BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9H1P3 (480 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5ZM8-A nrPDB 911 0.0 4B2Z-A nrPDB 90 2e-18 1ZI7-A nrPDB 66 3e-11 3H6Q-A nrPDB 28 8.4 >5ZM8-A nrPDB Length = 455 Score = 911 bits (2354), Expect = 0.0, Method: Compositional matrix adjust. Identities = 433/436 (99%), Positives = 433/436 (99%) Query: 45 GKTGERPSQENGIQKHRTSLPAPMFSRSDFSVWTILKKCVGLELSKITMPIAFNEPLSFL 104 G ERPSQENGIQKHRTSLPAPMFSRSDFSVWTILKKCVGLELSKITMPIAFNEPLSFL Sbjct: 20 GPHMERPSQENGIQKHRTSLPAPMFSRSDFSVWTILKKCVGLELSKITMPIAFNEPLSFL 79 Query: 105 QRITEYMEHVYLIHRASCQPQPLERMQSVAAFAVSAVASQWERTGKPFNPLLGETYELIR 164 QRITEYMEHVYLIHRASCQPQPLERMQSVAAFAVSAVASQWERTGKPFNPLLGETYELIR Sbjct: 80 QRITEYMEHVYLIHRASCQPQPLERMQSVAAFAVSAVASQWERTGKPFNPLLGETYELIR 139 Query: 165 EDLGFRFISEQVSHHPPISAFHSEGLNHDFLFHGSIYPKLKFWGKSVEAEPRGTITLELL 224 EDLGFRFISEQVSHHPPISAFHSEGLNHDFLFHGSIYPKLKFWGKSVEAEPRGTITLELL Sbjct: 140 EDLGFRFISEQVSHHPPISAFHSEGLNHDFLFHGSIYPKLKFWGKSVEAEPRGTITLELL 199 Query: 225 KHNEAYTWTNPTCCVHNVIIGKLWIEQYGTVEILNHRTGHKCVLHFKPCGLFGKELHKVE 284 KHNEAYTWTNPTCCVHNVIIGKLWIEQYGTVEILNHRTGHKCVLHFKPCGLFGKELHKVE Sbjct: 200 KHNEAYTWTNPTCCVHNVIIGKLWIEQYGTVEILNHRTGHKCVLHFKPCGLFGKELHKVE 259 Query: 285 GHIQDKNKKKLFMIYGKWTECLWGIDPVSYESFKKQERRGDHLRKAKLDEDSGKADSDVA 344 GHIQDKNKKKLFMIYGKWTECLWGIDPVSYESFKKQERRGDHLRKAKLDEDSGKADSDVA Sbjct: 260 GHIQDKNKKKLFMIYGKWTECLWGIDPVSYESFKKQERRGDHLRKAKLDEDSGKADSDVA 319 Query: 345 DDVPVAQETVQVIPGSKLLWRINTRPPNSAQMYNFTSFTVSLNELETGMEKTLPPTDCRL 404 DDVPVAQETVQVIPGSKLLWRINTRPPNSAQMYNFTSFTVSLNELETGMEKTLPPTDCRL Sbjct: 320 DDVPVAQETVQVIPGSKLLWRINTRPPNSAQMYNFTSFTVSLNELETGMEKTLPPTDCRL 379 Query: 405 RPDIRGMENGNMDLASQEKERLEEKQREARRERAKEEAEWQTRWFYPGNNPYTGTPDWLY 464 RPDIRGMENGNMDLASQEKERLEEKQREARRERAKEEAEWQTRWFYPGNNPYTGTPDWLY Sbjct: 380 RPDIRGMENGNMDLASQEKERLEEKQREARRERAKEEAEWQTRWFYPGNNPYTGTPDWLY 439 Query: 465 AGDYFERNFSDCPDIY 480 AGDYFERNFSDCPDIY Sbjct: 440 AGDYFERNFSDCPDIY 455 >4B2Z-A nrPDB Length = 448 Score = 90.1 bits (222), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 65/236 (27%), Positives = 106/236 (44%), Gaps = 13/236 (5%) Query: 85 GLELSKITMPIAFNEPLSFLQRITEYMEHVYLIHRASCQPQPLERMQSVAAFAVSAVASQ 144 G +L++IT+P E S L+R+T ++ + +A + PL+R V + ++ Sbjct: 61 GCDLTRITLPTFILEKKSMLERVTNQLQFPEFLLQAHSEKDPLKRFLYVMKWYLAGWHIA 120 Query: 145 WERTGKPFNPLLGETY----ELIREDLGFRFISEQVSHHPPISAFHSEGLNHDFLFHGSI 200 + KP NP+LGE + +L + + +ISEQ SHHPP A+ G + Sbjct: 121 PKAVKKPLNPVLGEYFTAYWDLPNKQQAY-YISEQTSHHPPECAYFYMIPESSIRVDGVV 179 Query: 201 