BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= Q9LSX0
         (131 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

5WX9-A nrPDB                                                          266   1e-72
3GCC-A nrPDB                                                           99   8e-22
5EV3-A nrPDB                                                           29   0.72 
1JD7-A nrPDB                                                           27   3.0  
5KCG-A nrPDB                                                           27   3.7  
6CA8-A nrPDB                                                           26   5.9  
2QA2-A nrPDB                                                           25   8.5  

>5WX9-A nrPDB
          Length = 131

 Score =  266 bits (681), Expect = 1e-72,   Method: Compositional matrix adjust.
 Identities = 131/131 (100%), Positives = 131/131 (100%)

Query: 1   MDQGGRGVGAEHGKYRGVRRRPWGKYAAEIRDSRKHGERVWLGTFDTAEEAARAYDQAAY 60
           MDQGGRGVGAEHGKYRGVRRRPWGKYAAEIRDSRKHGERVWLGTFDTAEEAARAYDQAAY
Sbjct: 1   MDQGGRGVGAEHGKYRGVRRRPWGKYAAEIRDSRKHGERVWLGTFDTAEEAARAYDQAAY 60

Query: 61  SMRGQAAILNFPHEYNMGSGVSSSTAMAGSSSASASASSSSRQVFEFEYLDDSVLEELLE 120
           SMRGQAAILNFPHEYNMGSGVSSSTAMAGSSSASASASSSSRQVFEFEYLDDSVLEELLE
Sbjct: 61  SMRGQAAILNFPHEYNMGSGVSSSTAMAGSSSASASASSSSRQVFEFEYLDDSVLEELLE 120

Query: 121 EGEKPNKGKKK 131
           EGEKPNKGKKK
Sbjct: 121 EGEKPNKGKKK 131


>3GCC-A nrPDB
          Length = 70

 Score = 98.6 bits (244), Expect = 8e-22,   Method: Compositional matrix adjust.
 Identities = 45/64 (70%), Positives = 53/64 (82%)

Query: 15 YRGVRRRPWGKYAAEIRDSRKHGERVWLGTFDTAEEAARAYDQAAYSMRGQAAILNFPHE 74
          YRGVR+RPWGK+AAEIRD  K+G RVWLGTF+TAE+AA AYD+AA+ MRG  A+LNFP  
Sbjct: 6  YRGVRQRPWGKFAAEIRDPAKNGARVWLGTFETAEDAALAYDRAAFRMRGSRALLNFPLR 65

Query: 75 YNMG 78
           N G
Sbjct: 66 VNSG 69


>5EV3-A nrPDB
          Length = 201

 Score = 28.9 bits (63), Expect = 0.72,   Method: Compositional matrix adjust.
 Identities = 19/78 (24%), Positives = 36/78 (46%), Gaps = 3/78 (3%)

Query: 45  FDTAEEAARAYDQAAYSMRGQAAILNFPHEYNMGSGVSSSTAMAGSSSASASASSSSRQV 104
           F + +E  +A        +GQ+  +  PH+Y    G+S + ++      S     S+ ++
Sbjct: 62  FRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKL 121

Query: 105 F---EFEYLDDSVLEELL 119
           F      YL+D  ++ELL
Sbjct: 122 FIGGLPNYLNDDQVKELL 139


>1JD7-A nrPDB
          Length = 453

 Score = 26.6 bits (57), Expect = 3.0,   Method: Composition-based stats.
 Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 2/45 (4%)

Query: 1   MDQGGRGVGAEHGKYRGVRRRPWGKYAAEIRDSRKHGERVWLGTF 45
           +DQGG  VGA      G+      KY+ E+ ++ ++G   WL  F
Sbjct: 202 IDQGGEAVGASEYLSTGLVTE--FKYSTELGNTFRNGSLAWLSNF 244


>5KCG-A nrPDB
          Length = 419

 Score = 26.6 bits (57), Expect = 3.7,   Method: Composition-based stats.
 Identities = 18/59 (30%), Positives = 25/59 (42%), Gaps = 3/59 (5%)

Query: 21 RPWGKYAAEIRDSRKHGERVWLGTFDTAEEAARAYDQAAYSMRGQAAILNFPHEYNMGS 79
          RPW   +  +       E  W    +TAE   R  +Q  YS+  Q   L F H+Y + S
Sbjct: 6  RPWQILSQALGFPNYDQELWWQ---NTAETLNRVLEQCDYSVHLQYKYLAFYHKYILPS 61


>6CA8-A nrPDB
          Length = 751

 Score = 25.8 bits (55), Expect = 5.9,   Method: Composition-based stats.
 Identities = 18/42 (42%), Positives = 25/42 (59%), Gaps = 8/42 (19%)

