BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9FNG3 (206 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2Q3S-A nrPDB 418 e-118 5V5F-A nrPDB 291 2e-79 6B1T-M nrPDB 32 0.26 5TX1-N nrPDB 30 0.96 4AR2-A nrPDB 30 1.0 5B8F-A nrPDB 29 1.2 3CKC-A nrPDB 29 1.4 4MC5-A nrPDB 28 3.8 4K3X-A nrPDB 27 4.5 4W8Y-A nrPDB 27 7.1 6REQ-B nrPDB 27 9.1 >2Q3S-A nrPDB Length = 206 Score = 418 bits (1075), Expect = e-118, Method: Compositional matrix adjust. Identities = 205/205 (100%), Positives = 205/205 (100%) Query: 2 ASFDGPKFKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVYWDVGFPE 61 ASFDGPKFKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVYWDVGFPE Sbjct: 2 ASFDGPKFKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVYWDVGFPE 61 Query: 62 TETKTKTSGWSLSSVKLSTRNLCLFLRLPKPFHDNLKDLYRFFASKFVTFVGVQIEEDLD 121 TETKTKTSGWSLSSVKLSTRNLCLFLRLPKPFHDNLKDLYRFFASKFVTFVGVQIEEDLD Sbjct: 62 TETKTKTSGWSLSSVKLSTRNLCLFLRLPKPFHDNLKDLYRFFASKFVTFVGVQIEEDLD 121 Query: 122 LLRENHGLVIRNAINVGKLAAEARGTLVLEFLGTRELAHRVLWSDLGQLDSIEAKWEKAG 181 LLRENHGLVIRNAINVGKLAAEARGTLVLEFLGTRELAHRVLWSDLGQLDSIEAKWEKAG Sbjct: 122 LLRENHGLVIRNAINVGKLAAEARGTLVLEFLGTRELAHRVLWSDLGQLDSIEAKWEKAG 181 Query: 182 PEEQLEAAAIEGWLIVNVWDQLSDE 206 PEEQLEAAAIEGWLIVNVWDQLSDE Sbjct: 182 PEEQLEAAAIEGWLIVNVWDQLSDE 206 >5V5F-A nrPDB Length = 200 Score = 291 bits (745), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 138/203 (67%), Positives = 169/203 (83%), Gaps = 5/203 (2%) Query: 1 MASFDGPKFKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVYWDVGFP 60 MASFDG F M D S+VQTK IDV S+TDISPYLS I EDS+ NGNR+++FDVYWDV Sbjct: 1 MASFDGQGFMMVDNSWVQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFDVYWDV--- 57 Query: 61 ETETKTKTSGWSLSSVKLSTRNLCLFLRLPKPFHDNLKDLYRFFASKFVTFVGVQIEEDL 120 ++ + S W L SVK ST+N CLFLRLP PF DNLKDLYRFFASKFVTFVGVQI+EDL Sbjct: 58 --KSVSTKSEWRLCSVKFSTKNFCLFLRLPNPFCDNLKDLYRFFASKFVTFVGVQIQEDL 115 Query: 121 DLLRENHGLVIRNAINVGKLAAEARGTLVLEFLGTRELAHRVLWSDLGQLDSIEAKWEKA 180 LL+ENHG+VIR+++ +GKLAA+ARGT ++EFLGTRELAH++LW D+ +LDSI++KW++A Sbjct: 116 ALLKENHGIVIRSSLEIGKLAAKARGTPIVEFLGTRELAHKILWYDMSRLDSIQSKWDEA 175 Query: 181 GPEEQLEAAAIEGWLIVNVWDQL 203 ++LEAAAIEGWLI NV+DQL Sbjct: 176 SSNDRLEAAAIEGWLIFNVYDQL 198 >6B1T-M nrPDB Length = 571 Score = 31.6 bits (70), Expect = 0.26, Method: Compositional matrix adjust. Identities = 14/46 (30%), Positives = 28/46 (60%) Query: 8 KFKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDV 53 +F + +G+Y +T TID+ ++ + YL + R++ +L + V FD Sbjct: 167 EFTLPEGNYSETMTIDLMNNAIVEHYLKVGRQNGVLESDIGVKFDT 212 >5TX1-N nrPDB Length = 497 Score = 29.6 bits (65), Expect = 0.96, Method: Compositional matrix adjust. Identities = 13/45 (28%), Positives = 28/45 (62%) Query: 8 KFKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFD 52 +F + +G++ +T TID+ ++ + YL + R++ +L + V FD Sbjct: 138 