YPKLKFWGKSVEAEPRGTITLELLK------HNEAYTWTNPTCCVHNVIIGKLWIEQYGT 254 PK +F G S A G+ L+ L E Y T P V ++ GK+ IE G Sbjct: 180 IPKSRFLGNSSAAMMDGSTVLQFLDIKDGNGKPEKYVLTQPNVYVRGILFGKMRIE-LGD 238 Query: 255 VEILNHRTGHKCVLHFKPCGLFGKELHKVEGHIQDKNKKKLFMIYGKWTECLWGID 310 I+ + + FK G +EG ++D + + I GKW + ++ D Sbjct: 239 HMIIK-SPNFQADIEFKTKGYVFGTYDAIEGTVKDYDGNAYYEISGKWNDVMYLKD 293 >1ZI7-A nrPDB Length = 406 Score = 66.2 bits (160), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 104/388 (26%), Positives = 159/388 (40%), Gaps = 88/388 (22%) Query: 94 PIAFNEPLSFLQRITEYMEHVYLIHRASCQPQPLERMQSVAAFAVSAVASQW----ERTG 149 P F EP SF+ Y EH LI P+ L RM +V + +S + SQ+ E G Sbjct: 25 PELFLEP-SFIND-DNYKEHC-LIDPEVESPE-LARMLAVTKWFISTLKSQYCSRNESLG 80 Query: 150 ---KPFNPLLGETYELIREDLGF------RFISEQVSHHPPISAFHSEGLNHDFLFHGSI 200 KP NP LGE + E+ +SEQVSHHPP++AF + G Sbjct: 81 SEKKPLNPFLGELFVGKWENKEHPEFGETVLLSEQVSHHPPVTAFSIFNDKNKVKLQGYN 140 Query: 201 YPKLKFWGKSVEAEPRGTITLELLKHNEAYTWTNPTCCVHNVIIGKLWIEQYGTVEILNH 260 K F KS+ + L +E+Y T P + +++ ++E G I Sbjct: 141 QIKASF-TKSLMLTVKQFGHTMLDIKDESYLVTPPPLHIEGILVASPFVELEGKSYI-QS 198 Query: 261 RTGHKCVLHFKPCGLFGKE------LHKVEGHIQDKNKKKLFMIYGKWTECLWGIDPVSY 314 TG CV+ F G+ GK+ ++K +DK +K L+ I G+W+ G + Sbjct: 199 STGLLCVIEF--SGVDGKKNSFKARIYKDSKDSKDK-EKALYTISGQWS----GSSKI-I 250 Query: 315 ESFKKQERRGDHLRKAKLDEDSGKADSDVADDVPVAQETVQVIPGSKLLWRINTRPPNSA 374 ++ KK+E R L D+ + IP L N +P Sbjct: 251 KANKKEESR--------LFYDAAR------------------IPAEHL----NVKP---- 276 Query: 375 QMYNFTSFTVSLNELETGMEKTLPPTDCRLRPDIRG-MENGNMDLASQEKERLEEKQREA 433 +E+ P + D+ G ++ G+ +L ++ K LEE QRE Sbjct: 277 ------------------LEEQHPLESRKAWYDVAGAIKLGDFNLIAKTKTELEETQREL 318 Query: 434 RRERAKEEAEWQTRWFYPGNNPYTGTPD 461 R+E + WQ RWF + Y+ TP+ Sbjct: 319 RKEEEAKGISWQRRWF--KDFDYSVTPE 344 >3H6Q-A nrPDB Length = 169 Score = 28.1 bits (61), Expect = 8.4, Method: Compositional matrix adjust. Identities = 17/46 (36%), Positives = 20/46 (43%), Gaps = 2/46 (4%) Query: 339 ADSDVADDVPVAQETVQVIPGSKLLWRINTRPPNSAQMYNFTSFTV 384 A S DVPV E + P SK+ W I P MY T F + Sbjct: 29 ASSKDGKDVPVTAEPLG--PQSKIRWWIARDPQAGDDMYTITEFRI 72 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5ZM8-A nrPDB 802 0.0 1ZI7-A nrPDB 350 8e-97 4B2Z-A nrPDB 330 1e-90 Sequences not found previously or not previously below threshold: 3CCY-A nrPDB 34 0.18 5ZDO-A nrPDB 31 1.5 3JCR-F nrPDB 29 4.2 5OQL-B nrPDB 28 8.0 6ID1-L nrPDB 28 9.9 CONVERGED! >5ZM8-A nrPDB Length = 455 Score = 802 bits (2070), Expect = 0.0, Method: Composition-based stats. Identities = 433/436 (99%), Positives = 433/436 (99%) Query: 45 GKTGERPSQENGIQKHRTSLPAPMFSRSDFSVWTILKKCVGLELSKITMPIAFNEPLSFL 104 G ERPSQENGIQKHRTSLPAPMFSRSDFSVWTILKKCVGLELSKITMPIAFNEPLSFL Sbjct: 20 GPHMERPSQENGIQKHRTSLPAPMFSRSDFSVWTILKKCVGLELSKITMPIAFNEPLSFL 79 Query: 105 QRITEYMEHVYLIHRASCQPQPLERMQSVAAFAVSAVASQWERTGKPFNPLLGETYELIR 164 QRITEYMEHVYLIHRASCQPQPLERMQSVAAFAVSAVASQWERTGKPFNPLLGETYELIR Sbjct: 80 QRITEYMEHVYLIHRASCQPQPLERMQSVAAFAVSAVASQWERTGKPFNPLLGETYELIR 139 Query: 165 EDLGFRFISEQVSHHPPISAFHSEGLNHDFLFHGSIYPKLKFWGKSVEAEPRGTITLELL 224 EDLGFRFISEQVSHHPPISAFHSEGLNHDFLFHGSIYPKLKFWGKSVEAEPRGTITLELL Sbjct: 140 EDLGFRFISEQVSHHPPISAFHSEGLNHDFLFHGSIYPKLKFWGKSVEAEPRGTITLELL 199 Query: 225 KHNEAYTWTNPTCCVHNVIIGKLWIEQYGTVEILNHRTGHKCVLHFKPCGLFGKELHKVE 284 KHNEAYTWTNPTCCVHNVIIGKLWIEQYGTVEILNHRTGHKCVLHFKPCGLFGKELHKVE Sbjct: 200 KHNEAYTWTNPTCCVHNVIIGKLWIEQYGTVEILNHRTGHKCVLHFKPCGLFGKELHKVE 259 Query: 285 GHIQDKNKKKLFMIYGKWTECLWGIDPVSYESFKKQERRGDHLRKAKLDEDSGKADSDVA 344 GHIQDKNKKKLFMIYGKWTECLWGIDPVSYESFKKQERRGDHLRKAKLDEDSGKADSDVA Sbjct: 260 GHIQDKNKKKLFMIYGKWTECLWGIDPVSYESFKKQERRGDHLRKAKLDEDSGKADSDVA 319 Query: 345 DDVPVAQETVQVIPGSKLLWRINTRPPNSAQMYNFTSFTVSLNELETGMEKTLPPTDCRL 404 DDVPVAQETVQVIPGSKLLWRINTRPPNSAQMYNFTSFTVSLNELETGMEKTLPPTDCRL Sbjct: 320 DDVPVAQETVQVIPGSKLLWRINTRPPNSAQMYNFTSFTVSLNELETGMEKTLPPTDCRL 379 Query: 405 RPDIRGMENGNMDLASQEKERLEEKQREARRERAKEEAEWQTRWFYPGNNPYTGTPDWLY 464 RPDIRGMENGNMDLASQEKERLEEKQREARRERAKEEAEWQTRWFYPGNNPYTGTPDWLY Sbjct: 380 RPDIRGMENGNMDLASQEKERLEEKQREARRERAKEEAEWQTRWFYPGNNPYTGTPDWLY 439 Query: 465 AGDYFERNFSDCPDIY 480 AGDYFERNFSDCPDIY Sbjct: 440 AGDYFERNFSDCPDIY 455 >1ZI7-A nrPDB Length = 406 Score = 350 bits (897), Expect = 8e-97, Method: Composition-based stats. Identities = 104/388 (26%), Positives = 159/388 (40%), Gaps = 88/388 (22%) Query: 94 PIAFNEPLSFLQRITEYMEHVYLIHRASCQPQPLERMQSVAAFAVSAVASQW----ERTG 149 P F EP SF+ Y EH LI P+ L RM +V + +S + SQ+ E G Sbjct: 25 PELFLEP-SFIND-DNYKEHC-LIDPEVESPE-LARMLAVTKWFISTLKSQYCSRNESLG 80 Query: 150 ---KPFNPLLGETYELIREDLGF------RFISEQVSHHPPISAFHSEGLNHDFLFHGSI 200 KP NP LGE + E+ +SEQVSHHPP++AF + G Sbjct: 81 SEKKPLNPFLGELFVGKWENKEHPEFGETVLLSEQVSHHPPVTAFSIFNDKNKVKLQGYN 140 Query: 201 YPKLKFWGKSVEAEPRGTITLELLKHNEAYTWTNPTCCVHNVIIGKLWIEQYGTVEILNH 260 K F KS+ + L +E+Y T P + +++ ++E G I Sbjct: 141 QIKASF-TKSLMLTVKQFGHTMLDIKDESYLVTPPPLHIEGILVASPFVELEGKSYI-QS 198 Query: 261 RTGHKCVLHFKPCGLFGKE------LHKVEGHIQDKNKKKLFMIYGKWTECLWGIDPVSY 314 TG CV+ F G+ GK+ ++K +DK +K L+ I G+W+ G + Sbjct: 199 STGLLCVIEF--SGVDGKKNSFKARIYKDSKDSKDK-EKALYTISGQWS----GSSKI-I 250 Query: 315 