BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9BQP7 (344 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5ZYT-A nrPDB 676 0.0 5ZYW-A nrPDB 535 e-153 5IPF-A nrPDB 30 1.3 1WVR-A nrPDB 29 2.7 5LL1-A nrPDB 28 4.6 1RC9-A nrPDB 28 4.6 2EPG-A nrPDB 28 7.2 >5ZYT-A nrPDB Length = 324 Score = 676 bits (1745), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/324 (100%), Positives = 324/324 (100%) Query: 21 ESAALVAFSTSSYSCGRKKKVNPYEEVDQEKYSNLVQSVLSSRGVAQTPGSVEEDALLCG 80 ESAALVAFSTSSYSCGRKKKVNPYEEVDQEKYSNLVQSVLSSRGVAQTPGSVEEDALLCG Sbjct: 1 ESAALVAFSTSSYSCGRKKKVNPYEEVDQEKYSNLVQSVLSSRGVAQTPGSVEEDALLCG 60 Query: 81 PVSKHKLPNQGEDRRVPQNWFPIFNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQT 140 PVSKHKLPNQGEDRRVPQNWFPIFNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQT Sbjct: 61 PVSKHKLPNQGEDRRVPQNWFPIFNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQT 120 Query: 141 MTKQQVFLLERWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENL 200 MTKQQVFLLERWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENL Sbjct: 121 MTKQQVFLLERWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENL 180 Query: 201 LKSGYIESVQHILKDVSGVRALESAVQHETLNYIGLLDCVAEYQGKLCVIDWKTSEKPKP 260 LKSGYIESVQHILKDVSGVRALESAVQHETLNYIGLLDCVAEYQGKLCVIDWKTSEKPKP Sbjct: 181 LKSGYIESVQHILKDVSGVRALESAVQHETLNYIGLLDCVAEYQGKLCVIDWKTSEKPKP 240 Query: 261 FIQSTFDNPLQVVAYMGAMNHDTNYSFQVQCGLIVVAYKDGSPAHPHFMDAELCSQYWTK 320 FIQSTFDNPLQVVAYMGAMNHDTNYSFQVQCGLIVVAYKDGSPAHPHFMDAELCSQYWTK Sbjct: 241 FIQSTFDNPLQVVAYMGAMNHDTNYSFQVQCGLIVVAYKDGSPAHPHFMDAELCSQYWTK 300 Query: 321 WLLRLEEYTEKKKNQNIQKPEYSE 344 WLLRLEEYTEKKKNQNIQKPEYSE Sbjct: 301 WLLRLEEYTEKKKNQNIQKPEYSE 324 >5ZYW-A nrPDB Length = 254 Score = 535 bits (1379), Expect = e-153, Method: Compositional matrix adjust. Identities = 254/254 (100%), Positives = 254/254 (100%) Query: 91 GEDRRVPQNWFPIFNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQTMTKQQVFLLE 150 GEDRRVPQNWFPIFNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQTMTKQQVFLLE Sbjct: 1 GEDRRVPQNWFPIFNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQTMTKQQVFLLE 60 Query: 151 RWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENLLKSGYIESVQ 210 RWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENLLKSGYIESVQ Sbjct: 61 RWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENLLKSGYIESVQ 120 Query: 211 HILKDVSGVRALESAVQHETLNYIGLLDCVAEYQGKLCVIDWKTSEKPKPFIQSTFDNPL 270 HILKDVSGVRALESAVQHETLNYIGLLDCVAEYQGKLCVIDWKTSEKPKPFIQSTFDNPL Sbjct: 121 HILKDVSGVRALESAVQHETLNYIGLLDCVAEYQGKLCVIDWKTSEKPKPFIQSTFDNPL 180 Query: 271 QVVAYMGAMNHDTNYSFQVQCGLIVVAYKDGSPAHPHFMDAELCSQYWTKWLLRLEEYTE 330 