BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= Q9HBE4
         (162 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

2OQP-A nrPDB                                                          280   1e-76
5VI4-B nrPDB                                                           32   0.11 
3JAQ-j nrPDB                                                           31   0.25 
3TX1-A nrPDB                                                           28   1.2  
3NF5-A nrPDB                                                           28   2.2  
4BM1-A nrPDB                                                           27   4.9  
4KC3-B nrPDB                                                           26   6.0  

>2OQP-A nrPDB
          Length = 134

 Score =  280 bits (717), Expect = 1e-76,   Method: Compositional matrix adjust.
 Identities = 133/133 (100%), Positives = 133/133 (100%)

Query: 30  QGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANT 89
           QGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANT
Sbjct: 2   QGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANT 61

Query: 90  GNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIH 149
           GNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIH
Sbjct: 62  GNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIH 121

Query: 150 QHLSSRTHGSEDS 162
           QHLSSRTHGSEDS
Sbjct: 122 QHLSSRTHGSEDS 134


>5VI4-B nrPDB
          Length = 309

 Score = 32.3 bits (72), Expect = 0.11,   Method: Compositional matrix adjust.
 Identities = 21/73 (28%), Positives = 32/73 (43%), Gaps = 13/73 (17%)

Query: 91  NNERIINVSIKKLKRKPPSTNA----------GRRQKHRLTCPSCDSYEKKPPKEFLERF 140
           N    +NV+I K   KPPS N           G  +  ++TCP+ D Y    P ++ +  
Sbjct: 78  NKTGYLNVTIHK---KPPSCNIPDYLMYSTVRGSDKNFKITCPTIDLYNWTAPVQWFKNC 134

Query: 141 KSLLQKMIHQHLS 153
           K+L +     H S
Sbjct: 135 KALQEPRFRAHRS 147


>3JAQ-j nrPDB
          Length = 304

 Score = 30.8 bits (68), Expect = 0.25,   Method: Compositional matrix adjust.
 Identities = 18/54 (33%), Positives = 31/54 (57%), Gaps = 3/54 (5%)

Query: 44  DIVDQLKNYVND-LVPEFLPAPEDVETNC-EWSAFSCFQKAQLKSANTGNNERI 95
           D++D+LKNY++  L P+ +    DVE +C  +      + A LKSA   + E++
Sbjct: 164 DVLDELKNYISKRLTPQAVKIRADVEVSCFSYEGIDAIKDA-LKSAEDMSTEQM 216


>3TX1-A nrPDB
          Length = 322

 Score = 28.5 bits (62), Expect = 1.2,   Method: Compositional matrix adjust.
 Identities = 13/38 (34%), Positives = 17/38 (44%)

Query: 103 LKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERF 140
           ++ K     A R  K  L  PSC S  K+PP  F  + 
Sbjct: 220 IQAKMDELTAAREAKQPLEYPSCGSVFKRPPGHFAGKL 257


>3NF5-A nrPDB
          Length = 164

 Score = 27.7 bits (60), Expect = 2.2,   Method: Compositional matrix adjust.
 Identities = 17/59 (28%), Positives = 27/59 (45%), Gaps = 7/59 (11%)

Query: 108 PSTNAGRRQKHRLTCPSC---DSYEKKPPKE----FLERFKSLLQKMIHQHLSSRTHGS 159
           P    G   + R++C +C   D   +KP K+     +ER+   L+K+ H H  S    S
Sbjct: 87  PEKGKGLNVRARISCYNCYPLDKSTRKPIKDPNHRIMERYSEKLKKIPHTHFESYDPAS 145


>4BM1-A nrPDB
          Length = 338

 Score = 26.6 bits (57), Expect = 4.9,   Method: Compositional matrix adjust.
 Identities = 19/65 (29%), Positives = 32/65 (49%), Gaps = 13/65 (20%)

Query: 66  DVETNCEWSAFSC--------FQKAQLKSANTGNN-ERIINVS----IKKLKRKPPSTNA 112
           D  T+CEW +F          F++A  K A  G N +++I+ S    + + + K P+  A
Sbjct: 244 DSRTSCEWQSFISNPNSMVPKFERAMAKMATLGQNPKKLIDCSEVIPVPRGRVKQPTLPA 303

Query: 113 GRRQK 117
           G+  K
Sbjct: 304 GKTIK 308


>4KC3-B nrPDB
          Length = 309

 Score = 26.2 bits (56), Expect = 6.0,   Method: Compositional matrix adjust.
 Identities = 12/45 (26%), Positives = 21/45 (46%)

Query: 109 STNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLS 153
           ST +G  +  ++ CP+ D Y    P E+ +  ++L       H S
Sbjct: 102 STVSGSEKNSKIYCPTIDLYNWTAPLEWFKNCQALQGSRYRAHKS 146


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

2OQP-A nrPDB                                                          280   3e-76

Sequences not found previously or not previously below threshold:

5VI4-B nrPDB                                                           34   0.037
3NF5-A nrPDB                                                           31   0.29 
3JAQ-j nrPDB                                                           30   0.32 
4BM1-A nrPDB                                                           29   1.3  
3TX1-A nrPDB                                                           28   1.6  
4Q9V-A nrPDB                                                           28   2.5  
6CP5-Z nrPDB                                                           27   4.2  
4KC3-B nrPDB                                                           26   7.0  


CONVERGED!
>2OQP-A nrPDB
          Length = 134

 Score =  280 bits (715), Expect = 3e-76,   Method: Composition-based stats.
 Identities = 133/133 (100%), Positives = 133/133 (100%)

Query: 30  QGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANT 89
           QGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANT
Sbjct: 2   QGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANT 61

