BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9C0B1 (505 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4IE5-A nrPDB 996 0.0 3BOR-A nrPDB 33 0.21 1QHL-A nrPDB 31 1.2 3EIQ-A nrPDB 30 2.1 5ZBZ-A nrPDB 30 2.5 3PJ9-A nrPDB 28 6.5 >4IE5-A nrPDB Length = 495 Score = 996 bits (2574), Expect = 0.0, Method: Compositional matrix adjust. Identities = 474/476 (99%), Positives = 476/476 (100%) Query: 30 YLTPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKD 89 ++TPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKD Sbjct: 20 HMTPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKD 79 Query: 90 LLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSNIKHTEAEIAAACETFLKLNDYLQIE 149 LLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSNIKHTEAEIAAACETFLKLNDYLQIE Sbjct: 80 LLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSNIKHTEAEIAAACETFLKLNDYLQIE 139 Query: 150 TIQALEELAAKEKANEDAVPLCMSADFPRVGMGSSYNGQDEVDIKSRAAYNVTLLNFMDP 209 TIQALEELAAKEKANEDAVPLCMSADFPRVGMGSSYNGQDEVDIKSRAAYNVTLLNFMDP Sbjct: 140 TIQALEELAAKEKANEDAVPLCMSADFPRVGMGSSYNGQDEVDIKSRAAYNVTLLNFMDP 199 Query: 210 QKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGPEEESEDDSHLEGRDPDI 269 QKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGPEEESEDDSHLEGRDPDI Sbjct: 200 QKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGPEEESEDDSHLEGRDPDI 259 Query: 270 WHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGSQPRFSSTHRVAECSTG 329 WHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGSQPRFSSTHRVAECSTG Sbjct: 260 WHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGSQPRFSSTHRVAECSTG 319 Query: 330 TLDYILQRCQLALQNVCDDVDNDDVSLKSFEPAVLKQGEEIHNEVEFEWLRQFWFQGNRY 389 TLDYILQRCQLALQNVCDDVDNDDVSLKSFEPAVLKQGEEIHNEVEFEWLRQFWFQGNRY Sbjct: 320 TLDYILQRCQLALQNVCDDVDNDDVSLKSFEPAVLKQGEEIHNEVEFEWLRQFWFQGNRY 379 Query: 390 RKCTDWWCQPMAQLEALWKKMEGVTNAVLHEVKREGLPVEQRNEILTAILASLTARQNLR 449 RKCTDWWCQPMAQLEALWKKMEGVTNAVLHEVKREGLPVEQRNEILTAILASLTARQNLR Sbjct: 380 RKCTDWWCQPMAQLEALWKKMEGVTNAVLHEVKREGLPVEQRNEILTAILASLTARQNLR 439 Query: 450 REWHARCQSRIARTLPADQKPECRPYWEKDDASMPLPFDLTDIVSELRGQLLEAKP 505 REWHARCQSRIARTLPADQKPECRPYWEKDDASMPLPFDLTDIVSELRGQLLEAKP Sbjct: 440 REWHARCQSRIARTLPADQKPECRPYWEKDDASMPLPFDLTDIVSELRGQLLEAKP 495 >3BOR-A nrPDB Length = 237 Score = 33.5 bits (75), Expect = 0.21, Method: Compositional matrix adjust. Identities = 26/107 (24%), Positives = 46/107 (42%), Gaps = 12/107 (11%) Query: 58 SEELHKEVQEAFLTLHK------HGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNT 111 + EL +++Q+ L L H C+ VR + + L I++G PG + LN Sbjct: 107 TRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNR 166 Query: 112 RLFTVPWPVKGSNIKHTEAEIAAAC-----ETFLKLNDYLQIETIQA 153 R + W +K + + ++ E F KLN +Q+ + A Sbjct: 167 RYLSPKW-IKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSA 212 >1QHL-A nrPDB Length = 227 Score = 31.2 bits (69), Expect = 1.2, Method: Compositional matrix adjust. Identities = 13/28 (46%), Positives = 16/28 (57%) Query: 284 GLAIPLHQGDCYFMLDDLNATHQHCVLA 311 GL L G CY MLD +N+ HQ V+ Sbjct: 76 GLHGKLKAGVCYSMLDTINSRHQRVVVG 103 >3EIQ-A nrPDB Length = 414 Score = 30.4 bits (67), Expect = 2.1, Method: Compositional matrix adjust. Identities = 24/107 (22%), Positives = 45/107 (42%), Gaps = 12/107 (11%) Query: 58 SEELHKEVQEAFLTLHK------HGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNT 111 + EL +++Q+ + L H C+ VR + + L I++G PG + LN Sbjct: 117 TRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNR 176 Query: 112 RLFTVPWPVKGSNIKHTEAEIAAAC-----ETFLKLNDYLQIETIQA 153 R + P +K + + ++ + F KLN Q+ + A Sbjct: 177 