BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9D1L9 (91 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6B9X-E nrPDB 184 5e-48 3MS6-A nrPDB 184 8e-48 5NF4-A nrPDB 27 2.5 2GT3-A nrPDB 25 6.7 1JC5-A nrPDB 25 8.3 6IYY-A nrPDB 25 8.8 >6B9X-E nrPDB Length = 173 Score = 184 bits (468), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 90/91 (98%), Positives = 91/91 (100%) Query: 1 MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAARLTSDPT 60 MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAA+LTSDPT Sbjct: 83 MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAAKLTSDPT 142 Query: 61 DIPVVCLESDNGNIMIQKHDGITVAVHKMAS 91 DIPVVCLESDNGNIMIQKHDGITVAVHKMAS Sbjct: 143 DIPVVCLESDNGNIMIQKHDGITVAVHKMAS 173 >3MS6-A nrPDB Length = 99 Score = 184 bits (467), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 90/91 (98%), Positives = 91/91 (100%) Query: 1 MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAARLTSDPT 60 MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAA+LTSDPT Sbjct: 1 MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAAKLTSDPT 60 Query: 61 DIPVVCLESDNGNIMIQKHDGITVAVHKMAS 91 DIPVVCLESDNGNIMIQKHDGITVAVHKMAS Sbjct: 61 DIPVVCLESDNGNIMIQKHDGITVAVHKMAS 91 >5NF4-A nrPDB Length = 451 Score = 26.6 bits (57), Expect = 2.5, Method: Composition-based stats. Identities = 23/68 (33%), Positives = 31/68 (45%), Gaps = 4/68 (5%) Query: 19 VGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAARLTSDPTDIPVVCLESDNGNIMIQK 78 VGV D QG N+ T+ + V+ ++A+ A S PTD V E NG K Sbjct: 361 VGV---DEQG-NVTITNTMQNCDPHVVRLVAKNGAYFGSQPTDTSVEFTELANGASQTFK 416 Query: 79 HDGITVAV 86 + VAV Sbjct: 417 VNKDAVAV 424 >2GT3-A nrPDB Length = 212 Score = 25.0 bits (53), Expect = 6.7, Method: Compositional matrix adjust. Identities = 11/30 (36%), Positives = 17/30 (56%) Query: 60 TDIPVVCLESDNGNIMIQKHDGITVAVHKM 89 T +PV L + NG+ M DG+ +A+ M Sbjct: 21 TPMPVATLHAVNGHSMTNVPDGMEIAIFAM 50 >1JC5-A nrPDB Length = 148 Score = 25.0 bits (53), Expect = 8.3, Method: Compositional matrix adjust. Identities = 15/50 (30%), Positives = 26/50 (52%), Gaps = 3/50 (6%) Query: 43 GVISVLAQQAARLTSDPTDIPVVCLESDNGNI--MIQKHDGITVAVHKMA 90 GV+ ++ AA+LT T + V+ +D + + KH+G +H MA Sbjct: 45 GVVEIMMAPAAKLTEHMTQVQVMAPLNDESTVAKWLAKHNG-RAGLHHMA 93 >6IYY-A nrPDB Length = 317 Score = 24.6 bits (52), Expect = 8.8, Method: Composition-based stats. Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 6/56 (10%) Query: 8 HLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAARLTSDPTDIP 63 H+ +T NP + VLC +S L GT H G + ++ A P DIP Sbjct: 124 HVFETCYNPKGLCVLCPNSNNSLLAFPGT----HTGHVQLV--DLASTEKPPVDIP 173 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6B9X-E nrPDB 178 5e-46 3MS6-A nrPDB 166 1e-42 Sequences not found previously or not previously below threshold: 5NF4-A nrPDB 27 2.2 2GT3-A nrPDB 27 2.6 1JC5-A nrPDB 26 3.2 6IYY-A nrPDB 25 5.8 CONVERGED! >6B9X-E nrPDB Length = 173 Score = 178 bits (452), Expect = 5e-46, Method: Composition-based stats. Identities = 90/91 (98%), Positives = 91/91 (100%) Query: 1 MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAARLTSDPT 60 MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAA+LTSDPT Sbjct: 83 MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAAKLTSDPT 142 Query: 61 DIPVVCLESDNGNIMIQKHDGITVAVHKMAS 91 DIPVVCLESDNGNIMIQKHDGITVAVHKMAS Sbjct: 143 DIPVVCLESDNGNIMIQKHDGITVAVHKMAS 173 >3MS6-A nrPDB Length = 99 Score = 166 bits (421), Expect = 1e-42, Method: Composition-based stats. Identities = 90/91 (98%), Positives = 91/91 (100%) Query: 1 MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAARLTSDPT 60 MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAA+LTSDPT Sbjct: 1 MEATLEQHLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAAKLTSDPT 60 Query: 61 DIPVVCLESDNGNIMIQKHDGITVAVHKMAS 91 DIPVVCLESDNGNIMIQKHDGITVAVHKMAS Sbjct: 61 DIPVVCLESDNGNIMIQKHDGITVAVHKMAS 91 >5NF4-A nrPDB Length = 451 Score = 26.9 bits (58), Expect = 2.2, Method: Composition-based stats. Identities = 23/68 (33%), Positives = 31/68 (45%), Gaps = 4/68 (5%) Query: 19 VGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAARLTSDPTDIPVVCLESDNGNIMIQK 78 VGV D QG N+ T+ + V+ ++A+ A S PTD V E NG K Sbjct: 361 VGV---DEQG-NVTITNTMQNCDPHVVRLVAKNGAYFGSQPTDTSVEFTELANGASQTFK 416 Query: 79 HDGITVAV 86 + VAV Sbjct: 417 VNKDAVAV 424 >2GT3-A nrPDB Length = 212 Score = 26.5 bits (57), Expect = 2.6, Method: Composition-based stats. Identities = 14/43 (32%), Positives = 20/43 (46%) Query: 48 LAQQAARLTSDPTDIPVVCLESDNGNIMIQKHDGITVAVHKMA 90 L A L T +PV L + NG+ M DG+ +A+ M Sbjct: 9 LVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGMEIAIFAMG 51 >1JC5-A nrPDB Length = 148 Score = 26.1 bits (56), Expect = 3.2, Method: Composition-based stats. Identities = 15/50 (30%), Positives = 26/50 (52%), Gaps = 3/50 (6%) Query: 43 GVISVLAQQAARLTSDPTDIPVVCLESDNGNIM--IQKHDGITVAVHKMA 90 GV+ ++ AA+LT T + V+ +D + + KH+G +H MA Sbjct: 45 GVVEIMMAPAAKLTEHMTQVQVMAPLNDESTVAKWLAKHNG-RAGLHHMA 93 >6IYY-A nrPDB Length = 317 Score = 25.4 bits (54), Expect = 5.8, Method: Composition-based stats. Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 6/56 (10%) Query: 8 HLEDTMKNPSIVGVLCTDSQGLNLGCRGTLSDEHAGVISVLAQQAARLTSDPTDIP 63 H+ +T NP + VLC +S L GT H G + ++ A P DIP Sbjct: 124 HVFETCYNPKGLCVLCPNSNNSLLAFPGT----HTGHVQLV--DLASTEKPPVDIP 173 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.139 0.412 Lambda K H 0.267 0.0400 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,315,118 Number of Sequences: 36641 Number of extensions: 102249 Number of successful extensions: 219 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 11 Number of HSP's that attempted gapping in prelim test: 213 Number of HSP's gapped (non-prelim): 17 length of query: 91 length of database: 10,336,785 effective HSP length: 60 effective length of query: 31 effective length of database: 8,138,325 effective search space: 252288075 effective search space used: 252288075 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 52 (24.7 bits)