BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9BRJ7 (211 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3KVH-A nrPDB 421 e-119 2A8R-A nrPDB 189 8e-49 5XXB-D nrPDB 34 0.041 6CL4-A nrPDB 31 0.37 4M1E-A nrPDB 28 2.2 4C0D-A nrPDB 27 7.4 >3KVH-A nrPDB Length = 214 Score = 421 bits (1083), Expect = e-119, Method: Compositional matrix adjust. Identities = 206/206 (100%), Positives = 206/206 (100%) Query: 6 VPELKQISRVEAMRLGPGWSHSCHAMLYAANPGQLFGRIPMRFSVLMQMRFDGLLGFPGG 65 VPELKQISRVEAMRLGPGWSHSCHAMLYAANPGQLFGRIPMRFSVLMQMRFDGLLGFPGG Sbjct: 2 VPELKQISRVEAMRLGPGWSHSCHAMLYAANPGQLFGRIPMRFSVLMQMRFDGLLGFPGG 61 Query: 66 FVDRRFWSLEDGLNRVLGLGLGCLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQLHAV 125 FVDRRFWSLEDGLNRVLGLGLGCLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQLHAV Sbjct: 62 FVDRRFWSLEDGLNRVLGLGLGCLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQLHAV 121 Query: 126 EISAVHSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNMMPE 185 EISAVHSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNMMPE Sbjct: 122 EISAVHSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNMMPE 181 Query: 186 EKLVEALAAATEKQKKALEKLLPASS 211 EKLVEALAAATEKQKKALEKLLPASS Sbjct: 182 EKLVEALAAATEKQKKALEKLLPASS 207 >2A8R-A nrPDB Length = 212 Score = 189 bits (480), Expect = 8e-49, Method: Compositional matrix adjust. Identities = 96/193 (49%), Positives = 135/193 (69%), Gaps = 5/193 (2%) Query: 7 PELKQISRVEAMRLGPGWSHSCHAMLYAANPGQLFGRIPMRFSVLMQMRFDGLLGFPGGF 66 P + ISR E+++L G+ H+CHA+L+A + +LF R+P+R +LM MRFDG LGFPGGF Sbjct: 16 PRPRNISREESLQL-EGYKHACHALLHAPSQAKLFDRVPIRRVLLMMMRFDGRLGFPGGF 74 Query: 67 VDRRFWSLEDGLNRVL----GLGLGCLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQL 122 VD R SLE+GL R L G L + +TE DY SS + E P + V H Y ++L LE++ Sbjct: 75 VDTRDISLEEGLKRELEEELGPALATVEVTEDDYRSSQVREHPQKCVTHFYIKELKLEEI 134 Query: 123 HAVEISAVHSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNM 182 +E AV+++DHGLEV+GL+RVPLYT +DRVGG P FL N F+ +K QLL+AL+ L + Sbjct: 135 ERIEAEAVNAKDHGLEVMGLIRVPLYTLRDRVGGLPAFLCNNFIGNSKSQLLYALRSLKL 194 Query: 183 MPEEKLVEALAAA 195 + E+++ E L A+ Sbjct: 195 LREDQIQEVLKAS 207 >5XXB-D nrPDB Length = 310 Score = 34.3 bits (77), Expect = 0.041, Method: Compositional matrix adjust. Identities = 28/123 (22%), Positives = 51/123 (41%), Gaps = 13/123 (10%) Query: 80 RVLGLGLGCLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQLHAVEISAVHSRDHGLEV 139 R+LGL + ADY+ E P + A Q ++ A +I A++ H Sbjct: 201 RILGLHV-------ADYMRQLKEEDPEKYSAQF--SQYIKNKIEADDIEAMYKNAHAQ-- 249 Query: 140 LGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNMMPEEKLVEALAAATEKQ 199 + + P +K+R N + TAK Q + +K+ E++ + +A E+ Sbjct: 250 --IRKNPDAVKKERAANVQNVRQGNVIKTAKGQYVRHVKLTKAQRRERVQQKIAMIAEQM 307 Query: 200 KKA 202 +A Sbjct: 308 AEA 310 >6CL4-A nrPDB Length = 313 Score = 31.2 bits (69), Expect = 0.37, Method: Compositional matrix adjust. Identities = 28/82 (34%), Positives = 41/82 (50%), Gaps = 7/82 (8%) Query: 127 ISAVHSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNMMPEE 186 +++V+ +HG EV LVR+ L G P ++NA +S A QLL AL +P+ Sbjct: 123 VTSVNGVNHGSEVADLVRLALTP-----GRLPESIANAAMS-AFGQLLSALAGSPRLPQS 176 Query: 187 KLVEALAAATEKQKKALEKLLP 208 +EAL A T + A P Sbjct: 177 G-IEALEALTSEGVAAFNNKYP 197 >4M1E-A nrPDB Length = 297 Score = 28.5 bits (62), Expect = 2.2, Method: Compositional matrix adjust. Identities = 30/126 (23%), Positives = 49/126 (38%), Gaps = 7/126 (5%) Query: 70 RFWSLEDGLNRVLGLGLGCLRLTEADYLSSHLTEGPHRVVAHL--YARQLTLEQLHAVEI 127 + W E + +LG GLG L ++ ++ PH + + +A +L +L + I Sbjct: 39 QLWQGEPAVGMILGTGLGGLAEQIEQDIAIPYSDIPHFPTSTVKSHAGRLVCGRLRGIPI 98 Query: 128 SAVHSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQL-----LFALKVLNM 182 A+ R H E L +V + + G L QL L +N+ Sbjct: 99 VAMEGRFHYYEGYSLEQVTFPVRVMKAMGVKTLLVTNAAGGINPQLDLSDVLIIEDHINL 158 Query: 183 MPEEKL 188 MPE L Sbjct: 159 MPENPL 164 >4C0D-A nrPDB Length = 812 Score = 26.6 bits (57), Expect = 7.4, Method: Compositional matrix adjust. Identities = 29/103 (28%), Positives = 51/103 (49%), Gaps = 18/103 (17%) Query: 73 SLEDGLNRV-LGLGLGCLRLTEADYLS-SHLTEGPHRVVAHLYARQLTLEQLHAVEISAV 130 S+E+GLN + + + +++ D S +H+TE A L+ TL +++A Sbjct: 190 SMENGLNYMAVAFAMQLVKILLVDERSVAHVTE------ADLFHTIETLMRINA------ 237 Query: 131 HSRDHGLE----VLGLVRVPLYTQKDRVGGFPNFLSNAFVSTA 169 HSR + E ++ +VR DR G PNF+ ++ +S A Sbjct: 238 HSRGNAPEGLPQLMEVVRSNYEAMIDRAHGGPNFMMHSGISQA 280 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3KVH-A nrPDB 360 e-100 2A8R-A nrPDB 296 6e-81 Sequences not found previously or not previously below threshold: 5XXB-D nrPDB 35 0.020 6CL4-A nrPDB 32 0.18 3VIK-A nrPDB 31 0.42 4C0D-A nrPDB 29 1.2 3EB9-A nrPDB 28 2.3 4XGC-C nrPDB 28 3.4 6FYQ-A nrPDB 28 3.4 3GZ5-A nrPDB 28 4.3 5XXU-U nrPDB 27 4.8 3QZ1-A nrPDB 27 4.8 2PNY-A nrPDB 27 6.1 2W45-A nrPDB 27 6.3 3IGZ-B nrPDB 27 6.9 CONVERGED! >3KVH-A nrPDB Length = 214 Score = 360 bits (923), Expect = e-100, Method: Composition-based stats. Identities = 206/206 (100%), Positives = 206/206 (100%) Query: 6 VPELKQISRVEAMRLGPGWSHSCHAMLYAANPGQLFGRIPMRFSVLMQMRFDGLLGFPGG 65 VPELKQISRVEAMRLGPGWSHSCHAMLYAANPGQLFGRIPMRFSVLMQMRFDGLLGFPGG