BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= Q9ERE7
         (224 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

2KGL-A nrPDB                                                          397   e-111
2VUU-A nrPDB                                                           29   1.3  
4XD7-H nrPDB                                                           29   1.8  
4Z25-A nrPDB                                                           28   3.2  
4OHS-A nrPDB                                                           28   4.1  
2ID4-A nrPDB                                                           27   7.5  

>2KGL-A nrPDB
          Length = 195

 Score =  397 bits (1021), Expect = e-111,   Method: Compositional matrix adjust.
 Identities = 195/195 (100%), Positives = 195/195 (100%)

Query: 30  ADTPGEATPPPRKKKDIRDYNDADMARLLEQWEKDDDIEEGDLPEHKRPSAPIDFSKLDP 89
           ADTPGEATPPPRKKKDIRDYNDADMARLLEQWEKDDDIEEGDLPEHKRPSAPIDFSKLDP
Sbjct: 1   ADTPGEATPPPRKKKDIRDYNDADMARLLEQWEKDDDIEEGDLPEHKRPSAPIDFSKLDP 60

Query: 90  GKPESILKMTKKGKTLMMFVTVSGNPTEKETEEITSLWQGSLFNANYDVQRFIVGSDRAI 149
           GKPESILKMTKKGKTLMMFVTVSGNPTEKETEEITSLWQGSLFNANYDVQRFIVGSDRAI
Sbjct: 61  GKPESILKMTKKGKTLMMFVTVSGNPTEKETEEITSLWQGSLFNANYDVQRFIVGSDRAI 120

Query: 150 FMLRDGSYAWEIKDFLVSQDRCAEVTLEGQMYPGKGGGSKEKNKTKPEKAKKKEGDPKPR 209
           FMLRDGSYAWEIKDFLVSQDRCAEVTLEGQMYPGKGGGSKEKNKTKPEKAKKKEGDPKPR
Sbjct: 121 FMLRDGSYAWEIKDFLVSQDRCAEVTLEGQMYPGKGGGSKEKNKTKPEKAKKKEGDPKPR 180

Query: 210 ASKEDNRAGSRREDL 224
           ASKEDNRAGSRREDL
Sbjct: 181 ASKEDNRAGSRREDL 195


>2VUU-A nrPDB
          Length = 352

 Score = 29.3 bits (64), Expect = 1.3,   Method: Compositional matrix adjust.
 Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 1/57 (1%)

Query: 74  EHKRPSAPI-DFSKLDPGKPESILKMTKKGKTLMMFVTVSGNPTEKETEEITSLWQG 129
           EHK P  P+ +FS+   G P+ +     KG    M     G  +++ T+E   LW G
Sbjct: 271 EHKAPYFPLPEFSRPAAGSPKGLGPANGKGAGAGMMQGPGGVISQRVTDEARKLWSG 327


>4XD7-H nrPDB
          Length = 133

 Score = 28.9 bits (63), Expect = 1.8,   Method: Compositional matrix adjust.
 Identities = 24/81 (29%), Positives = 36/81 (44%), Gaps = 11/81 (13%)

Query: 68  EEGDLPEHKRPSAPIDFSKLDPGKPESILKMTKKGKTLMMFVTVSGNPTEKETEEITSLW 127
           E G LP H    AP++ S           ++ K GKT   ++ VSG   E   +++T L 
Sbjct: 31  ELGILPGHIPLVAPLEISAA---------RLKKGGKT--QYIAVSGGFLEVRPDKVTILA 79

Query: 128 QGSLFNANYDVQRFIVGSDRA 148
           Q +    + DV R     +RA
Sbjct: 80  QAAERAEDIDVLRAKAAKERA 100


>4Z25-A nrPDB
          Length = 652

 Score = 28.1 bits (61), Expect = 3.2,   Method: Compositional matrix adjust.
 Identities = 21/62 (33%), Positives = 29/62 (46%), Gaps = 1/62 (1%)

Query: 80  APIDFSKLDPGKPESILKMTKKGKTLMMFVTVSGNPTEKETEEITSLWQGSLFNANYDVQ 139
           A + F +LDP KP +   +T +   LMM   V G P E++     S    +LF    D  
Sbjct: 376 AELGFHRLDPNKPANANTVTYRKYQLMMTAGV-GIPAEQQYLSGLSPSSNNLFTLIADDI 434

