BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q96PD4 (163 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5N92-F nrPDB 280 3e-76 5N7W-X nrPDB 125 6e-30 5HI3-A nrPDB 125 8e-30 5WH8-A nrPDB 29 1.0 6NLW-A nrPDB 28 1.6 2Y2J-A nrPDB 28 2.1 2JE5-A nrPDB 28 2.1 4NZQ-A nrPDB 27 2.8 5YEI-C nrPDB 27 2.9 2VRK-A nrPDB 26 6.4 2ED8-A nrPDB 26 6.5 >5N92-F nrPDB Length = 139 Score = 280 bits (715), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 133/134 (99%), Positives = 134/134 (100%) Query: 30 ARKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWD 89 +RKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWD Sbjct: 6 SRKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWD 65 Query: 90 PNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVT 149 PNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVT Sbjct: 66 PNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVT 125 Query: 150 VGCTCVTPVIHHVQ 163 VGCTCVTPVIHHVQ Sbjct: 126 VGCTCVTPVIHHVQ 139 >5N7W-X nrPDB Length = 155 Score = 125 bits (315), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 61/108 (56%), Positives = 76/108 (70%) Query: 55 MKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKE 114 + L+I N N S + +RSTSPWN DP RYPS + +A+CR+LGCINA G Sbjct: 47 VNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNV 106 Query: 115 DISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHV 162 D MNSVPIQQE LV+RR+ C SF+LEK+LV+VGCTCVTP++HHV Sbjct: 107 DYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHV 154 >5HI3-A nrPDB Length = 132 Score = 125 bits (314), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 61/108 (56%), Positives = 76/108 (70%) Query: 55 MKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKE 114 + L+I N N S + +RSTSPWN DP RYPS + +A+CR+LGCINA G Sbjct: 24 VNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNV 83 Query: 115 DISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHV 162 D MNSVPIQQE LV+RR+ C SF+LEK+LV+VGCTCVTP++HHV Sbjct: 84 DYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHV 131 >5WH8-A nrPDB Length = 323 Score = 28.9 bits (63), Expect = 1.0, Method: Compositional matrix adjust. Identities = 18/59 (30%), Positives = 31/59 (52%), Gaps = 6/59 (10%) Query: 24 FLSEAAARKIPKVGHTFFQKPESCPPV-PGGSMKLDIGIINEN-----QRVSMSRNIES 76 ++ ARKI V +F KPES + PG S D +++E+ +++S ++ ES Sbjct: 257 WMDWMDARKISWVNWSFADKPESSAALKPGASNSGDWNMVSESGQYIKRKLSQPKSYES 315 >6NLW-A nrPDB Length = 246 Score = 28.1 bits (61), Expect = 1.6, Method: Compositional matrix adjust. Identities = 20/86 (23%), Positives = 37/86 (43%), Gaps = 11/86 (12%) Query: 44 PESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWN----------YTVTWDPNRY 93 P S +P + LD+G++ + +V + ++R + WN Y+V + Sbjct: 49 PASTFKIPNSLIALDLGVVKDEHQV-FKWDGQTRDIAAWNRDHDLITAMKYSVVPVYQEF 107 Query: 94 PSEVVQAQCRNLGCINAQGKEDISMN 119 ++ QA+ + G EDIS N Sbjct: 108 ARQIGQARMSKMLHAFDYGNEDISGN 133 >2Y2J-A nrPDB Length = 494 Score = 27.7 bits (60), Expect = 2.1, Method: Composition-based stats. Identities = 16/53 (30%), Positives = 26/53 (49%), Gaps = 1/53 (1%) Query: 63 NENQRVSMSRNIESRSTSPW-NYTVTWDPNRYPSEVVQAQCRNLGCINAQGKE 114 N N + I+ S S W N DP+ SEV+++ + G ++ +GKE Sbjct: 396 NSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVSVEGKE 448 >2JE5-A nrPDB Length = 720 Score = 27.7 bits (60), Expect = 2.1, Method: Composition-based stats. Identities = 16/53 (30%), Positives = 26/53 (49%), Gaps = 1/53 (1%) Query: 63 NENQRVSMSRNIESRSTSPW-NYTVTWDPNRYPSEVVQAQCRNLGCINAQGKE 114 N N + I+ S S W N DP+ SEV+++ + G ++ +GKE Sbjct: 622 NSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVSVEGKE 674 >4NZQ-A nrPDB Length = 557 Score = 27.3 bits (59), Expect = 2.8, Method: Composition-based stats. Identities = 14/43 (32%), Positives = 20/43 (46%), Gaps = 2/43 (4%) Query: 72 RNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKE 114 RN +S +T PW YT DP E C C+ +G++ Sbjct: 115 RNPDSSTTGPWCYTT--DPTVRRQECSIPVCGQEQCVPDRGQQ 155 >5YEI-C nrPDB Length = 412 Score = 27.3 bits (59), Expect = 2.9, Method: Compositional matrix adjust. Identities = 22/95 (23%), Positives = 41/95 (43%), Gaps = 7/95 (7%) Query: 34 PKVGHTFFQKPES------CPPVPGGSMKLDIGIINENQRVSM-SRNIESRSTSPWNYTV 86 P + F + E+ P PG + K+ I N V M +N+ +T+ + +TV Sbjct: 252 PIISGIAFNRDEAKLTIRGVPDTPGVAFKILGPISAANVEVDMIVQNVAHDNTTDFTFTV 311 Query: 87 TWDPNRYPSEVVQAQCRNLGCINAQGKEDISMNSV 121 + E+++ N+G A G +I+ S+ Sbjct: 312 HRNDYLNALEILKQTAANIGAREAIGDTNIAKVSI 346 >2VRK-A nrPDB Length = 496 Score = 26.2 bits (56), Expect = 6.4, Method: Composition-based stats. Identities = 11/27 (40%), Positives = 14/27 (51%) Query: 37 GHTFFQKPESCPPVPGGSMKLDIGIIN 63 GH F +PE P P KL+ G +N Sbjct: 454 GHNTFDEPERVKPAPFRDFKLEGGHLN 480 >2ED8-A nrPDB Length = 106 Score = 26.2 bits (56), Expect = 6.5, Method: Compositional matrix adjust. Identities = 19/59 (32%), Positives = 32/59 (54%), Gaps = 4/59 (6%) Query: 72 RNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCIN-AQGKE-DISMNSVPIQQETL 128 N+++ STSP + +TW+P Y + VQ L C + GKE +I ++ + + E L Sbjct: 12 ENLQAVSTSPTSILITWEPPAYANGPVQG--YRLFCTEVSTGKEQNIEVDGLSYKLEGL 68 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5N92-F nrPDB 262 4e-71 5N7W-X nrPDB 199 4e-52 5HI3-A nrPDB 198 9e-52 Sequences not found previously or not previously below threshold: 3C9F-A nrPDB 29 0.91 2W6C-X nrPDB 28 1.8 2EEZ-A nrPDB 28 2.2 2NV5-A nrPDB 28 2.5 4BPC-A nrPDB 27 2.7 4DM4-A nrPDB 27 4.6 5FYJ-G nrPDB 27 5.7 2Q4H-A nrPDB 27 5.9 2VRK-A nrPDB 26 6.1 CONVERGED! >5N92-F nrPDB Length = 139 Score = 262 bits (670), Expect = 4e-71, Method: Composition-based stats. Identities = 133/134 (99%), Positives = 134/134 (100%) Query: 30 ARKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWD 89 +RKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWD Sbjct: 6 SRKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWD 65 Query: 90 PNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVT 149 PNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVT Sbjct: 66 PNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVT 125 Query: 150 VGCTCVTPVIHHVQ 163 VGCTCVTPVIHHVQ Sbjct: 126 VGCTCVTPVIHHVQ 139 >5N7W-X nrPDB Length = 155 Score = 199 bits (506), Expect = 4e-52, Method: Composition-based stats. Identities = 61/108 (56%), Positives = 76/108 (70%) Query: 55 MKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKE 114 + L+I N N S + +RSTSPWN DP RYPS + +A+CR+LGCINA G Sbjct: 47 VNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNV 106 Query: 115 DISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHV 162 D MNSVPIQQE LV+RR+ C SF+LEK+LV+VGCTCVTP++HHV Sbjct: 107 DYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHV 154 >5HI3-A nrPDB Length = 132 Score = 198 bits (503), Expect = 9e-52, Method: Composition-based stats. Identities = 61/108 (56%), Positives = 76/108 (70%) Query: 55 MKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKE 114 + L+I N N S + +RSTSPWN DP RYPS + +A+CR+LGCINA G Sbjct: 24 VNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNV 83 Query: 115 DISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHV 162 D MNSVPIQQE LV+RR+ C SF+LEK+LV+VGCTCVTP++HHV Sbjct: 84 DYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHV 131 >3C9F-A nrPDB Length = 557 Score = 29.3 bits (64), Expect = 0.91, Method: Composition-based stats. Identities = 14/53 (26%), Positives = 19/53 (35%) Query: 39 TFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPN 91 T Q C V S+ LD +N R SR+ +T + Y D Sbjct: 252 TGLQSGRYCETVGWTSVNLDKADLNLPVRQRFSRSYIDFNTDSFKYHTNLDKE 