BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9BU02 (230 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3TVL-A nrPDB 464 e-131 5A65-A nrPDB 320 3e-88 5A60-A nrPDB 37 0.007 1Z6A-A nrPDB 31 0.54 1TP7-A nrPDB 30 0.93 3IP1-A nrPDB 28 2.4 4K2H-A nrPDB 27 6.5 4ZW2-A nrPDB 27 6.7 5GJV-C nrPDB 27 8.3 4JDL-A nrPDB 27 9.8 >3TVL-A nrPDB Length = 230 Score = 464 bits (1195), Expect = e-131, Method: Compositional matrix adjust. Identities = 230/230 (100%), Positives = 230/230 (100%) Query: 1 MAQGLIEVERKFLPGPGTEERLQELGGTLEYRVTFRDTYYDTPELSLMQADHWLRRREDS 60 MAQGLIEVERKFLPGPGTEERLQELGGTLEYRVTFRDTYYDTPELSLMQADHWLRRREDS Sbjct: 1 MAQGLIEVERKFLPGPGTEERLQELGGTLEYRVTFRDTYYDTPELSLMQADHWLRRREDS 60 Query: 61 GWELKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPLGLQEVAS 120 GWELKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPLGLQEVAS Sbjct: 61 GWELKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPLGLQEVAS 120 Query: 121 FVTKRSAWKLVLLGADEEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSS 180 FVTKRSAWKLVLLGADEEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSS Sbjct: 121 FVTKRSAWKLVLLGADEEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSS 180 Query: 181 MLGVPAQETAPAKLIVYLQRFRPQDYQRLLEVNSSRERPQETEDPDHCLG 230 MLGVPAQETAPAKLIVYLQRFRPQDYQRLLEVNSSRERPQETEDPDHCLG Sbjct: 181 MLGVPAQETAPAKLIVYLQRFRPQDYQRLLEVNSSRERPQETEDPDHCLG 230 >5A65-A nrPDB Length = 217 Score = 320 bits (820), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 160/215 (74%), Positives = 177/215 (82%) Query: 1 MAQGLIEVERKFLPGPGTEERLQELGGTLEYRVTFRDTYYDTPELSLMQADHWLRRREDS 60 M QGLIEVERKF PGP TEERLQELG TLE+RVTFRDTYYDT ELSLM +DHWLR+RE S Sbjct: 3 MTQGLIEVERKFAPGPDTEERLQELGATLEHRVTFRDTYYDTSELSLMLSDHWLRQREGS 62 Query: 61 GWELKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPLGLQEVAS 120 GWELKCPG GV GPH EY E+T+E IVAQL ++L + VAAVLG L LQEVAS Sbjct: 63 GWELKCPGVTGVSGPHNEYVEVTSEAAIVAQLFELLGSGEQKPAGVAAVLGSLKLQEVAS 122 Query: 121 FVTKRSAWKLVLLGADEEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSS 180 F+T RS+WKL L GA +EPQL +DLD+ADFGYAVGEVEA+VHE+AEVP ALEKI +SS Sbjct: 123 FITTRSSWKLALSGAHGQEPQLTIDLDSADFGYAVGEVEAMVHEKAEVPAALEKIITVSS 182 Query: 181 MLGVPAQETAPAKLIVYLQRFRPQDYQRLLEVNSS 215 MLGVPAQE APAKL+VYLQRFRP DYQRLLE SS Sbjct: 183 MLGVPAQEEAPAKLMVYLQRFRPLDYQRLLEAASS 217 >5A60-A nrPDB Length = 435 Score = 37.0 bits (84), Expect = 0.007, Method: Compositional matrix adjust. Identities = 40/122 (32%), Positives = 58/122 (47%), Gaps = 12/122 (9%) Query: 1 MAQGLIEVERKFLPGPGTEERLQE----LGGTLEYRVTFRDTYYDTPELSLMQADHWLRR 56 MAQ E+E KF+ E L++ LGG V + YY+TP+ L D LR Sbjct: 3 MAQ---EIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETPDNWLRGHDMGLRI 59 Query: 57 REDSG---WELKCPG-AAGVLGPHTEYKELTAEPTI-VAQLCKVLRADGLGAGDVAAVLG 111 R ++G +K G G L EY +EPT+ +AQL + +G D+A+ + Sbjct: 60 RGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEVWPNGELPADLASRVQ 119 Query: 112 PL 113 