EFTLPEGNFSETMTIDLMNNAILENYLQVGRQNGVLESDIGVKFD 182 >4AR2-A nrPDB Length = 509 Score = 29.6 bits (65), Expect = 1.0, Method: Compositional matrix adjust. Identities = 19/61 (31%), Positives = 33/61 (54%), Gaps = 8/61 (13%) Query: 8 KFKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVY-----WDVGFPET 62 +F + +G++ T TID+ ++ I YL + R++ +L + V FD WD PET Sbjct: 176 EFILPEGNFSATMTIDLMNNAIIDNYLEIGRQNGVLESDIGVKFDTRNFRLGWD---PET 232 Query: 63 E 63 + Sbjct: 233 K 233 >5B8F-A nrPDB Length = 164 Score = 29.3 bits (64), Expect = 1.2, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 28/54 (51%) Query: 99 DLYRFFASKFVTFVGVQIEEDLDLLRENHGLVIRNAINVGKLAAEARGTLVLEF 152 D++RF F+T GV+I L+ + G V+ +A++ L A A G + F Sbjct: 15 DVHRFGEGDFITLGGVRIPHKHGLVAHSDGDVLLHALSDALLGAAALGDIGKHF 68 >3CKC-A nrPDB Length = 527 Score = 29.3 bits (64), Expect = 1.4, Method: Compositional matrix adjust. Identities = 27/102 (26%), Positives = 40/102 (39%), Gaps = 19/102 (18%) Query: 57 VGFPETETKTKTSGWSLSSVKLSTRNLCLFLR--LPKPFHDNLKDLYRFFASKFVTFVGV 114 G P T T+GWS C+F R + + F NL+D+ A + G+ Sbjct: 285 AGMPRMGT---TNGWS-----------CIFARAAMVQKFFSNLEDVPMLPADVEIPTKGL 330 Query: 115 QIEEDLDLLRENHGLVIRNAINVGKLAAEARGTLVLEFLGTR 156 +E +D HG+ + I K A + R L G R Sbjct: 331 DTDEQIDAFDAEHGIRTEDMI---KAAGDDRALLYSGVGGGR 369 >4MC5-A nrPDB Length = 510 Score = 27.7 bits (60), Expect = 3.8, Method: Compositional matrix adjust. Identities = 19/57 (33%), Positives = 28/57 (49%), Gaps = 3/57 (5%) Query: 14 GSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVYWDVGFPETETKTKTSG 70 GS + TI +G T+ P + I E SILNG+ I + +W V P ++G Sbjct: 194 GSQTGSTTITIGEETNTYPLV--ISESSILNGHSDRI-NYFWGVVNPNQNFSIVSTG 247 >4K3X-A nrPDB Length = 329 Score = 27.3 bits (59), Expect = 4.5, Method: Compositional matrix adjust. Identities = 19/57 (33%), Positives = 28/57 (49%), Gaps = 3/57 (5%) Query: 14 GSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVYWDVGFPETETKTKTSG 70 GS + TI +G T+ P + I E SILNG+ I + +W V P ++G Sbjct: 194 GSQTGSTTITIGEETNTYPLV--ISESSILNGHSDRI-NYFWGVVNPNQNFSIVSTG 247 >4W8Y-A nrPDB Length = 888 Score = 26.6 bits (57), Expect = 7.1, Method: Compositional matrix adjust. Identities = 26/86 (30%), Positives = 43/86 (50%), Gaps = 9/86 (10%) Query: 109 VTFVGVQIEEDLDLLRENHGLVIRNAINVGKLAAEARGTLVLEFLGTRELAHRVLWSDLG 168 +T+V + +ED+ + EN GL + K A + + VLE + L ++L S LG Sbjct: 387 ITWVSLSEKEDIYQVTENAGLSDEDVKKWLKFAEKKENSRVLERIAIYPLLVKILDS-LG 445 Query: 169 QLDSIEAKWEKAGPEEQLEAAAIEGW 194 + E ++EK+ EQL +GW Sbjct: 446 ERKVTEERFEKS---EQL-----KGW 463 >6REQ-B nrPDB Length = 637 Score = 26.6 bits (57), Expect = 9.1, Method: Compositional matrix adjust. Identities = 17/58 (29%), Positives = 32/58 (55%), Gaps = 3/58 (5%) Query: 117 EEDLDL-LRENHGLVIRNAINVGKLAAEARGTLVLEFLGTRELAHRVLWSDLGQLDSI 173 E+D L + N G+V+ +N+G++ A G+ +E L TR LA W + +++ + Sbjct: 370 