BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9NRJ3 (127 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6CWS-A nrPDB 224 1e-59 2KUM-A nrPDB 65 6e-12 1HA6-A nrPDB 32 0.058 2JYO-A nrPDB 28 1.1 3ODW-A nrPDB 27 2.6 2PQF-A nrPDB 26 5.3 6GB2-BC nrPDB 25 6.1 3P6A-A nrPDB 25 8.7 1ZXT-A nrPDB 25 9.2 >6CWS-A nrPDB Length = 108 Score = 224 bits (570), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 108/108 (100%), Positives = 108/108 (100%) Query: 20 SEAILPIASSCCTEVSHHISRRLLERVNMCRIQRADGDCDLAAVILHVKRRRICVSPHNH 79 SEAILPIASSCCTEVSHHISRRLLERVNMCRIQRADGDCDLAAVILHVKRRRICVSPHNH Sbjct: 1 SEAILPIASSCCTEVSHHISRRLLERVNMCRIQRADGDCDLAAVILHVKRRRICVSPHNH 60 Query: 80 TVKQWMKVQAAKKNGKGNVCHRKKHHGKRNSNRAHQGKHETYGHKTPY 127 TVKQWMKVQAAKKNGKGNVCHRKKHHGKRNSNRAHQGKHETYGHKTPY Sbjct: 61 TVKQWMKVQAAKKNGKGNVCHRKKHHGKRNSNRAHQGKHETYGHKTPY 108 >2KUM-A nrPDB Length = 88 Score = 65.5 bits (158), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 29/73 (39%), Positives = 47/73 (64%), Gaps = 1/73 (1%) Query: 23 ILPIASSCCTEVSHH-ISRRLLERVNMCRIQRADGDCDLAAVILHVKRRRICVSPHNHTV 81 +LP +++CCT++ +S +LL +V +Q ADGDC L A +LH+ +R IC+ P N ++ Sbjct: 2 LLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSICIHPQNPSL 61 Query: 82 KQWMKVQAAKKNG 94 QW + Q K +G Sbjct: 62 SQWFEHQERKLHG 74 >1HA6-A nrPDB Length = 70 Score = 32.3 bits (72), Expect = 0.058, Method: Compositional matrix adjust. Identities = 14/33 (42%), Positives = 21/33 (63%), Gaps = 1/33 (3%) Query: 52 QRADGDCDLAAVILHVKRRR-ICVSPHNHTVKQ 83 Q AD CD+ A+I H K+R+ +C P + VK+ Sbjct: 26 QMADEACDINAIIFHTKKRKSVCADPKQNWVKR 58 >2JYO-A nrPDB Length = 70 Score = 28.1 bits (61), Expect = 1.1, Method: Compositional matrix adjust. Identities = 13/41 (31%), Positives = 24/41 (58%), Gaps = 1/41 (2%) Query: 52 QRADGDCDLAAVILHVKRR-RICVSPHNHTVKQWMKVQAAK 91 Q A+ CD+ A+I H K++ +C +P VK +++ + K Sbjct: 26 QLANEGCDINAIIFHTKKKLSVCANPKQTWVKYIVRLLSKK 66 >3ODW-A nrPDB Length = 536 Score = 26.9 bits (58), Expect = 2.6, Method: Composition-based stats. Identities = 9/36 (25%), Positives = 22/36 (61%) Query: 26 IASSCCTEVSHHISRRLLERVNMCRIQRADGDCDLA 61 +A+ CC E+ HH+++ + + ++ R++ DL+ Sbjct: 354 LAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLS 389 >2PQF-A nrPDB Length = 198 Score = 25.8 bits (55), Expect = 5.3, Method: Compositional matrix adjust. Identities = 12/26 (46%), Positives = 14/26 (53%) Query: 81 VKQWMKVQAAKKNGKGNVCHRKKHHG 106 V QW K Q K+NG V R+ HG Sbjct: 54 VYQWQKGQMQKQNGGKAVDERQLFHG 79 >6GB2-BC nrPDB Length = 657 Score = 25.4 bits (54), Expect = 6.1, Method: Composition-based stats. Identities = 14/51 (27%), Positives = 22/51 (43%), Gaps = 8/51 (15%) Query: 76 PHNHTVKQWMKVQAAKKNGKGN---VCHRKKHHGKRNSNRAHQGKHETYGH 123 P V W K + ++ G+ + + ++K H AHQ E YGH Sbjct: 368 PRAREVVDWRKYEQEQEKGQEDLKIIEEKRKEH-----KEAHQKAREKYGH 413 >3P6A-A nrPDB Length = 377 Score = 25.0 bits (53), Expect = 