Sbjct: 2 VPELKQISRVEAMRLGPGWSHSCHAMLYAANPGQLFGRIPMRFSVLMQMRFDGLLGFPGG 61 Query: 66 FVDRRFWSLEDGLNRVLGLGLGCLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQLHAV 125 FVDRRFWSLEDGLNRVLGLGLGCLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQLHAV Sbjct: 62 FVDRRFWSLEDGLNRVLGLGLGCLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQLHAV 121 Query: 126 EISAVHSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNMMPE 185 EISAVHSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNMMPE Sbjct: 122 EISAVHSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNMMPE 181 Query: 186 EKLVEALAAATEKQKKALEKLLPASS 211 EKLVEALAAATEKQKKALEKLLPASS Sbjct: 182 EKLVEALAAATEKQKKALEKLLPASS 207 >2A8R-A nrPDB Length = 212 Score = 296 bits (757), Expect = 6e-81, Method: Composition-based stats. Identities = 97/197 (49%), Positives = 136/197 (69%), Gaps = 5/197 (2%) Query: 7 PELKQISRVEAMRLGPGWSHSCHAMLYAANPGQLFGRIPMRFSVLMQMRFDGLLGFPGGF 66 P + ISR E+++L G+ H+CHA+L+A + +LF R+P+R +LM MRFDG LGFPGGF Sbjct: 16 PRPRNISREESLQL-EGYKHACHALLHAPSQAKLFDRVPIRRVLLMMMRFDGRLGFPGGF 74 Query: 67 VDRRFWSLEDGLNRVL----GLGLGCLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQL 122 VD R SLE+GL R L G L + +TE DY SS + E P + V H Y ++L LE++ Sbjct: 75 VDTRDISLEEGLKRELEEELGPALATVEVTEDDYRSSQVREHPQKCVTHFYIKELKLEEI 134 Query: 123 HAVEISAVHSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNM 182 +E AV+++DHGLEV+GL+RVPLYT +DRVGG P FL N F+ +K QLL+AL+ L + Sbjct: 135 ERIEAEAVNAKDHGLEVMGLIRVPLYTLRDRVGGLPAFLCNNFIGNSKSQLLYALRSLKL 194 Query: 183 MPEEKLVEALAAATEKQ 199 + E+++ E L A+ Q Sbjct: 195 LREDQIQEVLKASHRLQ 211 >5XXB-D nrPDB Length = 310 Score = 35.3 bits (80), Expect = 0.020, Method: Composition-based stats. Identities = 27/123 (21%), Positives = 50/123 (40%), Gaps = 13/123 (10%) Query: 80 RVLGLGLGCLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQLHAVEISAVHSRDHGLEV 139 R+LGL + DY+ E P + A Q ++ A +I A++ H Sbjct: 201 RILGLHVA-------DYMRQLKEEDPEKYSAQF--SQYIKNKIEADDIEAMYKNAHA--- 248 Query: 140 LGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNMMPEEKLVEALAAATEKQ 199 + + P +K+R N + TAK Q + +K+ E++ + +A E+ Sbjct: 249 -QIRKNPDAVKKERAANVQNVRQGNVIKTAKGQYVRHVKLTKAQRRERVQQKIAMIAEQM 307 Query: 200 KKA 202 +A Sbjct: 308 AEA 310 >6CL4-A nrPDB Length = 313 Score = 32.3 bits (72), Expect = 0.18, Method: Composition-based stats. Identities = 28/82 (34%), Positives = 41/82 (50%), Gaps = 7/82 (8%) Query: 127 ISAVHSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNMMPEE 186 +++V+ +HG EV LVR+ L G P ++NA +S A QLL AL +P+ Sbjct: 123 