BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9BSB4 (218 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5XV4-B nrPDB 447 e-126 4YK8-A nrPDB 50 8e-07 3PTM-A nrPDB 30 0.67 3CI0-J nrPDB 29 1.3 4Y97-B nrPDB 29 1.9 5UGH-A nrPDB 28 4.4 2W42-A nrPDB 27 4.6 6ID1-L nrPDB 27 5.0 5G2R-A nrPDB 27 9.2 >5XV4-B nrPDB Length = 218 Score = 447 bits (1151), Expect = e-126, Method: Compositional matrix adjust. Identities = 215/218 (98%), Positives = 215/218 (98%) Query: 1 MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFI 60 MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHY GTYSIGTVGTQDVDCDFI Sbjct: 1 MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYAAAGTYSIGTVGTQDVDCDFI 60 Query: 61 DFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWE 120 DFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWE Sbjct: 61 DFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWE 120 Query: 121 VWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDT 180 VWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDT Sbjct: 121 VWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDT 180 Query: 181 GLRDVQPYLYKISFQITDALGTSVTTTMRRLIKDTLAL 218 GLRDVQPYLYKISFQITDALGTSVTTTMRRLIKDTLAL Sbjct: 181 GLRDVQPYLYKISFQITDALGTSVTTTMRRLIKDTLAL 218 >4YK8-A nrPDB Length = 187 Score = 50.1 bits (118), Expect = 8e-07, Method: Compositional matrix adjust. Identities = 42/167 (25%), Positives = 67/167 (40%), Gaps = 23/167 (13%) Query: 8 LEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFIDFTYVRV 67 +E+ + + E + AVL +L HR TV + +D +D T + Sbjct: 10 IELKIGYKYAAEVVKAVLGVILFHRQ-------------FSTVPARTIDV--LDITVPTL 54 Query: 68 SSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKK-KSRWPFSDECIPWEVWTVKV 126 EL+ L EF D +RN G GQM L Y++ K W IPWE W + Sbjct: 55 VGAELNEQLATKAAEFIDTIRNEAGAN-GQMILLLYERSPKKSWFGKGNTIPWEQWILHT 113 Query: 127 HVVALA-TEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQS 172 ++ + QE + E E++ + + + YLP + S Sbjct: 114 TILEEGDSYQESSLSLEAAVEQIVQAV-----NLRSLSYLPPVAMDS 155 >3PTM-A nrPDB Length = 505 Score = 30.4 bits (67), Expect = 0.67, Method: Compositional matrix adjust. Identities = 19/74 (25%), Positives = 31/74 (41%) Query: 14 GRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFIDFTYVRVSSEELD 73 G E A H +L H T + + K G +G V F+ F+ + +++ Sbjct: 228 GDSGREPYTACHHQLLAHAETVRLYKAKYQALQKGKIGITLVSHWFVPFSRSKSNNDAAK 287 Query: 74 RALRKVVGEFKDAL 87 RA+ + G F D L Sbjct: 288 RAIDFMFGWFMDPL 301 >3CI0-J nrPDB Length = 163 Score = 29.3 bits (64), Expect = 1.3, Method: Compositional matrix adjust. Identities = 18/45 (40%), Positives = 27/45 (60%), Gaps = 2/45 (4%) Query: 175 DNVFDTGLRDVQPYLYKISFQITDALGTSVTTTMRRLIK-DTLAL 218 N+ G R YL ++++ +TDA G SV TM++LI D+L L Sbjct: 76 SNLLTVGYRIHDGYLERLAWPLTDAAG-SVKPTMQKLIPADSLRL 119 >4Y97-B nrPDB Length = 180 Score = 28.9 bits (63), Expect = 1.9, Method: Compositional matrix adjust. Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 8/56 (14%) Query: 166 PKMPTQSEVDNVFDTGLRDVQPYLYKIS--------FQITDALGTSVTTTMRRLIK 213 P T++ DNVFD D++P LY+ S T L + +RR IK Sbjct: 20 PTCGTENIYDNVFDGSGTDMEPSLYRCSNIDCKASPLTFTVQLSNKLIMDIRRFIK 75 >5UGH-A nrPDB Length = 397 Score = 27.7 bits (60), Expect = 4.4, Method: Compositional matrix adjust. Identities = 9/36 (25%), Positives = 23/36 (63%) Query: 121 VWTVKVHVVALATEQERQICREKVGEKLCEKIINIV 156 V ++VH + ++ + + ++C +++ + L EK+I V Sbjct: 197 VLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAV 232 >2W42-A