BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9HAB8 (311 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1P9O-A nrPDB 622 e-179 6AI8-A nrPDB 187 5e-48 5INT-A nrPDB 40 0.001 1U7Z-A nrPDB 30 1.1 1BOH-A nrPDB 28 4.4 2KQY-A nrPDB 28 5.6 >1P9O-A nrPDB Length = 313 Score = 622 bits (1604), Expect = e-179, Method: Compositional matrix adjust. Identities = 308/311 (99%), Positives = 309/311 (99%) Query: 1 MAEMDPVAEFPQPPGAARWAEVMARFAARLGAQGRRVVLVTSGGTKVPLEARPVRFLDNF 60 MAEMDPVAEFPQPPGAARWAEVMARFAARLGAQGRRVVLVTSGGTKVPLEARPVRFLDNF Sbjct: 3 MAEMDPVAEFPQPPGAARWAEVMARFAARLGAQGRRVVLVTSGGTKVPLEARPVRFLDNF 62 Query: 61 SSGRRGATSAEAFLAAGYGVLFLYRARSAFPYAHRFPPQTWLSALRPSGPALSGLLSLEA 120 SSGRRGATSAEAFLAAGYGVLFLYRARSAFPYAHRFPPQTWLSALRPSGPALSGLLSLEA Sbjct: 63 SSGRRGATSAEAFLAAGYGVLFLYRARSAFPYAHRFPPQTWLSALRPSGPALSGLLSLEA 122 Query: 121 EENALPGFAEALRSYQEAAAAGTFLAVEFTTLADYLHLLQAAAQALNPLGPSAMFYLAAA 180 EENALPGFAEALRSYQEAAAAGTFL VEFTTLADYLHLLQAAAQALNPLGPSAMFYLAAA Sbjct: 123 EENALPGFAEALRSYQEAAAAGTFLVVEFTTLADYLHLLQAAAQALNPLGPSAMFYLAAA 182 Query: 181 VSDFYVPVSEMPEHKIQSSGGPLQITMKMVPKLLSPLVKDWAPKAFIISFKLETDPAIVI 240 VSDFYVPVSEMPEHKI+SSGGPLQITMKMVPKLLSPLVKDWAPKAFIISFKLETDPAIVI Sbjct: 183 VSDFYVPVSEMPEHKIESSGGPLQITMKMVPKLLSPLVKDWAPKAFIISFKLETDPAIVI 242 Query: 241 NRARKALEIYQHQVVVANILESRQSFVFIVTKDSETKLLLSEEEIEKGVEIEEKIVDNLQ 300 NRARKALEIYQHQVVVANILESRQSFV IVTKDSETKLLLSEEEIEKGVEIEEKIVDNLQ Sbjct: 243 NRARKALEIYQHQVVVANILESRQSFVLIVTKDSETKLLLSEEEIEKGVEIEEKIVDNLQ 302 Query: 301 SRHTAFIGDRN 311 SRHTAFIGDRN Sbjct: 303 SRHTAFIGDRN 313 >6AI8-A nrPDB Length = 371 Score = 187 bits (475), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 114/293 (38%), Positives = 162/293 (55%), Gaps = 28/293 (9%) Query: 35 RRVVLVTSGGTKVPLEARPVRFLDNFSSGRRGATSAEAFLAAGYGVLFLYRARSAFPYAH 94 ++VL+TSGGT VPLE VRF+DNFS+G RGA+SAE FLA GY V+FL+R S PY Sbjct: 79 NKIVLITSGGTTVPLENNTVRFIDNFSAGTRGASSAEQFLANGYSVIFLHREFSLTPYNR 138 Query: 95 RFPPQTWLSALRPSGPALSGLLSLEAEENALPGFAEALRSYQEAAAAGTFLAVEFTTLAD 154 F ++ L G + E EN L + Y E L + FTT+ Sbjct: 139 SFSHS--INTLFLDYIDSEGKIKPEFAENVLKN-KKLYDKYMEKEEK--LLLLPFTTVNQ 193 Query: 155 YLHLLQAAAQALNPLGPSAMFYLAAAVSDFYVPVSEMPEHKIQSS--------------- 199 YL L++ A+ LN G +FYLAAAVSDF+VP S +P+HKIQS Sbjct: 194 YLWSLKSIAKLLNNSG--CLFYLAAAVSDFFVPYSRLPQHKIQSGDNGKMGANNDTEGTT 251 Query: 200 ----GGPLQITMKMVPKLLSPLVKDWAPKAFIISFKLETDPAIVINRARKALEIYQHQVV 