BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9NSA3 (81 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1M1E-B nrPDB 167 9e-43 1LUJ-B nrPDB 146 2e-36 6JEQ-A nrPDB 30 0.24 6CA8-A nrPDB 27 2.9 3WDH-A nrPDB 25 5.5 5B09-A nrPDB 25 6.2 1URF-A nrPDB 25 6.9 >1M1E-B nrPDB Length = 81 Score = 167 bits (423), Expect = 9e-43, Method: Compositional matrix adjust. Identities = 81/81 (100%), Positives = 81/81 (100%) Query: 1 MNREGAPGKSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVVNSQLSQLPPHSI 60 MNREGAPGKSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVVNSQLSQLPPHSI Sbjct: 1 MNREGAPGKSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVVNSQLSQLPPHSI 60 Query: 61 DQGAEDVVMAFSRSETEDRRQ 81 DQGAEDVVMAFSRSETEDRRQ Sbjct: 61 DQGAEDVVMAFSRSETEDRRQ 81 >1LUJ-B nrPDB Length = 75 Score = 146 bits (369), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 71/75 (94%), Positives = 72/75 (96%) Query: 1 MNREGAPGKSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVVNSQLSQLPPHSI 60 MNREGAP KSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVV+SQLSQLP HSI Sbjct: 1 MNREGAPAKSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVVSSQLSQLPQHSI 60 Query: 61 DQGAEDVVMAFSRSE 75 DQ AEDVVMAFSRSE Sbjct: 61 DQAAEDVVMAFSRSE 75 >6JEQ-A nrPDB Length = 675 Score = 30.0 bits (66), Expect = 0.24, Method: Composition-based stats. Identities = 17/55 (30%), Positives = 27/55 (49%) Query: 7 PGKSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVVNSQLSQLPPHSID 61 PG S EE ++ ++L G + +EF+RT GV NS S + + +D Sbjct: 483 PGASDEERRAMHRLASAIVLTSQGIPFLHAGQEFMRTKGGVENSYKSPIEVNWLD 537 >6CA8-A nrPDB Length = 751 Score = 26.6 bits (57), Expect = 2.9, Method: Composition-based stats. Identities = 13/47 (27%), Positives = 28/47 (59%), Gaps = 2/47 (4%) Query: 34 TASEEEFLRTYAGVVNSQLSQLPPHSIDQGAEDVVMAFSRSETEDRR 80 T+++ EF + ++ + N ++ L + D+ + + MAFS+ EDR+ Sbjct: 187 TSTDREFKQYFSDIKNHKIMFL--WTGDRDGDSIDMAFSKKRIEDRK 231 >3WDH-A nrPDB Length = 710 Score = 25.4 bits (54), Expect = 5.5, Method: Composition-based stats. Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 6/60 (10%) Query: 6 APGKSPEEMYIQ---QKVRVLLMLRKMGSNLTASEEEFLRTYAGVVNSQLSQLPPHSIDQ 62 A + E YI+ QK+ ++L G S +EF RT GV NS S P ++Q Sbjct: 535 AVANAHESEYIRRKRQKLATAIVLLSQGIPFLHSGQEFYRTKKGVENSYNS---PDEVNQ 591 >5B09-A nrPDB Length = 104 Score = 25.4 bits (54), Expect = 6.2, Method: Compositional matrix adjust. Identities = 11/30 (36%), Positives = 18/30 (60%) Query: 20 VRVLLMLRKMGSNLTASEEEFLRTYAGVVN 49 V+ L++L+ A +EEF +TY +VN Sbjct: 6 VKHLIVLKFKDEITEAQKEEFFKTYVNLVN 35 >1URF-A nrPDB Length = 81 Score = 25.0 bits (53), Expect = 6.9, Method: Compositional matrix adjust. Identities = 8/21 (38%), Positives = 15/21 (71%) Query: 60 IDQGAEDVVMAFSRSETEDRR 80 + QGAE+++ +S T+DR+ Sbjct: 23 VKQGAENMIQTYSNGSTKDRK 43 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1M1E-B nrPDB 146 2e-36 1LUJ-B nrPDB 130 1e-31 Sequences not found previously or not previously below threshold: 6JEQ-A nrPDB 30 0.18 5OQJ-X nrPDB 27 1.4 CONVERGED! >1M1E-B nrPDB Length = 81 Score = 146 bits (369), Expect = 2e-36, Method: Composition-based stats. Identities = 81/81 (100%), Positives = 81/81 (100%) Query: 1 MNREGAPGKSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVVNSQLSQLPPHSI 60 MNREGAPGKSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVVNSQLSQLPPHSI Sbjct: 1 MNREGAPGKSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVVNSQLSQLPPHSI 60 Query: 61 DQGAEDVVMAFSRSETEDRRQ 81 DQGAEDVVMAFSRSETEDRRQ Sbjct: 61 DQGAEDVVMAFSRSETEDRRQ 81 >1LUJ-B nrPDB Length = 75 Score = 130 bits (327), Expect = 1e-31, Method: Composition-based stats. Identities = 71/75 (94%), Positives = 72/75 (96%) Query: 1 MNREGAPGKSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVVNSQLSQLPPHSI 60 MNREGAP KSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVV+SQLSQLP HSI Sbjct: 1 MNREGAPAKSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVVSSQLSQLPQHSI 60 Query: 61 DQGAEDVVMAFSRSE 75 DQ AEDVVMAFSRSE Sbjct: 61 DQAAEDVVMAFSRSE 75 >6JEQ-A nrPDB Length = 675 Score = 30.4 bits (67), Expect = 0.18, Method: Composition-based stats. Identities = 17/55 (30%), Positives = 27/55 (49%) Query: 7 PGKSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVVNSQLSQLPPHSID 61 PG S EE ++ ++L G + +EF+RT GV NS S + + +D Sbjct: 483 PGASDEERRAMHRLASAIVLTSQGIPFLHAGQEFMRTKGGVENSYKSPIEVNWLD 537 >5OQJ-X nrPDB Length = 328 Score = 27.4 bits (59), Expect = 1.4, Method: Composition-based stats. Identities = 17/61 (27%), Positives = 27/61 (44%), Gaps = 11/61 (18%) Query: 30 GSNLTASEEEFLRTYAGVVNSQLSQLP---------PHSIDQGAEDVVMAFSRSETEDRR 80 G NL +EEF++ + V Q ++LP P S+D + R E + +R Sbjct: 249 GGNLKCIDEEFVKMWENVQLPQFAELPRKLQDLGLKPASVDPAT--IKRQTKRVEVKKKR 306 Query: 81 Q 81 Q Sbjct: 307 Q 307 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.305 0.131 0.360 Lambda K H 0.267 0.0389 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 2,573,151 Number of Sequences: 36641 Number of extensions: 70966 Number of successful extensions: 146 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 133 Number of HSP's gapped (non-prelim): 14 length of query: 81 length of database: 10,336,785 effective HSP length: 51 effective length of query: 30 effective length of database: 8,468,094 effective search space: 254042820 effective search space used: 254042820 T: 11 A: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.4 bits) S2: 52 (24.7 bits)