BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9BUH6 (204 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3WTF-A nrPDB 401 e-112 3WTD-A nrPDB 325 9e-90 4WJA-A nrPDB 288 9e-79 4A7Z-A nrPDB 30 0.57 3S81-A nrPDB 27 5.8 3RSY-A nrPDB 27 6.5 2CQS-A nrPDB 27 6.7 6IAA-A nrPDB 27 7.9 >3WTF-A nrPDB Length = 206 Score = 401 bits (1030), Expect = e-112, Method: Compositional matrix adjust. Identities = 204/204 (100%), Positives = 204/204 (100%) Query: 1 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL 60 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL Sbjct: 3 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL 62 Query: 61 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR 120 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR Sbjct: 63 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR 122 Query: 121 LRALTLGLAKRVWSLERRLAAAEETAVSPRKSPRPAGPQLFLPDPDPQRGGPGPGVRRRC 180 LRALTLGLAKRVWSLERRLAAAEETAVSPRKSPRPAGPQLFLPDPDPQRGGPGPGVRRRC Sbjct: 123 LRALTLGLAKRVWSLERRLAAAEETAVSPRKSPRPAGPQLFLPDPDPQRGGPGPGVRRRC 182 Query: 181 PGESLINPGFKSKKPAGGVDFDET 204 PGESLINPGFKSKKPAGGVDFDET Sbjct: 183 PGESLINPGFKSKKPAGGVDFDET 206 >3WTD-A nrPDB Length = 168 Score = 325 bits (833), Expect = 9e-90, Method: Compositional matrix adjust. Identities = 166/166 (100%), Positives = 166/166 (100%) Query: 1 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL 60 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL Sbjct: 3 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL 62 Query: 61 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR 120 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR Sbjct: 63 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR 122 Query: 121 LRALTLGLAKRVWSLERRLAAAEETAVSPRKSPRPAGPQLFLPDPD 166 LRALTLGLAKRVWSLERRLAAAEETAVSPRKSPRPAGPQLFLPDPD Sbjct: 123 LRALTLGLAKRVWSLERRLAAAEETAVSPRKSPRPAGPQLFLPDPD 168 >4WJA-A nrPDB Length = 161 Score = 288 bits (738), Expect = 9e-79, Method: Compositional matrix adjust. Identities = 145/145 (100%), Positives = 145/145 (100%) Query: 1 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL 60 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL Sbjct: 17 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL 76 Query: 61 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR 120 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR Sbjct: 77 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR 136 Query: 121 LRALTLGLAKRVWSLERRLAAAEET 145 LRALTLGLAKRVWSLERRLAAAEET Sbjct: 137 LRALTLGLAKRVWSLERRLAAAEET 161 >4A7Z-A nrPDB Length = 900 Score = 30.4 bits (67), Expect = 0.57, Method: Compositional matrix adjust. Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 8/60 (13%) Query: 82 QQAVALTLQEDRAS--------LTLSGGPSALAFDLSKVPGPEAAPRLRALTLGLAKRVW 133 Q +L LQ++ A+ L L GGPS A+++S VPG + A T L W Sbjct: 759 HQTASLALQKNDATDGTPVLALLDLDGGPSPQAWNISHVPGTDMYEIAHAKTGSLVCARW 818 >3S81-A nrPDB Length = 268 Score = 26.9 bits (58), Expect = 5.8, Method: Compositional matrix adjust. Identities = 19/60 (31%), Positives = 29/60 (48%), Gaps = 15/60 (25%) Query: 59 ALKARFGL------SAAEDITPRF----RAACEQQAVALTLQE-----DRASLTLSGGPS 103 A+K G+ +A D+ +F A+C+QQ + L + DR + LSGGPS Sbjct: 24 AMKHTIGILGGMGPAATADMLEKFVELRHASCDQQHIPLIVSSIPDIPDRTACLLSGGPS 83 >3RSY-A nrPDB Length = 822 Score = 26.9 bits (58), Expect = 6.5, Method: Composition-based stats. Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 2/37 (5%) Query: 32 GDRGGFNLYVTDAAELWSTCFTP--DSLAALKARFGL 66 D GG LYV D ++W+ + P L +AR GL Sbjct: 65 ADAGGRYLYVNDGGDVWTPSWLPVKADLDHFEARHGL 101 >2CQS-A nrPDB Length = 842 Score = 26.9 bits (58), Expect = 6.7, Method: Compositional matrix adjust. Identities = 14/36 (38%), Positives = 19/36 (52%), Gaps = 2/36 (5%) Query: 33 DRGGFNLYVTDAAELWSTCFTP--DSLAALKARFGL 66 D GG LYV D ++W+ + P L +AR GL Sbjct: 86 DAGGRYLYVNDGGDVWTPSWLPVKADLDHFEARHGL 121 >6IAA-A nrPDB Length = 859 Score = 26.6 bits (57), Expect = 7.9, Method: Compositional matrix adjust. Identities = 12/38 (31%), Positives = 20/38 (52%) Query: 96 LTLSGGPSALAFDLSKVPGPEAAPRLRALTLGLAKRVW 133 +T SG + +DLS +PGP + P++ +T W Sbjct: 495 VTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSW 532 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3WTF-A nrPDB 374 e-104 3WTD-A nrPDB 305 1e-83 4WJA-A nrPDB 286 6e-78 Sequences not found previously or not previously below threshold: 4A7Z-A nrPDB 31 0.43 3S81-A nrPDB 30 0.68 4FW9-A nrPDB 30 0.86 1N9W-A nrPDB 30 0.89 4LLS-A nrPDB 29 1.4 5BSZ-A nrPDB 28 2.8 4FWV-A nrPDB 27 4.5 2FZV-A nrPDB 27 4.9 CONVERGED! >3WTF-A nrPDB Length = 206 Score = 374 bits (961), Expect = e-104, Method: Composition-based stats. Identities = 204/204 (100%), Positives = 204/204 (100%) Query: 1 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL 60 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL Sbjct: 3 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL 62 Query: 61 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR 120 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR Sbjct: 63 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR 122 Query: 121 LRALTLGLAKRVWSLERRLAAAEETAVSPRKSPRPAGPQLFLPDPDPQRGGPGPGVRRRC 180 LRALTLGLAKRVWSLERRLAAAEETAVSPRKSPRPAGPQLFLPDPDPQRGGPGPGVRRRC Sbjct: 123 LRALTLGLAKRVWSLERRLAAAEETAVSPRKSPRPAGPQLFLPDPDPQRGGPGPGVRRRC 182 Query: 181 PGESLINPGFKSKKPAGGVDFDET 204 PGESLINPGFKSKKPAGGVDFDET Sbjct: 183 PGESLINPGFKSKKPAGGVDFDET 206 >3WTD-A nrPDB Length = 168 Score = 305 bits (780), Expect = 1e-83, Method: Composition-based stats. Identities = 166/166 (100%), Positives = 166/166 (100%) Query: 1 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL 60 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL Sbjct: 3 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL 62 Query: 61 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR 120 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR Sbjct: 63 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR 122 Query: 121 LRALTLGLAKRVWSLERRLAAAEETAVSPRKSPRPAGPQLFLPDPD 166 LRALTLGLAKRVWSLERRLAAAEETAVSPRKSPRPAGPQLFLPDPD Sbjct: 123 LRALTLGLAKRVWSLERRLAAAEETAVSPRKSPRPAGPQLFLPDPD 168 >4WJA-A nrPDB Length = 161 Score = 286 bits (731), Expect = 6e-78, Method: Composition-based stats. Identities = 145/145 (100%), Positives = 145/145 (100%) Query: 1 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL 60 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL Sbjct: 17 MDPLSPPLCTLPPGPEPPRFVCYCEGEESGEGDRGGFNLYVTDAAELWSTCFTPDSLAAL 76 Query: 61 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR 120 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR Sbjct: 77 KARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGPEAAPR 136 Query: 121 LRALTLGLAKRVWSLERRLAAAEET 145 LRALTLGLAKRVWSLERRLAAAEET Sbjct: 137 LRALTLGLAKRVWSLERRLAAAEET 161 >4A7Z-A nrPDB Length = 900 Score = 30.8 bits (68), Expect = 0.43, Method: Composition-based stats. Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 8/60 (13%) Query: 82 QQAVALTLQEDRAS--------LTLSGGPSALAFDLSKVPGPEAAPRLRALTLGLAKRVW 133 Q +L LQ++ A+ L L GGPS A+++S VPG + A T L W Sbjct: 759 HQTASLALQKNDATDGTPVLALLDLDGGPSPQAWNISHVPGTDMYEIAHAKTGSLVCARW 818 >3S81-A nrPDB Length = 268 Score = 30.0 bits (66), Expect = 0.68, Method: Composition-based stats. Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 9/48 (18%) Query: 65 GLSAAEDITPRF----RAACEQQAVALTLQE-----DRASLTLSGGPS 103 G +A D+ +F A+C+QQ + L + DR + LSGGPS Sbjct: 36 GPAATADMLEKFVELRHASCDQQHIPLIVSSIPDIPDRTACLLSGGPS 83 >4FW9-A nrPDB Length = 732 Score = 29.6 bits (65), Expect = 0.86, Method: Composition-based stats. Identities = 29/92 (31%), Positives = 37/92 (40%), Gaps = 16/92 (17%) Query: 66 LSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGP---EAAPRLR 122 L + RF+ A EQQ AL + A LS L PGP E + RL Sbjct: 125 LREKTQLEARFKEAREQQLEALRREAQEAGFALSTNGERLEL---TGPGPVPAELSARLE 181 Query: 123 ALTLGLAKRVWSLERRLAAAEETAVSPRKSPR 154 +TLG LAA+ E V+ R+ R Sbjct: 182 EVTLG----------SLAASAELEVALRRLRR 203 >1N9W-A nrPDB Length = 422 Score = 29.6 bits (65), Expect = 0.89, Method: Composition-based stats. Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 2/45 (4%) Query: 111 KVPGPEAAPRLRALTLGLAKRVWSLERRLAAAEETAVSPRKSPRP 155 K+P PE+A R+R L + AK LE + A E +SP P P Sbjct: 51 KLPLPESALRVRGLVVENAKAPGGLE--VQAKEVEVLSPALEPTP 93 >4LLS-A nrPDB Length = 311 Score = 28.8 bits (63), Expect = 1.4, Method: Composition-based stats. Identities = 24/64 (37%), Positives = 33/64 (51%), Gaps = 6/64 (9%) Query: 92 DRASLTLSGGP-SALAFDLSKVPGPEAAPRLRA-LTLGLAKRVWS----LERRLAAAEET 145 D A+ L+G LAF+L PG A+ +RA L L LA+ + L + L A ET Sbjct: 124 DDATAVLAGDALQTLAFELVTHPGASASAEVRADLALSLARASGAQGMVLGQALDIAAET 183 Query: 146 AVSP 149 A +P Sbjct: 184 ARAP 187 >5BSZ-A nrPDB Length = 250 Score = 28.1 bits (61), Expect = 2.8, Method: Composition-based stats. Identities = 27/107 (25%), Positives = 42/107 (39%), Gaps = 14/107 (13%) Query: 51 CFTPDSLAALKARFGLSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLS 110 C T + LA L F +A +++P RAA E++ A + + FDL Sbjct: 56 CGTGEHLAHLVEHFPDAAGVELSPAMRAAAERKLTATPVHQ----------ADMFDFDLG 105 Query: 111 KVPGP----EAAPRLRALTLGLAKRVWSLERRLAAAEETAVSPRKSP 153 +V + A T LA+ V ++ R L + P SP Sbjct: 106 RVFDAVCCLTGSIAYAADTAELARAVRAMARHLPVGGVLVIDPWWSP 152 >4FWV-A nrPDB Length = 355 Score = 27.3 bits (59), Expect = 4.5, Method: Composition-based stats. Identities = 28/92 (30%), Positives = 37/92 (40%), Gaps = 16/92 (17%) Query: 66 LSAAEDITPRFRAACEQQAVALTLQEDRASLTLSGGPSALAFDLSKVPGP---EAAPRLR 122 L + RF+ A EQQ AL + A LS L PGP E + RL Sbjct: 93 LREKTQLEARFKEAREQQLEALRREAQEAGFALSTNGERLEL---TGPGPVPAELSARLE 149 Query: 123 ALTLGLAKRVWSLERRLAAAEETAVSPRKSPR 154 +TLG +AA+ E V+ R+ R Sbjct: 150 EVTLG----------SMAASAELEVALRRLRR 171 >2FZV-A nrPDB Length = 279 Score = 27.3 bits (59), Expect = 4.9, Method: Composition-based stats. Identities = 34/123 (27%), Positives = 43/123 (34%), Gaps = 17/123 (13%) Query: 33 DRGGFNLYVTDAAELWSTCFTPDSLAALKARF-----GLSAAEDITP----RFRAACEQQ 83 D G NLY A PDSL AL F L A D P + + Sbjct: 12 DLGTENLYFQSNAMRLRHLSDPDSLPALDKSFAIERPALGLAPDAPPVRILLLYGSLRAR 71 Query: 84 AVALTLQEDRASLTLSGGPSALAFDLSKVPGPE--------AAPRLRALTLGLAKRVWSL 135 + + E+ A L G FD S +P P+ A LRAL+ +VW Sbjct: 72 SFSRLAVEEAARLLQFFGAETRIFDPSDLPLPDQVQSDDHPAVKELRALSEWSEGQVWCS 131 Query: 136 ERR 138 R Sbjct: 132 PER 134 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.137 0.425 Lambda K H 0.267 0.0421 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,659,015 Number of Sequences: 36641 Number of extensions: 410931 Number of successful extensions: 922 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 19 Number of HSP's that attempted gapping in prelim test: 904 Number of HSP's gapped (non-prelim): 37 length of query: 204 length of database: 10,336,785 effective HSP length: 92 effective length of query: 112 effective length of database: 6,965,813 effective search space: 780171056 effective search space used: 780171056 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 57 (26.5 bits)