BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9HVD1 (173 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2ERV-A nrPDB 306 4e-84 4KPA-A nrPDB 30 0.67 5O9A-A nrPDB 28 2.1 4R9I-A nrPDB 26 7.5 4ZGV-A nrPDB 26 9.3 >2ERV-A nrPDB Length = 150 Score = 306 bits (783), Expect = 4e-84, Method: Compositional matrix adjust. Identities = 150/150 (100%), Positives = 150/150 (100%) Query: 24 ADVSAAVGATGQSGMTYRLGLSWDWDKSWWQTSTGRLTGYWDAGYTYWEGGDEGAGKHSL 83 ADVSAAVGATGQSGMTYRLGLSWDWDKSWWQTSTGRLTGYWDAGYTYWEGGDEGAGKHSL Sbjct: 1 ADVSAAVGATGQSGMTYRLGLSWDWDKSWWQTSTGRLTGYWDAGYTYWEGGDEGAGKHSL 60 Query: 84 SFAPVFVYEFAGDSIKPFIEAGIGVAAFSGTRVGDQNLGSSLNFEDRIGAGLKFANGQSV 143 SFAPVFVYEFAGDSIKPFIEAGIGVAAFSGTRVGDQNLGSSLNFEDRIGAGLKFANGQSV Sbjct: 61 SFAPVFVYEFAGDSIKPFIEAGIGVAAFSGTRVGDQNLGSSLNFEDRIGAGLKFANGQSV 120 Query: 144 GVRAIHYSNAGLKQPNDGIESYSLFYKIPI 173 GVRAIHYSNAGLKQPNDGIESYSLFYKIPI Sbjct: 121 GVRAIHYSNAGLKQPNDGIESYSLFYKIPI 150 >4KPA-A nrPDB Length = 494 Score = 29.6 bits (65), Expect = 0.67, Method: Compositional matrix adjust. Identities = 16/43 (37%), Positives = 22/43 (51%) Query: 56 STGRLTGYWDAGYTYWEGGDEGAGKHSLSFAPVFVYEFAGDSI 98 + GR+T Y + E DE +LS A FV +FAGD + Sbjct: 68 APGRVTRYLSSQRLIKEACDESRFDKNLSQALKFVRDFAGDGL 110 >5O9A-A nrPDB Length = 264 Score = 28.1 bits (61), Expect = 2.1, Method: Compositional matrix adjust. Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 5/60 (8%) Query: 62 GYWDAGYTYWEG--GDEGAGKHSLSFAPV---FVYEFAGDSIKPFIEAGIGVAAFSGTRV 116 G DA W G G+ GK ++ AP+ +V E D KPF+ GI + GT + Sbjct: 62 GARDADTKIWNGMVGELVYGKADIAIAPLTITYVREEVIDFSKPFMSLGISIMIKKGTPI 121 >4R9I-A nrPDB Length = 378 Score = 26.2 bits (56), Expect = 7.5, Method: Compositional matrix adjust. Identities = 17/58 (29%), Positives = 28/58 (48%), Gaps = 8/58 (13%) Query: 94 AGDSIKPFIEAGIGVAAFSGTRVGDQNLGSSLNFEDRIGAGLKFANGQSVGVRAIHYS 151 A DS +P+ + I A GT+ Q S GAG K+A +++G++ +S Sbjct: 184 ASDSYRPYKQIDISFTALDGTQSNKQAWYSE-------GAG-KYAEIENLGIKVFQFS 233 >4ZGV-A nrPDB Length = 868 Score = 25.8 bits (55), Expect = 9.3, Method: Compositional matrix adjust. Identities = 20/67 (29%), Positives = 28/67 (41%), Gaps = 9/67 (13%) Query: 10 LAALSSVHVASAQAADVSAAVGATGQSGMTYRLGLSWDWDKSWWQTSTGRLTGYWDAGYT 69 + + S H + DV G SG YR + W+ + SW G++T AGY Sbjct: 314 MTGMYSPHESKFYKKDVKN--GGFTNSGGGYRFNMEWEHNASW-----GKMTSL--AGYQ 364 Query: 70 YWEGGDE 76 Y E E Sbjct: 365 YTEDKTE 371 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2ERV-A nrPDB 301 7e-83 Sequences not found previously or not previously