PL Sbjct: 120 PL 121 >1Z6A-A nrPDB Length = 500 Score = 30.8 bits (68), Expect = 0.54, Method: Compositional matrix adjust. Identities = 29/143 (20%), Positives = 56/143 (39%), Gaps = 18/143 (12%) Query: 54 LRRREDSGWELKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPL 113 + + D W + G+LG ++E+K A P + + D + ++ A++ P Sbjct: 188 IENKVDDLWSIMTFLNPGLLGSYSEFKSKFATP--------IKKGDNMAKEELKAIISP- 238 Query: 114 GLQEVASFVTKRSAWKLVLLG--ADEEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTA 171 F+ +R+ + ++ D+ E + +L EVE L + V Sbjct: 239 -------FILRRTKYDKAIINDLPDKIETNVYCNLTPEQAAMYKAEVENLFNNIDSVTGI 291 Query: 172 LEKIHRLSSMLGVPAQETAPAKL 194 K LS++L + PA L Sbjct: 292 KRKGMILSTLLKLKQIVDHPALL 314 >1TP7-A nrPDB Length = 460 Score = 30.0 bits (66), Expect = 0.93, Method: Compositional matrix adjust. Identities = 13/35 (37%), Positives = 21/35 (60%) Query: 137 EEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTA 171 E++P+L+VD D+A F G E ++E +V A Sbjct: 45 EKDPRLKVDFDSALFSKYKGNTECSLNEHIQVAVA 79 >3IP1-A nrPDB Length = 404 Score = 28.5 bits (62), Expect = 2.4, Method: Compositional matrix adjust. Identities = 19/50 (38%), Positives = 27/50 (54%), Gaps = 3/50 (6%) Query: 85 EPTIVAQLCKVLRADGLGAGDVAAVL--GPLGLQEVASFVTKRSAWKLVL 132 EPT VA ++R G+ GD +L GP+GL VA + A K++L Sbjct: 195 EPTSVAYNAVIVRGGGIRPGDNVVILGGGPIGLAAVA-ILKHAGASKVIL 243 >4K2H-A nrPDB Length = 188 Score = 26.9 bits (58), Expect = 6.5, Method: Compositional matrix adjust. Identities = 33/110 (30%), Positives = 45/110 (40%), Gaps = 20/110 (18%) Query: 38 TYYDTPELSLMQADHWLRRREDSGWE-LKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVL 96 +Y+D P M AD L R+ + ++ + PG GP L A P ++A V Sbjct: 47 SYHDIP----MVADSTLSERQQALFDAVVLPG-----GPQGS-ANLAANPAVIA---FVA 93 Query: 97 RADGLG------AGDVAAVLGPLGLQEVASFVTKRSAWKLVLLGADEEEP 140 R D G A A VLG GL + +V WK V G + P Sbjct: 94 RHDAAGKLICPIASAAARVLGAHGLLKGRRYVCSGDLWKAVPEGVYVDAP 143 >4ZW2-A nrPDB Length = 333 Score = 26.9 bits (58), Expect = 6.7, Method: Compositional matrix adjust. Identities = 17/76 (22%), Positives = 40/76 (52%), Gaps = 2/76 (2%) Query: 138 EEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSSMLGVPAQ--ETAPAKLI 195 P + ++ ++ ++ EV++ + E+ L+ + + + PAQ +T+ A +I Sbjct: 198 NNPSKHIIIERSNTRSSLAEVQSEIERIFELARTLQLVALDADTINHPAQLSKTSLAPII 257 Query: 196 VYLQRFRPQDYQRLLE 211 VY++ P+ QRL++ Sbjct: 258 VYIKITSPKVLQRLIK 273 >5GJV-C nrPDB Length = 199 Score = 26.6 bits (57), Expect = 8.3, Method: Compositional matrix adjust. Identities = 17/76 (22%), Positives = 40/76 (52%), Gaps = 2/76 (2%) Query: 138 EEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSSMLGVPAQ--ETAPAKLI 195 P + ++ ++ ++ EV++ + E+ L+ + + + PAQ +T+ A +I Sbjct: 63 NNPSKHIIIERSNTRSSLAEVQSEIERIFELARTLQLVALDADTINHPAQLSKTSLAPII 122 Query: 196 VYLQRFRPQDYQRLLE 211 VY++ P+ QRL++ Sbjct: 123 VYIKITSPKVLQRLIK 138 >4JDL-A nrPDB Length = 223 Score = 26.6 bits (57), Expect = 9.8, Method: Compositional matrix adjust. Identities = 11/28 (39%), Positives = 18/28 (64%) Query: 93 CKVLRADGLGAGDVAAVLGPLGLQEVAS 120 C++++ DGL GD+ V+ GL V+S Sbjct: 113 CRIVQGDGLHVGDLREVMVVSGLPAVSS 140 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3TVL-A nrPDB 425 e-120 5A65-A nrPDB 387 e-108 Sequences not found previously or not previously below threshold: 5A60-A nrPDB 39 0.001 1TP7-A nrPDB 30 1.2 4K2H-A nrPDB 28 2.4 2RPI-A nrPDB 28 2.6 3IP1-A nrPDB 28 3.1 5VVI-A nrPDB 27 9.7 >3TVL-A nrPDB Length = 230 Score = 425 bits (1093), Expect = e-120, Method: Composition-based stats. Identities = 230/230 (100%), Positives = 230/230 (100%) Query: 1 MAQGLIEVERKFLPGPGTEERLQELGGTLEYRVTFRDTYYDTPELSLMQADHWLRRREDS 60 MAQGLIEVERKFLPGPGTEERLQELGGTLEYRVTFRDTYYDTPELSLMQADHWLRRREDS Sbjct: 1 MAQGLIEVERKFLPGPGTEERLQELGGTLEYRVTFRDTYYDTPELSLMQADHWLRRREDS 60 Query: 61 GWELKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPLGLQEVAS 120 GWELKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPLGLQEVAS Sbjct: 61 GWELKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPLGLQEVAS 120 Query: 121 FVTKRSAWKLVLLGADEEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSS 180 FVTKRSAWKLVLLGADEEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSS Sbjct: 121 FVTKRSAWKLVLLGADEEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSS 180 Query: 181 MLGVPAQETAPAKLIVYLQRFRPQDYQRLLEVNSSRERPQETEDPDHCLG 230 MLGVPAQETAPAKLIVYLQRFRPQDYQRLLEVNSSRERPQETEDPDHCLG Sbjct: 181 MLGVPAQETAPAKLIVYLQRFRPQDYQRLLEVNSSRERPQETEDPDHCLG 230 >5A65-A nrPDB Length = 217 Score = 387 bits (994), Expect = e-108, Method: Composition-based stats. Identities = 160/215 (74%), Positives = 177/215 (82%) Query: 1 MAQGLIEVERKFLPGPGTEERLQELGGTLEYRVTFRDTYYDTPELSLMQADHWLRRREDS 60 M QGLIEVERKF PGP TEERLQELG TLE+RVTFRDTYYDT ELSLM +DHWLR+RE S Sbjct: 3 MTQGLIEVERKFAPGPDTEERLQELGATLEHRVTFRDTYYDTSELSLMLSDHWLRQREGS 62 Query: 61 GWELKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPLGLQEVAS 120 GWELKCPG GV GPH EY E+T+E IVAQL ++L + VAAVLG L LQEVAS Sbjct: 63 GWELKCPGVTGVSGPHNEYVEVTSEAAIVAQLFELLGSGEQKPAGVAAVLGSLKLQEVAS 122 Query: 121 FVTKRSAWKLVLLGADEEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSS 180 F+T RS+WKL L GA +EPQL +DLD+ADFGYAVGEVEA+VHE+AEVP ALEKI +SS Sbjct: 123 FITTRSSWKLALSGAHGQEPQLTIDLDSADFGYAVGEVEAMVHEKAEVPAALEKIITVSS 182 Query: 181 MLGVPAQETAPAKLIVYLQRFRPQDYQRLLEVNSS 215 MLGVPAQE APAKL+VYLQRFRP DYQRLLE SS Sbjct: 183 MLGVPAQEEAPAKLMVYLQRFRPLDYQRLLEAASS 217 >5A60-A nrPDB Length = 435 Score = 39.2 bits (90), Expect = 0.001, Method: Composition-based stats. Identities = 40/122 (32%), Positives = 58/122 (47%), Gaps = 12/122 (9%) Query: 1 MAQGLIEVERKFLPGPGTEERLQE----LGGTLEYRVTFRDTYYDTPELSLMQADHWLRR 56 MAQ E+E KF+ E L++ LGG V + YY+TP+ L D LR Sbjct: 3 MAQ---EIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETPDNWLRGHDMGLRI 59 Query: 57 REDSG---WELKCPG-AAGVLGPHTEYKELTAEPTI-VAQLCKVLRADGLGAGDVAAVLG 111 