BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9BUL9 (199 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6AHR-F nrPDB 398 e-112 5IA9-A nrPDB 27 8.5 >6AHR-F nrPDB Length = 199 Score = 398 bits (1023), Expect = e-112, Method: Compositional matrix adjust. Identities = 199/199 (100%), Positives = 199/199 (100%) Query: 1 MENFRKVRSEEAPAGCGAEGGGPGSGPFADLAPGAVHMRVKEGSKIRNLMAFATASMAQP 60 MENFRKVRSEEAPAGCGAEGGGPGSGPFADLAPGAVHMRVKEGSKIRNLMAFATASMAQP Sbjct: 1 MENFRKVRSEEAPAGCGAEGGGPGSGPFADLAPGAVHMRVKEGSKIRNLMAFATASMAQP 60 Query: 61 ATRAIVFSGCGRATTKTVTCAEILKRRLAGLHQVTRLRYRSVREVWQSLPPGPTQGQTPG 120 ATRAIVFSGCGRATTKTVTCAEILKRRLAGLHQVTRLRYRSVREVWQSLPPGPTQGQTPG Sbjct: 61 ATRAIVFSGCGRATTKTVTCAEILKRRLAGLHQVTRLRYRSVREVWQSLPPGPTQGQTPG 120 Query: 121 EPAASLSVLKNVPGLAILLSKDALDPRQPGYQPPNPHPGPSSPPAAPASKRSLGEPAAGE 180 EPAASLSVLKNVPGLAILLSKDALDPRQPGYQPPNPHPGPSSPPAAPASKRSLGEPAAGE Sbjct: 121 EPAASLSVLKNVPGLAILLSKDALDPRQPGYQPPNPHPGPSSPPAAPASKRSLGEPAAGE 180 Query: 181 GSAKRSQPEPGVADEDQTA 199 GSAKRSQPEPGVADEDQTA Sbjct: 181 GSAKRSQPEPGVADEDQTA 199 >5IA9-A nrPDB Length = 155 Score = 26.6 bits (57), Expect = 8.5, Method: Compositional matrix adjust. Identities = 11/30 (36%), Positives = 19/30 (63%), Gaps = 2/30 (6%) Query: 25 SGPFADLAPGAVHMRVKEGSKIRNLMAFAT 54 SGP DL+ +H R+ G++I + +A+ T Sbjct: 96 SGP--DLSTALIHFRIFVGARIYHTIAYLT 123 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6AHR-F nrPDB 349 6e-97 Sequences not found previously or not previously below threshold: 4U5I-A nrPDB 30 0.69 6BSD-A nrPDB 30 0.94 2X26-A nrPDB 29 2.2 6O9M-2 nrPDB 29 2.2 CONVERGED! >6AHR-F nrPDB Length = 199 Score = 349 bits (894), Expect = 6e-97, Method: Composition-based stats. Identities = 199/199 (100%), Positives = 199/199 (100%) Query: 1 MENFRKVRSEEAPAGCGAEGGGPGSGPFADLAPGAVHMRVKEGSKIRNLMAFATASMAQP 60 MENFRKVRSEEAPAGCGAEGGGPGSGPFADLAPGAVHMRVKEGSKIRNLMAFATASMAQP Sbjct: 1 MENFRKVRSEEAPAGCGAEGGGPGSGPFADLAPGAVHMRVKEGSKIRNLMAFATASMAQP 60 Query: 61 ATRAIVFSGCGRATTKTVTCAEILKRRLAGLHQVTRLRYRSVREVWQSLPPGPTQGQTPG 120 ATRAIVFSGCGRATTKTVTCAEILKRRLAGLHQVTRLRYRSVREVWQSLPPGPTQGQTPG Sbjct: 61 ATRAIVFSGCGRATTKTVTCAEILKRRLAGLHQVTRLRYRSVREVWQSLPPGPTQGQTPG 120 Query: 121 EPAASLSVLKNVPGLAILLSKDALDPRQPGYQPPNPHPGPSSPPAAPASKRSLGEPAAGE 180 EPAASLSVLKNVPGLAILLSKDALDPRQPGYQPPNPHPGPSSPPAAPASKRSLGEPAAGE Sbjct: 121 EPAASLSVLKNVPGLAILLSKDALDPRQPGYQPPNPHPGPSSPPAAPASKRSLGEPAAGE 180 Query: 181 GSAKRSQPEPGVADEDQTA 199 GSAKRSQPEPGVADEDQTA Sbjct: 181 GSAKRSQPEPGVADEDQTA 199 >4U5I-A nrPDB Length = 403 Score = 30.1 bits (66), Expect = 0.69, Method: Composition-based stats. Identities = 10/20 (50%), Positives = 12/20 (60%), Gaps = 1/20 (5%) Query: 149 PGYQP-PNPHPGPSSPPAAP 167 P Y P P+P P P+ PP P Sbjct: 384 PSYSPKPSPTPRPTKPPVTP 403 >6BSD-A nrPDB Length = 876 Score = 29.7 bits (65), Expect = 0.94, Method: Composition-based stats. Identities = 24/68 (35%), Positives = 31/68 (45%), Gaps = 11/68 (16%) Query: 126 LSVLKNVPGLAILLSKDALDPRQPG-YQPPNPHPGPSSPPAAPASKRSLGEPAAGEGSAK 184 L+V +VPG IL++ PR+P YQ P P P P +AP P S Sbjct: 459 LTVHLSVPGDTILINNRP-GPREPPPYQEPRPRGNP--PHSAPCV------PNGSAYSGD 509 Query: 185 RSQPE-PG 191 +PE PG Sbjct: 510 YMEPEKPG 517 >2X26-A nrPDB Length = 308 Score = 28.5 bits (62), Expect = 2.2, Method: Composition-based stats. Identities = 18/69 (26%), Positives = 25/69 (36%), Gaps = 2/69 (2%) Query: 88 LAGLHQVTRLRYRSVREVWQSLPPGPTQGQTPGEPAASLSVLKNVPGLAILLSKDALDPR 147 LA HQ+ RY + W P GP + + L ++P I D Sbjct: 18 LAKSHQLLEKRYPESKISWVEFPAGPQMLEALNVGSIDLGSTGDIP--PIFAQAAGADLV 75 Query: 148 QPGYQPPNP 156 G +PP P Sbjct: 76 YVGVEPPKP 84 >6O9M-2 nrPDB Length = 462 Score = 28.5 bits (62), Expect = 2.2, Method: Composition-based stats. Identities = 17/47 (36%), Positives = 27/47 (57%), Gaps = 2/47 (4%) Query: 90 GLHQVTRLRYRSVREVWQSLPPGPTQGQTPGEPAASLSVLKNVPGLA 136 GL++V L+ R+++E L PG + G PA L+V + +P LA Sbjct: 8 GLNRV-HLQCRNLQEFLGGLSPG-VLDRLYGHPATCLAVFRELPSLA 52 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.312 0.130 0.387 Lambda K H 0.267 0.0404 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,819,891 Number of Sequences: 36641 Number of extensions: 359155 Number of successful extensions: 1028 Number of sequences better than 10.0: 33 Number of HSP's better than 10.0 without gapping: 34 Number of HSP's successfully gapped in prelim test: 34 Number of HSP's that attempted gapping in prelim test: 879 Number of HSP's gapped (non-prelim): 158 length of query: 199 length of database: 10,336,785 effective HSP length: 92 effective length of query: 107 effective length of database: 6,965,813 effective search space: 745341991 effective search space used: 745341991 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 56 (26.2 bits)