QVVAYMGAMNHDTNYSFQVQCGLIVVAYKDGSPAHPHFMDAELCSQYWTKWLLRLEEYTE Sbjct: 181 QVVAYMGAMNHDTNYSFQVQCGLIVVAYKDGSPAHPHFMDAELCSQYWTKWLLRLEEYTE 240 Query: 331 KKKNQNIQKPEYSE 344 KKKNQNIQKPEYSE Sbjct: 241 KKKNQNIQKPEYSE 254 >5IPF-A nrPDB Length = 250 Score = 30.4 bits (67), Expect = 1.3, Method: Compositional matrix adjust. Identities = 12/39 (30%), Positives = 21/39 (53%) Query: 162 EDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENL 200 ED F+ + + F R+ E L+ +L P +K+R E + Sbjct: 33 EDSFRGFPTEYFCTSPRYDECLDYVLIPNGMIKDRLEKM 71 >1WVR-A nrPDB Length = 221 Score = 29.3 bits (64), Expect = 2.7, Method: Compositional matrix adjust. Identities = 15/54 (27%), Positives = 25/54 (46%) Query: 104 FNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQTMTKQQVFLLERWKQRMI 157 F+ E KP + + L L+R+V P+ + +L+ + ERW R I Sbjct: 4 FDSESPRKPEIQNEIIDLHNSLRRSVNPTASNMLKMEWYPEAAANAERWAYRCI 57 >5LL1-A nrPDB Length = 298 Score = 28.5 bits (62), Expect = 4.6, Method: Compositional matrix adjust. Identities = 18/85 (21%), Positives = 40/85 (47%), Gaps = 7/85 (8%) Query: 180 HEALESILSPQETLKER-------DENLLKSGYIESVQHILKDVSGVRALESAVQHETLN 232 H +E I + Q TLK R + +++ + +++ H L + G++++ES + Sbjct: 33 HHIIELIANVQLTLKTRKDYLTGDNSDIIPTDTVKNTVHALAKLKGIKSIESFALDICEH 92 Query: 233 YIGLLDCVAEYQGKLCVIDWKTSEK 257 ++ + V + + + WK EK Sbjct: 93 FLTAFNHVTRVKVNIDEVPWKRLEK 117 >1RC9-A nrPDB Length = 221 Score = 28.5 bits (62), Expect = 4.6, Method: Compositional matrix adjust. Identities = 16/54 (29%), Positives = 25/54 (46%) Query: 104 FNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQTMTKQQVFLLERWKQRMI 157 F+ E KP + V L L+R+V P+ + +L+ + ERW R I Sbjct: 4 FDSESPRKPEIQNEIVDLHNSLRRSVNPTASNMLRMEWYPEAADNAERWAYRCI 57 >2EPG-A nrPDB Length = 487 Score = 27.7 bits (60), Expect = 7.2, Method: Compositional matrix adjust. Identities = 20/60 (33%), Positives = 34/60 (56%), Gaps = 6/60 (10%) Query: 19 SVESAALVAFSTSSYSCGRKKKVNPYEEVDQEKYSNLVQSVLSSRGV---AQTPGSVEED 75 E A V+F +S + GRK + ++V +E+ NLV+ L+ RG+ A T +V+E+ Sbjct: 396 GTEKAMEVSFGSSCHGAGRKMSRHQAKKVARER--NLVKE-LAERGILVRAATRATVDEE 452 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5ZYT-A nrPDB 706 0.0 5ZYW-A nrPDB 560 e-160 Sequences not found previously or not previously below threshold: 5IPF-A nrPDB 30 1.1 1WVR-A nrPDB 29 2.4 1RC9-A nrPDB 29 2.7 2HIX-A nrPDB 29 3.2 5LL1-A nrPDB 28 5.4 1CVR-A nrPDB 28 7.9 CONVERGED! >5ZYT-A nrPDB Length = 324 Score = 706 bits (1821), Expect = 0.0, Method: Composition-based stats. Identities = 324/324 (100%), Positives = 324/324 (100%) Query: 21 ESAALVAFSTSSYSCGRKKKVNPYEEVDQEKYSNLVQSVLSSRGVAQTPGSVEEDALLCG 80 ESAALVAFSTSSYSCGRKKKVNPYEEVDQEKYSNLVQSVLSSRGVAQTPGSVEEDALLCG