKLKQLFDEVVVKQTEKGGDAEQFAREAAESHFDSV 64 Query: 76 VVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLL 135 VM GDG ++E ++GL E+ A + P G+ N LA +LN E+ + L Sbjct: 65 FVMGGDGTVNEGISGLAEQ-----AYRPKFGFFPLGTVNDLARALNLPMDPEEAIQQLDL 119 Query: 136 TNCTLLLCRRLLSP--MNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFT 193 + L ++ MN++++ T I DVD+E + +LG++ + Sbjct: 120 EKTSALDVGKINDDYFMNVVAIGTIP------------ESINDVDVEQK--TKLGKLAYF 165 Query: 194 LGTFLRLAALRTYRGRLA 211 + LA +TY L+ Sbjct: 166 ISGAKHLANAQTYPFHLS 183 >2QVL-A nrPDB Length = 337 Score = 93.6 bits (231), Expect = 1e-19, Method: Composition-based stats. Identities = 40/208 (19%), Positives = 76/208 (36%), Gaps = 22/208 (10%) Query: 7 PRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRS 66 PRG R ++ NP GK + + L +A + TE+ A Sbjct: 17 PRGSHMMRKRARIIYNPTSGKEQFKRE-LPDALIKLEKAGYETSAYATEKIGDATLEAER 75 Query: 67 EELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGY 126 +D L+ GDG ++EVVNG+ E+P+ + L +P G+ N +L+ Sbjct: 76 AMHENYDVLIAAGGDGTLNEVVNGIAEKPN-----RPKLGVIPMGTVNDFGRALHIPNDI 130 Query: 127 EQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRR 186 + + + T + ++ + + A G + V E+ + Sbjct: 131 MGALDVIIEGHSTKVDIGKMN--------------NRYFINLAAGGQLTQVSYETPSKLK 176 Query: 187 --LGEMRFTLGTFLRLAALRTYRGRLAY 212 +G + + F L ++ R+ Y Sbjct: 177 SIVGPFAYYIKGFEMLPQMKAVDLRIEY 204 >2P1R-A nrPDB Length = 299 Score = 66.6 bits (161), Expect = 2e-11, Method: Composition-based stats. Identities = 32/193 (16%), Positives = 67/193 (34%), Gaps = 18/193 (9%) Query: 18 LVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVV 77 L++LN + + L+ LL + I + +T + A+ V + ++ Sbjct: 8 LLILNGKSADNQPLR----EAITLLRDEGIQIHVRVTWEKGDAQRYVDEARRLGVETVIA 63 Query: 78 MSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTN 137 GDG ++EV L++ D + L LP G+ N A S ++ + N Sbjct: 64 GGGDGTINEVSTALIQIRD---GVAPALGLLPLGTANDFATSAGIPEALDKALKLAIAGN 120 Query: 138 CTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTF 197 + + +++ T G + + LG + + + Sbjct: 121 AMEIDMAMVNDKTCFINMATGGF-----------GTRITTETPEKLKAALGGVSYLIHGL 169 Query: 198 LRLAALRTYRGRL 210 +R+ L R + Sbjct: 170 MRMDTLTPDRCEI 182 >2JGR-A nrPDB Length = 299 Score = 65.5 bits (158), Expect = 3e-11, Method: Composition-based stats. Identities = 30/193 (15%), Positives = 67/193 (34%), Gaps = 18/193 (9%) Query: 18 LVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVV 77 L++LN + L+ LL E ++ + +T + A V ++ Sbjct: 8 LLILNGKSTDNLPLR----EAIMLLREEGMTIHVRVTWEKGDAARYVEEARKFGVATVIA 63 Query: 78 MSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTN 137 GDG ++EV L++ + L LP G+ N A S+ ++ + + Sbjct: 64 GGGDGTINEVSTALIQCEGDDI---PALGILPLGTANDFATSVGIPEALDKALKLAIAGD 120 Query: 138 CTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTF 197 + ++ +++ T G + + LG + + + Sbjct: 121 AIAIDMAQVNKQTCFINMATGGF-----------GTRITTETPEKLKAALGSVSYIIHGL 169 Query: 198 LRLAALRTYRGRL 210 +R+ L+ R + Sbjct: 170 MRMDTLQPDRCEI 182 >6I04-A nrPDB Length = 552 Score = 33.1 bits (74), Expect = 0.18, Method: Composition-based stats. Identities = 31/149 (20%), Positives = 48/149 (32%), Gaps = 27/149 (18%) Query: 102 QKPLCSLPAGSGNAL---------AASLNHYAGY--EQVTNEDLLTNCTLLLCRRLLSPM 150 + +C+ P N L H+ G E N LL N + RR Sbjct: 335 RSAMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARR----- 389 Query: 151 NLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRL 210 + + L G ++V L S + + L +A L T GR Sbjct: 390 ---DEYRTEFTTALQRVDLFMGQFSEVLLTS--------ISTFIKGDLTIANLGTSEGRF 438 Query: 211 AYLPVGRVGSKTPASPVVVQQGPVDAHLV 239 + V R G TP ++ PV ++ Sbjct: 439 MQVVVSRSGPSTPHVNFLLDSHPVSPEVI 467 >2UZX-B nrPDB Length = 727 Score = 33.1 bits (74), Expect = 0.18, Method: Composition-based stats. Identities = 31/149 (20%), Positives = 48/149 (32%), Gaps = 27/149 (18%) Query: 102 QKPLCSLPAGSGNAL---------AASLNHYAGY--EQVTNEDLLTNCTLLLCRRLLSPM 150 + +C+ P N L H+ G E N LL N + RR Sbjct: 338 RSAMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARR----- 392 Query: 151 NLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRL 210 + + L G ++V L S + + L +A L T GR Sbjct: 393 ---DEYRTEFTTALQRVDLFMGQFSEVLLTS--------ISTFIKGDLTIANLGTSEGRF 441 Query: 211 AYLPVGRVGSKTPASPVVVQQGPVDAHLV 239 + V R G TP ++ PV ++ Sbjct: 442 MQVVVSRSGPSTPHVNFLLDSHPVSPEVI 470 >6OIX-A nrPDB Length = 505 Score = 27.7 bits (60), Expect = 9.0, Method: Composition-based stats. Identities = 22/122 (18%), Positives = 36/122 (29%), Gaps = 7/122 (5%) Query: 35 RSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMER 94 ++ V PL A + L + + E L R L ++ GL + + G E Sbjct: 51 KTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGL--DELTGPFES 108 Query: 95 PDWETAI-----QKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSP 149 + + P + N H E D + L R P Sbjct: 109 IVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAALRLRDGEEP 168 Query: 150 MN 151 +N Sbjct: 169 LN 170 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.320 0.132 0.375 Lambda K H 0.267 0.0404 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 15,281,247 Number of Sequences: 36641 Number of extensions: 621882 Number of successful extensions: 1489 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 3 Number of HSP's that attempted gapping in prelim test: 1462 Number of HSP's gapped (non-prelim): 17 length of query: 384 length of database: 10,336,785 effective HSP length: 99 effective length of query: 285 effective length of database: 6,709,326 effective search space: 1912157910 effective search space used: 1912157910 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 60 (27.7 bits)