Query: 89  GSSSASASA------SSSSRQVFEFEYLDDSVLEELLEEGEK 124
           G   ASA+       +SS+R +F  EY DD +L+ L EEG+K
Sbjct: 353 GGKDASAARYIFTKLASSTRSIFN-EY-DDPILKYLNEEGQK 392


>2QA2-A nrPDB
          Length = 499

 Score = 25.0 bits (53), Expect = 8.5,   Method: Compositional matrix adjust.
 Identities = 11/37 (29%), Positives = 17/37 (45%)

Query: 36  HGERVWLGTFDTAEEAARAYDQAAYSMRGQAAILNFP 72
           HGE VW+  F        AY +    + G +A ++ P
Sbjct: 256 HGEPVWVSAFGDPARQVSAYRRGRVLLAGDSAHVHLP 292


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

5WX9-A nrPDB                                                          227   1e-60
3GCC-A nrPDB                                                          119   3e-28

Sequences not found previously or not previously below threshold:

1JD7-A nrPDB                                                           31   0.15 
6CA8-A nrPDB                                                           28   1.2  
6DLF-A nrPDB                                                           28   1.6  
2CV2-A nrPDB                                                           27   2.3  
3I64-A nrPDB                                                           27   2.5  
5TSD-A nrPDB                                                           26   4.0  
4DIO-A nrPDB                                                           26   4.1  
2QA2-A nrPDB                                                           26   5.2  


CONVERGED!
>5WX9-A nrPDB
          Length = 131

 Score =  227 bits (578), Expect = 1e-60,   Method: Composition-based stats.
 Identities = 131/131 (100%), Positives = 131/131 (100%)

Query: 1   MDQGGRGVGAEHGKYRGVRRRPWGKYAAEIRDSRKHGERVWLGTFDTAEEAARAYDQAAY 60
           MDQGGRGVGAEHGKYRGVRRRPWGKYAAEIRDSRKHGERVWLGTFDTAEEAARAYDQAAY
Sbjct: 1   MDQGGRGVGAEHGKYRGVRRRPWGKYAAEIRDSRKHGERVWLGTFDTAEEAARAYDQAAY 60

Query: 61  SMRGQAAILNFPHEYNMGSGVSSSTAMAGSSSASASASSSSRQVFEFEYLDDSVLEELLE 120
           SMRGQAAILNFPHEYNMGSGVSSSTAMAGSSSASASASSSSRQVFEFEYLDDSVLEELLE
Sbjct: 61  SMRGQAAILNFPHEYNMGSGVSSSTAMAGSSSASASASSSSRQVFEFEYLDDSVLEELLE 120

Query: 121 EGEKPNKGKKK 131
           EGEKPNKGKKK
Sbjct: 121 EGEKPNKGKKK 131


>3GCC-A nrPDB
          Length = 70

 Score =  119 bits (299), Expect = 3e-28,   Method: Composition-based stats.
 Identities = 46/69 (66%), Positives = 55/69 (79%)

Query: 10 AEHGKYRGVRRRPWGKYAAEIRDSRKHGERVWLGTFDTAEEAARAYDQAAYSMRGQAAIL 69
          A+   YRGVR+RPWGK+AAEIRD  K+G RVWLGTF+TAE+AA AYD+AA+ MRG  A+L
Sbjct: 1  AKGKHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTFETAEDAALAYDRAAFRMRGSRALL 60

Query: 70 NFPHEYNMG 78
          NFP   N G
Sbjct: 61 NFPLRVNSG 69


>1JD7-A nrPDB
          Length = 453

 Score = 30.9 bits (68), Expect = 0.15,   Method: Composition-based stats.
 Identities = 15/45 (33%), Positives = 23/45 (51%), Gaps = 2/45 (4%)

Query: 1   MDQGGRGVGAEHGKYRGVRRRPWGKYAAEIRDSRKHGERVWLGTF 45
           +DQGG  VGA      G+      KY+ E+ ++ ++G   WL  F
Sbjct: 202 IDQGGEAVGASEYLSTGLVTE--FKYSTELGNTFRNGSLAWLSNF 244


>6CA8-A nrPDB
          Length = 751

 Score = 28.2 bits (61), Expect = 1.2,   Method: Composition-based stats.
 Identities = 18/42 (42%), Positives = 25/42 (59%), Gaps = 8/42 (19%)

Query: 89  GSSSASASA------SSSSRQVFEFEYLDDSVLEELLEEGEK 124
           G   ASA+       +SS+R +F  EY DD +L+ L EEG+K
Sbjct: 353 GGKDASAARYIFTKLASSTRSIFN-EY-DDPILKYLNEEGQK 392