below threshold: 4ZGV-A nrPDB 29 0.92 5O9A-A nrPDB 29 1.3 3PGB-A nrPDB 28 2.0 4R9I-A nrPDB 28 2.5 5DQN-A nrPDB 28 2.7 4KPA-A nrPDB 27 3.2 2XFG-A nrPDB 27 5.0 4BUM-X nrPDB 27 5.6 2AX4-A nrPDB 27 5.6 3DDY-A nrPDB 27 6.2 CONVERGED! >2ERV-A nrPDB Length = 150 Score = 301 bits (772), Expect = 7e-83, Method: Composition-based stats. Identities = 150/150 (100%), Positives = 150/150 (100%) Query: 24 ADVSAAVGATGQSGMTYRLGLSWDWDKSWWQTSTGRLTGYWDAGYTYWEGGDEGAGKHSL 83 ADVSAAVGATGQSGMTYRLGLSWDWDKSWWQTSTGRLTGYWDAGYTYWEGGDEGAGKHSL Sbjct: 1 ADVSAAVGATGQSGMTYRLGLSWDWDKSWWQTSTGRLTGYWDAGYTYWEGGDEGAGKHSL 60 Query: 84 SFAPVFVYEFAGDSIKPFIEAGIGVAAFSGTRVGDQNLGSSLNFEDRIGAGLKFANGQSV 143 SFAPVFVYEFAGDSIKPFIEAGIGVAAFSGTRVGDQNLGSSLNFEDRIGAGLKFANGQSV Sbjct: 61 SFAPVFVYEFAGDSIKPFIEAGIGVAAFSGTRVGDQNLGSSLNFEDRIGAGLKFANGQSV 120 Query: 144 GVRAIHYSNAGLKQPNDGIESYSLFYKIPI 173 GVRAIHYSNAGLKQPNDGIESYSLFYKIPI Sbjct: 121 GVRAIHYSNAGLKQPNDGIESYSLFYKIPI 150 >4ZGV-A nrPDB Length = 868 Score = 29.3 bits (64), Expect = 0.92, Method: Composition-based stats. Identities = 20/67 (29%), Positives = 28/67 (41%), Gaps = 9/67 (13%) Query: 10 LAALSSVHVASAQAADVSAAVGATGQSGMTYRLGLSWDWDKSWWQTSTGRLTGYWDAGYT 69 + + S H + DV G SG YR + W+ + SW G++T AGY Sbjct: 314 MTGMYSPHESKFYKKDVKN--GGFTNSGGGYRFNMEWEHNASW-----GKMTSL--AGYQ 364 Query: 70 YWEGGDE 76 Y E E Sbjct: 365 YTEDKTE 371 >5O9A-A nrPDB Length = 264 Score = 28.9 bits (63), Expect = 1.3, Method: Composition-based stats. Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 5/60 (8%) Query: 62 GYWDAGYTYWEG--GDEGAGKHSLSFAPV---FVYEFAGDSIKPFIEAGIGVAAFSGTRV 116 G DA W G G+ GK ++ AP+ +V E D KPF+ GI + GT + Sbjct: 62 GARDADTKIWNGMVGELVYGKADIAIAPLTITYVREEVIDFSKPFMSLGISIMIKKGTPI 121 >3PGB-A nrPDB Length = 797 Score = 28.1 bits (61), Expect = 2.0, Method: Composition-based stats. Identities = 14/56 (25%), Positives = 27/56 (48%), Gaps = 1/56 (1%) Query: 33 TGQSGMTYRLGLSWDWDKSWWQTSTGRLTGYWDAGYTYWEGGDEGAGKHSLSFAPV 88 TG+ ++L L W ++ +++T+ YW G+ + +GA H+ PV Sbjct: 265 TGRDPSQWKL-LGWMYNDIFYETTEEFRKAYWSPGFVKLKPNVDGAWAHTEQRGPV 319 >4R9I-A nrPDB Length = 378 Score = 27.7 bits (60), Expect = 2.5, Method: Composition-based stats. Identities = 18/63 (28%), Positives = 30/63 (47%), Gaps = 8/63 (12%) Query: 89 FVYEFAGDSIKPFIEAGIGVAAFSGTRVGDQNLGSSLNFEDRIGAGLKFANGQSVGVRAI 148 F + A DS +P+ + I A GT+ Q S GAG K+A +++G++ Sbjct: 179 FPWGEASDSYRPYKQIDISFTALDGTQSNKQAWYSE-------GAG-KYAEIENLGIKVF 230 Query: 149 HYS 151 +S Sbjct: 231 QFS 233 >5DQN-A nrPDB Length = 426 Score = 27.7 bits (60), Expect = 2.7, Method: Composition-based stats. Identities = 13/29 (44%), Positives = 16/29 (55%) Query: 59 RLTGYWDAGYTYWEGGDEGAGKHSLSFAP 87 RL W G +W+ GAGK S+S AP Sbjct: 392 RLKSGWLNGIKHWQVDYTGAGKASVSGAP 420 >4KPA-A nrPDB Length = 494 Score = 27.3 bits (59), Expect = 3.2, Method: Composition-based stats. Identities = 16/43 (37%), Positives = 22/43 (51%) Query: 56 STGRLTGYWDAGYTYWEGGDEGAGKHSLSFAPVFVYEFAGDSI 98 + GR+T Y + E DE +LS A FV +FAGD + Sbjct: 68 APGRVTRYLSSQRLIKEACDESRFDKNLSQALKFVRDFAGDGL 110 >2XFG-A nrPDB Length = 466 Score = 27.0 bits (58), Expect = 5.0, Method: Composition-based stats. Identities = 12/39 (30%), Positives = 15/39 (38%) Query: 48 WDKSWWQTSTGRLTGYWDAGYTYWEGGDEGAGKHSLSFA 86 W W T + Y+ WE GD+ K L FA Sbjct: 330 WLDQWGSLRYATTTAFLACVYSDWENGDKEKAKTYLEFA 368 >4BUM-X nrPDB Length = 289 Score = 26.6 bits (57), Expect = 5.6, Method: Composition-based stats. Identities = 10/20 (50%), Positives = 13/20 (65%) Query: 118 DQNLGSSLNFEDRIGAGLKF 137 D LG+ +N ED+I GLK Sbjct: 78 DNTLGTEINIEDQIAKGLKL 97 >2AX4-A nrPDB Length = 198 Score = 26.6 bits (57), Expect = 5.6, Method: Composition-based stats. Identities = 15/51 (29%), Positives = 24/51 (47%), Gaps = 8/51 (15%) Query: 67 GYTYWEGGDEGAGKHSLSFA--------PVFVYEFAGDSIKPFIEAGIGVA 109 G T W G GAGK ++SFA + Y GD+++ + +G + Sbjct: 22 GCTVWLTGLSGAGKTTISFALEEYLVSHAIPCYSLDGDNVRHGLNRNLGFS 72 >3DDY-A nrPDB Length = 186 Score = 26.6 bits (57), Expect = 6.2, Method: Composition-based stats. Identities = 21/68 (30%), Positives = 30/68 (44%), Gaps = 2/68 (2%) Query: 90 VYEFAGDSIKPFIEAGIGVAAFSGTRVGDQ-NLGSSLNFEDRIGAGLKFANGQSVG-VRA 147 V GD + I+ +G F G + GDQ NL F + +G G N + V A Sbjct: 51 VVRILGDMVYFDIDQALGTTTFDGLKEGDQVNLEIHPKFGEVVGRGGLTGNIKGTALVAA 110 Query: 148 IHYSNAGL 155 I ++AG Sbjct: 111 IEENDAGF 118 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.134 0.413 Lambda K H 0.267 0.0408 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,645,923 Number of Sequences: 36641 Number of extensions: 347187 Number of successful extensions: 888 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 19 Number of HSP's that attempted gapping in prelim test: 874 Number of HSP's gapped (non-prelim): 33 length of query: 173 length of database: 10,336,785 effective HSP length: 90 effective length of query: 83 effective length of database: 7,039,095 effective search space: 584244885 effective search space used: 584244885 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 55 (25.8 bits)