Query: 90  GNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIH 149
           GNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIH
Sbjct: 62  GNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIH 121

Query: 150 QHLSSRTHGSEDS 162
           QHLSSRTHGSEDS
Sbjct: 122 QHLSSRTHGSEDS 134


>5VI4-B nrPDB
          Length = 309

 Score = 33.9 bits (76), Expect = 0.037,   Method: Composition-based stats.
 Identities = 30/105 (28%), Positives = 47/105 (44%), Gaps = 23/105 (21%)

Query: 59  EFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNA------ 112
           +FLPA   VE +     ++C     ++S N  N    +NV+I K   KPPS N       
Sbjct: 56  KFLPA--RVEDS---GIYACV----IRSPNL-NKTGYLNVTIHK---KPPSCNIPDYLMY 102

Query: 113 ----GRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLS 153
               G  +  ++TCP+ D Y    P ++ +  K+L +     H S
Sbjct: 103 STVRGSDKNFKITCPTIDLYNWTAPVQWFKNCKALQEPRFRAHRS 147


>3NF5-A nrPDB
          Length = 164

 Score = 30.8 bits (68), Expect = 0.29,   Method: Composition-based stats.
 Identities = 16/54 (29%), Positives = 26/54 (48%), Gaps = 7/54 (12%)

Query: 108 PSTNAGRRQKHRLTCPSC---DSYEKKPPKE----FLERFKSLLQKMIHQHLSS 154
           P    G   + R++C +C   D   +KP K+     +ER+   L+K+ H H  S
Sbjct: 87  PEKGKGLNVRARISCYNCYPLDKSTRKPIKDPNHRIMERYSEKLKKIPHTHFES 140


>3JAQ-j nrPDB
          Length = 304

 Score = 30.4 bits (67), Expect = 0.32,   Method: Composition-based stats.
 Identities = 18/54 (33%), Positives = 31/54 (57%), Gaps = 3/54 (5%)

Query: 44  DIVDQLKNYVND-LVPEFLPAPEDVETNC-EWSAFSCFQKAQLKSANTGNNERI 95
           D++D+LKNY++  L P+ +    DVE +C  +      + A LKSA   + E++
Sbjct: 164 DVLDELKNYISKRLTPQAVKIRADVEVSCFSYEGIDAIKDA-LKSAEDMSTEQM 216


>4BM1-A nrPDB
          Length = 338

 Score = 28.5 bits (62), Expect = 1.3,   Method: Composition-based stats.
 Identities = 19/65 (29%), Positives = 32/65 (49%), Gaps = 13/65 (20%)

Query: 66  DVETNCEWSAFSC--------FQKAQLKSANTGNN-ERIINVS----IKKLKRKPPSTNA 112
           D  T+CEW +F          F++A  K A  G N +++I+ S    + + + K P+  A
Sbjct: 244 DSRTSCEWQSFISNPNSMVPKFERAMAKMATLGQNPKKLIDCSEVIPVPRGRVKQPTLPA 303

Query: 113 GRRQK 117
           G+  K
Sbjct: 304 GKTIK 308


>3TX1-A nrPDB
          Length = 322

 Score = 28.1 bits (61), Expect = 1.6,   Method: Composition-based stats.
 Identities = 13/34 (38%), Positives = 16/34 (47%)

Query: 103 LKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEF 136
           ++ K     A R  K  L  PSC S  K+PP  F
Sbjct: 220 IQAKMDELTAAREAKQPLEYPSCGSVFKRPPGHF 253


>4Q9V-A nrPDB
          Length = 186

 Score = 27.7 bits (60), Expect = 2.5,   Method: Composition-based stats.
 Identities = 9/32 (28%), Positives = 19/32 (59%)

Query: 127 SYEKKPPKEFLERFKSLLQKMIHQHLSSRTHG 158
           ++++      L   K L+ +++ +HL+ RTHG
Sbjct: 110 TFDRNVLSNLLHECKDLVHELVQRHLTPRTHG 141


>6CP5-Z nrPDB
          Length = 209

 Score = 27.0 bits (58), Expect = 4.2,   Method: Composition-based stats.
 Identities = 26/118 (22%), Positives = 54/118 (45%), Gaps = 7/118 (5%)

Query: 32  QDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGN 91
           +++H+  ++  ID V QL+N        F  + E VE   E  AF   QK +L       
Sbjct: 96  RNKHVEAVKDRIDSVSQLQNVAETTKVLFDVSKETVELESE--AFELKQKVELAHEAKAV 153

Query: 92  NERII--NVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKM 147
            +  +    S+++L+++  + +   R +  L  P    +++K  ++ +   + LL K+
Sbjct: 154 LDSWVRYEASLRQLEQRQLAKSVISRVQSELGNP---KFQEKVLQQSISEIEQLLSKL 208


>4KC3-B nrPDB
          Length = 309

 Score = 26.2 bits (56), Expect = 7.0,   Method: Composition-based stats.
 Identities = 12/45 (26%), Positives = 21/45 (46%)

Query: 109 STNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLS 153
           ST +G  +  ++ CP+ D Y    P E+ +  ++L       H S
Sbjct: 102 STVSGSEKNSKIYCPTIDLYNWTAPLEWFKNCQALQGSRYRAHKS 146


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.319    0.132    0.390 

Lambda     K      H
   0.267   0.0405    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,557,149
Number of Sequences: 36641
Number of extensions: 250371
Number of successful extensions: 684
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 668
Number of HSP's gapped (non-prelim): 24
length of query: 162
length of database: 10,336,785
effective HSP length: 89
effective length of query: 73
effective length of database: 7,075,736
effective search space: 516528728
effective search space used: 516528728
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)