nrPDB Length = 427 Score = 27.3 bits (59), Expect = 4.6, Method: Compositional matrix adjust. Identities = 21/99 (21%), Positives = 46/99 (46%), Gaps = 14/99 (14%) Query: 118 PWEVWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEV--MNRHEYLPKMP----TQ 171 P+EV ++K + +AL + ++ ++ KI + + +N E++ P + Sbjct: 38 PYEVPSLKYNQIALIHNNQFSSLINQLKSQISSKIDEVWHIHNINISEFIYDSPHFDSIK 97 Query: 172 SEVDNVFDTGLRDVQ--------PYLYKISFQITDALGT 202 S+VDN DTG+ + P YK+ + +++ + Sbjct: 98 SQVDNAIDTGVDGIMLVLPEYNTPLYYKLKSYLINSIPS 136 >6ID1-L nrPDB Length = 802 Score = 27.3 bits (59), Expect = 5.0, Method: Compositional matrix adjust. Identities = 24/90 (26%), Positives = 42/90 (46%), Gaps = 6/90 (6%) Query: 17 VEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFIDFT-YVRVSSEELDRA 75 +++ M+ +LH LLH KK T GT ++ + +++ Y + S EEL +A Sbjct: 575 IKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHI--TYLEHNPYEKFSKEELKKA 632 Query: 76 LRKVVGEFKDALRNSGGDGLGQMSLEFYQK 105 +V E + G G++S E Y + Sbjct: 633 QDVLVQEMEVV---KQGMSHGELSSEAYNQ 659 >5G2R-A nrPDB Length = 471 Score = 26.6 bits (57), Expect = 9.2, Method: Compositional matrix adjust. Identities = 13/40 (32%), Positives = 20/40 (50%) Query: 57 CDFIDFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLG 96 C +D VR +EL++ L + V D + SGG +G Sbjct: 237 CKVVDLGIVRDDRKELEKVLDEAVSSGVDIILTSGGVSMG 276 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5XV4-B nrPDB 386 e-108 4YK8-A nrPDB 192 8e-50 Sequences not found previously or not previously below threshold: 3I42-A nrPDB 31 0.36 4Y97-B nrPDB 30 0.55 3CI0-J nrPDB 29 1.6 5OQL-c nrPDB 28 3.6 6BT2-A nrPDB 28 3.8 2W42-A nrPDB 27 4.4 CONVERGED! >5XV4-B nrPDB Length = 218 Score = 386 bits (992), Expect = e-108, Method: Composition-based stats. Identities = 215/218 (98%), Positives = 215/218 (98%) Query: 1 MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFI 60 MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHY GTYSIGTVGTQDVDCDFI Sbjct: 1 MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYAAAGTYSIGTVGTQDVDCDFI 60 Query: 61 DFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWE 120 DFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWE Sbjct: 61 DFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWE 120 Query: 121 VWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDT 180 VWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDT Sbjct: 121 VWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDT 180 Query: 181 GLRDVQPYLYKISFQITDALGTSVTTTMRRLIKDTLAL 218 GLRDVQPYLYKISFQITDALGTSVTTTMRRLIKDTLAL Sbjct: 181 GLRDVQPYLYKISFQITDALGTSVTTTMRRLIKDTLAL 218 >4YK8-A nrPDB Length = 187 Score = 192 bits (488), Expect = 8e-50, Method: Composition-based stats. Identities = 42/167 (25%), Positives = 67/167 (40%), Gaps = 23/167 (13%) Query: 8 LEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFIDFTYVRV 67 +E+ + + E + AVL +L HR TV + +D +D T + Sbjct: 10 IELKIGYKYAAEVVKAVLGVILFHRQ-------------FSTVPARTIDV--LDITVPTL 54 Query: 68 SSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKK-KSRWPFSDECIPWEVWTVKV 126 EL+ L EF D +RN G GQM L Y++ K W IPWE W + Sbjct: 55 VGAELNEQLATKAAEFIDTIRNEAGAN-GQMILLLYERSPKKSWFGKGNTIPWEQWILHT 113 Query: 127 HVVALA-TEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQS 172 ++ + QE + E E++ + + + YLP + S Sbjct: 114 TILEEGDSYQESSLSLEAAVEQIVQAV-----NLRSLSYLPPVAMDS 155 >3I42-A nrPDB Length = 127 Score = 31.2 bits (69), Expect = 0.36, Method: Composition-based stats. Identities = 19/96 (19%), Positives = 36/96 (37%), Gaps = 10/96 (10%) Query: 13 EGRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFIDFTYVRVSSEEL 72 + + E +L + + Y GT ++ + T+ D FID S L Sbjct: 11 DYQAAAETFKELLEMLGF-----QADYVMSGTDALHAMSTRGYDAVFIDLNLPDTSGLAL 65 Query: 73 DRALR----KVVGEFKDALRNSGGDGLGQMSLEFYQ 104 + LR + +F A+ + LG+ + E + Sbjct: 66 VKQLRALPMEKTSKFV-AVSGFAKNDLGKEACELFD 100 >4Y97-B nrPDB Length = 180 Score = 30.5 bits (67), Expect = 0.55, Method: Composition-based stats. Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 8/56 (14%) Query: 166 PKMPTQSEVDNVFDTGLRDVQPYLYKIS--------FQITDALGTSVTTTMRRLIK 213 P T++ DNVFD D++P LY+ S T L + +RR IK Sbjct: 20 PTCGTENIYDNVFDGSGTDMEPSLYRCSNIDCKASPLTFTVQLSNKLIMDIRRFIK 75 >3CI0-J nrPDB Length = 163 Score = 28.9 bits (63), Expect = 1.6, Method: Composition-based stats. Identities = 18/44 (40%), Positives = 27/44 (61%), Gaps = 2/44 (4%) Query: 176 NVFDTGLRDVQPYLYKISFQITDALGTSVTTTMRRLI-KDTLAL 218 N+ G R YL ++++ +TDA G SV TM++LI D+L L Sbjct: 77 NLLTVGYRIHDGYLERLAWPLTDAAG-SVKPTMQKLIPADSLRL 119 >5OQL-c nrPDB Length = 785 Score = 27.8 bits (60), Expect = 3.6, Method: Composition-based stats. Identities = 35/188 (18%), Positives = 77/188 (40%), Gaps = 22/188 (11%) Query: 16 QVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFIDFTYVRVSSEELDRA 75 ++ E + + ++ R+ + ++ +D++ D + V +EE+ + Sbjct: 482 KIAEQIRKLEAELVAKRAWTLAGEATAADRPVNSLLGEDMEFDHVGKPVP-VVTEEVSES 540 Query: 76 L------RKVVGEFKDALR-------NSGGDGLGQMSLEFYQKKKSRWPFSDECIPWEVW 122 + R + GEF + LR N G G + +E + K+S I E Sbjct: 541 IEELIKRRILAGEFDEVLRRRPDMFGNPHGVRRGLVDVEDTKAKQSL-----AEIYEEEA 595 Query: 123 TVKVHVVALATEQERQICR-EKVGEKLCEKIINIVEVMNRHEYLP--KMPTQSEVDNVFD 179 K + A + + ++ R E+ +++ ++I ++ ++ Y P PT + V +V Sbjct: 596 VKKANPDAYVSAADEKLRRDEEEIKRMWKEISAKLDALSSWHYKPKPPAPTLTVVSDVAT 655 Query: 180 TGLRDVQP 187 + D QP Sbjct: 656 VAMEDAQP 663 >6BT2-A nrPDB Length = 313 Score = 27.8 bits (60), Expect = 3.8, Method: Composition-based stats. Identities = 20/77 (25%), Positives = 32/77 (41%), Gaps = 8/77 (10%) Query: 121 VWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDT 180 W + + + Q E + K E+ I+E + Y P + EVD+ FDT Sbjct: 29 QWNILAQALGEGKDNFVQCPVEAL--KWEERKCLILEEI--LAYQPDILCLQEVDHYFDT 84 Query: 181 GLRDVQPYLYKISFQIT 197 QP L ++ +Q T Sbjct: 85 ----FQPLLSRLGYQGT 97 >2W42-A nrPDB Length = 427 Score = 27.4 bits (59), Expect = 4.4, Method: Composition-based stats. Identities = 21/97 (21%), Positives = 45/97 (46%), Gaps = 14/97 (14%) Query: 118 PWEVWTVKVHVVALATEQERQICREKVGEKLCEKIINI--VEVMNRHEYLPKMP----TQ 171 P+EV ++K + +AL + ++ ++ KI + + +N E++ P + Sbjct: 38 PYEVPSLKYNQIALIHNNQFSSLINQLKSQISSKIDEVWHIHNINISEFIYDSPHFDSIK 97 Query: 172 SEVDNVFDTGLRDVQ--------PYLYKISFQITDAL 200 S+VDN DTG+ + P YK+ + +++ Sbjct: 98 SQVDNAIDTGVDGIMLVLPEYNTPLYYKLKSYLINSI 134 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.314 0.128 0.355 Lambda K H 0.267 0.0396 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,752,886 Number of Sequences: 36641 Number of extensions: 290380 Number of successful extensions: 761 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 746 Number of HSP's gapped (non-prelim): 20 length of query: 218 length of database: 10,336,785 effective HSP length: 93 effective length of query: 125 effective length of database: 6,929,172 effective search space: 866146500 effective search space used: 866146500 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 57 (26.6 bits)