BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9HD47 (186 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5YFG-A nrPDB 380 e-106 3NOK-A nrPDB 28 2.4 3DQV-C nrPDB 28 2.6 >5YFG-A nrPDB Length = 194 Score = 380 bits (975), Expect = e-106, Method: Compositional matrix adjust. Identities = 186/186 (100%), Positives = 186/186 (100%) Query: 1 MEPTRDCPLFGGAFSAILPMGAIDVSDLRPVPDNQEVFCHPVTDQSLIVELLELQAHVRG 60 MEPTRDCPLFGGAFSAILPMGAIDVSDLRPVPDNQEVFCHPVTDQSLIVELLELQAHVRG Sbjct: 1 MEPTRDCPLFGGAFSAILPMGAIDVSDLRPVPDNQEVFCHPVTDQSLIVELLELQAHVRG 60 Query: 61 EAAARYHFEDVGGVQGARAVHVESVQPLSLENLALRGRCQEAWVLSGKQQIAKENQQVAK 120 EAAARYHFEDVGGVQGARAVHVESVQPLSLENLALRGRCQEAWVLSGKQQIAKENQQVAK Sbjct: 61 EAAARYHFEDVGGVQGARAVHVESVQPLSLENLALRGRCQEAWVLSGKQQIAKENQQVAK 120 Query: 121 DVTLHQALLRLPQYQTDLLLTFNQPPPDNRSSLGPENLSPAPWSLGDFEQLVTSLTLHDP 180 DVTLHQALLRLPQYQTDLLLTFNQPPPDNRSSLGPENLSPAPWSLGDFEQLVTSLTLHDP Sbjct: 121 DVTLHQALLRLPQYQTDLLLTFNQPPPDNRSSLGPENLSPAPWSLGDFEQLVTSLTLHDP 180 Query: 181 NIFGPQ 186 NIFGPQ Sbjct: 181 NIFGPQ 186 >3NOK-A nrPDB Length = 268 Score = 28.1 bits (61), Expect = 2.4, Method: Compositional matrix adjust. Identities = 24/82 (29%), Positives = 36/82 (43%), Gaps = 3/82 (3%) Query: 16 AILPMGAIDVSDLRPVPDNQEVFCHPVTDQSLIVELL--ELQAHVRGEAAARYHFEDVGG 73 ++P G+ RPVPD PV + I+ A +G + HF + G Sbjct: 13 GLVPRGSHMSPTNRPVPDAGGSVQEPVRRVAHIIREYPHATNAFTQGLVFHQGHFFESTG 72 Query: 74 VQGA-RAVHVESVQPLSLENLA 94 QG R + +ES QP+ +E L Sbjct: 73 HQGTLRQLSLESAQPVWMERLG 94 >3DQV-C nrPDB Length = 382 Score = 28.1 bits (61), Expect = 2.6, Method: Compositional matrix adjust. Identities = 19/69 (27%), Positives = 30/69 (43%), Gaps = 14/69 (20%) Query: 129 LRLPQYQTDLLLTFNQPPPDNRSSLGPENLSPAP-----------WSLGDFEQLVTSLTL 177 L + +Q +L +NQ P R + ENL A WSL F +L + L Sbjct: 195 LEVTTFQLAVLFAWNQRP---REKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLL 251 Query: 178 HDPNIFGPQ 186 ++P + P+ Sbjct: 252 YEPQVNSPK 260 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5YFG-A nrPDB 392 e-110 Sequences not found previously or not previously below threshold: 3NOK-A nrPDB 30 0.72 3DQV-C nrPDB 28 2.5 5JM7-A nrPDB 28 3.1 3Q48-A nrPDB 28 3.1 CONVERGED! >5YFG-A nrPDB Length = 194 Score = 392 bits (1007), Expect = e-110, Method: Composition-based stats. Identities = 186/186 (100%), Positives = 186/186 (100%) Query: 1 MEPTRDCPLFGGAFSAILPMGAIDVSDLRPVPDNQEVFCHPVTDQSLIVELLELQAHVRG 60 MEPTRDCPLFGGAFSAILPMGAIDVSDLRPVPDNQEVFCHPVTDQSLIVELLELQAHVRG Sbjct: 1 MEPTRDCPLFGGAFSAILPMGAIDVSDLRPVPDNQEVFCHPVTDQSLIVELLELQAHVRG 60 Query: 61 EAAARYHFEDVGGVQGARAVHVESVQPLSLENLALRGRCQEAWVLSGKQQIAKENQQVAK 120 EAAARYHFEDVGGVQGARAVHVESVQPLSLENLALRGRCQEAWVLSGKQQIAKENQQVAK Sbjct: 61 EAAARYHFEDVGGVQGARAVHVESVQPLSLENLALRGRCQEAWVLSGKQQIAKENQQVAK 