BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q9BQ15 (211 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4OWW-B nrPDB 430 e-121 4Q4I-A nrPDB 28 2.0 4V7O-A6 nrPDB 28 2.5 4N78-D nrPDB 28 4.1 6G70-A nrPDB 28 4.1 5MIA-A nrPDB 26 9.8 >4OWW-B nrPDB Length = 211 Score = 430 bits (1106), Expect = e-121, Method: Compositional matrix adjust. Identities = 211/211 (100%), Positives = 211/211 (100%) Query: 1 MTTETFVKDIKPGLKNLNLIFIVLETGRVTKTKDGHEVRTCKVADKTGSINISVWDDVGN 60 MTTETFVKDIKPGLKNLNLIFIVLETGRVTKTKDGHEVRTCKVADKTGSINISVWDDVGN Sbjct: 1 MTTETFVKDIKPGLKNLNLIFIVLETGRVTKTKDGHEVRTCKVADKTGSINISVWDDVGN 60 Query: 61 LIQPGDIIRLTKGYASVFKGCLTLYTGRGGDLQKIGEFCMVYSEVPNFSEPNPEYSTQQA 120 LIQPGDIIRLTKGYASVFKGCLTLYTGRGGDLQKIGEFCMVYSEVPNFSEPNPEYSTQQA Sbjct: 61 LIQPGDIIRLTKGYASVFKGCLTLYTGRGGDLQKIGEFCMVYSEVPNFSEPNPEYSTQQA 120 Query: 121 PNKAVQNDSNPSASQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPPHTPSHPPSTRIT 180 PNKAVQNDSNPSASQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPPHTPSHPPSTRIT Sbjct: 121 PNKAVQNDSNPSASQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPPHTPSHPPSTRIT 180 Query: 181 RSQPNHTPAGPPGPSSNPVSNGKETRRSSKR 211 RSQPNHTPAGPPGPSSNPVSNGKETRRSSKR Sbjct: 181 RSQPNHTPAGPPGPSSNPVSNGKETRRSSKR 211 >4Q4I-A nrPDB Length = 891 Score = 28.5 bits (62), Expect = 2.0, Method: Composition-based stats. Identities = 26/118 (22%), Positives = 44/118 (37%), Gaps = 5/118 (4%) Query: 35 GHEVRTCKVADKTGSINISVWDDVGNLIQPGDIIRLTKGYASVFKGCLTLYTGRGGDLQK 94 H +R V + ++V++ +I+ +I G + KG + G Sbjct: 382 AHPIRPDMVIEMNNFYTLTVYEKGAEVIR---MIHTLLGEENFQKGMQLYFERHDGSAAT 438 Query: 95 IGEFCMVYSEVPN--FSEPNPEYSTQQAPNKAVQNDSNPSASQPTTGPSAASPASENQ 150 +F + N S YS P V++D NP Q T S +PA+ +Q Sbjct: 439 CDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTVKDDYNPETEQYTLTISQRTPATPDQ 496 >4V7O-A6 nrPDB Length = 997 Score = 28.5 bits (62), Expect = 2.5, Method: Composition-based stats. Identities = 16/34 (47%), Positives = 18/34 (52%), Gaps = 5/34 (14%) Query: 125 VQNDSNPSASQPTTGPSAASPASENQNGNGLSAP 158 VQN SNPS S PTT A E + +GL P Sbjct: 663 VQNQSNPSISDPTTLLEA-----ERNDPDGLGLP 691 >4N78-D nrPDB Length = 559 Score = 27.7 bits (60), Expect = 4.1, Method: Compositional matrix adjust. Identities = 22/59 (37%), Positives = 25/59 (42%), Gaps = 6/59 (10%) Query: 134 SQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPPHTPSHPPSTRITRSQPNHTPAGPP 192 S P T + A P S GL P GPH P P PPS I S+P P+ P Sbjct: 440 SSPVTVTALAHPPS------GLHPTPSTAPGPHVPLMPPSPPSQVIPASEPKRHPSTLP 492 >6G70-A nrPDB Length = 670 Score = 27.7 bits (60), Expect = 4.1, Method: Composition-based stats. Identities = 16/57 (28%), Positives = 27/57 (47%), Gaps = 7/57 (12%) Query: 112 NPEYSTQQAPNKAVQNDSNPSASQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPP 168 +P++ST+ + + +P AS PSA + EN+ N ++ P G PP Sbjct: 33 HPDFSTEIM--NVTEMEQSPDAS-----PSAHASTEENEMANAVNLPVTEAEGDFPP 82 >5MIA-A nrPDB Length = 546 Score = 26.2 bits (56), Expect = 9.8, Method: Compositional matrix adjust. Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 2/34 (5%) Query: 155 LSAPPGPGGGPHPPHTPSHPPSTRITRSQPNHTP 188 ++ P G GGPHP H H + + +S N TP Sbjct: 405 VALPAGAAGGPHPFHLHGH--NFAVVQSANNATP 436 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4OWW-B nrPDB 404 e-113 Sequences not found previously or not previously below threshold: 4N78-D nrPDB 40 6e-04 1OQY-A nrPDB 32 0.14 5MIA-A nrPDB 30 0.69 4UUD-B nrPDB 30 0.84 4Q4I-A nrPDB 30 0.84 6FEC-u nrPDB 29 2.0 2WNE-A nrPDB 28 3.1 4B6H-C nrPDB 27 4.7 6FML-I nrPDB 27 6.7 6FF4-Y nrPDB 27 7.1 >4OWW-B nrPDB Length = 211 Score = 404 bits (1038), Expect = e-113, Method: Composition-based stats. Identities = 211/211 (100%), Positives = 211/211 (100%) Query: 1 MTTETFVKDIKPGLKNLNLIFIVLETGRVTKTKDGHEVRTCKVADKTGSINISVWDDVGN 60 MTTETFVKDIKPGLKNLNLIFIVLETGRVTKTKDGHEVRTCKVADKTGSINISVWDDVGN Sbjct: 1 MTTETFVKDIKPGLKNLNLIFIVLETGRVTKTKDGHEVRTCKVADKTGSINISVWDDVGN 60 Query: 61 LIQPGDIIRLTKGYASVFKGCLTLYTGRGGDLQKIGEFCMVYSEVPNFSEPNPEYSTQQA 120 LIQPGDIIRLTKGYASVFKGCLTLYTGRGGDLQKIGEFCMVYSEVPNFSEPNPEYSTQQA Sbjct: 61 LIQPGDIIRLTKGYASVFKGCLTLYTGRGGDLQKIGEFCMVYSEVPNFSEPNPEYSTQQA 120 Query: 121 PNKAVQNDSNPSASQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPPHTPSHPPSTRIT 180 PNKAVQNDSNPSASQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPPHTPSHPPSTRIT Sbjct: 121 PNKAVQNDSNPSASQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPPHTPSHPPSTRIT 180 Query: 181 RSQPNHTPAGPPGPSSNPVSNGKETRRSSKR 211 RSQPNHTPAGPPGPSSNPVSNGKETRRSSKR Sbjct: 181 RSQPNHTPAGPPGPSSNPVSNGKETRRSSKR 211 >4N78-D nrPDB Length = 559 Score = 40.1 bits (92), Expect = 6e-04, Method: Composition-based stats. Identities = 22/59 (37%), Positives = 25/59 (42%), Gaps = 6/59 (10%) Query: 134 SQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPPHTPSHPPSTRITRSQPNHTPAGPP 192 S P T + A P S GL P GPH P P PPS I S+P P+ P Sbjct: 440 SSPVTVTALAHPPS------GLHPTPSTAPGPHVPLMPPSPPSQVIPASEPKRHPSTLP 492 >1OQY-A nrPDB Length = 368 Score = 32.4 bits (72), Expect = 0.14, Method: Composition-based stats. Identities = 19/66 (28%), Positives = 31/66 (46%), Gaps = 12/66 (18%) Query: 133 ASQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPPHTPSHPPSTRITRSQPNHTPAGPP 192 A Q T+ P ASP + ++ S PP P G SHPP ++ + +P+ Sbjct: 86 AGQGTSAPPEASPTAAPESST--SFPPAPTSG------MSHPPPA----AREDKSPSEES 133 Query: 193 GPSSNP 198 P+++P Sbjct: 134 APTTSP 139 >5MIA-A nrPDB Length = 546 Score = 30.1 bits (66), Expect = 0.69, Method: Composition-based stats. Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 2/34 (5%) Query: 155 LSAPPGPGGGPHPPHTPSHPPSTRITRSQPNHTP 188 ++ P G GGPHP H H + + +S N TP Sbjct: 405 VALPAGAAGGPHPFHLHGH--NFAVVQSANNATP 436 >4UUD-B nrPDB Length = 864 Score = 29.7 bits (65), Expect = 0.84, Method: Composition-based stats. Identities = 15/44 (34%), Positives = 16/44 (36%), Gaps = 6/44 (13%) Query: 152 GNGLSAPPGPGGGPHPPHTPSHPPSTRITRSQPNHTPAGPPGPS 195 G P PG P P P P S+PN P G P S Sbjct: 810 GGAPPVPSRPGASPDPFGPPPQVP------SRPNRAPPGVPSRS 847 >4Q4I-A nrPDB Length = 891 Score = 29.7 bits (65), Expect = 0.84, Method: Composition-based stats. Identities = 26/118 (22%), Positives = 44/118 (37%), Gaps = 5/118 (4%) Query: 35 GHEVRTCKVADKTGSINISVWDDVGNLIQPGDIIRLTKGYASVFKGCLTLYTGRGGDLQK 94 H +R V + ++V++ +I+ +I G + KG + G Sbjct: 382 AHPIRPDMVIEMNNFYTLTVYEKGAEVIR---MIHTLLGEENFQKGMQLYFERHDGSAAT 438 Query: 95 IGEFCMVYSEVPN--FSEPNPEYSTQQAPNKAVQNDSNPSASQPTTGPSAASPASENQ 