VTSVNGVNHGSEVADLVRLALTP-----GRLPESIANAAMS-AFGQLLSALAGSPRLPQS 176 Query: 187 KLVEALAAATEKQKKALEKLLP 208 + EAL A T + A P Sbjct: 177 GI-EALEALTSEGVAAFNNKYP 197 >3VIK-A nrPDB Length = 487 Score = 30.7 bits (68), Expect = 0.42, Method: Composition-based stats. Identities = 26/113 (23%), Positives = 43/113 (38%), Gaps = 18/113 (15%) Query: 72 WSLEDGLNRVLGLGLGCLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQLHAVEIS--- 128 + DG +G+ DYL++H H + HLY ++ EQ V IS Sbjct: 176 LTFMDGYASEIGMAPSINTPGIGDYLAAHTVIHAHARIYHLYDQEFRAEQGGKVGISLNI 235 Query: 129 -----AVHSRDHGLEV-------LGLVRVPLYTQKDRVGGFPNFLSNAFVSTA 169 A +S + LGL P++T++ G +P L + + Sbjct: 236 NWCEPATNSAEDRASCENYQQFNLGLYAHPIFTEE---GDYPAVLKDRVSRNS 285 >4C0D-A nrPDB Length = 812 Score = 29.2 bits (64), Expect = 1.2, Method: Composition-based stats. Identities = 26/101 (25%), Positives = 46/101 (45%), Gaps = 14/101 (13%) Query: 73 SLEDGLN-RVLGLGLGCLRLTEADYLSSHLTEGPHRVVAHLYARQL--TLEQLHAVEISA 129 S+E+GLN + + +++ D S VAH+ L T+E L + + Sbjct: 190 SMENGLNYMAVAFAMQLVKILLVDERS----------VAHVTEADLFHTIETLMRINAHS 239 Query: 130 VHSRDHGL-EVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTA 169 + GL +++ +VR DR G PNF+ ++ +S A Sbjct: 240 RGNAPEGLPQLMEVVRSNYEAMIDRAHGGPNFMMHSGISQA 280 >3EB9-A nrPDB Length = 266 Score = 28.4 bits (62), Expect = 2.3, Method: Composition-based stats. Identities = 16/64 (25%), Positives = 31/64 (48%), Gaps = 2/64 (3%) Query: 3 TAAVPELKQISRVEAMRLGPGWSHSCHAMLYAANPGQLFGRIPMRFSVLMQMRFDGLLG- 61 T+AV + + +AMR+ + ++L + G+ ++P+ VL+ + DG Sbjct: 109 TSAVTPSAEATSADAMRVAEAYGKQLASLLPLKSVGEAGPKVPVFDVVLLGLGSDGHTAS 168 Query: 62 -FPG 64 FPG Sbjct: 169 IFPG 172 >4XGC-C nrPDB Length = 676 Score = 28.0 bits (61), Expect = 3.4, Method: Composition-based stats. Identities = 28/119 (23%), Positives = 54/119 (45%), Gaps = 18/119 (15%) Query: 91 LTEADYLSSHLTEGPHRVVAHLYARQLTLEQLHAVEISAVHSRDHGLEVLGLVRVPLYTQ 150 LT+ DYL L + + H + +LE L + V L R PL Sbjct: 334 LTDDDYLKKKLPQLLRDCLLHFLLFRCSLEFLTEL-------------VGDLPRCPLGKL 380 Query: 151 KDRVGGFPNFLSNAFVSTAKCQLLFALKVLNMMPEEKLVEALAAATEKQKKAL-EKLLP 208 + + + N L+ A +ST + + L++L+ + +++ V + A E+ ++ L E++ P Sbjct: 381 RREL--YVNCLNRAIISTPEYK--ECLQMLSFLSKDEFVAKVNRALERTEQFLVEEIAP 435 >6FYQ-A nrPDB Length = 484 Score = 28.0 bits (61), Expect = 3.4, Method: Composition-based stats. Identities = 17/59 (28%), Positives = 27/59 (45%), Gaps = 6/59 (10%) Query: 3 TAAVPELKQISRVEAMRLGPGWSHSCHAMLYAANPGQLFGRIPMRFSVLMQMRFDGLLG 61 TAA LK I+ ++ RL A+L+ ++ R ++ +RF GLLG Sbjct: 350 TAAAVALKNIAIIKEERLVENSKRMGDALLHG------LKKVKNRLEIVGDVRFVGLLG 