EDDFPLRIARNTGIVLAEEVNIGRVNDPAGGSYYVESL-TRSLAD-AAWKEFQEVEKL 425 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2Q3S-A nrPDB 373 e-104 5V5F-A nrPDB 358 1e-99 Sequences not found previously or not previously below threshold: 6B1T-M nrPDB 29 1.7 5B8F-A nrPDB 29 1.7 4AR2-A nrPDB 28 2.4 3QS3-A nrPDB 27 4.5 5TX1-N nrPDB 27 6.2 5O57-A nrPDB 27 6.8 6REQ-B nrPDB 27 7.2 3RH9-A nrPDB 26 9.9 CONVERGED! >2Q3S-A nrPDB Length = 206 Score = 373 bits (958), Expect = e-104, Method: Composition-based stats. Identities = 205/205 (100%), Positives = 205/205 (100%) Query: 2 ASFDGPKFKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVYWDVGFPE 61 ASFDGPKFKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVYWDVGFPE Sbjct: 2 ASFDGPKFKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVYWDVGFPE 61 Query: 62 TETKTKTSGWSLSSVKLSTRNLCLFLRLPKPFHDNLKDLYRFFASKFVTFVGVQIEEDLD 121 TETKTKTSGWSLSSVKLSTRNLCLFLRLPKPFHDNLKDLYRFFASKFVTFVGVQIEEDLD Sbjct: 62 TETKTKTSGWSLSSVKLSTRNLCLFLRLPKPFHDNLKDLYRFFASKFVTFVGVQIEEDLD 121 Query: 122 LLRENHGLVIRNAINVGKLAAEARGTLVLEFLGTRELAHRVLWSDLGQLDSIEAKWEKAG 181 LLRENHGLVIRNAINVGKLAAEARGTLVLEFLGTRELAHRVLWSDLGQLDSIEAKWEKAG Sbjct: 122 LLRENHGLVIRNAINVGKLAAEARGTLVLEFLGTRELAHRVLWSDLGQLDSIEAKWEKAG 181 Query: 182 PEEQLEAAAIEGWLIVNVWDQLSDE 206 PEEQLEAAAIEGWLIVNVWDQLSDE Sbjct: 182 PEEQLEAAAIEGWLIVNVWDQLSDE 206 >5V5F-A nrPDB Length = 200 Score = 358 bits (918), Expect = 1e-99, Method: Composition-based stats. Identities = 138/203 (67%), Positives = 169/203 (83%), Gaps = 5/203 (2%) Query: 1 MASFDGPKFKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVYWDVGFP 60 MASFDG F M D S+VQTK IDV S+TDISPYLS I EDS+ NGNR+++FDVYWDV Sbjct: 1 MASFDGQGFMMVDNSWVQTKAIDVESTTDISPYLSKILEDSVWNGNRSIVFDVYWDV--- 57 Query: 61 ETETKTKTSGWSLSSVKLSTRNLCLFLRLPKPFHDNLKDLYRFFASKFVTFVGVQIEEDL 120 ++ + S W L SVK ST+N CLFLRLP PF DNLKDLYRFFASKFVTFVGVQI+EDL Sbjct: 58 --KSVSTKSEWRLCSVKFSTKNFCLFLRLPNPFCDNLKDLYRFFASKFVTFVGVQIQEDL 115 Query: 121 DLLRENHGLVIRNAINVGKLAAEARGTLVLEFLGTRELAHRVLWSDLGQLDSIEAKWEKA 180 LL+ENHG+VIR+++ +GKLAA+ARGT ++EFLGTRELAH++LW D+ +LDSI++KW++A Sbjct: 116 ALLKENHGIVIRSSLEIGKLAAKARGTPIVEFLGTRELAHKILWYDMSRLDSIQSKWDEA 175 Query: 181 GPEEQLEAAAIEGWLIVNVWDQL 203 ++LEAAAIEGWLI NV+DQL Sbjct: 176 SSNDRLEAAAIEGWLIFNVYDQL 198 >6B1T-M nrPDB Length = 571 Score = 28.8 bits (63), Expect = 1.7, Method: Composition-based stats. Identities = 14/44 (31%), Positives = 27/44 (61%) Query: 9 FKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFD 52 F + +G+Y +T TID+ ++ + YL + R++ +L + V FD Sbjct: 168 FTLPEGNYSETMTIDLMNNAIVEHYLKVGRQNGVLESDIGVKFD 211 >5B8F-A nrPDB Length = 164 Score = 28.8 bits (63), Expect = 1.7, Method: Composition-based stats. Identities = 16/48 (33%), Positives = 26/48 (54%) Query: 99 DLYRFFASKFVTFVGVQIEEDLDLLRENHGLVIRNAINVGKLAAEARG 146 D++RF F+T GV+I L+ + G V+ +A++ L A A G