BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9NUN7 (267 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6G7O-A nrPDB 506 e-144 1RKX-A nrPDB 34 0.076 1WVG-A nrPDB 29 2.2 5L43-A nrPDB 27 7.9 >6G7O-A nrPDB Length = 350 Score = 506 bits (1304), Expect = e-144, Method: Compositional matrix adjust. Identities = 243/243 (100%), Positives = 243/243 (100%) Query: 2 APAADREGYWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMIIPPMFGAVQSVRDGLEK 61 APAADREGYWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMIIPPMFGAVQSVRDGLEK Sbjct: 2 APAADREGYWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMIIPPMFGAVQSVRDGLEK 61 Query: 62 RYIASYLALTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKIKNSVNYHL 121 RYIASYLALTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKIKNSVNYHL Sbjct: 62 RYIASYLALTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKIKNSVNYHL 121 Query: 122 LFTLVLFSLIVTTVYLKVKEPIFHQVMYGMLVFTLVLRSIYIVTWVYPWLRGLGYTSLGI 181 LFTLVLFSLIVTTVYLKVKEPIFHQVMYGMLVFTLVLRSIYIVTWVYPWLRGLGYTSLGI Sbjct: 122 LFTLVLFSLIVTTVYLKVKEPIFHQVMYGMLVFTLVLRSIYIVTWVYPWLRGLGYTSLGI 181 Query: 182 FLLGFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLHILFSLYTRTL 241 FLLGFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLHILFSLYTRTL Sbjct: 182 FLLGFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLHILFSLYTRTL 241 Query: 242 YLR 244 YLR Sbjct: 242 YLR 244 >1RKX-A nrPDB Length = 357 Score = 33.9 bits (76), Expect = 0.076, Method: Compositional matrix adjust. Identities = 19/56 (33%), Positives = 26/56 (46%) Query: 183 LLGFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLHILFSLYT 238 ++G W +D I + LR F + P II W H+L L YL + LYT Sbjct: 196 VIGGGDWALDRIVPDILRAFEQSQPVIIRNPHAIRPWQHVLEPLSGYLLLAQKLYT 251 >1WVG-A nrPDB Length = 359 Score = 28.9 bits (63), Expect = 2.2, Method: Compositional matrix adjust. Identities = 19/68 (27%), Positives = 29/68 (42%), Gaps = 1/68 (1%) Query: 173 GLGYTSL-GIFLLGFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLH 231 G+G S+ ++G W D + + LR+F II W H+L L Y+ Sbjct: 185 GVGLASVRAGNVIGGGDWAKDRLIPDILRSFENNQQVIIRNPYSIRPWQHVLEPLSGYIV 244 Query: 232 ILFSLYTR 239 + LYT Sbjct: 245 VAQRLYTE 252 >5L43-A nrPDB Length = 683 Score = 27.3 bits (59), Expect = 7.9, Method: Compositional matrix adjust. Identities = 15/53 (28%), Positives = 28/53 (52%), Gaps = 6/53 (11%) Query: 212 ITTQFHAWWHILTGLGSYLHILFSLYTRTL----YLRYRPKVKFLFGIWPVIL 260 + T FH + H L GL S +H + ++ T ++ Y +V ++ +WP +L Sbjct: 470 VNTMFHEFGHALHGLFSEVH--YPRFSGTAVPRDFVEYPSQVNEMWAVWPSVL 520 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6G7O-A nrPDB 496 e-141 Sequences not found previously or not previously below threshold: 1RKX-A nrPDB 34 0.079 5L43-A nrPDB 28 4.9 1WVG-A nrPDB 28 5.8 CONVERGED! >6G7O-A nrPDB Length = 350 Score = 496 bits (1278), Expect = e-141, Method: Composition-based stats. Identities = 243/243 (100%), Positives = 243/243 (100%) Query: 2 APAADREGYWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMIIPPMFGAVQSVRDGLEK 61 APAADREGYWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMIIPPMFGAVQSVRDGLEK Sbjct: 2 APAADREGYWGPTTSTLDWCEENYSVTWYIAEFWNTVSNLIMIIPPMFGAVQSVRDGLEK 61 Query: 62 RYIASYLALTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKIKNSVNYHL 121 RYIASYLALTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKIKNSVNYHL Sbjct: 62 RYIASYLALTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCIFVYCMFECFKIKNSVNYHL 121 Query: 122 LFTLVLFSLIVTTVYLKVKEPIFHQVMYGMLVFTLVLRSIYIVTWVYPWLRGLGYTSLGI 181 LFTLVLFSLIVTTVYLKVKEPIFHQVMYGMLVFTLVLRSIYIVTWVYPWLRGLGYTSLGI Sbjct: 122 LFTLVLFSLIVTTVYLKVKEPIFHQVMYGMLVFTLVLRSIYIVTWVYPWLRGLGYTSLGI 181 Query: 182 FLLGFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLHILFSLYTRTL 241 FLLGFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLHILFSLYTRTL Sbjct: 182 FLLGFLFWNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLHILFSLYTRTL 241 Query: 242 YLR 244 YLR Sbjct: 242 YLR 244 >1RKX-A nrPDB Length = 357 Score = 33.8 bits (76), Expect = 0.079, Method: Composition-based stats. Identities = 18/50 (36%), Positives = 23/50 (46%) Query: 189 WNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLHILFSLYT 238 W +D I + LR F + P II W H+L L YL + LYT Sbjct: 202 WALDRIVPDILRAFEQSQPVIIRNPHAIRPWQHVLEPLSGYLLLAQKLYT 251 >5L43-A nrPDB Length = 683 Score = 28.0 bits (61), Expect = 4.9, Method: Composition-based stats. Identities = 15/53 (28%), Positives = 28/53 (52%), Gaps = 6/53 (11%) Query: 212 ITTQFHAWWHILTGLGSYLHILFSLYTRTL----YLRYRPKVKFLFGIWPVIL 260 + T FH + H L GL S +H + ++ T ++ Y +V ++ +WP +L Sbjct: 470 VNTMFHEFGHALHGLFSEVH--YPRFSGTAVPRDFVEYPSQVNEMWAVWPSVL 520 >1WVG-A nrPDB Length = 359 Score = 27.6 bits (60), Expect = 5.8, Method: Composition-based stats. Identities = 15/50 (30%), Positives = 21/50 (42%) Query: 189 WNIDNIFCESLRNFRKKVPPIIGITTQFHAWWHILTGLGSYLHILFSLYT 238 W D + + LR+F II W H+L L Y+ + LYT Sbjct: 202 WAKDRLIPDILRSFENNQQVIIRNPYSIRPWQHVLEPLSGYIVVAQRLYT 251 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.332 0.145 0.497 Lambda K H 0.267 0.0460 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,673,568 Number of Sequences: 36641 Number of extensions: 570575 Number of successful extensions: 1301 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 1285 Number of HSP's gapped (non-prelim): 17 length of query: 267 length of database: 10,336,785 effective HSP length: 95 effective length of query: 172 effective length of database: 6,855,890 effective search space: 1179213080 effective search space used: 1179213080 T: 11 A: 40 X1: 16 ( 7.7 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 59 (27.2 bits)