402 >3GZ5-A nrPDB Length = 240 Score = 27.6 bits (60), Expect = 4.3, Method: Composition-based stats. Identities = 14/35 (40%), Positives = 17/35 (48%), Gaps = 1/35 (2%) Query: 56 FDGLLGFPGGFVD-RRFWSLEDGLNRVLGLGLGCL 89 F GL G PGGF+D SLE + R L + Sbjct: 49 FLGLWGLPGGFIDETCDESLEQTVLRKLAEKTAVV 83 >5XXU-U nrPDB Length = 233 Score = 27.3 bits (59), Expect = 4.8, Method: Composition-based stats. Identities = 12/47 (25%), Positives = 25/47 (53%), Gaps = 3/47 (6%) Query: 106 HRVVAHLYARQLTLEQLHAVEISAVH-SRDHGLEVLGLVRVPLYTQK 151 HR+ L ++ L + V ++ +++ L+V G VR+P+ T + Sbjct: 132 HRIRITLTSKDLK--SIERVCTELINGAKEKNLQVAGPVRLPVKTLR 176 >3QZ1-A nrPDB Length = 496 Score = 27.3 bits (59), Expect = 4.8, Method: Composition-based stats. Identities = 10/22 (45%), Positives = 14/22 (63%) Query: 74 LEDGLNRVLGLGLGCLRLTEAD 95 L++ L+R LG G C R+T D Sbjct: 316 LQEELDRELGPGASCSRVTYKD 337 >2PNY-A nrPDB Length = 246 Score = 26.9 bits (58), Expect = 6.1, Method: Composition-based stats. Identities = 20/76 (26%), Positives = 31/76 (40%), Gaps = 19/76 (25%) Query: 10 KQISRVEAMRL---------GPGWSHSCH-------AMLYAANPGQLFGRIPMRFSVLMQ 53 +Q+ R+E M + G +CH +L+ A LF + +L+Q Sbjct: 31 RQLQRLEEMLIVVDENDKVIGADTKRNCHLNENIEKGLLHRAFSVVLFN---TKNRILIQ 87 Query: 54 MRFDGLLGFPGGFVDR 69 R D + FPG F D Sbjct: 88 QRSDTKVTFPGYFTDS 103 >2W45-A nrPDB Length = 470 Score = 26.9 bits (58), Expect = 6.3, Method: Composition-based stats. Identities = 11/31 (35%), Positives = 16/31 (51%), Gaps = 2/31 (6%) Query: 57 DGLLGFPGGFVDRRF--WSLEDGLNRVLGLG 85 DG L G ++ + W+L+D R LG G Sbjct: 275 DGRLPSEGDYLLTQDEAWNLKDVRKRKLGPG 305 >3IGZ-B nrPDB Length = 561 Score = 26.9 bits (58), Expect = 6.9, Method: Composition-based stats. Identities = 10/28 (35%), Positives = 14/28 (50%), Gaps = 1/28 (3%) Query: 41 FGRIPMRFSVLM-QMRFDGLLGFPGGFV 67 F R+ + MR+DG LG P F+ Sbjct: 301 FDRVRVPKVRYAGMMRYDGDLGIPNNFL 328 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.141 0.397 Lambda K H 0.267 0.0432 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,439,090 Number of Sequences: 36641 Number of extensions: 353635 Number of successful extensions: 714 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 15 Number of HSP's that attempted gapping in prelim test: 700 Number of HSP's gapped (non-prelim): 26 length of query: 211 length of database: 10,336,785 effective HSP length: 93 effective length of query: 118 effective length of database: 6,929,172 effective search space: 817642296 effective search space used: 817642296 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 57 (26.5 bits)