Query: 140 RF 141
           RF
Sbjct: 435 RF 436


>4OHS-A nrPDB
          Length = 239

 Score = 27.7 bits (60), Expect = 4.1,   Method: Compositional matrix adjust.
 Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 7/52 (13%)

Query: 165 LVSQDRCAEVTLEGQM------YPGKGGGSKEKNKTKPEKAKKKEGDPKPRA 210
           LV++D C ++T+EG +        G+G G K    T+ EK +  EG P P A
Sbjct: 13  LVTEDMCIKMTMEGTINGHHFKCVGEGEG-KPFEGTQVEKIRITEGGPLPFA 63


>2ID4-A nrPDB
          Length = 503

 Score = 26.9 bits (58), Expect = 7.5,   Method: Compositional matrix adjust.
 Identities = 22/84 (26%), Positives = 42/84 (50%), Gaps = 12/84 (14%)

Query: 62  EKDDDIEEGDLPEHKRPSAPIDFSKLDPGKPESILKMTKKGKTL---------MMFVTVS 112
           EK+ D +  D    K+ S    F K+D  K   +++M+K  + +          ++V+ S
Sbjct: 309 EKNADGDWRDSAMGKKYSHRYGFGKIDAHK---LIEMSKTWENVNAQTWFYLPTLYVSQS 365

Query: 113 GNPTEKETEEITSLWQGSLFNANY 136
            N TE+  E + ++ + SL +AN+
Sbjct: 366 TNSTEETLESVITISEKSLQDANF 389


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

2KGL-A nrPDB                                                          395   e-111

Sequences not found previously or not previously below threshold:

2VUU-A nrPDB                                                           33   0.11 
6G4S-u nrPDB                                                           30   0.57 
4Z25-A nrPDB                                                           30   0.83 
4XD7-H nrPDB                                                           30   0.98 
5UQL-A nrPDB                                                           29   1.2  
4OHS-A nrPDB                                                           29   1.4  


CONVERGED!
>2KGL-A nrPDB
          Length = 195

 Score =  395 bits (1015), Expect = e-111,   Method: Composition-based stats.
 Identities = 195/195 (100%), Positives = 195/195 (100%)

Query: 30  ADTPGEATPPPRKKKDIRDYNDADMARLLEQWEKDDDIEEGDLPEHKRPSAPIDFSKLDP 89
           ADTPGEATPPPRKKKDIRDYNDADMARLLEQWEKDDDIEEGDLPEHKRPSAPIDFSKLDP
Sbjct: 1   ADTPGEATPPPRKKKDIRDYNDADMARLLEQWEKDDDIEEGDLPEHKRPSAPIDFSKLDP 60

Query: 90  GKPESILKMTKKGKTLMMFVTVSGNPTEKETEEITSLWQGSLFNANYDVQRFIVGSDRAI 149
           GKPESILKMTKKGKTLMMFVTVSGNPTEKETEEITSLWQGSLFNANYDVQRFIVGSDRAI
Sbjct: 61  GKPESILKMTKKGKTLMMFVTVSGNPTEKETEEITSLWQGSLFNANYDVQRFIVGSDRAI 120

Query: 150 FMLRDGSYAWEIKDFLVSQDRCAEVTLEGQMYPGKGGGSKEKNKTKPEKAKKKEGDPKPR 209
           FMLRDGSYAWEIKDFLVSQDRCAEVTLEGQMYPGKGGGSKEKNKTKPEKAKKKEGDPKPR
Sbjct: 121 FMLRDGSYAWEIKDFLVSQDRCAEVTLEGQMYPGKGGGSKEKNKTKPEKAKKKEGDPKPR 180

Query: 210 ASKEDNRAGSRREDL 224
           ASKEDNRAGSRREDL
Sbjct: 181 ASKEDNRAGSRREDL 195


>2VUU-A nrPDB
          Length = 352

 Score = 33.1 bits (74), Expect = 0.11,   Method: Composition-based stats.
 Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 1/57 (1%)