ESFKKQERRGDHLRKAKLDEDSGKADSDVADDVPVAQETVQVIPGSKLLWRINTRPPNSA 374 ++ KK+E R L D+ + IP L N +P Sbjct: 251 KANKKEESR--------LFYDAAR------------------IPAEHL----NVKP---- 276 Query: 375 QMYNFTSFTVSLNELETGMEKTLPPTDCRLRPDIRG-MENGNMDLASQEKERLEEKQREA 433 +E+ P + D+ G ++ G+ +L ++ K LEE QRE Sbjct: 277 ------------------LEEQHPLESRKAWYDVAGAIKLGDFNLIAKTKTELEETQREL 318 Query: 434 RRERAKEEAEWQTRWFYPGNNPYTGTPD 461 R+E + WQ RWF + Y+ TP+ Sbjct: 319 RKEEEAKGISWQRRWF--KDFDYSVTPE 344 >4B2Z-A nrPDB Length = 448 Score = 330 bits (845), Expect = 1e-90, Method: Composition-based stats. Identities = 85/376 (22%), Positives = 146/376 (38%), Gaps = 70/376 (18%) Query: 85 GLELSKITMPIAFNEPLSFLQRITEYMEHVYLIHRASCQPQPLERMQSVAAFAVSAVASQ 144 G +L++IT+P E S L+R+T ++ + +A + PL+R V + ++ Sbjct: 61 GCDLTRITLPTFILEKKSMLERVTNQLQFPEFLLQAHSEKDPLKRFLYVMKWYLAGWHIA 120 Query: 145 WERTGKPFNPLLGETY----ELIREDLGFRFISEQVSHHPPISAFHSEGLNHDFLFHGSI 200 + KP NP+LGE + +L + + +ISEQ SHHPP A+ G + Sbjct: 121 PKAVKKPLNPVLGEYFTAYWDLPNKQQAY-YISEQTSHHPPECAYFYMIPESSIRVDGVV 179 Query: 201 YPKLKFWGKSVEAEPRGTITLELLK------HNEAYTWTNPTCCVHNVIIGKLWIEQYGT 254 PK +F G S A G+ L+ L E Y T P V ++ GK+ IE G Sbjct: 180 IPKSRFLGNSSAAMMDGSTVLQFLDIKDGNGKPEKYVLTQPNVYVRGILFGKMRIEL-GD 238 Query: 255 VEILNHRTGHKCVLHFKPCGLFGKELHKVEGHIQDKNKKKLFMIYGKWTECLWGIDPVSY 314 I+ + + FK G +EG ++D + + I GKW + ++ D Sbjct: 239 HMIIK-SPNFQADIEFKTKGYVFGTYDAIEGTVKDYDGNAYYEISGKWNDVMYLKD---- 293 Query: 315 ESFKKQERRGDHLRKAKLDEDSGKADSDVADDVPVAQETVQVIPGSKLLWRINTRPPNSA 374 KQ R K+ D+ K R RP Sbjct: 294 ---LKQPR-----SSPKVFLDTHKESP----------------------LRPKVRP---- 319 Query: 375 QMYNFTSFTVSLNELETGMEKTLPPTDCRLRPDIR-GMENGNMDLASQEKERLEEKQREA 433 + + +L + + N +A++EK ++E+ QR+ Sbjct: 320 ------------------LSEQGEYESRKLWKKVTDALAVRNHPVATEEKFQIEDHQRQL 361 Query: 434 RRERAKEEAEWQTRWF 449 ++R ++ E+ + F Sbjct: 362 AKKRIEDGVEFHPKLF 377 >3CCY-A nrPDB Length = 203 Score = 33.6 bits (75), Expect = 0.18, Method: Composition-based stats. Identities = 21/115 (18%), Positives = 44/115 (38%), Gaps = 12/115 (10%) Query: 66 APMFSRSDFSVWTILKKCVGLELSKITMPIAFNEPLSFLQR-----ITEYMEHVYLIHRA 120 A MF+R +S +I E SK + F+ + L+ + +E + Sbjct: 24 AAMFARQGYSETSIGDIARACECSKSRLYHYFDSKEAVLRDMLTTHVDSLLERCRQVLYG 83 Query: 121 SCQPQPLERMQSVAAFAVSAVASQWERTGKPFNPLLGETYELIREDLGFRFISEQ 175 S +P+ R + + A+ +R + ++ + + ED I++Q Sbjct: 84 SNEPK--TRFLQIVKLFLEIYATSRDR-----HVVMLTCLDALPEDQRKALIAKQ 131 >5ZDO-A nrPDB Length = 548 Score = 30.6 bits (67), Expect = 1.5, Method: Composition-based stats. Identities = 15/46 (32%), Positives = 18/46 (39%), Gaps = 6/46 (13%) Query: 425 RLEEKQREARRERAKEEAEWQTRWFYPGNNPYTGTPDWLYAGDYFE 470 R ++ E +E E RW G P LYA DYFE Sbjct: 62 RFDDTNPETEKEEYARAIEEDVRWL--GFRPTRV----LYASDYFE 101 >3JCR-F nrPDB Length = 820 Score = 29.0 bits (63), Expect = 4.2, Method: Composition-based stats. Identities = 14/49 (28%), Positives = 24/49 (48%), Gaps = 3/49 (6%) Query: 421 QEKERLEEKQREARRERAKEEAEWQTRWFYPGNNPYTGTPDW-LYAGDY 468 +EKE+ E + R+ R++ AK+ W R + DW ++ DY Sbjct: 331 EEKEQEEARLRKLRKKEAKQ--RWDDRHWSQKKLDEMTDRDWRIFREDY 377 >5OQL-B nrPDB Length = 907 Score = 28.3 bits (61), Expect = 8.0, Method: Composition-based stats. Identities = 27/128 (21%), Positives = 44/128 (34%), Gaps = 19/128 (14%) Query: 320 QERRGDHLRKAKLDEDSGKADSDVADDVPVAQETVQVIPGSKLLWRINTRPPNSAQMYNF 379 E R H+ K D+D + DD+P E+ + L R Sbjct: 158 DEARLTHMGKPLFDDDEAPKEDFSEDDLPSGDESDTTRAERRALKR-------------- 203 Query: 380 TSFTVSLNELETGME-KTLPPTDCRLRPDIRGMENGNMDLASQEKERLEEKQREARRERA 438 L E G+E + P + R +I + E++ ++E+ E R E Sbjct: 204 ----QRLAEAMEGLEDEAGQPERKKTRKEIYEEIIAKSKMYKAERQAIKEENNELRMEVD 259 Query: 439 KEEAEWQT 446 +E E Q Sbjct: 260 QELPEVQQ 267 >6ID1-L nrPDB Length = 802 Score = 27.9 bits (60), Expect = 9.9, Method: Composition-based stats. Identities = 40/177 (22%), Positives = 68/177 (38%), Gaps = 16/177 (9%) Query: 286 HIQDKNKKKLFMIYGK--WTECLWGIDPVSYESFKKQ--ERRGDHLRKAKLDEDSGKADS 341 + +D+ K M YGK W+ + S + K + E ++K + + + Sbjct: 13 NTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKKTEWSREEEEKLL 72 Query: 342 DVADDVPVAQETVQVIPG---SKLLWRINTRPPNSAQMYNFTSFTVSLNELETGMEKTLP 398 +A +P T+ I G ++ L +AQ N T +L+ G E Sbjct: 73 HLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDPRKLKPG-EIDPN 131 Query: 399 PTDCRLRPDIRGMENGNMDLASQEKERLEEKQ--------REARRERAKEEAEWQTR 447 P RPD M+ +++ S+ + RL Q RE + E A+ A Q R Sbjct: 132 PETKPARPDPIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKR 188 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.305 0.121 0.335 Lambda K H 0.267 0.0369 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 18,446,580 Number of Sequences: 36641 Number of extensions: 716767 Number of successful extensions: 1912 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 1863 Number of HSP's gapped (non-prelim): 43 length of query: 480 length of database: 10,336,785 effective HSP length: 101 effective length of query: 379 effective length of database: 6,636,044 effective search space: 2515060676 effective search space used: 2515060676 T: 11 A: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.1 bits) S2: 61 (28.3 bits)