150 +F + N S YS P V++D NP Q T S +PA+ +Q Sbjct: 439 CDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTVKDDYNPETEQYTLTISQRTPATPDQ 496 >6FEC-u nrPDB Length = 636 Score = 28.5 bits (62), Expect = 2.0, Method: Composition-based stats. Identities = 30/127 (23%), Positives = 44/127 (34%), Gaps = 33/127 (25%) Query: 106 PNFSEPNPEYSTQQAP------NKAVQNDSNPSA---------SQPTTGPSAASPASENQ 150 P P P+ + P N V+ SNP A PT+G +PA ++ Sbjct: 485 PTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDTEQQSPTSGGGKVAPAQPSE 544 Query: 151 NG---------NGLSAPP--------GPGGGPHPPHTPSHPPSTRITRSQPNHTPAGPPG 193 G +G++AP GPG G + H + Q + + P Sbjct: 545 EGPGRKDENKVDGMNAPKGQTGNSSRGPGDGGNRDHWKESDRKDG-KKDQDSRSAPEPKK 603 Query: 194 PSSNPVS 200 P NP S Sbjct: 604 PEENPAS 610 >2WNE-A nrPDB Length = 298 Score = 28.1 bits (61), Expect = 3.1, Method: Composition-based stats. Identities = 17/56 (30%), Positives = 24/56 (42%), Gaps = 6/56 (10%) Query: 127 NDSNPSASQPTTGPSAASPASENQNGNG------LSAPPGPGGGPHPPHTPSHPPS 176 ND +P+A TG + A PAS G+ ++ G G P S+ PS Sbjct: 123 NDQSPNAMTLHTGANCAMPASRTMTGHATNNNCDVNTDGNTGCGVQAPTANSYGPS 178 >4B6H-C nrPDB Length = 135 Score = 27.4 bits (59), Expect = 4.7, Method: Composition-based stats. Identities = 20/63 (31%), Positives = 28/63 (44%), Gaps = 9/63 (14%) Query: 115 YSTQQAPNKAVQND-------SNPSASQPTTGPSAASPASENQN--GNGLSAPPGPGGGP 165 Y++ A +A+QN +N S + +GP + NQN G S PP P P Sbjct: 63 YNSSAAAWQAMQNGGKNKNFPNNQSWNSSLSGPRLLFKSQANQNYAGAKFSEPPSPSVLP 122 Query: 166 HPP 168 PP Sbjct: 123 KPP 125 >6FML-I nrPDB Length = 219 Score = 27.0 bits (58), Expect = 6.7, Method: Composition-based stats. Identities = 24/101 (23%), Positives = 40/101 (39%), Gaps = 8/101 (7%) Query: 112 NPEYSTQQAPNKAVQNDSNPSASQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPPHTP 171 NP + Q NK ++ S + + SA + + NG G G P + Sbjct: 31 NPNWRPNQRRNKTIKAILGESQRKEASSTSAVATPRADDNGGG-------SGADTPANND 83 Query: 172 SHPP-STRITRSQPNHTPAGPPGPSSNPVSNGKETRRSSKR 211 ++ ST T + N +G P+SN N + RS ++ Sbjct: 84 NNDGLSTSGTSTPANGNGSGAGTPASNGQPNLAQASRSLQK 124 >6FF4-Y nrPDB Length = 904 Score = 26.6 bits (57), Expect = 7.1, Method: Composition-based stats. Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 3/54 (5%) Query: 156 SAPPGPGGGPHPPHTPSHPPSTRITRSQPNHTPAGPPGPSSNPVSNGKETRRSS 209 ++PP P P +PS PP R++ S P + P +P + K + SS Sbjct: 615 ASPPPP---PKRRASPSPPPKRRVSHSPPPKQRSSPVTKRRSPSLSSKHRKGSS 665 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.309 0.130 0.391 Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,826,726 Number of Sequences: 36641 Number of extensions: 439583 Number of successful extensions: 1029 Number of sequences better than 10.0: 31 Number of HSP's better than 10.0 without gapping: 27 Number of HSP's successfully gapped in prelim test: 49 Number of HSP's that attempted gapping in prelim test: 854 Number of HSP's gapped (non-prelim): 168 length of query: 211 length of database: 10,336,785 effective HSP length: 93 effective length of query: 118 effective length of database: 6,929,172 effective search space: 817642296 effective search space used: 817642296 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 57 (26.6 bits)