R ++G +K G G L EY +EPT+ +AQL + +G D+A+ + Sbjct: 60 RGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEVWPNGELPADLASRVQ 119 Query: 112 PL 113 PL Sbjct: 120 PL 121 >1TP7-A nrPDB Length = 460 Score = 29.6 bits (65), Expect = 1.2, Method: Composition-based stats. Identities = 13/35 (37%), Positives = 21/35 (60%) Query: 137 EEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTA 171 E++P+L+VD D+A F G E ++E +V A Sbjct: 45 EKDPRLKVDFDSALFSKYKGNTECSLNEHIQVAVA 79 >4K2H-A nrPDB Length = 188 Score = 28.5 bits (62), Expect = 2.4, Method: Composition-based stats. Identities = 31/107 (28%), Positives = 45/107 (42%), Gaps = 14/107 (13%) Query: 38 TYYDTPELSLMQADHWLRRREDSGWE-LKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVL 96 +Y+D P M AD L R+ + ++ + PG GP L A P ++A + + Sbjct: 47 SYHDIP----MVADSTLSERQQALFDAVVLPG-----GPQGS-ANLAANPAVIAFVARHD 96 Query: 97 RADGLG---AGDVAAVLGPLGLQEVASFVTKRSAWKLVLLGADEEEP 140 A L A A VLG GL + +V WK V G + P Sbjct: 97 AAGKLICPIASAAARVLGAHGLLKGRRYVCSGDLWKAVPEGVYVDAP 143 >2RPI-A nrPDB Length = 112 Score = 28.5 bits (62), Expect = 2.6, Method: Composition-based stats. Identities = 16/54 (29%), Positives = 20/54 (37%), Gaps = 5/54 (9%) Query: 14 PGPGTEERLQELGGTLEYRVTFR-----DTYYDTPELSLMQADHWLRRREDSGW 62 PGPGT EL +E + D Y D+ L + WL GW Sbjct: 21 PGPGTTNNRMELKAAIEGLKALKEPAEVDLYTDSHYLKKAFTEGWLEGWRKRGW 74 >3IP1-A nrPDB Length = 404 Score = 28.1 bits (61), Expect = 3.1, Method: Composition-based stats. Identities = 22/64 (34%), Positives = 31/64 (48%), Gaps = 3/64 (4%) Query: 85 EPTIVAQLCKVLRADGLGAGDVAAVL--GPLGLQEVASFVTKRSAWKLVLLGADEEEPQL 142 EPT VA ++R G+ GD +L GP+GL VA + A K++L E L Sbjct: 195 EPTSVAYNAVIVRGGGIRPGDNVVILGGGPIGLAAVA-ILKHAGASKVILSEPSEVRRNL 253 Query: 143 RVDL 146 +L Sbjct: 254 AKEL 257 >5VVI-A nrPDB Length = 210 Score = 26.5 bits (57), Expect = 9.7, Method: Composition-based stats. Identities = 26/99 (26%), Positives = 43/99 (43%), Gaps = 4/99 (4%) Query: 101 LGAGDVAAVLGPLGLQEVASFVTKRSAWKLVLLGADEEEPQLRVDLDTADFGYAVG--EV 158 L + A+ L + +A F+ R ++ L+G V AD GYA G E Sbjct: 5 LRIAAMPALANGLLPRFLAQFIRDRPNLQVSLMGLPSSMVMEAVASGRADIGYADGPQER 64 Query: 159 EALVHEEAEVPT--ALEKIHRLSSMLGVPAQETAPAKLI 195 + + E +P A+ HRL+ + V Q+ A ++I Sbjct: 65 QGFLIETRSLPAVVAVPMGHRLAGLDRVTPQDLAGERII 103 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.312 0.137 0.400 Lambda K H 0.267 0.0399 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,115,082 Number of Sequences: 36641 Number of extensions: 358182 Number of successful extensions: 985 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 975 Number of HSP's gapped (non-prelim): 24 length of query: 230 length of database: 10,336,785 effective HSP length: 94 effective length of query: 136 effective length of database: 6,892,531 effective search space: 937384216 effective search space used: 937384216 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.3 bits) S2: 57 (26.6 bits)