Sbjct: 1 ESAALVAFSTSSYSCGRKKKVNPYEEVDQEKYSNLVQSVLSSRGVAQTPGSVEEDALLCG 60 Query: 81 PVSKHKLPNQGEDRRVPQNWFPIFNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQT 140 PVSKHKLPNQGEDRRVPQNWFPIFNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQT Sbjct: 61 PVSKHKLPNQGEDRRVPQNWFPIFNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQT 120 Query: 141 MTKQQVFLLERWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENL 200 MTKQQVFLLERWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENL Sbjct: 121 MTKQQVFLLERWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENL 180 Query: 201 LKSGYIESVQHILKDVSGVRALESAVQHETLNYIGLLDCVAEYQGKLCVIDWKTSEKPKP 260 LKSGYIESVQHILKDVSGVRALESAVQHETLNYIGLLDCVAEYQGKLCVIDWKTSEKPKP Sbjct: 181 LKSGYIESVQHILKDVSGVRALESAVQHETLNYIGLLDCVAEYQGKLCVIDWKTSEKPKP 240 Query: 261 FIQSTFDNPLQVVAYMGAMNHDTNYSFQVQCGLIVVAYKDGSPAHPHFMDAELCSQYWTK 320 FIQSTFDNPLQVVAYMGAMNHDTNYSFQVQCGLIVVAYKDGSPAHPHFMDAELCSQYWTK Sbjct: 241 FIQSTFDNPLQVVAYMGAMNHDTNYSFQVQCGLIVVAYKDGSPAHPHFMDAELCSQYWTK 300 Query: 321 WLLRLEEYTEKKKNQNIQKPEYSE 344 WLLRLEEYTEKKKNQNIQKPEYSE Sbjct: 301 WLLRLEEYTEKKKNQNIQKPEYSE 324 >5ZYW-A nrPDB Length = 254 Score = 560 bits (1443), Expect = e-160, Method: Composition-based stats. Identities = 254/254 (100%), Positives = 254/254 (100%) Query: 91 GEDRRVPQNWFPIFNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQTMTKQQVFLLE 150 GEDRRVPQNWFPIFNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQTMTKQQVFLLE Sbjct: 1 GEDRRVPQNWFPIFNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQTMTKQQVFLLE 60 Query: 151 RWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENLLKSGYIESVQ 210 RWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENLLKSGYIESVQ Sbjct: 61 RWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENLLKSGYIESVQ 120 Query: 211 HILKDVSGVRALESAVQHETLNYIGLLDCVAEYQGKLCVIDWKTSEKPKPFIQSTFDNPL 270 HILKDVSGVRALESAVQHETLNYIGLLDCVAEYQGKLCVIDWKTSEKPKPFIQSTFDNPL Sbjct: 121 HILKDVSGVRALESAVQHETLNYIGLLDCVAEYQGKLCVIDWKTSEKPKPFIQSTFDNPL 180 Query: 271 QVVAYMGAMNHDTNYSFQVQCGLIVVAYKDGSPAHPHFMDAELCSQYWTKWLLRLEEYTE 330 QVVAYMGAMNHDTNYSFQVQCGLIVVAYKDGSPAHPHFMDAELCSQYWTKWLLRLEEYTE Sbjct: 181 QVVAYMGAMNHDTNYSFQVQCGLIVVAYKDGSPAHPHFMDAELCSQYWTKWLLRLEEYTE 240 Query: 331 KKKNQNIQKPEYSE 344 KKKNQNIQKPEYSE Sbjct: 241 KKKNQNIQKPEYSE 254 >5IPF-A nrPDB Length = 250 Score = 30.4 bits (67), Expect = 1.1, Method: Composition-based stats. Identities = 12/39 (30%), Positives = 21/39 (53%) Query: 162 EDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENL 200 ED F+ + + F R+ E L+ +L P +K+R E + Sbjct: 33 EDSFRGFPTEYFCTSPRYDECLDYVLIPNGMIKDRLEKM 71 >1WVR-A nrPDB Length = 221 Score = 