BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9C005 (99 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6E2H-E nrPDB 201 7e-53 4RT4-A nrPDB 120 2e-28 6CHG-E nrPDB 37 0.002 3D79-A nrPDB 27 1.9 3CSQ-A nrPDB 26 4.2 >6E2H-E nrPDB Length = 100 Score = 201 bits (510), Expect = 7e-53, Method: Compositional matrix adjust. Identities = 99/99 (100%), Positives = 99/99 (100%) Query: 1 MEPEQMLEGQTQVAENPHSEYGLTDNVERIVENEKINAEKSSKQKVDLQSLPTRAYLDQT 60 MEPEQMLEGQTQVAENPHSEYGLTDNVERIVENEKINAEKSSKQKVDLQSLPTRAYLDQT Sbjct: 2 MEPEQMLEGQTQVAENPHSEYGLTDNVERIVENEKINAEKSSKQKVDLQSLPTRAYLDQT 61 Query: 61 VVPILLQGLAVLAKERPPNPIEFLASYLLKNKAQFEDRN 99 VVPILLQGLAVLAKERPPNPIEFLASYLLKNKAQFEDRN Sbjct: 62 VVPILLQGLAVLAKERPPNPIEFLASYLLKNKAQFEDRN 100 >4RT4-A nrPDB Length = 66 Score = 120 bits (300), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 59/59 (100%), Positives = 59/59 (100%) Query: 41 SSKQKVDLQSLPTRAYLDQTVVPILLQGLAVLAKERPPNPIEFLASYLLKNKAQFEDRN 99 SSKQKVDLQSLPTRAYLDQTVVPILLQGLAVLAKERPPNPIEFLASYLLKNKAQFEDRN Sbjct: 1 SSKQKVDLQSLPTRAYLDQTVVPILLQGLAVLAKERPPNPIEFLASYLLKNKAQFEDRN 59 >6CHG-E nrPDB Length = 61 Score = 37.0 bits (84), Expect = 0.002, Method: Compositional matrix adjust. Identities = 14/37 (37%), Positives = 26/37 (70%) Query: 53 TRAYLDQTVVPILLQGLAVLAKERPPNPIEFLASYLL 89 TR YL++ V LL+G+ ++A+E+P +P+ L +L+ Sbjct: 14 TRRYLNEHVTKHLLEGMKLIAREKPEDPLRVLGQFLI 50 >3D79-A nrPDB Length = 179 Score = 26.9 bits (58), Expect = 1.9, Method: Compositional matrix adjust. Identities = 11/27 (40%), Positives = 16/27 (59%) Query: 47 DLQSLPTRAYLDQTVVPILLQGLAVLA 73 DL+ P R +D+ VP +L G V+A Sbjct: 89 DLRKWPRRVVVDEGAVPHILNGADVMA 115 >3CSQ-A nrPDB Length = 334 Score = 25.8 bits (55), Expect = 4.2, Method: Composition-based stats. Identities = 11/40 (27%), Positives = 22/40 (55%) Query: 41 SSKQKVDLQSLPTRAYLDQTVVPILLQGLAVLAKERPPNP 80 ++ Q ++L+ + + Y+ T P L + + + ERP NP Sbjct: 103 NNAQWINLRDMTFKEYIKSTKTPRELAMIFLASYERPANP 142 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6E2H-E nrPDB 189 2e-49 4RT4-A nrPDB 117 8e-28 Sequences not found previously or not previously below threshold: 6CHG-E nrPDB 39 7e-04 2EP5-A nrPDB 28 0.97 3NYS-A nrPDB 27 1.5 3D79-A nrPDB 27 2.3 3CSQ-A nrPDB 27 2.7 5OCR-A nrPDB 26 2.8 3NWN-A nrPDB 26 4.2 >6E2H-E nrPDB Length = 100 Score = 189 bits (481), Expect = 2e-49, Method: Composition-based stats. Identities = 99/99 (100%), Positives = 99/99 (100%) Query: 1 MEPEQMLEGQTQVAENPHSEYGLTDNVERIVENEKINAEKSSKQKVDLQSLPTRAYLDQT 60 MEPEQMLEGQTQVAENPHSEYGLTDNVERIVENEKINAEKSSKQKVDLQSLPTRAYLDQT Sbjct: 2 MEPEQMLEGQTQVAENPHSEYGLTDNVERIVENEKINAEKSSKQKVDLQSLPTRAYLDQT 61 Query: 61 VVPILLQGLAVLAKERPPNPIEFLASYLLKNKAQFEDRN 99 VVPILLQGLAVLAKERPPNPIEFLASYLLKNKAQFEDRN Sbjct: 62 VVPILLQGLAVLAKERPPNPIEFLASYLLKNKAQFEDRN 100 >4RT4-A nrPDB Length = 66 Score = 117 bits (294), Expect = 8e-28, Method: Composition-based stats. Identities = 59/59 (100%), Positives = 59/59 (100%) Query: 41 SSKQKVDLQSLPTRAYLDQTVVPILLQGLAVLAKERPPNPIEFLASYLLKNKAQFEDRN 99 