BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9LNF2 (176 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1X90-A nrPDB 303 3e-83 1XG2-B nrPDB 102 1e-22 3ZOK-A nrPDB 32 0.11 2BNX-A nrPDB 29 1.0 6AXE-A nrPDB 26 7.6 >1X90-A nrPDB Length = 152 Score = 303 bits (776), Expect = 3e-83, Method: Compositional matrix adjust. Identities = 148/149 (99%), Positives = 148/149 (99%) Query: 28 SSEMSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDSTQARATQTLKKLQSIIDGGV 87 SSEMSTICDKTLNPSFCLKFLNTKFAS NLQALAKTTLDSTQARATQTLKKLQSIIDGGV Sbjct: 4 SSEMSTICDKTLNPSFCLKFLNTKFASANLQALAKTTLDSTQARATQTLKKLQSIIDGGV 63 Query: 88 DPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRS 147 DPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRS Sbjct: 64 DPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRS 123 Query: 148 VDSSVVNNSKTIKNLCGIALVISNMLPRN 176 VDSSVVNNSKTIKNLCGIALVISNMLPRN Sbjct: 124 VDSSVVNNSKTIKNLCGIALVISNMLPRN 152 >1XG2-B nrPDB Length = 153 Score = 102 bits (253), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 57/146 (39%), Positives = 86/146 (58%), Gaps = 2/146 (1%) Query: 31 MSTICDKTLNPSFCLKFLNT--KFASPNLQALAKTTLDSTQARATQTLKKLQSIIDGGVD 88 +S IC KT NPS CL+ L + + AS +L+ L + ++D QA A QT K + S+ + D Sbjct: 6 ISEICPKTRNPSLCLQALESDPRSASKDLKGLGQFSIDIAQASAKQTSKIIASLTNQATD 65 Query: 89 PRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRSV 148 P+ K Y +C + Y AI +L +A + L SGD +N+ SAA DGA TC D + ++ Sbjct: 66 PKLKGRYETCSENYADAIDSLGQAKQFLTSGDYNSLNIYASAAFDGAGTCEDSFEGPPNI 125 Query: 149 DSSVVNNSKTIKNLCGIALVISNMLP 174 + + +++LC I LVISN+LP Sbjct: 126 PTQLHQADLKLEDLCDIVLVISNLLP 151 >3ZOK-A nrPDB Length = 378 Score = 32.3 bits (72), Expect = 0.11, Method: Compositional matrix adjust. Identities = 30/87 (34%), Positives = 39/87 (44%), Gaps = 16/87 (18%) Query: 88 DPRSKLAY---RSC--------VDEYESAIG---NLEEAFEH-LASGDGMGMNMKVSAAL 132 DP S LAY RSC +DE ES + NL F H + +G G G + A Sbjct: 226 DP-SALAYAIKRSCENKAEVVSLDEKESGLRATLNLGHTFGHAIETGFGYGQWLHGEAVA 284 Query: 133 DGADTCLDDVKRLRSVDSSVVNNSKTI 159 G +D RL +D S+VN + I Sbjct: 285 AGMVMAVDMSYRLGWIDESIVNRAHNI 311 >2BNX-A nrPDB Length = 386 Score = 29.3 bits (64), Expect = 1.0, Method: Compositional matrix adjust. Identities = 23/81 (28%), Positives = 35/81 (43%), Gaps = 9/81 (11%) Query: 10 FLSSLMFLLLIGSSYAITSSEMSTI--CDKTL-------NPSFCLKFLNTKFASPNLQAL 60 FLS L LLL+ + Y I C + +P F + L Q + Sbjct: 271 FLSILQHLLLVRNDYEARPQYYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIERLVDQMI 330 Query: 61 AKTTLDSTQARATQTLKKLQS 81 KT ++ ++A+AT+ KKL S Sbjct: 331 DKTKVEKSEAKATELEKKLDS 351 >6AXE-A nrPDB Length = 739 Score = 26.2 bits (56), Expect = 7.6, Method: Composition-based stats. Identities = 13/44 (29%), Positives = 21/44 (47%) Query: 10 FLSSLMFLLLIGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFA 53 FL+ + +LL + IT+S + T P + LN +FA Sbjct: 92 FLTEIGYLLPAPEDFTITTSGVDDEITTTAGPQLVVPILNARFA 135 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1X90-A nrPDB 239 6e-64 1XG2-B nrPDB 219 