8.7, Method: Compositional matrix adjust. Identities = 9/36 (25%), Positives = 22/36 (61%) Query: 26 IASSCCTEVSHHISRRLLERVNMCRIQRADGDCDLA 61 +A+ CC E+ HH+++ + + ++ R++ DL+ Sbjct: 201 LAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLS 236 >1ZXT-A nrPDB Length = 76 Score = 25.0 bits (53), Expect = 9.2, Method: Compositional matrix adjust. Identities = 13/30 (43%), Positives = 17/30 (56%), Gaps = 1/30 (3%) Query: 58 CDLAAVILHVKR-RRICVSPHNHTVKQWMK 86 C VIL KR R+IC P + V+Q M+ Sbjct: 35 CPKPGVILLTKRGRQICADPSKNWVRQLMQ 64 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6CWS-A nrPDB 172 3e-44 2KUM-A nrPDB 121 6e-29 Sequences not found previously or not previously below threshold: 2RA4-A nrPDB 29 0.53 1HA6-A nrPDB 29 0.56 2JYO-A nrPDB 27 3.2 6GB2-BC nrPDB 26 4.5 5BV1-B nrPDB 26 5.0 5BV0-B nrPDB 26 5.0 1K9V-F nrPDB 26 5.7 6I0M-A nrPDB 25 6.7 4DQ6-A nrPDB 25 8.1 5OOL-Y nrPDB 25 9.4 CONVERGED! >6CWS-A nrPDB Length = 108 Score = 172 bits (436), Expect = 3e-44, Method: Composition-based stats. Identities = 108/108 (100%), Positives = 108/108 (100%) Query: 20 SEAILPIASSCCTEVSHHISRRLLERVNMCRIQRADGDCDLAAVILHVKRRRICVSPHNH 79 SEAILPIASSCCTEVSHHISRRLLERVNMCRIQRADGDCDLAAVILHVKRRRICVSPHNH Sbjct: 1 SEAILPIASSCCTEVSHHISRRLLERVNMCRIQRADGDCDLAAVILHVKRRRICVSPHNH 60 Query: 80 TVKQWMKVQAAKKNGKGNVCHRKKHHGKRNSNRAHQGKHETYGHKTPY 127 TVKQWMKVQAAKKNGKGNVCHRKKHHGKRNSNRAHQGKHETYGHKTPY Sbjct: 61 TVKQWMKVQAAKKNGKGNVCHRKKHHGKRNSNRAHQGKHETYGHKTPY 108 >2KUM-A nrPDB Length = 88 Score = 121 bits (304), Expect = 6e-29, Method: Composition-based stats. Identities = 29/73 (39%), Positives = 47/73 (64%), Gaps = 1/73 (1%) Query: 23 ILPIASSCCTEVSHH-ISRRLLERVNMCRIQRADGDCDLAAVILHVKRRRICVSPHNHTV 81 +LP +++CCT++ +S +LL +V +Q ADGDC L A +LH+ +R IC+ P N ++ Sbjct: 2 LLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSICIHPQNPSL 61 Query: 82 KQWMKVQAAKKNG 94 QW + Q K +G Sbjct: 62 SQWFEHQERKLHG 74 >2RA4-A nrPDB Length = 76 Score = 28.9 bits (63), Expect = 0.53, Method: Composition-based stats. Identities = 19/74 (25%), Positives = 31/74 (41%), Gaps = 6/74 (8%) Query: 21 EAILPIASSCCTEVSHHISRRLLERVNMCRIQRADGDCDLAAVILHVK-RRRICVSPHNH 79 +A+ ++ C T S IS + L+ + + C AVI K + IC P Sbjct: 4 DALNVPSTCCFTFSSKKISLQRLKSYVITTSR-----CPQKAVIFRTKLGKEICADPKEK 58 Query: 80 TVKQWMKVQAAKKN 93 V+ +MK K + Sbjct: 59 WVQNYMKHLGRKAH 72 >1HA6-A nrPDB Length = 70 Score = 28.9 bits (63), Expect = 0.56, Method: Composition-based stats. Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 1/33 (3%) Query: 52 QRADGDCDLAAVILHVKRR-RICVSPHNHTVKQ 83 Q AD CD+ A+I H K+R +C P + VK+ Sbjct: 26 QMADEACDINAIIFHTKKRKSVCADPKQNWVKR 58 >2JYO-A nrPDB Length = 70 Score = 26.6 bits (57), Expect = 3.2, Method: Composition-based stats. Identities = 13/45 (28%), Positives = 23/45 (51%), Gaps = 5/45 (11%) Query: 52 QRADGDCDLAAVILHVKRR-RICVSPHNHTVKQWMKVQAAKKNGK 95 Q A+ CD+ A+I H K++ +C +P + W+K + K Sbjct: 26 QLANEGCDINAIIFHTKKKLSVCANPK----QTWVKYIVRLLSKK 66 >6GB2-BC nrPDB Length = 657 Score = 25.8 bits (55), Expect = 4.5, Method: Composition-based stats. Identities = 14/51 (27%), Positives = 22/51 (43%), Gaps = 8/51 (15%) Query: 76 PHNHTVKQWMKVQAAKKNGKGN---VCHRKKHHGKRNSNRAHQGKHETYGH 123 P V W K + ++ G+ + + ++K H AHQ E YGH Sbjct: 368 PRAREVVDWRKYEQEQEKGQEDLKIIEEKRKEH-----KEAHQKAREKYGH 413 >5BV1-B nrPDB Length = 333 Score = 25.8 bits (55), Expect = 5.0, Method: Composition-based stats. Identities = 12/48 (25%), Positives = 21/48 (43%), Gaps = 6/48 (12%) Query: 56 GDCDLAAVILHVKRRRICVS------PHNHTVKQWMKVQAAKKNGKGN 97 GD DL ++H RR++ ++ T ++ A +G GN Sbjct: 55 GDTDLIYFVIHQLRRKLPLASFFRVVSSRPTASAMVEALARNSDGDGN 102 >5BV0-B nrPDB Length = 229 Score = 25.8 bits (55), Expect = 5.0, Method: Composition-based stats. Identities = 12/48 (25%), Positives = 21/48 (43%), Gaps = 6/48 (12%) Query: 56 GDCDLAAVILHVKRRRICVS------PHNHTVKQWMKVQAAKKNGKGN 97 GD DL ++H RR++ ++ T ++ A +G GN Sbjct: 55 GDTDLIYFVIHQLRRKLPLASFFRVVSSRPTASAMVEALARNSDGDGN 102 >1K9V-F nrPDB Length = 201 Score = 25.8 bits (55), Expect = 5.7, Method: Composition-based stats. Identities = 10/21 (47%), Positives = 13/21 (61%) Query: 33 EVSHHISRRLLERVNMCRIQR 53 E S I R+LLE+V C + R Sbjct: 180 EKSSKIGRKLLEKVIECSLSR 200 >6I0M-A nrPDB Length = 517 Score = 25.4 bits (54), Expect = 6.7, Method: Composition-based stats. Identities = 12/45 (26%), Positives = 23/45 (51%), Gaps = 2/45 (4%) Query: 51 IQRADGDCDLAAVILHVKRRRICVSPHNHTVKQWMKVQAAKKNGK 95 ++ + DC L ++ KR + V+P T+K+ ++ K GK Sbjct: 169 LKEEEHDCFLEEIM--TKREDLVVAPAGITLKEANEILQRSKKGK 211 >4DQ6-A nrPDB Length = 391 Score = 25.0 bits (53), Expect = 8.1, Method: Composition-based stats. Identities = 14/43 (32%), Positives = 21/43 (48%), Gaps = 5/43 (11%) Query: 72 ICVSPHNHTVKQWMKVQAAKKNGKGNVCHRKKHHGKRNSNRAH 114 I +PHN + W K + K G++C KH+ K S+ H Sbjct: 169 ILCNPHNPVGRVWTKDELKKL---GDIC--LKHNVKIISDEIH 206 >5OOL-Y nrPDB Length = 250 Score = 25.0 bits (53), Expect = 9.4, Method: Composition-based stats. Identities = 10/32 (31%), Positives = 17/32 (53%) Query: 1 MQQRGLAIVALAVCAALHASEAILPIASSCCT 32 M GLA++ V +AL +S +++ CT Sbjct: 1 MAAAGLALLCRRVSSALKSSRSLITPQVPACT 32 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.319 0.133 0.410 Lambda K H 0.267 0.0409 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,639,608 Number of Sequences: 36641 Number of extensions: 201529 Number of successful extensions: 488 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 475 Number of HSP's gapped (non-prelim): 26 length of query: 127 length of database: 10,336,785 effective HSP length: 86 effective length of query: 41 effective length of database: 7,185,659 effective search space: 294612019 effective search space used: 294612019 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.3 bits) S2: 53 (25.0 bits)