255 G L + + VPK L LV+ WA +A I+SFKLETD ++++ + +AL+ Y HQ+V Sbjct: 252 RTTPDGKLIVNLDPVPKFLRRLVESWATQAMIVSFKLETDESMLLYKCTQALDRYNHQLV 311 Query: 256 VANILESRQSFVFIVTKDSETKLLLSEEEIEKGVEIEEKIVDNLQSRHTAFIG 308 + N+L++R V V+ ++ + + EK IEE I+ + +RH ++ Sbjct: 312 IGNLLQTRNKQVIFVSPENRKGDWVRLD--EKHASIEEMIIPEVIARHDKWVA 362 >5INT-A nrPDB Length = 220 Score = 40.0 bits (92), Expect = 0.001, Method: Compositional matrix adjust. Identities = 21/51 (41%), Positives = 33/51 (64%), Gaps = 3/51 (5%) Query: 33 QGRRVVLVTSGGTKVPLEARPVRFLDNFSSGRRGATSAEAFLAAGYGVLFL 83 QG+R+ L+T+G T+ ++ PVRF+ NFSSG+ G AE + G V+ + Sbjct: 5 QGKRI-LITAGPTREKID--PVRFMTNFSSGKMGYAIAEVAVNLGAEVILV 52 >1U7Z-A nrPDB Length = 226 Score = 30.4 bits (67), Expect = 1.1, Method: Compositional matrix adjust. Identities = 12/29 (41%), Positives = 22/29 (75%), Gaps = 2/29 (6%) Query: 38 VLVTSGGTKVPLEARPVRFLDNFSSGRRG 66 +++T+G T+ PL+ PVR++ + SSG+ G Sbjct: 11 IMITAGPTREPLD--PVRYISDHSSGKMG 37 >1BOH-A nrPDB Length = 296 Score = 28.1 bits (61), Expect = 4.4, Method: Compositional matrix adjust. Identities = 17/46 (36%), Positives = 26/46 (56%), Gaps = 4/46 (8%) Query: 61 SSGRRGATSAEAFLAA---GYGVLFLYRARSAFPYAHRFPPQTWLS 103 ++ R+G T+ LAA G + +Y S F + HR PP+TW+S Sbjct: 245 ATCRKGVTACHIALAAYLCGKPDVAIYDG-SWFEWFHRAPPETWVS 289 >2KQY-A nrPDB Length = 108 Score = 28.1 bits (61), Expect = 5.6, Method: Compositional matrix adjust. Identities = 17/24 (70%), Positives = 17/24 (70%), Gaps = 2/24 (8%) Query: 56 FLDNFSSGRRGATSAE--AFLAAG 77 FL NFSS R TSAE AFLAAG Sbjct: 66 FLKNFSSSARVLTSAETKAFLAAG 89 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1P9O-A nrPDB 521 e-148 6AI8-A nrPDB 402 e-112 5INT-A nrPDB 71 5e-13 Sequences not found previously or not previously below threshold: 1U7Z-A nrPDB 39 0.002 3VX4-A nrPDB 31 0.56 6DGB-A nrPDB 30 1.1 2REK-A nrPDB 30 1.8 6ADQ-B nrPDB 29 2.1 4B3J-A nrPDB 29 2.1 4B09-A nrPDB 29 3.1 5ZWM-J nrPDB 29 4.0 4MNK-A nrPDB 27 9.3 CONVERGED! >1P9O-A nrPDB Length = 313 Score = 521 bits (1343), Expect = e-148, Method: Composition-based stats. Identities = 308/311 (99%), Positives = 309/311 (99%) Query: 1 MAEMDPVAEFPQPPGAARWAEVMARFAARLGAQGRRVVLVTSGGTKVPLEARPVRFLDNF 60 MAEMDPVAEFPQPPGAARWAEVMARFAARLGAQGRRVVLVTSGGTKVPLEARPVRFLDNF Sbjct: 3 MAEMDPVAEFPQPPGAARWAEVMARFAARLGAQGRRVVLVTSGGTKVPLEARPVRFLDNF 62 Query: 61 SSGRRGATSAEAFLAAGYGVLFLYRARSAFPYAHRFPPQTWLSALRPSGPALSGLLSLEA 