BLASTP 2.2.23 [Feb-03-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= Q9BSB4
(218 letters)
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
36,641 sequences; 10,336,785 total letters
Searching..................................................done
Results from round 1
Score E
Sequences producing significant alignments: (bits) Value
5XV4-B nrPDB 447 e-126
4YK8-A nrPDB 50 8e-07
3PTM-A nrPDB 30 0.67
3CI0-J nrPDB 29 1.3
4Y97-B nrPDB 29 1.9
5UGH-A nrPDB 28 4.4
2W42-A nrPDB 27 4.6
6ID1-L nrPDB 27 5.0
5G2R-A nrPDB 27 9.2
>5XV4-B nrPDB
Length = 218
Score = 447 bits (1151), Expect = e-126, Method: Compositional matrix adjust.
Identities = 215/218 (98%), Positives = 215/218 (98%)
Query: 1 MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFI 60
MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHY GTYSIGTVGTQDVDCDFI
Sbjct: 1 MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYAAAGTYSIGTVGTQDVDCDFI 60
Query: 61 DFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWE 120
DFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWE
Sbjct: 61 DFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWE 120
Query: 121 VWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDT 180
VWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDT
Sbjct: 121 VWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDT 180
Query: 181 GLRDVQPYLYKISFQITDALGTSVTTTMRRLIKDTLAL 218
GLRDVQPYLYKISFQITDALGTSVTTTMRRLIKDTLAL
Sbjct: 181 GLRDVQPYLYKISFQITDALGTSVTTTMRRLIKDTLAL 218
>4YK8-A nrPDB
Length = 187
Score = 50.1 bits (118), Expect = 8e-07, Method: Compositional matrix adjust.
Identities = 42/167 (25%), Positives = 67/167 (40%), Gaps = 23/167 (13%)
Query: 8 LEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFIDFTYVRV 67
+E+ + + E + AVL +L HR TV + +D +D T +
Sbjct: 10 IELKIGYKYAAEVVKAVLGVILFHRQ-------------FSTVPARTIDV--LDITVPTL 54
Query: 68 SSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKK-KSRWPFSDECIPWEVWTVKV 126
EL+ L EF D +RN G GQM L Y++ K W IPWE W +
Sbjct: 55 VGAELNEQLATKAAEFIDTIRNEAGAN-GQMILLLYERSPKKSWFGKGNTIPWEQWILHT 113
Query: 127 HVVALA-TEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQS 172
++ + QE + E E++ + + + YLP + S
Sbjct: 114 TILEEGDSYQESSLSLEAAVEQIVQAV-----NLRSLSYLPPVAMDS 155
>3PTM-A nrPDB
Length = 505
Score = 30.4 bits (67), Expect = 0.67, Method: Compositional matrix adjust.
Identities = 19/74 (25%), Positives = 31/74 (41%)
Query: 14 GRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFIDFTYVRVSSEELD 73
G E A H +L H T + + K G +G V F+ F+ + +++
Sbjct: 228 GDSGREPYTACHHQLLAHAETVRLYKAKYQALQKGKIGITLVSHWFVPFSRSKSNNDAAK 287
Query: 74 RALRKVVGEFKDAL 87
RA+ + G F D L
Sbjct: 288 RAIDFMFGWFMDPL 301
>3CI0-J nrPDB
Length = 163
Score = 29.3 bits (64), Expect = 1.3, Method: Compositional matrix adjust.
Identities = 18/45 (40%), Positives = 27/45 (60%), Gaps = 2/45 (4%)
Query: 175 DNVFDTGLRDVQPYLYKISFQITDALGTSVTTTMRRLIK-DTLAL 218
N+ G R YL ++++ +TDA G SV TM++LI D+L L
Sbjct: 76 SNLLTVGYRIHDGYLERLAWPLTDAAG-SVKPTMQKLIPADSLRL 119
>4Y97-B nrPDB
Length = 180
Score = 28.9 bits (63), Expect = 1.9, Method: Compositional matrix adjust.
Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 8/56 (14%)
Query: 166 PKMPTQSEVDNVFDTGLRDVQPYLYKIS--------FQITDALGTSVTTTMRRLIK 213
P T++ DNVFD D++P LY+ S T L + +RR IK
Sbjct: 20 PTCGTENIYDNVFDGSGTDMEPSLYRCSNIDCKASPLTFTVQLSNKLIMDIRRFIK 75
>5UGH-A nrPDB
Length = 397
Score = 27.7 bits (60), Expect = 4.4, Method: Compositional matrix adjust.
Identities = 9/36 (25%), Positives = 23/36 (63%)
Query: 121 VWTVKVHVVALATEQERQICREKVGEKLCEKIINIV 156
V ++VH + ++ + + ++C +++ + L EK+I V
Sbjct: 197 VLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAV 232
>2W42-A nrPDB
Length = 427
Score = 27.3 bits (59), Expect = 4.6, Method: Compositional matrix adjust.