>6DLF-A nrPDB
          Length = 306

 Score = 27.8 bits (60), Expect = 1.6,   Method: Composition-based stats.
 Identities = 20/75 (26%), Positives = 33/75 (44%), Gaps = 7/75 (9%)

Query: 50  EAARAYDQAAYSMRGQAAILNFPHEYNMGSGVSSSTAMAGSSSASASASSSSRQVFEFEY 109
           E + + D AA  +R     +N+P   +   G        G+    ASA  S+    EF++
Sbjct: 178 ECSASNDVAAPVVRRVKVTVNYPPYISEAKGTGVPVGQKGTLQCEASAVPSA----EFQW 233

Query: 110 LDDSVLEELLEEGEK 124
             D   ++ L EG+K
Sbjct: 234 YKD---DKRLIEGKK 245


>2CV2-A nrPDB
          Length = 468

 Score = 27.0 bits (58), Expect = 2.3,   Method: Composition-based stats.
 Identities = 19/48 (39%), Positives = 23/48 (47%), Gaps = 8/48 (16%)

Query: 5   GRGVGAEHGKYRGVRRRP-WGKYAAEIRDSRKHGERVW-LGTFDTAEE 50
           G  VG  HG YR   R P + KYA E+       +R W    F+T EE
Sbjct: 70  GPDVGGPHGPYRQSERLPLYQKYAEELL------KRGWAYRAFETPEE 111


>3I64-A nrPDB
          Length = 332

 Score = 27.0 bits (58), Expect = 2.5,   Method: Composition-based stats.
 Identities = 21/86 (24%), Positives = 34/86 (39%), Gaps = 8/86 (9%)

Query: 25  KYAAEIRDSRKHGERVWLGTFDTAEEAARAYDQAAYSMR-GQAAILNFPHEYNMGSGVSS 83
           ++  ++RD    G+R WL            + + A+S+R GQ A   +P  Y    G S 
Sbjct: 80  EFGEQLRDDHAAGKRKWLDMNSAVGRGDLGFVELAHSIRTGQPA---YPVRY----GTSF 132

Query: 84  STAMAGSSSASASASSSSRQVFEFEY 109
              +      SAS  +      E +Y
Sbjct: 133 WEDLGSDPVLSASFDTLMSHHLELDY 158


>5TSD-A nrPDB
          Length = 322

 Score = 26.2 bits (56), Expect = 4.0,   Method: Composition-based stats.
 Identities = 22/80 (27%), Positives = 29/80 (36%), Gaps = 1/80 (1%)

Query: 40  VWLGTFDTAEEAARAYDQAAYSMRGQAAILNFPHEYNMGSGVSSSTAMAGSSSASASASS 99
           + +G      E    YD   +    +   L  P   N G G S       S+    +   
Sbjct: 198 ILVGLLPLTPETTGFYDSELFKKLRRDGALGQPVFINAGRGKSQIETDIVSAVREGTLGG 257

Query: 100 SSRQVFEFEYL-DDSVLEEL 118
           +S  VFE E L  DS L EL
Sbjct: 258 ASLDVFEVEPLATDSPLWEL 277


>4DIO-A nrPDB
          Length = 405

 Score = 26.2 bits (56), Expect = 4.1,   Method: Composition-based stats.
 Identities = 16/59 (27%), Positives = 27/59 (45%)

Query: 42  LGTFDTAEEAARAYDQAAYSMRGQAAILNFPHEYNMGSGVSSSTAMAGSSSASASASSS 100
           L  +    +AA  YD+A   M   A  +     + MG+GV+   A+A +    A  S++
Sbjct: 161 LAGYQAVIDAAYEYDRALPMMMTAAGTVPAAKIFVMGAGVAGLQAIATARRLGAVVSAT 219


>2QA2-A nrPDB
          Length = 499

 Score = 25.9 bits (55), Expect = 5.2,   Method: Composition-based stats.
 Identities = 11/37 (29%), Positives = 17/37 (45%)

Query: 36  HGERVWLGTFDTAEEAARAYDQAAYSMRGQAAILNFP 72
           HGE VW+  F        AY +    + G +A ++ P
Sbjct: 256 HGEPVWVSAFGDPARQVSAYRRGRVLLAGDSAHVHLP 292


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.307    0.128    0.363 

Lambda     K      H
   0.267   0.0394    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,367,778
Number of Sequences: 36641
Number of extensions: 193696
Number of successful extensions: 635
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 418
Number of HSP's gapped (non-prelim): 82
length of query: 131
length of database: 10,336,785
effective HSP length: 86
effective length of query: 45
effective length of database: 7,185,659
effective search space: 323354655
effective search space used: 323354655
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.5 bits)
S2: 53 (25.1 bits)