120 SSGRRGATSAEAFLAAGYGVLFLYRARSAFPYAHRFPPQTWLSALRPSGPALSGLLSLEA Sbjct: 63 SSGRRGATSAEAFLAAGYGVLFLYRARSAFPYAHRFPPQTWLSALRPSGPALSGLLSLEA 122 Query: 121 EENALPGFAEALRSYQEAAAAGTFLAVEFTTLADYLHLLQAAAQALNPLGPSAMFYLAAA 180 EENALPGFAEALRSYQEAAAAGTFL VEFTTLADYLHLLQAAAQALNPLGPSAMFYLAAA Sbjct: 123 EENALPGFAEALRSYQEAAAAGTFLVVEFTTLADYLHLLQAAAQALNPLGPSAMFYLAAA 182 Query: 181 VSDFYVPVSEMPEHKIQSSGGPLQITMKMVPKLLSPLVKDWAPKAFIISFKLETDPAIVI 240 VSDFYVPVSEMPEHKI+SSGGPLQITMKMVPKLLSPLVKDWAPKAFIISFKLETDPAIVI Sbjct: 183 VSDFYVPVSEMPEHKIESSGGPLQITMKMVPKLLSPLVKDWAPKAFIISFKLETDPAIVI 242 Query: 241 NRARKALEIYQHQVVVANILESRQSFVFIVTKDSETKLLLSEEEIEKGVEIEEKIVDNLQ 300 NRARKALEIYQHQVVVANILESRQSFV IVTKDSETKLLLSEEEIEKGVEIEEKIVDNLQ Sbjct: 243 NRARKALEIYQHQVVVANILESRQSFVLIVTKDSETKLLLSEEEIEKGVEIEEKIVDNLQ 302 Query: 301 SRHTAFIGDRN 311 SRHTAFIGDRN Sbjct: 303 SRHTAFIGDRN 313 >6AI8-A nrPDB Length = 371 Score = 402 bits (1033), Expect = e-112, Method: Composition-based stats. Identities = 114/293 (38%), Positives = 162/293 (55%), Gaps = 28/293 (9%) Query: 35 RRVVLVTSGGTKVPLEARPVRFLDNFSSGRRGATSAEAFLAAGYGVLFLYRARSAFPYAH 94 ++VL+TSGGT VPLE VRF+DNFS+G RGA+SAE FLA GY V+FL+R S PY Sbjct: 79 NKIVLITSGGTTVPLENNTVRFIDNFSAGTRGASSAEQFLANGYSVIFLHREFSLTPYNR 138 Query: 95 RFPPQTWLSALRPSGPALSGLLSLEAEENALPGFAEALRSYQEAAAAGTFLAVEFTTLAD 154 F ++ L G + E EN L + Y E L + FTT+ Sbjct: 139 SFSHS--INTLFLDYIDSEGKIKPEFAENVLKN-KKLYDKYMEKEEK--LLLLPFTTVNQ 193 Query: 155 YLHLLQAAAQALNPLGPSAMFYLAAAVSDFYVPVSEMPEHKIQSSG-------------- 200 YL L++ A+ LN G +FYLAAAVSDF+VP S +P+HKIQS Sbjct: 194 YLWSLKSIAKLLNNSG--CLFYLAAAVSDFFVPYSRLPQHKIQSGDNGKMGANNDTEGTT 251 Query: 201 -----GPLQITMKMVPKLLSPLVKDWAPKAFIISFKLETDPAIVINRARKALEIYQHQVV 255 G L + + VPK L LV+ WA +A I+SFKLETD ++++ + +AL+ Y HQ+V Sbjct: 252 RTTPDGKLIVNLDPVPKFLRRLVESWATQAMIVSFKLETDESMLLYKCTQALDRYNHQLV 311 Query: 256 VANILESRQSFVFIVTKDSETKLLLSEEEIEKGVEIEEKIVDNLQSRHTAFIG 308 + N+L++R V V+ ++ + + EK IEE I+ + +RH ++ Sbjct: 312 IGNLLQTRNKQVIFVSPENRKGDWVRLD--EKHASIEEMIIPEVIARHDKWVA 362 >5INT-A nrPDB Length = 220 Score = 71.3 bits (173), Expect = 5e-13, Method: Composition-based stats. Identities = 23/62 (37%), Positives = 36/62 (58%), Gaps = 3/62 (4%) Query: 33 QGRRVVLVTSGGTKVPLEARPVRFLDNFSSGRRGATSAEAFLAAGYGVLFLYRARSAFPY 92 QG+R+ L+T+G T+ ++ PVRF+ NFSSG+ G AE + G V+ + + P Sbjct: 5 QGKRI-LITAGPTREKID--PVRFMTNFSSGKMGYAIAEVAVNLGAEVILVSGPTALNPP 