120 Query: 121 DVTLHQALLRLPQYQTDLLLTFNQPPPDNRSSLGPENLSPAPWSLGDFEQLVTSLTLHDP 180 DVTLHQALLRLPQYQTDLLLTFNQPPPDNRSSLGPENLSPAPWSLGDFEQLVTSLTLHDP Sbjct: 121 DVTLHQALLRLPQYQTDLLLTFNQPPPDNRSSLGPENLSPAPWSLGDFEQLVTSLTLHDP 180 Query: 181 NIFGPQ 186 NIFGPQ Sbjct: 181 NIFGPQ 186 >3NOK-A nrPDB Length = 268 Score = 29.6 bits (65), Expect = 0.72, Method: Composition-based stats. Identities = 24/82 (29%), Positives = 36/82 (43%), Gaps = 3/82 (3%) Query: 16 AILPMGAIDVSDLRPVPDNQEVFCHPVTDQSLIVELL--ELQAHVRGEAAARYHFEDVGG 73 ++P G+ RPVPD PV + I+ A +G + HF + G Sbjct: 13 GLVPRGSHMSPTNRPVPDAGGSVQEPVRRVAHIIREYPHATNAFTQGLVFHQGHFFESTG 72 Query: 74 VQGA-RAVHVESVQPLSLENLA 94 QG R + +ES QP+ +E L Sbjct: 73 HQGTLRQLSLESAQPVWMERLG 94 >3DQV-C nrPDB Length = 382 Score = 28.1 bits (61), Expect = 2.5, Method: Composition-based stats. Identities = 19/69 (27%), Positives = 30/69 (43%), Gaps = 14/69 (20%) Query: 129 LRLPQYQTDLLLTFNQPPPDNRSSLGPENLSPAP-----------WSLGDFEQLVTSLTL 177 L + +Q +L +NQ P R + ENL A WSL F +L + L Sbjct: 195 LEVTTFQLAVLFAWNQRP---REKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLL 251 Query: 178 HDPNIFGPQ 186 ++P + P+ Sbjct: 252 YEPQVNSPK 260 >5JM7-A nrPDB Length = 576 Score = 27.7 bits (60), Expect = 3.1, Method: Composition-based stats. Identities = 20/81 (24%), Positives = 34/81 (41%), Gaps = 2/81 (2%) Query: 73 GVQGARAVHVESVQPLSLENLALRGRCQEAWVLSGKQQIAKENQQVAKDVTLHQALLRLP 132 G++ AR + Q L R ++ W +G + + Q + L+ P Sbjct: 300 GMRLARLAQTDDWQTLQARFPTFRVMQEDGW--AGLRDLHGNIMQESLFALRENLLVDQP 357 Query: 133 QYQTDLLLTFNQPPPDNRSSL 153 Q QT++L++ Q PD SL Sbjct: 358 QSQTNVLVSLTQAAPDGGDSL 378 >3Q48-A nrPDB Length = 257 Score = 27.7 bits (60), Expect = 3.1, Method: Composition-based stats. Identities = 27/92 (29%), Positives = 42/92 (45%), Gaps = 6/92 (6%) Query: 75 QGARAVHVESVQPLSLE--NLALRGRCQEAWVLSGKQQIAKENQQVAKDVTLHQALLRL- 131 QG R V S + ++L N + +AW+ G+Q + E QV VT A+ R+ Sbjct: 33 QGTRVVFPASEREVTLRVSNTSGTPVLAQAWIDDGRQDVPPEELQVPFSVT--PAVTRVE 90 Query: 132 PQYQTDLLLTFNQPP-PDNRSSLGPENLSPAP 162 P L + + + P P +R SL N+ P Sbjct: 91 PNGGAVLRIAYLKAPLPTDRESLFWLNILEVP 122 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.136 0.409 Lambda K H 0.267 0.0417 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,639,233 Number of Sequences: 36641 Number of extensions: 291417 Number of successful extensions: 602 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 600 Number of HSP's gapped (non-prelim): 12 length of query: 186 length of database: 10,336,785 effective HSP length: 91 effective length of query: 95 effective length of database: 7,002,454 effective search space: 665233130 effective search space used: 665233130 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)