5e-58 Sequences not found previously or not previously below threshold: 1RJ1-A nrPDB 47 3e-06 6BPQ-A nrPDB 30 0.40 6AXE-A nrPDB 29 1.6 6APZ-A nrPDB 27 3.5 4BD9-B nrPDB 27 3.9 6AYO-A nrPDB 27 5.0 5BQN-A nrPDB 27 5.6 5BQM-B nrPDB 26 7.2 >1X90-A nrPDB Length = 152 Score = 239 bits (609), Expect = 6e-64, Method: Composition-based stats. Identities = 148/149 (99%), Positives = 148/149 (99%) Query: 28 SSEMSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDSTQARATQTLKKLQSIIDGGV 87 SSEMSTICDKTLNPSFCLKFLNTKFAS NLQALAKTTLDSTQARATQTLKKLQSIIDGGV Sbjct: 4 SSEMSTICDKTLNPSFCLKFLNTKFASANLQALAKTTLDSTQARATQTLKKLQSIIDGGV 63 Query: 88 DPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRS 147 DPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRS Sbjct: 64 DPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRS 123 Query: 148 VDSSVVNNSKTIKNLCGIALVISNMLPRN 176 VDSSVVNNSKTIKNLCGIALVISNMLPRN Sbjct: 124 VDSSVVNNSKTIKNLCGIALVISNMLPRN 152 >1XG2-B nrPDB Length = 153 Score = 219 bits (558), Expect = 5e-58, Method: Composition-based stats. Identities = 57/146 (39%), Positives = 86/146 (58%), Gaps = 2/146 (1%) Query: 31 MSTICDKTLNPSFCLKFLNT--KFASPNLQALAKTTLDSTQARATQTLKKLQSIIDGGVD 88 +S IC KT NPS CL+ L + + AS +L+ L + ++D QA A QT K + S+ + D Sbjct: 6 ISEICPKTRNPSLCLQALESDPRSASKDLKGLGQFSIDIAQASAKQTSKIIASLTNQATD 65 Query: 89 PRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDDVKRLRSV 148 P+ K Y +C + Y AI +L +A + L SGD +N+ SAA DGA TC D + ++ Sbjct: 66 PKLKGRYETCSENYADAIDSLGQAKQFLTSGDYNSLNIYASAAFDGAGTCEDSFEGPPNI 125 Query: 149 DSSVVNNSKTIKNLCGIALVISNMLP 174 + + +++LC I LVISN+LP Sbjct: 126 PTQLHQADLKLEDLCDIVLVISNLLP 151 >1RJ1-A nrPDB Length = 151 Score = 47.4 bits (111), Expect = 3e-06, Method: Composition-based stats. Identities = 31/141 (21%), Positives = 60/141 (42%), Gaps = 3/141 (2%) Query: 25 AITSSEMSTICDKTLNPSFCLKFL--NTKFASPNLQALAKTTLDSTQARATQTLKKLQSI 82 A+ ++ + T C T N CLK L + + A+ ++ LA +D+ +A+A Q + + Sbjct: 2 AMGNNLVETTCKNTPNYQLCLKTLLSDKRSATGDITTLALIMVDAIKAKANQAAVTISKL 61 Query: 83 IDGGVDPRSKLAYRSCVDEYESAI-GNLEEAFEHLASGDGMGMNMKVSAALDGADTCLDD 141 K ++C Y+ + +L EA E L GD + + A C + Sbjct: 62 RHSNPPAAWKGPLKNCAFSYKVILTASLPEAIEALTKGDPKFAEDGMVGSSGDAQECEEY 121 Query: 142 VKRLRSVDSSVVNNSKTIKNL 162 K +S S++ + ++ Sbjct: 122 FKGSKSPFSALNIAVHELSDV 142 >6BPQ-A nrPDB Length = 950 Score = 30.5 bits (67), Expect = 0.40, Method: Composition-based stats. Identities = 19/78 (24%), Positives = 31/78 (39%), Gaps = 6/78 (7%) Query: 30 EMSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDSTQA---RATQTLKKLQSIIDGG 86 E+S C T +P L+ L N + L+K + T+ A K L+S+ Sbjct: 424 ELSEECPITRHP---LQALFIWSVLQNKKELSKVIWEQTRGCTLAALGASKLLKSMAKVK 480 Query: 87 VDPRSKLAYRSCVDEYES 104 D + +EYE+ Sbjct: 481 NDINAAGESEELANEYET 498 >6AXE-A nrPDB Length = 739 Score = 28.5 bits (62), Expect = 1.6, Method: Composition-based stats. Identities = 13/44 (29%), Positives = 21/44 (47%) Query: 10 FLSSLMFLLLIGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFA 53 FL+ + +LL + IT+S + T P + LN +FA Sbjct: 92 FLTEIGYLLPAPEDFTITTSGVDDEITTTAGPQLVVPILNARFA 135 >6APZ-A nrPDB Length = 741 Score = 27.4 bits (59), Expect = 3.5, Method: Composition-based stats. Identities = 13/44 (29%), Positives = 21/44 (47%) Query: 10 FLSSLMFLLLIGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFA 53 FL+ + +LL + IT+S + T P + LN +FA Sbjct: 84 FLTEIGYLLPEPDDFTITTSGVDAEITTTAGPQLVVPVLNARFA 127 >4BD9-B nrPDB Length = 165 Score = 27.4 bits (59), Expect = 3.9, Method: Composition-based stats. Identities = 8/24 (33%), Positives = 12/24 (50%) Query: 27 TSSEMSTICDKTLNPSFCLKFLNT 50 T +E +CD+ P FC + T Sbjct: 98 TETECQEVCDRYQKPGFCYQPSET 121 >6AYO-A nrPDB Length = 238 Score = 27.0 bits (58), Expect = 5.0, Method: Composition-based stats. Identities = 10/54 (18%), Positives = 23/54 (42%) Query: 29 SEMSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDSTQARATQTLKKLQSI 82 + ++ +CD P F L+ ++ K + ++S + A LK + + Sbjct: 185 ASVALVCDALKVPCFILRAMSDKAGEKAEFDFDEFVINSAKISANFVLKMCEKL 238 >5BQN-A nrPDB Length = 885 Score = 26.6 bits (57), Expect = 5.6, Method: Composition-based stats. Identities = 30/146 (20%), Positives = 58/146 (39%), Gaps = 22/146 (15%) Query: 47 FLNTKFASPNLQALAKTTLDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAI 106 FL F + AL T S+ + +K +++ ++ V R K +Y+ V + S I Sbjct: 664 FLLEGFPEFTIPALGVFTFYSSIQEREKIIKTIENCLEQRVK-RWKDSYQWMVSNWLSRI 722 Query: 107 GNLEEAFEHLASGDGMGMNMKVSA----------ALDGAD--TCLDDVKRLRS-----VD 149 F H+ ++ + A G+D V+ L++ + Sbjct: 723 TT---QFNHINYQMYDSLSYQADAIKAKIDLEYKKYSGSDKENIKSQVENLKNSLDVKIS 779 Query: 150 SSVVNNSKTIKNLCGIALVISNMLPR 175 ++ N +K I+ C + + NMLP+ Sbjct: 780 EAMNNINKFIRE-CSVTYLFKNMLPK 804 >5BQM-B nrPDB Length = 474 Score = 26.2 bits (56), Expect = 7.2, Method: Composition-based stats. Identities = 30/146 (20%), Positives = 58/146 (39%), Gaps = 22/146 (15%) Query: 47 FLNTKFASPNLQALAKTTLDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAI 106 FL F + AL T S+ + +K +++ ++ V R K +Y+ V + S I Sbjct: 267 FLLEGFPEFTIPALGVFTFYSSIQEREKIIKTIENCLEQRVK-RWKDSYQWMVSNWLSRI 325 Query: 107 GNLEEAFEHLASGDGMGMNMKVSA----------ALDGAD--TCLDDVKRLRS-----VD 149 F H+ ++ + A G+D V+ L++ + Sbjct: 326 TT---QFNHINYQMYDSLSYQADAIKAKIDLEYKKYSGSDKENIKSQVENLKNSLDVKIS 382 Query: 150 SSVVNNSKTIKNLCGIALVISNMLPR 175 ++ N +K I+ C + + NMLP+ Sbjct: 383 EAMNNINKFIRE-CSVTYLFKNMLPK 407 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.312 0.131 0.337 Lambda K H 0.267 0.0402 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,441,128 Number of Sequences: 36641 Number of extensions: 175961 Number of successful extensions: 548 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 536 Number of HSP's gapped (non-prelim): 20 length of query: 176 length of database: 10,336,785 effective HSP length: 91 effective length of query: 85 effective length of database: 7,002,454 effective search space: 595208590 effective search space used: 595208590 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.4 bits) S2: 56 (26.2 bits)