61 Query: 93 AH 94 H Sbjct: 62 LH 63 >1U7Z-A nrPDB Length = 226 Score = 39.3 bits (90), Expect = 0.002, Method: Composition-based stats. Identities = 16/46 (34%), Positives = 27/46 (58%), Gaps = 2/46 (4%) Query: 38 VLVTSGGTKVPLEARPVRFLDNFSSGRRGATSAEAFLAAGYGVLFL 83 +++T+G T+ PL+ PVR++ + SSG+ G A A G V + Sbjct: 11 IMITAGPTREPLD--PVRYISDHSSGKMGFAIAAAAARRGANVTLV 54 >3VX4-A nrPDB Length = 273 Score = 31.2 bits (69), Expect = 0.56, Method: Composition-based stats. Identities = 12/75 (16%), Positives = 35/75 (46%), Gaps = 5/75 (6%) Query: 232 LETDPAIVINRARKALEIYQHQVVVANILESRQSFVFIVTKDSETKLLLSEEEI---EKG 288 L P ++++ A +L+I + +++N+L+ + + V + +E+ ++G Sbjct: 187 LTQAPVLILDAATSSLDILTEKKIISNLLQMTEKTIIFVA--HRLSISQRTDEVIVMDQG 244 Query: 289 VEIEEKIVDNLQSRH 303 +E+ L ++ Sbjct: 245 KIVEQGTHKELLAKQ 259 >6DGB-A nrPDB Length = 143 Score = 30.4 bits (67), Expect = 1.1, Method: Composition-based stats. Identities = 20/53 (37%), Positives = 25/53 (47%), Gaps = 8/53 (15%) Query: 23 MARFA-----ARLGAQGRRVVLVTSGGTKVPLEARPVRFLDNFSS---GRRGA 67 +ARF A L AQGRR+V+ G T L + L + GRRGA Sbjct: 73 LARFGVEHLEAALSAQGRRIVVADPGETTDDLVCDMIEVLTGMCARLYGRRGA 125 >2REK-A nrPDB Length = 199 Score = 29.7 bits (65), Expect = 1.8, Method: Composition-based stats. Identities = 26/111 (23%), Positives = 37/111 (33%) Query: 8 AEFPQPPGAARWAEVMARFAARLGAQGRRVVLVTSGGTKVPLEARPVRFLDNFSSGRRGA 67 AE P RW +A F A R ++ T T L++R + L + Sbjct: 83 AEHPPATALTRWLTSLAVFGAVTRGAARSLLPATGTNTGAALDSRCEQLLTEAGADLLAR 142 Query: 68 TSAEAFLAAGYGVLFLYRARSAFPYAHRFPPQTWLSALRPSGPALSGLLSL 118 + + L L +A A P A R G AL GL + Sbjct: 143 AQEDGTVRDDVTALELLSLANAVSLAAEHTPDAAHHATRLMGIALGGLGAP 193 >6ADQ-B nrPDB Length = 546 Score = 29.3 bits (64), Expect = 2.1, Method: Composition-based stats. Identities = 29/141 (20%), Positives = 57/141 (40%), Gaps = 22/141 (15%) Query: 79 GVLFLYRARSAFPYAHRFPPQTWLS-------ALRPSGPALSGLLS-LEAEENALPGFAE 130 G++ L+ A +P+ H P W++ AL + P + ++ +A N L + Sbjct: 310 GLIRLWPAWEFYPFGHTIPQGVWVAVGMGLVFALLIAYPFIEKKVTGDDAHHNLLQRPRD 369 Query: 131 A-LRSYQEAAAAGTFLAVEFTTLADYLHL-LQAAAQALNPLGPSAMFYLAAAVSDFYVPV 188 +R+ + A +L + F + D + L + A +G M L A V ++V Sbjct: 370 VPVRTAIGSMAIALYLLLTFACMNDIIALKFHISLNATTWIGRIGMVVLPAIV--YFVAY 427 Query: 189 ----------SEMPEHKIQSS 199 E+ EH +++ Sbjct: 428 RWAISLQRSDREVLEHGVETG 448 >4B3J-A nrPDB Length = 736 Score = 29.3 bits (64), Expect = 