BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9NRX4 (125 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2OZX-A nrPDB 257 9e-70 2HW4-A nrPDB 251 6e-68 5UCY-B nrPDB 29 0.67 6GJ4-B nrPDB 28 1.0 6E88-B nrPDB 28 1.0 5IJ0-B nrPDB 28 1.4 3F7O-A nrPDB 27 1.8 6DEO-A nrPDB 27 2.1 3N29-A nrPDB 26 3.4 5GSL-A nrPDB 26 3.6 4R27-A nrPDB 26 3.9 3WBE-A nrPDB 26 4.0 3L4Y-A nrPDB 26 4.2 5A3V-A nrPDB 26 4.3 4I6M-B nrPDB 25 5.9 3WEE-A nrPDB 25 6.0 >2OZX-A nrPDB Length = 125 Score = 257 bits (656), Expect = 9e-70, Method: Compositional matrix adjust. Identities = 125/125 (100%), Positives = 125/125 (100%) Query: 1 MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKV 60 MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKV Sbjct: 1 MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKV 60 Query: 61 SGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTW 120 SGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTW Sbjct: 61 SGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTW 120 Query: 121 ANDGY 125 ANDGY Sbjct: 121 ANDGY 125 >2HW4-A nrPDB Length = 144 Score = 251 bits (641), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 121/121 (100%), Positives = 121/121 (100%) Query: 5 DLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDM 64 DLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDM Sbjct: 24 DLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDM 83 Query: 65 QKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDG 124 QKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDG Sbjct: 84 QKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDG 143 Query: 125 Y 125 Y Sbjct: 144 Y 144 >5UCY-B nrPDB Length = 429 Score = 28.9 bits (63), Expect = 0.67, Method: Composition-based stats. Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 4/40 (10%) Query: 46 GYKWAEYH----ADIYDKVSGDMQKQGCDCECLGGGRISH 81 G WA+ H A++ D V ++K+ C+CL G +I+H Sbjct: 98 GNNWAKGHYTEGAELIDSVLDVVRKEAEGCDCLQGFQITH 137 >6GJ4-B nrPDB Length = 445 Score = 28.1 bits (61), Expect = 1.0, Method: Composition-based stats. Identities = 19/74 (25%), Positives = 32/74 (43%), Gaps = 14/74 (18%) Query: 46 GYKWAEYH----ADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQ 101 G WA+ H A++ D V ++K+ C+CL G +++H G + Sbjct: 98 GNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLL------ 151 Query: 102 HAISTEKIKAKYPD 115 KI+ +YPD Sbjct: 152 ----ISKIREEYPD 161 >6E88-B nrPDB Length = 426 Score = 28.1 bits (61), Expect = 1.0, Method: Composition-based stats. Identities = 19/74 (25%), Positives = 32/74 (43%), Gaps = 14/74 (18%) Query: 46 GYKWAEYH----ADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQ 101 G WA+ H A++ D V ++K+ C+CL G +++H G + Sbjct: 98 GNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLL------ 151 Query: 102 HAISTEKIKAKYPD 115 KI+ +YPD Sbjct: 152 ----ISKIREEYPD 161 >5IJ0-B nrPDB Length = 426 Score = 27.7 bits (60), Expect = 1.4, Method: Composition-based stats. Identities = 13/40 (32%), Positives = 24/40 (60%), Gaps = 4/40 (10%) Query: 46 GYKWAEYH----ADIYDKVSGDMQKQGCDCECLGGGRISH 81 G WA+ H A++ D V ++K+ +C+CL G +++H Sbjct: 98 GNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTH 137 >3F7O-A nrPDB Length = 284 Score = 27.3 bits (59), Expect = 1.8, Method: Compositional matrix adjust. Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Query: 77 GRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYE 117 GRISH+S+ + Y S G + I T ++A +P++E Sbjct: 13 GRISHRSKGSTTYEYDTSGGSGTCAYVIDT-GVEASHPEFE 52 >6DEO-A nrPDB Length = 682 Score = 26.9 bits (58), Expect = 2.1, Method: Composition-based stats. Identities = 13/30 (43%), Positives = 17/30 (56%), Gaps = 3/30 (10%) Query: 10 PD---VDIDSDGVFKYVLIRVHSAPRSGAP 36 PD +DID D F L + SA ++GAP Sbjct: 535 PDAIVIDIDGDASFNMTLTELSSAVQAGAP 564 >3N29-A nrPDB Length = 418 Score = 26.2 bits (56), Expect = 3.4, Method: Composition-based stats. Identities = 12/33 (36%), Positives = 18/33 (54%), Gaps = 1/33 (3%) Query: 59 KVSGDMQKQGCDCECLGGGRISHQSQDKKIHVY 91 K+ G+ K GC C L + + + DK+IH Y Sbjct: 86 KIVGEYLK-GCTCSGLWEAKFAKEYMDKEIHTY 117 >5GSL-A nrPDB Length = 778 Score = 26.2 bits (56), Expect = 3.6, Method: Composition-based stats. Identities = 20/85 (23%), Positives = 38/85 (44%), Gaps = 16/85 (18%) Query: 5 DLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDM 64 ++ LIP + +D++G+ + V+ + + R K E A I DKV G + Sbjct: 569 NMRLIPYLSVDAEGIDRMVV----------------RNVAREVKGGEAIAWIGDKVVGTI 612 Query: 65 QKQGCDCECLGGGRISHQSQDKKIH 89 ++G + G R+ + S +H Sbjct: 613 VRKGKGSAVILGFRLQYYSSYHDMH 637 >4R27-A nrPDB Length = 434 Score = 26.2 bits (56), Expect = 3.9, Method: Composition-based stats. Identities = 11/21 (52%), Positives = 12/21 (57%) Query: 100 AQHAISTEKIKAKYPDYEVTW 120 A H +TE IKA PD V W Sbjct: 217 AAHKAATEAIKAHRPDLRVGW 237 >3WBE-A nrPDB Length = 489 Score = 26.2 bits (56), Expect = 4.0, Method: Composition-based stats. Identities = 20/88 (22%), Positives = 35/88 (39%), Gaps = 6/88 (6%) Query: 33 SGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYG 92 S P K+ ++ K +YH D ++ +++ GCD G + D G Sbjct: 401 SNNPFISIKDALKDSKRIKYHNDYLTNLAASIKEDGCDVR----GYFAWSLLDNWEWAAG 456 Query: 93 YSMAYGPAQHAISTEKIKAKYPDYEVTW 120 YS +G + + + +YP V W Sbjct: 457 YSSRFG--LYFVDYKDNLKRYPKNSVQW 482 >3L4Y-A nrPDB Length = 875 Score = 26.2 bits (56), Expect = 4.2, Method: Composition-based stats. Identities = 17/55 (30%), Positives = 25/55 (45%), Gaps = 1/55 (1%) Query: 71 CECLGGGRISHQSQDKKIH-VYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDG 124 C+ L + H + IH +YGYSMA A+ A + K + T+A G Sbjct: 479 CKTLCMDAVQHWGKQYDIHNLYGYSMAVATAEAAKTVFPNKRSFILTRSTFAGSG 533 >5A3V-A nrPDB Length = 329 Score = 26.2 bits (56), Expect = 4.3, Method: Compositional matrix adjust. Identities = 13/41 (31%), Positives = 23/41 (56%) Query: 74 LGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYP 114 +G G + ++ DK + V + G A+ A++TEK+ K P Sbjct: 80 VGSGVKNFKAGDKVVAVLSHLGGGGLAEFAVATEKLTVKRP 120 >4I6M-B nrPDB Length = 439 Score = 25.4 bits (54), Expect = 5.9, Method: Composition-based stats. Identities = 9/15 (60%), Positives = 9/15 (60%) Query: 111 AKYPDYEVTWANDGY 125 AKYPDY W GY Sbjct: 382 AKYPDYFPEWKKSGY 396 >3WEE-A nrPDB Length = 476 Score = 25.4 bits (54), Expect = 6.0, Method: Composition-based stats. Identities = 9/15 (60%), Positives = 9/15 (60%) Query: 111 AKYPDYEVTWANDGY 125 AKYPDY W GY Sbjct: 419 AKYPDYFPEWKKSGY 433 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2OZX-A nrPDB 262 3e-71 2HW4-A nrPDB 257 8e-70 Sequences not found previously or not previously below threshold: 3F7O-A nrPDB 30 0.26 5UCY-B nrPDB 29 0.57 6GJ4-B nrPDB 28 0.84 6E88-B nrPDB 28 0.91 3WBE-A nrPDB 28 1.0 5IJ0-B nrPDB 28 1.2 6DEO-A nrPDB 28 1.4 5GSL-A nrPDB 27 1.9 4R27-A nrPDB 27 2.1 3N29-A nrPDB 27 2.6 2J7A-A nrPDB 27 3.1 3L4Y-A nrPDB 26 3.5 5A3V-A nrPDB 26 3.8 4I6M-B nrPDB 26 5.6 3WEE-A nrPDB 26 5.6 CONVERGED! >2OZX-A nrPDB Length = 125 Score = 262 bits (670), Expect = 3e-71, Method: Composition-based