SSKQKVDLQSLPTRAYLDQTVVPILLQGLAVLAKERPPNPIEFLASYLLKNKAQFEDRN Sbjct: 1 SSKQKVDLQSLPTRAYLDQTVVPILLQGLAVLAKERPPNPIEFLASYLLKNKAQFEDRN 59 >6CHG-E nrPDB Length = 61 Score = 38.6 bits (88), Expect = 7e-04, Method: Composition-based stats. Identities = 14/37 (37%), Positives = 26/37 (70%) Query: 53 TRAYLDQTVVPILLQGLAVLAKERPPNPIEFLASYLL 89 TR YL++ V LL+G+ ++A+E+P +P+ L +L+ Sbjct: 14 TRRYLNEHVTKHLLEGMKLIAREKPEDPLRVLGQFLI 50 >2EP5-A nrPDB Length = 350 Score = 27.8 bits (60), Expect = 0.97, Method: Composition-based stats. Identities = 20/55 (36%), Positives = 27/55 (49%), Gaps = 4/55 (7%) Query: 27 VERIVENEKINAEKSSKQKVDLQSLPTRAYLDQTVVPILLQGLAVLAKERPPNPI 81 V IV NE+IN E+ K + +SLP + L P Q + V +E P PI Sbjct: 247 VINIVTNERINIEEIKKTLKNFKSLPQQKNL--PTAP--KQPIIVRDEEDRPQPI 297 >3NYS-A nrPDB Length = 367 Score = 27.4 bits (59), Expect = 1.5, Method: Composition-based stats. Identities = 18/73 (24%), Positives = 30/73 (41%), Gaps = 1/73 (1%) Query: 1 MEPEQMLEGQTQVAENPHS-EYGLTDNVERIVENEKINAEKSSKQKVDLQSLPTRAYLDQ 59 M+ + ++ + A P + Y + N + V +EK K + SLP YLD Sbjct: 288 MDNRESVQASLKAAGVPTAVHYPIPLNKQPAVADEKAKLPVGDKAATQVMSLPMHPYLDT 347 Query: 60 TVVPILLQGLAVL 72 + I+ L L Sbjct: 348 ASIKIICAALTNL 360 >3D79-A nrPDB Length = 179 Score = 26.6 bits (57), Expect = 2.3, Method: Composition-based stats. Identities = 11/27 (40%), Positives = 16/27 (59%) Query: 47 DLQSLPTRAYLDQTVVPILLQGLAVLA 73 DL+ P R +D+ VP +L G V+A Sbjct: 89 DLRKWPRRVVVDEGAVPHILNGADVMA 115 >3CSQ-A nrPDB Length = 334 Score = 26.6 bits (57), Expect = 2.7, Method: Composition-based stats. Identities = 11/40 (27%), Positives = 22/40 (55%) Query: 41 SSKQKVDLQSLPTRAYLDQTVVPILLQGLAVLAKERPPNP 80 ++ Q ++L+ + + Y+ T P L + + + ERP NP Sbjct: 103 NNAQWINLRDMTFKEYIKSTKTPRELAMIFLASYERPANP 142 >5OCR-A nrPDB Length = 286 Score = 26.2 bits (56), Expect = 2.8, Method: Composition-based stats. Identities = 10/27 (37%), Positives = 15/27 (55%) Query: 32 ENEKINAEKSSKQKVDLQSLPTRAYLD 58 +N IN E +K + L +PT Y+D Sbjct: 252 KNNAINPETDAKAREKLSDIPTSMYVD 278 >3NWN-A nrPDB Length = 359 Score = 25.9 bits (55), Expect = 4.2, Method: Composition-based stats. Identities = 14/45 (31%), Positives = 25/45 (55%), Gaps = 1/45 (2%) Query: 53 TRAYLDQTVVPILLQGLAVLAKERPPNPIEFLASYL-LKNKAQFE 96 T Y + ++P LQ + + +ERP + I SYL + N++ F+ Sbjct: 126 TENYKHRGILPRALQQVFRMIEERPTHAITVRVSYLEIYNESLFD 170 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.309 0.129 0.345 Lambda K H 0.267 0.0397 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,652,400 Number of Sequences: 36641 Number of extensions: 116752 Number of successful extensions: 234 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 17 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 217 Number of HSP's gapped (non-prelim): 21 length of query: 99 length of database: 10,336,785 effective HSP length: 67 effective length of query: 32 effective length of database: 7,881,838 effective search space: 252218816 effective search space used: 252218816 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 52 (24.7 bits)