Sbjct: 15 DVHRFGEGDFITLGGVRIPHKHGLVAHSDGDVLLHALSDALLGAAALG 62 >4AR2-A nrPDB Length = 509 Score = 28.4 bits (62), Expect = 2.4, Method: Composition-based stats. Identities = 19/60 (31%), Positives = 32/60 (53%), Gaps = 8/60 (13%) Query: 9 FKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVY-----WDVGFPETE 63 F + +G++ T TID+ ++ I YL + R++ +L + V FD WD PET+ Sbjct: 177 FILPEGNFSATMTIDLMNNAIIDNYLEIGRQNGVLESDIGVKFDTRNFRLGWD---PETK 233 >3QS3-A nrPDB Length = 191 Score = 27.3 bits (59), Expect = 4.5, Method: Composition-based stats. Identities = 15/68 (22%), Positives = 25/68 (36%), Gaps = 10/68 (14%) Query: 5 DGPKFKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFDVYWDVGFPETET 64 DG F + G+ T + TD S + I +G+ +V FD W + Sbjct: 128 DGFTFSIISGTTNGTTAV-----TDYS-----TLPEGIWSGDVSVQFDATWTSSDNKLRA 177 Query: 65 KTKTSGWS 72 + W+ Sbjct: 178 AVSKTTWA 185 >5TX1-N nrPDB Length = 497 Score = 26.9 bits (58), Expect = 6.2, Method: Composition-based stats. Identities = 13/44 (29%), Positives = 27/44 (61%) Query: 9 FKMTDGSYVQTKTIDVGSSTDISPYLSLIREDSILNGNRAVIFD 52 F + +G++ +T TID+ ++ + YL + R++ +L + V FD Sbjct: 139 FTLPEGNFSETMTIDLMNNAILENYLQVGRQNGVLESDIGVKFD 182 >5O57-A nrPDB Length = 95 Score = 26.9 bits (58), Expect = 6.8, Method: Composition-based stats. Identities = 12/37 (32%), Positives = 15/37 (40%) Query: 66 TKTSGWSLSSVKLSTRNLCLFLRLPKPFHDNLKDLYR 102 + LS +TR CL R KPF + L R Sbjct: 18 ARKGSQCLSDTDCNTRKFCLQPRDEKPFCATCRGLRR 54 >6REQ-B nrPDB Length = 637 Score = 26.9 bits (58), Expect = 7.2, Method: Composition-based stats. Identities = 17/58 (29%), Positives = 32/58 (55%), Gaps = 3/58 (5%) Query: 117 EEDLDL-LRENHGLVIRNAINVGKLAAEARGTLVLEFLGTRELAHRVLWSDLGQLDSI 173 E+D L + N G+V+ +N+G++ A G+ +E L TR LA W + +++ + Sbjct: 370 EDDFPLRIARNTGIVLAEEVNIGRVNDPAGGSYYVESL-TRSLADAA-WKEFQEVEKL 425 >3RH9-A nrPDB Length = 506 Score = 26.1 bits (56), Expect = 9.9, Method: Composition-based stats. Identities = 13/48 (27%), Positives = 25/48 (52%) Query: 116 IEEDLDLLRENHGLVIRNAINVGKLAAEARGTLVLEFLGTRELAHRVL 163 + + LDL LV+ A +GK+ E + +L F G+ E+ +++ Sbjct: 196 VMDKLDLPDGMVNLVMGKASVIGKVLCEHKDVPMLSFTGSTEVGRKLI 243 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.317 0.141 0.418 Lambda K H 0.267 0.0432 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,539,984 Number of Sequences: 36641 Number of extensions: 338940 Number of successful extensions: 753 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 16 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 735 Number of HSP's gapped (non-prelim): 24 length of query: 206 length of database: 10,336,785 effective HSP length: 92 effective length of query: 114 effective length of database: 6,965,813 effective search space: 794102682 effective search space used: 794102682 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 57 (26.5 bits)