BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9LY46 (487 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6CCI-A nrPDB 1014 0.0 4IJB-A nrPDB 31 1.3 1EV1-3 nrPDB 29 4.6 3EJE-A nrPDB 28 9.6 >6CCI-A nrPDB Length = 487 Score = 1014 bits (2622), Expect = 0.0, Method: Compositional matrix adjust. Identities = 487/487 (100%), Positives = 487/487 (100%) Query: 1 MQPWRRKFPLFETGVTMKQRKNSNLSIFVVVFSVFLFGIFMYNEDVKSIAEFPFSTSKPH 60 MQPWRRKFPLFETGVTMKQRKNSNLSIFVVVFSVFLFGIFMYNEDVKSIAEFPFSTSKPH Sbjct: 1 MQPWRRKFPLFETGVTMKQRKNSNLSIFVVVFSVFLFGIFMYNEDVKSIAEFPFSTSKPH 60 Query: 61 DVHDEATPITEITTLPVQESIKNSDPIQESIKNADSVQDSVKDVAEPVQEEVSKTEEVKK 120 DVHDEATPITEITTLPVQESIKNSDPIQESIKNADSVQDSVKDVAEPVQEEVSKTEEVKK Sbjct: 61 DVHDEATPITEITTLPVQESIKNSDPIQESIKNADSVQDSVKDVAEPVQEEVSKTEEVKK 120 Query: 121 IELFAATEDEEDVELPPEECDLFTGEWVFDNETHPLYKEDQCEFLTAQVTCMRNGRRDSL 180 IELFAATEDEEDVELPPEECDLFTGEWVFDNETHPLYKEDQCEFLTAQVTCMRNGRRDSL Sbjct: 121 IELFAATEDEEDVELPPEECDLFTGEWVFDNETHPLYKEDQCEFLTAQVTCMRNGRRDSL 180 Query: 181 YQNWRWQPRDCSLPKFKAKLLLEKLRNKRMMFVGDSLNRNQWESMVCLVQSVVPPGRKSL 240 YQNWRWQPRDCSLPKFKAKLLLEKLRNKRMMFVGDSLNRNQWESMVCLVQSVVPPGRKSL Sbjct: 181 YQNWRWQPRDCSLPKFKAKLLLEKLRNKRMMFVGDSLNRNQWESMVCLVQSVVPPGRKSL 240 Query: 241 NKTGSLSVFRVEDYNATVEFYWAPFLVESNSDDPNMHSILNRIIMPESIEKHGVNWKGVD 300 NKTGSLSVFRVEDYNATVEFYWAPFLVESNSDDPNMHSILNRIIMPESIEKHGVNWKGVD Sbjct: 241 NKTGSLSVFRVEDYNATVEFYWAPFLVESNSDDPNMHSILNRIIMPESIEKHGVNWKGVD 300 Query: 301 FLVFNTYIWWMNTFAMKVLRGSFDKGDTEYEEIERPVAYRRVMRTWGDWVERNIDPLRTT 360 FLVFNTYIWWMNTFAMKVLRGSFDKGDTEYEEIERPVAYRRVMRTWGDWVERNIDPLRTT Sbjct: 301 FLVFNTYIWWMNTFAMKVLRGSFDKGDTEYEEIERPVAYRRVMRTWGDWVERNIDPLRTT 360 Query: 361 VFFASMSPLHIKSLDWENPDGIKCALETTPILNMSMPFSVGTDYRLFSVAENVTHSLNVP 420 VFFASMSPLHIKSLDWENPDGIKCALETTPILNMSMPFSVGTDYRLFSVAENVTHSLNVP Sbjct: 361 VFFASMSPLHIKSLDWENPDGIKCALETTPILNMSMPFSVGTDYRLFSVAENVTHSLNVP 420 Query: 421 VYFLNITKLSEYRKDAHTSVHTIRQGKMLTPEQQADPNTYADCIHWCLPGLPDTWNEFLY 480 VYFLNITKLSEYRKDAHTSVHTIRQGKMLTPEQQADPNTYADCIHWCLPGLPDTWNEFLY Sbjct: 421 VYFLNITKLSEYRKDAHTSVHTIRQGKMLTPEQQADPNTYADCIHWCLPGLPDTWNEFLY 480 Query: 481 TRIISRS 487 TRIISRS Sbjct: 481 TRIISRS 487 >4IJB-A nrPDB Length = 256 Score = 30.8 bits (68), Expect = 1.3, Method: Compositional matrix adjust. Identities = 21/74 (28%), Positives = 34/74 (45%) Query: 93 NADSVQDSVKDVAEPVQEEVSKTEEVKKIELFAATEDEEDVELPPEECDLFTGEWVFDNE 152 AD+V VK + E E + + +E DE D+++ F G W DNE Sbjct: 126 GADTVALKVKILTERELESLLEYARSYGMEPLIEINDENDLDIALRIGARFIGIWSQDNE 185 Query: 153 THPLYKEDQCEFLT 166 T + KE+Q + ++ Sbjct: 186 TLEINKENQRKLIS 199 >1EV1-3 nrPDB Length = 239 Score = 29.3 bits (64), Expect = 4.6, Method: Compositional matrix adjust. Identities = 22/79 (27%), Positives = 35/79 (44%), Gaps = 11/79 (13%) Query: 229 VQSVVPPGRKSLNKTGSLSVFRVE---DYNATVEFYWAPFLVESNSDDPNMHSILNRIIM 285 V SV+P S K S+ +RVE + NA + + +P S++NR +M Sbjct: 49 VDSVMPINNDSAAKVSSMEAYRVELSTNTNAGTQVFGFQL-------NPGAESVMNRTLM 101 Query: 286 PESIEKHGVNWKGVDFLVF 304 E I + +W G + F Sbjct: 102 GE-ILNYYAHWSGSIKITF 119 >3EJE-A nrPDB Length = 97 Score = 28.1 bits (61), Expect = 9.6, Method: Composition-based stats. Identities = 21/67 (31%), Positives = 30/67 (44%), Gaps = 15/67 (22%) Query: 87 IQESIKNADSVQDSVKDVAEPVQEEVSKT---------EEVKKIELFAATEDEEDVELPP 137 I+E +K Q VK QEEV+ + + +EL A E+E D E+P Sbjct: 23 IEERVKKIIGEQLGVK------QEEVTNNASFVEDLGADSLDTVELVMALEEEFDTEIPD 76 Query: 138 EECDLFT 144 EE + T Sbjct: 77 EEAEKIT 83 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6CCI-A nrPDB 1070 0.0 Sequences not found previously or not previously below threshold: 3JA6-G nrPDB 32 0.76 2BGQ-A nrPDB 32 0.85 4IJB-A nrPDB 32 0.88 5A6D-A nrPDB 31 1.1 3G71-G nrPDB 31 1.1 5OHN-A nrPDB 30 2.9 6HIV-Cd nrPDB 29 5.0 4IHH-G nrPDB 28 6.5 3EJE-A nrPDB 28 6.5 3S27-A nrPDB 28 7.0 CONVERGED! >6CCI-A nrPDB Length = 487 Score = 1070 bits (2767), Expect = 0.0, Method: Composition-based stats. Identities = 487/487 (100%), Positives = 487/487 (100%) Query: 1 MQPWRRKFPLFETGVTMKQRKNSNLSIFVVVFSVFLFGIFMYNEDVKSIAEFPFSTSKPH 60 MQPWRRKFPLFETGVTMKQRKNSNLSIFVVVFSVFLFGIFMYNEDVKSIAEFPFSTSKPH Sbjct: 1 MQPWRRKFPLFETGVTMKQRKNSNLSIFVVVFSVFLFGIFMYNEDVKSIAEFPFSTSKPH 60 Query: 61 DVHDEATPITEITTLPVQESIKNSDPIQESIKNADSVQDSVKDVAEPVQEEVSKTEEVKK 120 DVHDEATPITEITTLPVQESIKNSDPIQESIKNADSVQDSVKDVAEPVQEEVSKTEEVKK Sbjct: 61 DVHDEATPITEITTLPVQESIKNSDPIQESIKNADSVQDSVKDVAEPVQEEVSKTEEVKK 120 Query: 121 IELFAATEDEEDVELPPEECDLFTGEWVFDNETHPLYKEDQCEFLTAQVTCMRNGRRDSL 180 IELFAATEDEEDVELPPEECDLFTGEWVFDNETHPLYKEDQCEFLTAQVTCMRNGRRDSL Sbjct: 121 IELFAATEDEEDVELPPEECDLFTGEWVFDNETHPLYKEDQCEFLTAQVTCMRNGRRDSL 180 Query: 181 YQNWRWQPRDCSLPKFKAKLLLEKLRNKRMMFVGDSLNRNQWESMVCLVQSVVPPGRKSL 240 YQNWRWQPRDCSLPKFKAKLLLEKLRNKRMMFVGDSLNRNQWESMVCLVQSVVPPGRKSL Sbjct: 181 YQNWRWQPRDCSLPKFKAKLLLEKLRNKRMMFVGDSLNRNQWESMVCLVQSVVPPGRKSL 240 Query: 241 NKTGSLSVFRVEDYNATVEFYWAPFLVESNSDDPNMHSILNRIIMPESIEKHGVNWKGVD 300 NKTGSLSVFRVEDYNATVEFYWAPFLVESNSDDPNMHSILNRIIMPESIEKHGVNWKGVD Sbjct: 241 NKTGSLSVFRVEDYNATVEFYWAPFLVESNSDDPNMHSILNRIIMPESIEKHGVNWKGVD 300 Query: 301 FLVFNTYIWWMNTFAMKVLRGSFDKGDTEYEEIERPVAYRRVMRTWGDWVERNIDPLRTT 360 FLVFNTYIWWMNTFAMKVLRGSFDKGDTEYEEIERPVAYRRVMRTWGDWVERNIDPLRTT Sbjct: 301 FLVFNTYIWWMNTFAMKVLRGSFDKGDTEYEEIERPVAYRRVMRTWGDWVERNIDPLRTT 360 Query: 361 VFFASMSPLHIKSLDWENPDGIKCALETTPILNMSMPFSVGTDYRLFSVAENVTHSLNVP 420 