2.1, Method: Composition-based stats. Identities = 11/44 (25%), Positives = 21/44 (47%), Gaps = 1/44 (2%) Query: 40 VTSGGTK-VPLEARPVRFLDNFSSGRRGATSAEAFLAAGYGVLF 82 + +GG++ + P++ + +G GA A AGY V+ Sbjct: 326 INAGGSRPEGIGKTPIKRIGVLGAGMMGAGIAYVSAKAGYEVVL 369 >4B09-A nrPDB Length = 240 Score = 28.9 bits (63), Expect = 3.1, Method: Composition-based stats. Identities = 15/67 (22%), Positives = 23/67 (34%) Query: 45 TKVPLEARPVRFLDNFSSGRRGATSAEAFLAAGYGVLFLYRARSAFPYAHRFPPQTWLSA 104 T++P++ R L + G + AA Y + PY + PP L Sbjct: 2 TELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGDQVLPYVRQTPPDLILLD 61 Query: 105 LRPSGPA 111 L G Sbjct: 62 LMLPGTD 68 >5ZWM-J nrPDB Length = 469 Score = 28.5 bits (62), Expect = 4.0, Method: Composition-based stats. Identities = 17/68 (25%), Positives = 29/68 (42%) Query: 242 RARKALEIYQHQVVVANILESRQSFVFIVTKDSETKLLLSEEEIEKGVEIEEKIVDNLQS 301 R + L+ YQ + I + R ++ + KL E+E +K IE + NL+ Sbjct: 85 RYERGLKFYQKGEISKRIAQERTLQKQQEEEELKRKLKQEEDEKDKRKLIESGDLPNLEL 144 Query: 302 RHTAFIGD 309 F+ D Sbjct: 145 HEDKFLLD 152 >4MNK-A nrPDB Length = 348 Score = 27.3 bits (59), Expect = 9.3, Method: Composition-based stats. Identities = 30/156 (19%), Positives = 52/156 (33%), Gaps = 26/156 (16%) Query: 2 AEMD-PVAEFPQPPGAARWAEVMARFAARLGAQGRRVVLVTSGGTKVPLEARPVRFLDNF 60 AE++ P E PPG+ + AE RL + ++ + GG++V DNF Sbjct: 36 AELNAPTFEVRVPPGSEKTAEDFTPTVRRLNPSVKTLISIGGGGSEVR---------DNF 86 Query: 61 SSGRRGATSAEAFLAAGYGVLFLYRARSAFPYAHRFPPQTWLSALRPSGPALSGLLSLEA 120 + A++ + F+ +S+ A R+ L L +E Sbjct: 87 AKLNSDASARQRFV------------KSSIALARRY----GFHGLDLDYEYPEPQLEMEN 130 Query: 121 EENALPGFAEALRSYQEAAAAGTFLAVEFTTLADYL 156 + A+R + L E L Sbjct: 131 FVKLVSELTAAIREEARTSGKPRLLLTEAVYFHQKL 166 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.313 0.133 0.365 Lambda K H 0.267 0.0390 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,758,448 Number of Sequences: 36641 Number of extensions: 377152 Number of successful extensions: 1127 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 1101 Number of HSP's gapped (non-prelim): 23 length of query: 311 length of database: 10,336,785 effective HSP length: 97 effective length of query: 214 effective length of database: 6,782,608 effective search space: 1451478112 effective search space used: 1451478112 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.4 bits) S2: 59 (27.4 bits)