BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9NPF7 (189 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5MJ3-B nrPDB 345 6e-96 2L3Y-A nrPDB 30 0.66 2IBU-A nrPDB 28 2.0 4HA6-A nrPDB 27 4.5 4JLC-A nrPDB 27 5.2 1CD9-A nrPDB 27 5.9 4B4H-A nrPDB 27 6.9 1BGD-A nrPDB 27 7.4 2H31-A nrPDB 26 8.0 3UBL-A nrPDB 26 9.5 >5MJ3-B nrPDB Length = 179 Score = 345 bits (886), Expect = 6e-96, Method: Compositional matrix adjust. Identities = 170/170 (100%), Positives = 170/170 (100%) Query: 20 RAVPGGSSPAWTQCQQLSQKLCTLAWSAHPLVGHMDLREEGDEETTNDVPHIQCGDGCDP 79 RAVPGGSSPAWTQCQQLSQKLCTLAWSAHPLVGHMDLREEGDEETTNDVPHIQCGDGCDP Sbjct: 1 RAVPGGSSPAWTQCQQLSQKLCTLAWSAHPLVGHMDLREEGDEETTNDVPHIQCGDGCDP 60 Query: 80 QGLRDNSQFCLQRIHQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEG 139 QGLRDNSQFCLQRIHQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEG Sbjct: 61 QGLRDNSQFCLQRIHQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEG 120 Query: 140 HHWETQQIPSLSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAATLSP 189 HHWETQQIPSLSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAATLSP Sbjct: 121 HHWETQQIPSLSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAATLSP 170 >2L3Y-A nrPDB Length = 190 Score = 30.0 bits (66), Expect = 0.66, Method: Compositional matrix adjust. Identities = 19/55 (34%), Positives = 25/55 (45%), Gaps = 4/55 (7%) Query: 52 GHMDLREEGDEETTNDV--PHIQCGDGCDPQGLRDNSQFCLQRIHQGLIFYEKLL 104 G+ D D N++ P IQ DGC G N + CL +I GL+ Y L Sbjct: 51 GNSDCMNNDDALAENNLKLPEIQRNDGCYQTGY--NQEICLLKISSGLLEYHSYL 103 >2IBU-A nrPDB Length = 395 Score = 28.5 bits (62), Expect = 2.0, Method: Compositional matrix adjust. Identities = 23/91 (25%), Positives = 38/91 (41%), Gaps = 16/91 (17%) Query: 20 RAVPGGSSPAWTQCQQLSQKLCT------LAWSAHPLVGHMDLREEGDEETTNDVPHIQC 73 +AV G P T C ++ K+C + S + GH D+ G E+ ++VP++ Sbjct: 74 QAVLGAGLPISTPCTTIN-KVCASGMKAIMMASQSLMCGHQDVMVAGGMESMSNVPYVM- 131 Query: 74 GDGCDPQG--------LRDNSQFCLQRIHQG 96 G P G ++D +IH G Sbjct: 132 NRGSTPYGGVKLEDLIVKDGLTDVYNKIHMG 162 >4HA6-A nrPDB Length = 508 Score = 27.3 bits (59), Expect = 4.5, Method: Compositional matrix adjust. Identities = 9/36 (25%), Positives = 22/36 (61%) Query: 106 SDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEGHH 141 ++F + S++P+ G +HA++L +++ + HH Sbjct: 471 DNLFVVDASIMPNLTAGPIHAAVLAIAETFARQYHH 506 >4JLC-A nrPDB Length = 657 Score = 26.9 bits (58), Expect = 5.2, Method: Composition-based stats. Identities = 17/61 (27%), Positives = 28/61 (45%), Gaps = 9/61 (14%) Query: 88 FCLQRIHQGLIFYEKLLGSDIFTGEPSLLPD---------SPVGQLHASLLGLSQLLQPE 138 FC++ I + + YEKL+ ++ E + D S G + +SL +S L P Sbjct: 471 FCIRNIEKTVKVYEKLMKVNLEAAELGEISDIHTKLLRLSSSQGTIESSLQDISSRLSPG 530 Query: 139 G 139 G Sbjct: 531 G 531 >1CD9-A nrPDB Length = 175 Score = 26.9 bits (58), Expect = 5.9, Method: Compositional matrix adjust. Identities = 30/104 (28%), Positives = 44/104 (42%), Gaps = 13/104 (12%) Query: 89 CLQRIHQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEGHHWETQQIP 148 CL ++H GL Y+ LL + + P L P QL + + Q E E P Sbjct: 75 CLSQLHSGLFLYQGLLQA-LEGISPELGPTLDTLQLDVADFATTIWQQME----ELGMAP 129 Query: 149 SLSPSQ--------PWQRLLLRFKILRSLQAFVAVAARVFAHGA 184 +L P+Q +QR + LQ+F+ V+ RV H A Sbjct: 130 ALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLA 173 >4B4H-A nrPDB Length = 420 Score = 26.6 bits (57), Expect = 6.9, Method: Compositional matrix adjust. Identities = 13/44 (29%), Positives = 21/44 (47%) Query: 15 WTAQGRAVPGGSSPAWTQCQQLSQKLCTLAWSAHPLVGHMDLRE 58 W+A+ A PGGS+ A ++ + + P G M LR+ Sbjct: 18 WSAKAAAEPGGSAVANESTAVWLDRIGAIEGNDSPTTGSMGLRD 61 >1BGD-A nrPDB Length = 175 Score = 26.6 bits (57), Expect = 7.4, Method: Compositional matrix adjust. Identities = 27/104 (25%), Positives = 46/104 (44%), Gaps = 13/104 (12%) Query: 89 CLQRIHQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEGHHWETQQIP 148 CL+++H GL Y+ LL + + P L P QL + ++ Q E + P Sbjct: 75 CLRQLHSGLFLYQGLLQA-LAGISPELAPTLDTLQLDTTDFAINIWQQME----DLGMAP 129 Query: 149 SLSPSQ--------PWQRLLLRFKILRSLQAFVAVAARVFAHGA 184 ++ P+Q +QR + +LQ+F+ +A R H A Sbjct: 130 AVPPTQGTMPAFTSAFQRRAGGVLVASNLQSFLELAYRALRHFA 173 >2H31-A nrPDB Length = 425 Score = 26.2 bits (56), Expect = 8.0, Method: Compositional matrix adjust. Identities = 23/61 (37%), Positives = 28/61 (45%), Gaps = 7/61 (11%) Query: 113 PSLLPDSPVGQLHASL-----LGLSQLLQPEGHHWETQQIPSLSPSQPWQRLLLRFKILR 167 P L PD V + +SL LG S +L PEG QI LS W + LR IL Sbjct: 351 PPLTPDWGVQDVWSSLRLPSGLGCSTVLSPEGSAQFAAQIFGLSNHLVWSK--LRASILN 408 Query: 168 S 168 + Sbjct: 409 T 409 >3UBL-A nrPDB Length = 242 Score = 26.2 bits (56), Expect = 9.5, Method: Compositional matrix adjust. Identities = 14/44 (31%), Positives = 23/44 (52%), Gaps = 1/44 (2%) Query: 146 QIPSLSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAATLSP 189 Q P L P PW+ +R +I ++ ++ + AR AA +SP Sbjct: 77 QTPKLIPEDPWEAARVR-EISTIIETYLDIPARRIYLPAAKVSP 119 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5MJ3-B nrPDB 351 1e-97 Sequences not found previously or not previously below threshold: 2L3Y-A nrPDB 30 0.44 1CD9-A nrPDB 30 0.76 2H31-A nrPDB 29 1.1 1BGD-A nrPDB 29 1.5 6B9X-E nrPDB 29 1.5 4HA6-A nrPDB 28 2.4 4B4H-A nrPDB 28 3.5 3UBL-A nrPDB 28 3.5 5I8U-G nrPDB 27 5.3 4JLC-A nrPDB 27 6.0 2IBU-A nrPDB 27 6.4 CONVERGED! >5MJ3-B nrPDB Length = 179 Score = 351 bits (901), Expect = 1e-97, Method: Composition-based stats. Identities = 170/170 (100%), Positives = 170/170 (100%) Query: 20 RAVPGGSSPAWTQCQQLSQKLCTLAWSAHPLVGHMDLREEGDEETTNDVPHIQCGDGCDP 79 RAVPGGSSPAWTQCQQLSQKLCTLAWSAHPLVGHMDLREEGDEETTNDVPHIQCGDGCDP Sbjct: 1 RAVPGGSSPAWTQCQQLSQKLCTLAWSAHPLVGHMDLREEGDEETTNDVPHIQCGDGCDP 