Query: 74  EHKRPSAPI-DFSKLDPGKPESILKMTKKGKTLMMFVTVSGNPTEKETEEITSLWQG 129
           EHK P  P+ +FS+   G P+ +     KG    M     G  +++ T+E   LW G
Sbjct: 271 EHKAPYFPLPEFSRPAAGSPKGLGPANGKGAGAGMMQGPGGVISQRVTDEARKLWSG 327


>6G4S-u nrPDB
          Length = 804

 Score = 30.4 bits (67), Expect = 0.57,   Method: Composition-based stats.
 Identities = 21/65 (32%), Positives = 29/65 (44%), Gaps = 12/65 (18%)

Query: 31  DTPGEATPPPRKKKDIRDYNDADMARLLEQWEKDDDIEEGDLPEHKRPSAPIDFSKLDPG 90
           D PG+  P P   + I+   D DMA  +   +  DD+EEG               K DPG
Sbjct: 303 DAPGD--PFPLNPRGIKPQKDPDMAMEICATDAVDDMEEGLKV----------LMKADPG 350

Query: 91  KPESI 95
           + ES+
Sbjct: 351 RQESL 355


>4Z25-A nrPDB
          Length = 652

 Score = 30.0 bits (66), Expect = 0.83,   Method: Composition-based stats.
 Identities = 21/62 (33%), Positives = 29/62 (46%), Gaps = 1/62 (1%)

Query: 80  APIDFSKLDPGKPESILKMTKKGKTLMMFVTVSGNPTEKETEEITSLWQGSLFNANYDVQ 139
           A + F +LDP KP +   +T +   LMM   V G P E++     S    +LF    D  
Sbjct: 376 AELGFHRLDPNKPANANTVTYRKYQLMMTAGV-GIPAEQQYLSGLSPSSNNLFTLIADDI 434

Query: 140 RF 141
           RF
Sbjct: 435 RF 436


>4XD7-H nrPDB
          Length = 133

 Score = 29.7 bits (65), Expect = 0.98,   Method: Composition-based stats.
 Identities = 24/81 (29%), Positives = 36/81 (44%), Gaps = 11/81 (13%)

Query: 68  EEGDLPEHKRPSAPIDFSKLDPGKPESILKMTKKGKTLMMFVTVSGNPTEKETEEITSLW 127
           E G LP H    AP++ S           ++ K GKT   ++ VSG   E   +++T L 
Sbjct: 31  ELGILPGHIPLVAPLEISAA---------RLKKGGKT--QYIAVSGGFLEVRPDKVTILA 79

Query: 128 QGSLFNANYDVQRFIVGSDRA 148
           Q +    + DV R     +RA
Sbjct: 80  QAAERAEDIDVLRAKAAKERA 100


>5UQL-A nrPDB
          Length = 558

 Score = 29.3 bits (64), Expect = 1.2,   Method: Composition-based stats.
 Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 4/64 (6%)

Query: 79  SAPIDFSKLDPGKPESILKMTKKGKTLMMFVTVSGNPTEKETEEITSLWQGSLFNANYDV 138
           SA  DF  L     E+ ++ T K   L+ F     N ++   +EI SLW     +A Y  
Sbjct: 475 SAYYDFINLQ----ENTIEKTLKASDLIEFKFPENNLSQLTEQEINSLWSFDQASAKYQF 530

Query: 139 QRFI 142
           ++++
Sbjct: 531 EKYV 534


>4OHS-A nrPDB
          Length = 239

 Score = 29.3 bits (64), Expect = 1.4,   Method: Composition-based stats.
 Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 7/52 (13%)

Query: 165 LVSQDRCAEVTLEGQMYP------GKGGGSKEKNKTKPEKAKKKEGDPKPRA 210
           LV++D C ++T+EG +        G+G G K    T+ EK +  EG P P A
Sbjct: 13  LVTEDMCIKMTMEGTINGHHFKCVGEGEG-KPFEGTQVEKIRITEGGPLPFA 63


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.313    0.133    0.392 

Lambda     K      H
   0.267   0.0415    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,016,583
Number of Sequences: 36641
Number of extensions: 429072
Number of successful extensions: 804
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 780
Number of HSP's gapped (non-prelim): 38
length of query: 224
length of database: 10,336,785
effective HSP length: 93
effective length of query: 131
effective length of database: 6,929,172
effective search space: 907721532
effective search space used: 907721532
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 57 (26.5 bits)