RYLS-PKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSA 222 >5ZBZ-A nrPDB Length = 220 Score = 30.0 bits (66), Expect = 2.5, Method: Compositional matrix adjust. Identities = 16/64 (25%), Positives = 29/64 (45%), Gaps = 6/64 (9%) Query: 58 SEELHKEVQEAFLTLHK------HGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNT 111 + EL +++Q+ + L H C+ VR + + L I++G PG + LN Sbjct: 91 TRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNR 150 Query: 112 RLFT 115 R + Sbjct: 151 RYLS 154 >3PJ9-A nrPDB Length = 140 Score = 28.5 bits (62), Expect = 6.5, Method: Composition-based stats. Identities = 24/81 (29%), Positives = 33/81 (40%), Gaps = 3/81 (3%) Query: 51 LREASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLN 110 LR A+ +L KE E F +HK F+DLV + PV ++ G K + Sbjct: 33 LRIAAMKKVQLSKEQAENFYAVHKERPFFKDLVEFM---ISGPVVVSILEGEGAVLKNRD 89 Query: 111 TRLFTVPWPVKGSNIKHTEAE 131 T P K I+ AE Sbjct: 90 LMGATNPKEAKAGTIRADFAE 110 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4IE5-A nrPDB 1013 0.0 Sequences not found previously or not previously below threshold: 3BOR-A nrPDB 34 0.12 5ZBZ-A nrPDB 30 2.9 2WYM-A nrPDB 30 3.2 3EIQ-A nrPDB 30 3.4 1QHL-A nrPDB 29 4.1 3PJ9-A nrPDB 29 5.2 2VNU-D nrPDB 28 7.1 CONVERGED! >4IE5-A nrPDB Length = 495 Score = 1013 bits (2619), Expect = 0.0, Method: Composition-based stats. Identities = 474/476 (99%), Positives = 476/476 (100%) Query: 30 YLTPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKD 89 ++TPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKD Sbjct: 20 HMTPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKD 79 Query: 90 LLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSNIKHTEAEIAAACETFLKLNDYLQIE 149 LLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSNIKHTEAEIAAACETFLKLNDYLQIE Sbjct: 80 LLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSNIKHTEAEIAAACETFLKLNDYLQIE 139 Query: 150 TIQALEELAAKEKANEDAVPLCMSADFPRVGMGSSYNGQDEVDIKSRAAYNVTLLNFMDP 209 TIQALEELAAKEKANEDAVPLCMSADFPRVGMGSSYNGQDEVDIKSRAAYNVTLLNFMDP Sbjct: 140 TIQALEELAAKEKANEDAVPLCMSADFPRVGMGSSYNGQDEVDIKSRAAYNVTLLNFMDP 199 Query: 210 QKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGPEEESEDDSHLEGRDPDI 269 QKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGPEEESEDDSHLEGRDPDI Sbjct: 200 QKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGPEEESEDDSHLEGRDPDI 259 Query: 270 WHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGSQPRFSSTHRVAECSTG 329 WHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGSQPRFSSTHRVAECSTG Sbjct: 260 WHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGSQPRFSSTHRVAECSTG 319 Query: 330 TLDYILQRCQLALQNVCDDVDNDDVSLKSFEPAVLKQGEEIHNEVEFEWLRQFWFQGNRY 389 TLDYILQRCQLALQNVCDDVDNDDVSLKSFEPAVLKQGEEIHNEVEFEWLRQFWFQGNRY Sbjct: 320 TLDYILQRCQLALQNVCDDVDNDDVSLKSFEPAVLKQGEEIHNEVEFEWLRQFWFQGNRY 379 Query: 390 RKCTDWWCQPMAQLEALWKKMEGVTNAVLHEVKREGLPVEQRNEILTAILASLTARQNLR 449 RKCTDWWCQPMAQLEALWKKMEGVTNAVLHEVKREGLPVEQRNEILTAILASLTARQNLR Sbjct: 380 RKCTDWWCQPMAQLEALWKKMEGVTNAVLHEVKREGLPVEQRNEILTAILASLTARQNLR 439 Query: 450 REWHARCQSRIARTLPADQKPECRPYWEKDDASMPLPFDLTDIVSELRGQLLEAKP 505 REWHARCQSRIARTLPADQKPECRPYWEKDDASMPLPFDLTDIVSELRGQLLEAKP Sbjct: 440 REWHARCQSRIARTLPADQKPECRPYWEKDDASMPLPFDLTDIVSELRGQLLEAKP 495 >3BOR-A nrPDB Length = 237 Score = 34.2 bits (77), Expect = 0.12, Method: Composition-based stats. Identities = 26/107 (24%), Positives = 46/107 (42%), Gaps = 12/107 (11%) Query: 58 SEELHKEVQEAFLTLHK------HGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNT 111 + EL +++Q+ L L H C+ VR + + L I++G PG + LN Sbjct: 107 TRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNR 166 Query: 112 RLFTVPWPVKGSNIKHTEAEIAAAC-----ETFLKLNDYLQIETIQA 153 R + W +K + + ++ E F KLN +Q+ + A Sbjct: 167 RYLSPKW-IKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSA 212 >5ZBZ-A nrPDB Length = 220 Score = 30.0 bits (66), Expect = 2.9, Method: Composition-based stats. Identities = 16/64 (25%), Positives = 29/64 (45%), Gaps = 6/64 (9%) Query: 58 SEELHKEVQEAFLTLHK------HGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNT 111 + EL +++Q+ + L H C+ VR + + L I++G PG + LN Sbjct: 91 TRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNR 150 Query: 112 RLFT 115 R + Sbjct: 151 RYLS 154 >2WYM-A nrPDB Length = 360 Score = 29.6 bits (65), Expect = 3.2, Method: Composition-based stats. Identities = 22/85 (25%), Positives = 35/85 (41%), Gaps = 17/85 (20%) Query: 224 GKMAVSWHHDENLVDRSAVAVYSYSCEGPEE-----ESEDDSHLEGRDPDIWHVGFKISW 278 K+ + +HHD ++S P+E E + D G P IW VG K +W Sbjct: 274 AKVVIPFHHD----------IWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTW 323 Query: 279 DIETPGLAIPLHQG--DCYFMLDDL 301 ++ +G DC+ + DL Sbjct: 324 PLDKDNFEYHYPRGFDDCFTIEPDL 348 >3EIQ-A nrPDB Length = 414 Score = 29.6 bits (65), Expect = 3.4, Method: Composition-based stats. Identities = 16/64 (25%), Positives = 29/64 (45%), Gaps = 6/64 (9%) Query: 58 SEELHKEVQEAFLTLHK------HGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNT 111 + EL +++Q+ + L H C+ VR + + L I++G PG + LN Sbjct: 117 TRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNR 176 Query: 112 RLFT 115 R + Sbjct: 177 RYLS 180 >1QHL-A nrPDB Length = 227 Score = 29.2 bits (64), Expect = 4.1, Method: Composition-based stats. Identities = 13/28 (46%), Positives = 16/28 (57%) Query: 284 GLAIPLHQGDCYFMLDDLNATHQHCVLA 311 GL L G CY MLD +N+ HQ V+ Sbjct: 76 GLHGKLKAGVCYSMLDTINSRHQRVVVG 103 >3PJ9-A nrPDB Length = 140 Score = 28.8 bits (63), Expect = 5.2, Method: Composition-based stats. Identities = 24/81 (29%), Positives = 33/81 (40%), Gaps = 3/81 (3%) Query: 51 LREASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLN 110 LR A+ +L KE E F +HK F+DLV + PV ++ G K + Sbjct: 33 LRIAAMKKVQLSKEQAENFYAVHKERPFFKDLVEFM---ISGPVVVSILEGEGAVLKNRD 89 Query: 111 TRLFTVPWPVKGSNIKHTEAE 131 T P K I+ AE Sbjct: 90 LMGATNPKEAKAGTIRADFAE 110 >2VNU-D nrPDB Length = 760 Score = 28.4 bits (62), Expect = 7.1, Method: Composition-based stats. Identities = 34/127 (26%), Positives = 62/127 (48%), Gaps = 17/127 (13%) Query: 97 ILIGNPGCTYK-YLNTRLFTVPWPVKGS----NIKHTEAEIAAACETF------LKLNDY 145 +L+G C+ K Y++ F+V W + S N+ ++ I + E F L+++D Sbjct: 365 MLLGTDLCSLKPYVDRFAFSVIWELDDSANIVNVNFMKSVIRSR-EAFSYEQAQLRIDDK 423 Query: 146 LQIETI----QALEELAAKEKANE-DAVPLCMSADFPRVGMGSSYNGQDEVDIKSRAAYN 200 Q + + +AL +L+ K K +A L +++ +V M S + +EV+IK A N Sbjct: 424 TQNDELTMGMRALLKLSVKLKQKRLEAGALNLASPEVKVHMDSETSDPNEVEIKKLLATN 483 Query: 201 VTLLNFM 207 + FM Sbjct: 484 SLVEEFM 490 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.314 0.141 0.459 Lambda K H 0.267 0.0411 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 21,967,609 Number of Sequences: 36641 Number of extensions: 961221 Number of successful extensions: 2170 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 16 Number of HSP's that attempted gapping in prelim test: 2164 Number of HSP's gapped (non-prelim): 22 length of query: 505 length of database: 10,336,785 effective HSP length: 101 effective length of query: 404 effective length of database: 6,636,044 effective search space: 2680961776 effective search space used: 2680961776 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.4 bits) S2: 61 (28.1 bits)