stats. Identities = 125/125 (100%), Positives = 125/125 (100%) Query: 1 MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKV 60 MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKV Sbjct: 1 MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKV 60 Query: 61 SGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTW 120 SGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTW Sbjct: 61 SGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTW 120 Query: 121 ANDGY 125 ANDGY Sbjct: 121 ANDGY 125 >2HW4-A nrPDB Length = 144 Score = 257 bits (657), Expect = 8e-70, Method: Composition-based stats. Identities = 121/121 (100%), Positives = 121/121 (100%) Query: 5 DLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDM 64 DLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDM Sbjct: 24 DLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDM 83 Query: 65 QKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDG 124 QKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDG Sbjct: 84 QKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDG 143 Query: 125 Y 125 Y Sbjct: 144 Y 144 >3F7O-A nrPDB Length = 284 Score = 30.0 bits (66), Expect = 0.26, Method: Composition-based stats. Identities = 15/43 (34%), Positives = 24/43 (55%), Gaps = 1/43 (2%) Query: 75 GGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYE 117 G GRISH+S+ + Y S G + I T ++A +P++E Sbjct: 11 GLGRISHRSKGSTTYEYDTSGGSGTCAYVIDT-GVEASHPEFE 52 >5UCY-B nrPDB Length = 429 Score = 28.9 bits (63), Expect = 0.57, Method: Composition-based stats. Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 4/40 (10%) Query: 46 GYKWAEYH----ADIYDKVSGDMQKQGCDCECLGGGRISH 81 G WA+ H A++ D V ++K+ C+CL G +I+H Sbjct: 98 GNNWAKGHYTEGAELIDSVLDVVRKEAEGCDCLQGFQITH 137 >6GJ4-B nrPDB Length = 445 Score = 28.5 bits (62), Expect = 0.84, Method: Composition-based stats. Identities = 19/74 (25%), Positives = 32/74 (43%), Gaps = 14/74 (18%) Query: 46 GYKWAEYH----ADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQ 101 G WA+ H A++ D V ++K+ C+CL G +++H G + Sbjct: 98 GNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLL------ 151 Query: 102 HAISTEKIKAKYPD 115 KI+ +YPD Sbjct: 152 ----ISKIREEYPD 161 >6E88-B nrPDB Length = 426 Score = 28.1 bits (61), Expect = 0.91, Method: Composition-based stats. Identities = 19/74 (25%), Positives = 32/74 (43%), Gaps = 14/74 (18%) Query: 46 GYKWAEYH----ADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQ 101 G WA+ H A++ D V ++K+ C+CL G +++H G + Sbjct: 98 GNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLL------ 151 Query: 102 HAISTEKIKAKYPD 115 KI+ +YPD Sbjct: 152 ----ISKIREEYPD 161 >3WBE-A nrPDB Length = 489 Score = 28.1 bits (61), Expect = 1.0, Method: Composition-based stats. Identities = 25/111 (22%), Positives = 42/111 (37%), Gaps = 15/111 (13%) Query: 19 VFKYVLIRVHSAP---------RSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGC 69 + YV R +S P S P K+ ++ K +YH D ++ +++ GC Sbjct: 378 LMNYVKERYNSPPVYITENGMDDSNNPFISIKDALKDSKRIKYHNDYLTNLAASIKEDGC 437 Query: 70 DCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTW 120 D G + D GYS +G + + + +YP V W Sbjct: 438 DVR----GYFAWSLLDNWEWAAGYSSRFG--LYFVDYKDNLKRYPKNSVQW 482 >5IJ0-B nrPDB Length = 426 Score = 27.7 bits (60), Expect = 1.2, Method: Composition-based stats. Identities = 13/40 (32%), Positives = 24/40 (60%), Gaps = 4/40 (10%) Query: 46 GYKWAEYH----ADIYDKVSGDMQKQGCDCECLGGGRISH 81 G WA+ H A++ D V ++K+ +C+CL G +++H Sbjct: 98 GNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTH 137 >6DEO-A nrPDB Length = 682 Score = 27.7 bits (60), Expect = 1.4, Method: Composition-based stats. Identities = 13/30 (43%), Positives = 17/30 (56%), Gaps = 3/30 (10%) Query: 10 PD---VDIDSDGVFKYVLIRVHSAPRSGAP 36 PD +DID D F L + SA ++GAP Sbjct: 535 PDAIVIDIDGDASFNMTLTELSSAVQAGAP 564 >5GSL-A nrPDB Length = 778 Score = 27.3 bits (59), Expect = 1.9, Method: Composition-based stats. Identities = 20/85 (23%), Positives = 38/85 (44%), Gaps = 16/85 (18%) Query: 5 DLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDM 64 ++ LIP + +D++G+ + V+ + + R K E A I DKV G + Sbjct: 569 NMRLIPYLSVDAEGIDRMVV----------------RNVAREVKGGEAIAWIGDKVVGTI 612 Query: 65 QKQGCDCECLGGGRISHQSQDKKIH 89 ++G + G R+ + S +H Sbjct: 613 VRKGKGSAVILGFRLQYYSSYHDMH 637 >4R27-A nrPDB Length = 434 Score = 26.9 bits (58), Expect = 2.1, Method: Composition-based stats. Identities = 11/21 (52%), Positives = 12/21 (57%) Query: 100 AQHAISTEKIKAKYPDYEVTW 120 A H +TE IKA PD V W Sbjct: 217 AAHKAATEAIKAHRPDLRVGW 237 >3N29-A nrPDB Length = 418 Score = 26.6 bits (57), Expect = 2.6, Method: Composition-based stats. Identities = 12/33 (36%), Positives = 18/33 (54%), Gaps = 1/33 (3%) Query: 59 KVSGDMQKQGCDCECLGGGRISHQSQDKKIHVY 91 K+ G+ K GC C L + + + DK+IH Y Sbjct: 86 KIVGEYLK-GCTCSGLWEAKFAKEYMDKEIHTY 117 >2J7A-A nrPDB Length = 500 Score = 26.6 bits (57), Expect = 3.1, Method: Composition-based stats. Identities = 14/36 (38%), Positives = 20/36 (55%), Gaps = 2/36 (5%) Query: 88 IHVYGYSMAYGPAQHAIS-TEKIKAKYPDYEVTWAN 122 + V G+ + H S T IKA++P+YE TW N Sbjct: 248 LQVKGFEGKFADWTHPASKTPMIKAQHPEYE-TWIN 282 >3L4Y-A nrPDB Length = 875 Score = 26.2 bits (56), Expect = 3.5, Method: Composition-based stats. Identities = 17/55 (30%), Positives = 25/55 (45%), Gaps = 1/55 (1%) Query: 71 CECLGGGRISHQSQDKKIH-VYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDG 124 C+ L + H + IH +YGYSMA A+ A + K + T+A G Sbjct: 479 CKTLCMDAVQHWGKQYDIHNLYGYSMAVATAEAAKTVFPNKRSFILTRSTFAGSG 533 >5A3V-A nrPDB Length = 329 Score = 26.2 bits (56), Expect = 3.8, Method: Composition-based stats. Identities = 13/41 (31%), Positives = 23/41 (56%) Query: 74 LGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYP 114 +G G + ++ DK + V + G A+ A++TEK+ K P Sbjct: 80 VGSGVKNFKAGDKVVAVLSHLGGGGLAEFAVATEKLTVKRP 120 >4I6M-B nrPDB Length = 439 Score = 25.8 bits (55), Expect = 5.6, Method: Composition-based stats. Identities = 9/15 (60%), Positives = 9/15 (60%) Query: 111 AKYPDYEVTWANDGY 125 AKYPDY W GY Sbjct: 382 AKYPDYFPEWKKSGY 396 >3WEE-A nrPDB Length = 476 Score = 25.8 bits (55), Expect = 5.6, Method: Composition-based stats. Identities = 9/15 (60%), Positives = 9/15 (60%) Query: 111 AKYPDYEVTWANDGY 125 AKYPDY W GY Sbjct: 419 AKYPDYFPEWKKSGY 433 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.317 0.134 0.416 Lambda K H 0.267 0.0411 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,901,302 Number of Sequences: 36641 Number of extensions: 233656 Number of successful extensions: 458 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 23 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 435 Number of HSP's gapped (non-prelim): 35 length of query: 125 length of database: 10,336,785 effective HSP length: 85 effective length of query: 40 effective length of database: 7,222,300 effective search space: 288892000 effective search space used: 288892000 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 53 (25.0 bits)