VFFASMSPLHIKSLDWENPDGIKCALETTPILNMSMPFSVGTDYRLFSVAENVTHSLNVP Sbjct: 361 VFFASMSPLHIKSLDWENPDGIKCALETTPILNMSMPFSVGTDYRLFSVAENVTHSLNVP 420 Query: 421 VYFLNITKLSEYRKDAHTSVHTIRQGKMLTPEQQADPNTYADCIHWCLPGLPDTWNEFLY 480 VYFLNITKLSEYRKDAHTSVHTIRQGKMLTPEQQADPNTYADCIHWCLPGLPDTWNEFLY Sbjct: 421 VYFLNITKLSEYRKDAHTSVHTIRQGKMLTPEQQADPNTYADCIHWCLPGLPDTWNEFLY 480 Query: 481 TRIISRS 487 TRIISRS Sbjct: 481 TRIISRS 487 >3JA6-G nrPDB Length = 309 Score = 31.6 bits (70), Expect = 0.76, Method: Composition-based stats. Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 11/57 (19%) Query: 62 VHDEATPITEITTLPVQESIKNSDPIQESIKNADSVQDSVKDVAEPVQEEVSKTEEV 118 + ++ + EITT + E+ KNA+ + +SVK+V +QE + TEEV Sbjct: 242 IEEQTAAVDEITT-----------AMTENAKNAEEITNSVKEVNARLQEISASTEEV 287 >2BGQ-A nrPDB Length = 344 Score = 31.6 bits (70), Expect = 0.85, Method: Composition-based stats. Identities = 31/115 (26%), Positives = 50/115 (43%), Gaps = 15/115 (13%) Query: 54 FSTSKPHDVHDEATPITEITTLPVQESIKNSDPIQESIKNADSVQDSVKDVAEPVQE--- 110 F K H +H IT + L E DP+ E + N + + +Q Sbjct: 225 FEACKKHGIH-----ITAYSPLGSSEKNLAHDPVVEKVANKLNKTPGQVLIKWALQRGTS 279 Query: 111 ---EVSKTEEVKK-IELFAATEDEEDVEL---PPEECDLFTGEWVFDNETHPLYK 158 + SK E +K+ I++F EED ++ +E + TGE +F N+TH Y+ Sbjct: 280 VIPKSSKDERIKENIQVFGWEIPEEDFKVLCSIKDEKRVLTGEELFVNKTHGPYR 334 >4IJB-A nrPDB Length = 256 Score = 31.6 bits (70), Expect = 0.88, Method: Composition-based stats. Identities = 21/73 (28%), Positives = 34/73 (46%) Query: 94 ADSVQDSVKDVAEPVQEEVSKTEEVKKIELFAATEDEEDVELPPEECDLFTGEWVFDNET 153 AD+V VK + E E + + +E DE D+++ F G W DNET Sbjct: 127 ADTVALKVKILTERELESLLEYARSYGMEPLIEINDENDLDIALRIGARFIGIWSQDNET 186 Query: 154 HPLYKEDQCEFLT 166 + KE+Q + ++ Sbjct: 187 LEINKENQRKLIS 199 >5A6D-A nrPDB Length = 249 Score = 31.2 bits (69), Expect = 1.1, Method: Composition-based stats. Identities = 17/53 (32%), Positives = 31/53 (58%) Query: 87 IQESIKNADSVQDSVKDVAEPVQEEVSKTEEVKKIELFAATEDEEDVELPPEE 139 ++E+IK+A V DS+K +A+ + + E ++E+ EDE ++L EE Sbjct: 141 LKEAIKDASLVSDSLKFIAKEDEFTMKAEGETNEVEIKLTLEDEGLLDLEVEE 193 >3G71-G nrPDB Length = 348 Score = 31.2 bits (69), Expect = 1.1, Method: Composition-based stats. Identities = 23/98 (23%), Positives = 47/98 (47%), Gaps = 10/98 (10%) Query: 42 YNEDVKSIAEFPFSTSKPHDVHDEATPITEITT-------LPVQESIKNSDPIQESIKNA 94 Y D+++ A F+ S D T T + + L +Q +I++ + + + + A Sbjct: 215 YRSDIQAAAGRAFNLSVNADYPTATTAPTMLQSARGNAKSLALQAAIEDPEVVPDLVSKA 274 Query: 95 DSVQDSVKDVAEPVQEEVSKTEEVKKIELFAATEDEED 132 D+ V+ +A + +E + EE++ +E ATE+ D Sbjct: 275 DA---QVRALASQIDDEEALPEELQGVEADVATEEPTD 309 >5OHN-A nrPDB Length = 370 Score = 29.6 bits (65), Expect = 2.9, Method: Composition-based stats. Identities = 29/99 (29%), Positives = 41/99 (41%), Gaps = 18/99 (18%) Query: 95 DSVQDS--VKDVAEPVQEEVSKTEEVKKIELF----AATEDEEDVELPPEECDLFTGEWV 148 D V D+ + DV + ++S EE ELF ++ EDE D + P LF Sbjct: 74 DEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITSSLEDERDRQ--PRVTHLF----- 126 Query: 149 FDNETHPLYKEDQCEFLTAQVTCMRNGRRDSLYQNWRWQ 187 + H L E Q E Q+TC G +W+ Q Sbjct: 127 ---DVHSL--EQQSEITPKQITCRTRGSPHPTSNHWKSQ 160 >6HIV-Cd nrPDB Length = 440 Score = 28.9 bits (63), Expect = 5.0, Method: Composition-based stats. Identities = 21/83 (25%), Positives = 44/83 (53%), Gaps = 4/83 (4%) Query: 49 IAEFPFSTSKPHDVHDEATPITEITTLPVQESIKNSDPIQESIKNADSVQDSVKDVAEPV 108 +AE P ST+KPH+ H ++ I+E + +E +K + + D + ++E + Sbjct: 205 MAEHPDSTAKPHEPHTSSS-ISEAAAFEERMLRAMVSAKEEDLK--EWLGDDSRALSETI 261 Query: 109 QEEVSKTEEVKKIELFAATEDEE 131 +++S+ EE ++ ++ A EE Sbjct: 262 -DDISREEEEREADIRVARSMEE 283 >4IHH-G nrPDB Length = 80 Score = 28.5 bits (62), Expect = 6.5, Method: Composition-based stats. Identities = 21/67 (31%), Positives = 30/67 (44%), Gaps = 15/67 (22%) Query: 87 IQESIKNADSVQDSVKDVAEPVQEEVSKT---------EEVKKIELFAATEDEEDVELPP 137 I+E +K Q VK QEEV+ + + +EL A E+E D E+P Sbjct: 6 IEERVKKIIGEQLGVK------QEEVTNNASFVEDLGADSLDTVELVMALEEEFDTEIPD 59 Query: 138 EECDLFT 144 EE + T Sbjct: 60 EEAEKIT 66 >3EJE-A nrPDB Length = 97 Score = 28.5 bits (62), Expect = 6.5, Method: Composition-based stats. Identities = 21/67 (31%), Positives = 30/67 (44%), Gaps = 15/67 (22%) Query: 87 IQESIKNADSVQDSVKDVAEPVQEEVSKT---------EEVKKIELFAATEDEEDVELPP 137 I+E +K Q VK QEEV+ + + +EL A E+E D E+P Sbjct: 23 IEERVKKIIGEQLGVK------QEEVTNNASFVEDLGADSLDTVELVMALEEEFDTEIPD 76 Query: 138 EECDLFT 144 EE + T Sbjct: 77 EEAEKIT 83 >3S27-A nrPDB Length = 816 Score = 28.5 bits (62), Expect = 7.0, Method: Composition-based stats. Identities = 14/34 (41%), Positives = 18/34 (52%) Query: 325 KGDTEYEEIERPVAYRRVMRTWGDWVERNIDPLR 358 K +T YEE E + R WGD ER +D +R Sbjct: 221 KSETLYEEFEAKFEEIGLERGWGDNAERVLDMIR 254 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.319 0.134 0.417 Lambda K H 0.267 0.0413 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 20,463,578 Number of Sequences: 36641 Number of extensions: 860697 Number of successful extensions: 2111 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 20 Number of HSP's that attempted gapping in prelim test: 2085 Number of HSP's gapped (non-prelim): 40 length of query: 487 length of database: 10,336,785 effective HSP length: 101 effective length of query: 386 effective length of database: 6,636,044 effective search space: 2561512984 effective search space used: 2561512984 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 61 (28.1 bits)