60 Query: 80 QGLRDNSQFCLQRIHQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEG 139 QGLRDNSQFCLQRIHQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEG Sbjct: 61 QGLRDNSQFCLQRIHQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEG 120 Query: 140 HHWETQQIPSLSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAATLSP 189 HHWETQQIPSLSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAATLSP Sbjct: 121 HHWETQQIPSLSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAATLSP 170 >2L3Y-A nrPDB Length = 190 Score = 30.4 bits (67), Expect = 0.44, Method: Composition-based stats. Identities = 19/55 (34%), Positives = 25/55 (45%), Gaps = 4/55 (7%) Query: 52 GHMDLREEGDEETTNDV--PHIQCGDGCDPQGLRDNSQFCLQRIHQGLIFYEKLL 104 G+ D D N++ P IQ DGC G N + CL +I GL+ Y L Sbjct: 51 GNSDCMNNDDALAENNLKLPEIQRNDGCYQTGY--NQEICLLKISSGLLEYHSYL 103 >1CD9-A nrPDB Length = 175 Score = 29.6 bits (65), Expect = 0.76, Method: Composition-based stats. Identities = 30/104 (28%), Positives = 44/104 (42%), Gaps = 13/104 (12%) Query: 89 CLQRIHQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEGHHWETQQIP 148 CL ++H GL Y+ LL + + P L P QL + + Q E E P Sbjct: 75 CLSQLHSGLFLYQGLLQA-LEGISPELGPTLDTLQLDVADFATTIWQQME----ELGMAP 129 Query: 149 SLSPSQ--------PWQRLLLRFKILRSLQAFVAVAARVFAHGA 184 +L P+Q +QR + LQ+F+ V+ RV H A Sbjct: 130 ALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLA 173 >2H31-A nrPDB Length = 425 Score = 29.2 bits (64), Expect = 1.1, Method: Composition-based stats. Identities = 20/52 (38%), Positives = 24/52 (46%), Gaps = 5/52 (9%) Query: 113 PSLLPDSPVGQLHASL-----LGLSQLLQPEGHHWETQQIPSLSPSQPWQRL 159 P L PD V + +SL LG S +L PEG QI LS W +L Sbjct: 351 PPLTPDWGVQDVWSSLRLPSGLGCSTVLSPEGSAQFAAQIFGLSNHLVWSKL 402 >1BGD-A nrPDB Length = 175 Score = 28.9 bits (63), Expect = 1.5, Method: Composition-based stats. Identities = 27/104 (25%), Positives = 46/104 (44%), Gaps = 13/104 (12%) Query: 89 CLQRIHQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEGHHWETQQIP 148 CL+++H GL Y+ LL + + P L P QL + ++ Q E + P Sbjct: 75 CLRQLHSGLFLYQGLLQA-LAGISPELAPTLDTLQLDTTDFAINIWQQME----DLGMAP 129 Query: 149 SLSPSQ--------PWQRLLLRFKILRSLQAFVAVAARVFAHGA 184 ++ P+Q +QR + +LQ+F+ +A R H A Sbjct: 130 AVPPTQGTMPAFTSAFQRRAGGVLVASNLQSFLELAYRALRHFA 173 >6B9X-E nrPDB Length = 173 Score = 28.9 bits (63), Expect = 1.5, Method: Composition-based stats. Identities = 25/82 (30%), Positives = 30/82 (36%), Gaps = 19/82 (23%) Query: 17 AQGRAVPGGSSPAWTQCQQLSQKLCTLAWSAHPLVGHMDLREEGDEETTNDVPHIQCGDG 76 +GRAVP GS P W+ VG M+ E E T P I Sbjct: 65 GEGRAVPLGSEPEWS-------------------VGGMEATLEQHLEDTMKNPSIVGVLC 105 Query: 77 CDPQGLRDNSQFCLQRIHQGLI 98 D QGL + L H G+I Sbjct: 106 TDSQGLNLGCRGTLSDEHAGVI 127 >4HA6-A nrPDB Length = 508 Score = 28.1 bits (61), Expect = 2.4, Method: Composition-based stats. Identities = 9/35 (25%), Positives = 22/35 (62%) Query: 107 DIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEGHH 141 ++F + S++P+ G +HA++L +++ + HH Sbjct: 472 NLFVVDASIMPNLTAGPIHAAVLAIAETFARQYHH 506 >4B4H-A nrPDB Length = 420 Score = 27.7 bits (60), Expect = 3.5, Method: Composition-based stats. Identities = 14/48 (29%), Positives = 23/48 (47%) Query: 15 WTAQGRAVPGGSSPAWTQCQQLSQKLCTLAWSAHPLVGHMDLREEGDE 62 W+A+ A PGGS+ A ++ + + P G M LR+ +E Sbjct: 18 WSAKAAAEPGGSAVANESTAVWLDRIGAIEGNDSPTTGSMGLRDHLEE 65 >3UBL-A nrPDB Length = 242 Score = 27.7 bits (60), Expect = 3.5, Method: Composition-based stats. Identities = 14/44 (31%), Positives = 23/44 (52%), Gaps = 1/44 (2%) Query: 146 QIPSLSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAATLSP 189 Q P L P PW+ +R +I ++ ++ + AR AA +SP Sbjct: 77 QTPKLIPEDPWEAARVR-EISTIIETYLDIPARRIYLPAAKVSP 119 >5I8U-G nrPDB Length = 207 Score = 26.9 bits (58), Expect = 5.3, Method: Composition-based stats. Identities = 16/57 (28%), Positives = 26/57 (45%), Gaps = 4/57 (7%) Query: 130 GLSQLLQPEGHHWETQQIPSLSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAAT 186 GL ++ +PE HH + P R +LR +I+ S +A+A + H T Sbjct: 129 GLREVGRPEAHHEQADMTMGWYPIAEAARRVLRGEIVNS----IAIAGVLAVHAVTT 181 >4JLC-A nrPDB Length = 657 Score = 26.9 bits (58), Expect = 6.0, Method: Composition-based stats. Identities = 17/61 (27%), Positives = 28/61 (45%), Gaps = 9/61 (14%) Query: 88 FCLQRIHQGLIFYEKLLGSDIFTGEPSLLPD---------SPVGQLHASLLGLSQLLQPE 138 FC++ I + + YEKL+ ++ E + D S G + +SL +S L P Sbjct: 471 FCIRNIEKTVKVYEKLMKVNLEAAELGEISDIHTKLLRLSSSQGTIESSLQDISSRLSPG 530 Query: 139 G 139 G Sbjct: 531 G 531 >2IBU-A nrPDB Length = 395 Score = 26.5 bits (57), Expect = 6.4, Method: Composition-based stats. Identities = 23/91 (25%), Positives = 38/91 (41%), Gaps = 16/91 (17%) Query: 20 RAVPGGSSPAWTQCQQLSQKLCT------LAWSAHPLVGHMDLREEGDEETTNDVPHIQC 73 +AV G P T C ++ K+C + S + GH D+ G E+ ++VP++ Sbjct: 74 QAVLGAGLPISTPCTTIN-KVCASGMKAIMMASQSLMCGHQDVMVAGGMESMSNVPYVM- 131 Query: 74 GDGCDPQG--------LRDNSQFCLQRIHQG 96 G P G ++D +IH G Sbjct: 132 NRGSTPYGGVKLEDLIVKDGLTDVYNKIHMG 162 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.321 0.136 0.434 Lambda K H 0.267 0.0420 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,523,683 Number of Sequences: 36641 Number of extensions: 372030 Number of successful extensions: 656 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 20 Number of HSP's that attempted gapping in prelim test: 650 Number of HSP's gapped (non-prelim): 26 length of query: 189 length of database: 10,336,785 effective HSP length: 91 effective length of query: 98 effective length of database: 7,002,454 effective search space: 686240492 effective search space used: 686240492 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 56 (26.1 bits)