29.3 bits (64), Expect = 2.4, Method: Composition-based stats. Identities = 15/54 (27%), Positives = 25/54 (46%) Query: 104 FNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQTMTKQQVFLLERWKQRMI 157 F+ E KP + + L L+R+V P+ + +L+ + ERW R I Sbjct: 4 FDSESPRKPEIQNEIIDLHNSLRRSVNPTASNMLKMEWYPEAAANAERWAYRCI 57 >1RC9-A nrPDB Length = 221 Score = 29.3 bits (64), Expect = 2.7, Method: Composition-based stats. Identities = 16/54 (29%), Positives = 25/54 (46%) Query: 104 FNPERSDKPNASDPSVPLKIPLQRNVIPSVTRVLQQTMTKQQVFLLERWKQRMI 157 F+ E KP + V L L+R+V P+ + +L+ + ERW R I Sbjct: 4 FDSESPRKPEIQNEIVDLHNSLRRSVNPTASNMLRMEWYPEAADNAERWAYRCI 57 >2HIX-A nrPDB Length = 621 Score = 28.9 bits (63), Expect = 3.2, Method: Composition-based stats. Identities = 19/66 (28%), Positives = 34/66 (51%), Gaps = 5/66 (7%) Query: 148 LLERWKQRMILELGEDGFKEYTSNVFLQGKRFHEALESILSPQETLKERDENLLK-SGYI 206 L+ R ++ I E KEY NVFL ++E ++ P E ++ E+++K + Y+ Sbjct: 347 LMHRKRKSDIYE----AIKEYPVNVFLFDLMYYEDVDYTTKPLEARRKLLESIVKPNDYV 402 Query: 207 ESVQHI 212 + HI Sbjct: 403 KIAHHI 408 >5LL1-A nrPDB Length = 298 Score = 28.1 bits (61), Expect = 5.4, Method: Composition-based stats. Identities = 18/85 (21%), Positives = 40/85 (47%), Gaps = 7/85 (8%) Query: 180 HEALESILSPQETLKER-------DENLLKSGYIESVQHILKDVSGVRALESAVQHETLN 232 H +E I + Q TLK R + +++ + +++ H L + G++++ES + Sbjct: 33 HHIIELIANVQLTLKTRKDYLTGDNSDIIPTDTVKNTVHALAKLKGIKSIESFALDICEH 92 Query: 233 YIGLLDCVAEYQGKLCVIDWKTSEK 257 ++ + V + + + WK EK Sbjct: 93 FLTAFNHVTRVKVNIDEVPWKRLEK 117 >1CVR-A nrPDB Length = 435 Score = 27.7 bits (60), Expect = 7.9, Method: Composition-based stats. Identities = 26/104 (25%), Positives = 40/104 (38%), Gaps = 4/104 (3%) Query: 240 VAEYQGKLCVIDWKTSEKPKPFI-QSTFDNPLQVVAYMGAMNHDTNYSFQVQCGLIVVAY 298 + Y G W TS + Q T N L + + +N D +S ++ A Sbjct: 205 LVNYTGHGSETAWGTSHFGTTHVKQLTNSNQLPFIFDVACVNGDFLFSMPCFAEALMRAQ 264 Query: 299 KDGSPAHPHFMDAELCSQYWTKWLLRLEEYTE---KKKNQNIQK 339 KDG P + A QYW + +E E +K NI++ Sbjct: 265 KDGKPTGTVAIIASTIDQYWAPPMRGQDEMNEILCEKHPNNIKR 308 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.131 0.386 Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,718,085 Number of Sequences: 36641 Number of extensions: 552633 Number of successful extensions: 1250 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 1239 Number of HSP's gapped (non-prelim): 19 length of query: 344 length of database: 10,336,785 effective HSP length: 98 effective length of query: 246 effective length of database: 6,745,967 effective search space: 1659507882 effective search space used: 1659507882 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 59 (27.3 bits)