304 >2W6C-X nrPDB Length = 586 Score = 28.1 bits (61), Expect = 1.8, Method: Composition-based stats. Identities = 15/63 (23%), Positives = 26/63 (41%), Gaps = 8/63 (12%) Query: 34 PKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRY 93 P VG+ F++PE P G + N + + + ++ + W+PNR Sbjct: 59 PPVGNMRFRRPEPKKPWSG--------VWNASTYPNNCQQYVDEQFPGFSGSEMWNPNRE 110 Query: 94 PSE 96 SE Sbjct: 111 MSE 113 >2EEZ-A nrPDB Length = 369 Score = 27.7 bits (60), Expect = 2.2, Method: Composition-based stats. Identities = 11/24 (45%), Positives = 15/24 (62%) Query: 22 LAFLSEAAARKIPKVGHTFFQKPE 45 L +SE A R P+VG F +KP+ Sbjct: 128 LVPMSEVAGRMAPQVGAQFLEKPK 151 >2NV5-A nrPDB Length = 299 Score = 27.7 bits (60), Expect = 2.5, Method: Composition-based stats. Identities = 17/66 (25%), Positives = 24/66 (36%), Gaps = 6/66 (9%) Query: 46 SCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTS---PW---NYTVTWDPNRYPSEVVQ 99 S PP+P + I + N + S+ ES W N V NRY + + Sbjct: 5 SHPPIPILELADHIERLKANDNLKFSQEYESIDPGQQFTWEHSNLEVNKPKNRYANVIAY 64 Query: 100 AQCRNL 105 R L Sbjct: 65 DHSRVL 70 >4BPC-A nrPDB Length = 582 Score = 27.3 bits (59), Expect = 2.7, Method: Composition-based stats. Identities = 16/64 (25%), Positives = 23/64 (35%), Gaps = 6/64 (9%) Query: 46 SCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTS---PW---NYTVTWDPNRYPSEVVQ 99 S PP+P M + N + +S+ ES W N V NRY + + Sbjct: 3 SHPPIPIADMAEHTERLKANDSLKLSQEYESIDPGQQFTWEHSNLEVNKPKNRYANVIAY 62 Query: 100 AQCR 103 R Sbjct: 63 DHSR 66 >4DM4-A nrPDB Length = 167 Score = 26.9 bits (58), Expect = 4.6, Method: Composition-based stats. Identities = 18/77 (23%), Positives = 30/77 (38%), Gaps = 15/77 (19%) Query: 41 FQKPESCPPVPGGSMKLDIG----------IINENQRVSMSRNIESRSTSPWNYTVTWDP 90 + P + P VP G + ++ II +N R+ R + + TW Sbjct: 30 YVNPRNLPSVPNGLVNIEKNFERISRPIRFIIVDNTRMFTKPEYWDRVVAIFTTGHTWQF 89 Query: 91 NRY----PSEVVQAQCR 103 N Y P E+ Q +C+ Sbjct: 90 NNYQWNSPQELFQ-RCK 105 >5FYJ-G nrPDB Length = 484 Score = 26.6 bits (57), Expect = 5.7, Method: Composition-based stats. Identities = 20/99 (20%), Positives = 35/99 (35%), Gaps = 4/99 (4%) Query: 46 SCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNL 105 +C P ++ + + EN + + +E + WD + P + C L Sbjct: 45 ACVPTDPNPQEIPLKNVTENFNMWKNNMVEQMHEDIISL---WDESLKPCVKLTPLCVTL 101 Query: 106 GCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLE 144 C N S N V T+ R + + CS + E Sbjct: 102 ICTNVTSNSTNSTNGV-TNNSTVDYREQLKNCSFNITTE 139 >2Q4H-A nrPDB Length = 351 Score = 26.6 bits (57), Expect = 5.9, Method: Composition-based stats. Identities = 17/53 (32%), Positives = 24/53 (45%), Gaps = 15/53 (28%) Query: 43 KPESCP--------------PVPGGSMKLDIGIINENQRVSMSRNIESRSTSP 81 KP SCP VP + +I EN ++SRN+E++ST P Sbjct: 59 KPSSCPFCIGREQECAPELFRVPDHDPNWKLRVI-ENLYPALSRNLETQSTQP 110 >2VRK-A nrPDB Length = 496 Score = 26.2 bits (56), Expect = 6.1, Method: Composition-based stats. Identities = 11/32 (34%), Positives = 15/32 (46%) Query: 37 GHTFFQKPESCPPVPGGSMKLDIGIINENQRV 68 GH F +PE P P KL+ G +N + Sbjct: 454 GHNTFDEPERVKPAPFRDFKLEGGHLNASLPP 485 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.310 0.135 0.416 Lambda K H 0.267 0.0413 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,707,932 Number of Sequences: 36641 Number of extensions: 244518 Number of successful extensions: 577 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 21 Number of HSP's that attempted gapping in prelim test: 565 Number of HSP's gapped (non-prelim): 33 length of query: 163 length of database: 10,336,785 effective HSP length: 90 effective length of query: 73 effective length of database: 7,039,095 effective search space: 513853935 effective search space used: 513853935 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.3 bits) S2: 55 (25.8 bits)