Identities = 21/99 (21%), Positives = 46/99 (46%), Gaps = 14/99 (14%)
Query: 118 PWEVWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEV--MNRHEYLPKMP----TQ 171
P+EV ++K + +AL + ++ ++ KI + + +N E++ P +
Sbjct: 38 PYEVPSLKYNQIALIHNNQFSSLINQLKSQISSKIDEVWHIHNINISEFIYDSPHFDSIK 97
Query: 172 SEVDNVFDTGLRDVQ--------PYLYKISFQITDALGT 202
S+VDN DTG+ + P YK+ + +++ +
Sbjct: 98 SQVDNAIDTGVDGIMLVLPEYNTPLYYKLKSYLINSIPS 136
>6ID1-L nrPDB
Length = 802
Score = 27.3 bits (59), Expect = 5.0, Method: Compositional matrix adjust.
Identities = 24/90 (26%), Positives = 42/90 (46%), Gaps = 6/90 (6%)
Query: 17 VEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFIDFT-YVRVSSEELDRA 75
+++ M+ +LH LLH KK T GT ++ + +++ Y + S EEL +A
Sbjct: 575 IKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHI--TYLEHNPYEKFSKEELKKA 632
Query: 76 LRKVVGEFKDALRNSGGDGLGQMSLEFYQK 105
+V E + G G++S E Y +
Sbjct: 633 QDVLVQEMEVV---KQGMSHGELSSEAYNQ 659
>5G2R-A nrPDB
Length = 471
Score = 26.6 bits (57), Expect = 9.2, Method: Compositional matrix adjust.
Identities = 13/40 (32%), Positives = 20/40 (50%)
Query: 57 CDFIDFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLG 96
C +D VR +EL++ L + V D + SGG +G
Sbjct: 237 CKVVDLGIVRDDRKELEKVLDEAVSSGVDIILTSGGVSMG 276
Searching..................................................done
Results from round 2
Score E
Sequences producing significant alignments: (bits) Value
Sequences used in model and found again:
5XV4-B nrPDB 386 e-108
4YK8-A nrPDB 192 8e-50
Sequences not found previously or not previously below threshold:
3I42-A nrPDB 31 0.36
4Y97-B nrPDB 30 0.55
3CI0-J nrPDB 29 1.6
5OQL-c nrPDB 28 3.6
6BT2-A nrPDB 28 3.8
2W42-A nrPDB 27 4.4
CONVERGED!
>5XV4-B nrPDB
Length = 218
Score = 386 bits (992), Expect = e-108, Method: Composition-based stats.
Identities = 215/218 (98%), Positives = 215/218 (98%)
Query: 1 MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFI 60
MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHY GTYSIGTVGTQDVDCDFI
Sbjct: 1 MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYAAAGTYSIGTVGTQDVDCDFI 60
Query: 61 DFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWE 120
DFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWE
Sbjct: 61 DFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWE 120
Query: 121 VWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDT 180
VWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDT
Sbjct: 121 VWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDT 180
Query: 181 GLRDVQPYLYKISFQITDALGTSVTTTMRRLIKDTLAL 218
GLRDVQPYLYKISFQITDALGTSVTTTMRRLIKDTLAL
Sbjct: 181 GLRDVQPYLYKISFQITDALGTSVTTTMRRLIKDTLAL 218
>4YK8-A nrPDB
Length = 187
Score = 192 bits (488), Expect = 8e-50, Method: Composition-based stats.
Identities = 42/167 (25%), Positives = 67/167 (40%), Gaps = 23/167 (13%)
Query: 8 LEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFIDFTYVRV 67
+E+ + + E + AVL +L HR TV + +D +D T +
Sbjct: 10 IELKIGYKYAAEVVKAVLGVILFHRQ-------------FSTVPARTIDV--LDITVPTL 54
Query: 68 SSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKK-KSRWPFSDECIPWEVWTVKV 126
EL+ L EF D +RN G GQM L Y++ K W IPWE W +
Sbjct: 55 VGAELNEQLATKAAEFIDTIRNEAGAN-GQMILLLYERSPKKSWFGKGNTIPWEQWILHT 113
Query: 127 HVVALA-TEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQS 172
++ + QE + E E++ + + + YLP + S
Sbjct: 114 TILEEGDSYQESSLSLEAAVEQIVQAV-----NLRSLSYLPPVAMDS 155
>3I42-A nrPDB
Length = 127
Score = 31.2 bits (69), Expect = 0.36, Method: Composition-based stats.
Identities = 19/96 (19%), Positives = 36/96 (37%), Gaps = 10/96 (10%)
Query: 13 EGRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFIDFTYVRVSSEEL 72
+ + E +L + + Y GT ++ + T+ D FID S L
Sbjct: 11 DYQAAAETFKELLEMLGF-----QADYVMSGTDALHAMSTRGYDAVFIDLNLPDTSGLAL 65
Query: 73 DRALR----KVVGEFKDALRNSGGDGLGQMSLEFYQ 104
+ LR + +F A+ + LG+ + E +
Sbjct: 66 VKQLRALPMEKTSKFV-AVSGFAKNDLGKEACELFD 100
>4Y97-B nrPDB
Length = 180
Score = 30.5 bits (67), Expect = 0.55, Method: Composition-based stats.
Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 8/56 (14%)
Query: 166 PKMPTQSEVDNVFDTGLRDVQPYLYKIS--------FQITDALGTSVTTTMRRLIK 213
P T++ DNVFD D++P LY+ S T L + +RR IK
Sbjct: 20 PTCGTENIYDNVFDGSGTDMEPSLYRCSNIDCKASPLTFTVQLSNKLIMDIRRFIK 75
>3CI0-J nrPDB
Length = 163
Score = 28.9 bits (63), Expect = 1.6, Method: Composition-based stats.
Identities = 18/44 (40%), Positives = 27/44 (61%), Gaps = 2/44 (4%)
Query: 176 NVFDTGLRDVQPYLYKISFQITDALGTSVTTTMRRLI-KDTLAL 218
N+ G R YL ++++ +TDA G SV TM++LI D+L L
Sbjct: 77 NLLTVGYRIHDGYLERLAWPLTDAAG-SVKPTMQKLIPADSLRL 119
>5OQL-c nrPDB
Length = 785
Score = 27.8 bits (60), Expect = 3.6, Method: Composition-based stats.
Identities = 35/188 (18%), Positives = 77/188 (40%), Gaps = 22/188 (11%)
Query: 16 QVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFIDFTYVRVSSEELDRA 75
++ E + + ++ R+ + ++ +D++ D + V +EE+ +
Sbjct: 482 KIAEQIRKLEAELVAKRAWTLAGEATAADRPVNSLLGEDMEFDHVGKPVP-VVTEEVSES 540
Query: 76 L------RKVVGEFKDALR-------NSGGDGLGQMSLEFYQKKKSRWPFSDECIPWEVW 122
+ R + GEF + LR N G G + +E + K+S I E
Sbjct: 541 IEELIKRRILAGEFDEVLRRRPDMFGNPHGVRRGLVDVEDTKAKQSL-----AEIYEEEA 595
Query: 123 TVKVHVVALATEQERQICR-EKVGEKLCEKIINIVEVMNRHEYLP--KMPTQSEVDNVFD 179
K + A + + ++ R E+ +++ ++I ++ ++ Y P PT + V +V
Sbjct: 596 VKKANPDAYVSAADEKLRRDEEEIKRMWKEISAKLDALSSWHYKPKPPAPTLTVVSDVAT 655
Query: 180 TGLRDVQP 187
+ D QP
Sbjct: 656 VAMEDAQP 663
>6BT2-A nrPDB
Length = 313
Score = 27.8 bits (60), Expect = 3.8, Method: Composition-based stats.
Identities = 20/77 (25%), Positives = 32/77 (41%), Gaps = 8/77 (10%)
Query: 121 VWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDT 180
W + + + Q E + K E+ I+E + Y P + EVD+ FDT
Sbjct: 29 QWNILAQALGEGKDNFVQCPVEAL--KWEERKCLILEEI--LAYQPDILCLQEVDHYFDT 84
Query: 181 GLRDVQPYLYKISFQIT 197
QP L ++ +Q T
Sbjct: 85 ----FQPLLSRLGYQGT 97
>2W42-A nrPDB
Length = 427
Score = 27.4 bits (59), Expect = 4.4, Method: Composition-based stats.
Identities = 21/97 (21%), Positives = 45/97 (46%), Gaps = 14/97 (14%)
Query: 118 PWEVWTVKVHVVALATEQERQICREKVGEKLCEKIINI--VEVMNRHEYLPKMP----TQ 171
P+EV ++K + +AL + ++ ++ KI + + +N E++ P +
Sbjct: 38 PYEVPSLKYNQIALIHNNQFSSLINQLKSQISSKIDEVWHIHNINISEFIYDSPHFDSIK 97
Query: 172 SEVDNVFDTGLRDVQ--------PYLYKISFQITDAL 200
S+VDN DTG+ + P YK+ + +++
Sbjct: 98 SQVDNAIDTGVDGIMLVLPEYNTPLYYKLKSYLINSI 134
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
Posted date: Jan 3, 2023 9:05 PM
Number of letters in database: 10,336,785
Number of sequences in database: 36,641
Lambda K H
0.314 0.128 0.355
Lambda K H
0.267 0.0396 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,752,886
Number of Sequences: 36641
Number of extensions: 290380
Number of successful extensions: 761
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 746
Number of HSP's gapped (non-prelim): 20
length of query: 218
length of database: 10,336,785
effective HSP length: 93
effective length of query: 125
effective length of database: 6,929,172
effective search space: 866146500
effective search space used: 866146500
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 57 (26.6 bits)