BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q96CD2 (204 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1QZU-A nrPDB 417 e-117 1MVN-A nrPDB 177 2e-45 1P3Y-1 nrPDB 59 2e-09 5H75-A nrPDB 59 2e-09 1EB7-A nrPDB 30 0.91 4ZAN-A nrPDB 28 3.4 3NZQ-A nrPDB 28 4.0 5G2V-A nrPDB 27 5.1 >1QZU-A nrPDB Length = 206 Score = 417 bits (1073), Expect = e-117, Method: Compositional matrix adjust. Identities = 203/204 (99%), Positives = 204/204 (100%) Query: 1 MEPKASCPAAAPLMERKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFY 60 MEPKASCPAAAPLMERKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFY Sbjct: 3 MEPKASCPAAAPLMERKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFY 62 Query: 61 SPQDIPVTLYSDADEWEIWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLL 120 SPQDIPVTLYSDADEWE+WKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLL Sbjct: 63 SPQDIPVTLYSDADEWEMWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLL 122 Query: 121 TCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGA 180 TCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGA Sbjct: 123 TCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGA 182 Query: 181 MAEVGTIVDKVKEVLFQHSGFQQS 204 MAEVGTIVDKVKEVLFQHSGFQQS Sbjct: 183 MAEVGTIVDKVKEVLFQHSGFQQS 206 >1MVN-A nrPDB Length = 209 Score = 177 bits (450), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 92/191 (48%), Positives = 119/191 (62%), Gaps = 5/191 (2%) Query: 16 RKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFYSPQDIP--VTLYSDA 73 RK VL+ +GSVAA+K L + EV V T+ + HF +P VTLY+D Sbjct: 18 RKPRVLLAASGSVAAIKFGNLCHCFTEWA--EVRAVVTKSSLHFLDKLSLPQEVTLYTDE 75 Query: 74 DEWEIWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLLTCVMRAWDRSKPL 133 DEW W DPVLHI+LRRWAD+L++APL ANTLGK+A G+CDNLLTC++RAWD +KPL Sbjct: 76 DEWSSWNKIGDPVLHIELRRWADVLVIAPLSANTLGKIAGGLCDNLLTCIIRAWDYTKPL 135 Query: 134 LFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGAMAEVGTIVDKVKE 193 PAMNT MW +P T + + L G IP + K+L GD G GAMAE I V+ Sbjct: 136 FVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLASGDYGNGAMAEPSLIYSTVR- 194 Query: 194 VLFQHSGFQQS 204 + ++ QQ+ Sbjct: 195 LFWESQAHQQT 205 >1P3Y-1 nrPDB Length = 194 Score = 58.5 bits (140), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 25/77 (32%), Positives = 45/77 (58%), Gaps = 2/77 (2%) Query: 88 HIDLRRWADLLLVAPLDANTLGKVASGICDNLLTCVMRAWDRSKPLLFCPAMNTAMWEHP 147 H+++ RWAD+ + P AN LG+ A+G+ NL+ + A + +F P MN MW Sbjct: 75 HVEIGRWADIYCIIPATANILGQTANGVAMNLVATTVLAHPHNT--IFFPNMNDLMWNKT 132 Query: 148 ITAQQVDQLKAFGYVEI 164 + ++ ++QL+ G++ I Sbjct: 133 VVSRNIEQLRKDGHIVI 149 >5H75-A nrPDB Length = 238 Score = 58.5 bits (140), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 26/80 (32%), Positives = 46/80 (57%), Gaps = 2/80 (2%) Query: 88 HIDLRRWADLLLVAPLDANTLGKVASGICDNLLTCVMRAWDRSKPLLFCPAMNTAMWEHP 147 H+++ RWAD+ + P AN LG+ A+G+ NL+ + A + +F P MN MW Sbjct: 82 HVEIGRWADIYCIIPATANILGQTANGVAMNLVATTVLAHPHNT--IFFPNMNDLMWNKT 139 Query: 148 ITAQQVDQLKAFGYVEIPCV 167 + ++ ++QL+ G++ I V Sbjct: 140 VVSRNIEQLRKDGHIVIEPV 159 >1EB7-A nrPDB Length = 323 Score = 29.6 bits (65), Expect = 0.91, Method: Compositional matrix adjust. Identities = 15/45 (33%), Positives = 20/45 (44%), Gaps = 3/45 (6%) Query: 103 LDANTLGKVASGICDNLLTCVMRAWD---RSKPLLFCPAMNTAMW 144 L NT V +G DN+ T V W R+ P +F N A + Sbjct: 49 LSCNTCHNVGTGGADNVPTSVGHGWQKGPRNSPTVFNAVFNAAQF 93 >4ZAN-A nrPDB Length = 209 Score = 27.7 bits (60), Expect = 3.4, Method: Compositional matrix adjust. Identities = 15/46 (32%), Positives = 24/46 (52%), Gaps = 7/46 (15%) Query: 96 DLLLVAPLDANTLGKVASGICDNLLTCVMRAWD----RSKPLLFCP 137 + +++ P TL VA+G C+NL+ RA D +PL+ P Sbjct: 96 NAMVICPCSTGTLSAVATGACNNLIE---RAADVALKERRPLVLVP 138 >3NZQ-A nrPDB Length = 666 Score = 27.7 bits (60), Expect = 4.0, Method: Compositional matrix adjust. Identities = 27/112 (24%), Positives = 40/112 (35%), Gaps = 16/112 (14%) Query: 25 TGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFYSPQDIPVTLYSDADEWEIWKSRSD 84 +G +LVS ++ + E V T +D P L S WE W+ + Sbjct: 361 SGRAVTAHHTVLVSNIIGVERNEYTVPTA-------PAEDAPRALQS---MWETWQEMHE 410 Query: 85 PVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLLTCVMRAWDRSKPLLFC 136 P LR W L + +D L + G + + RAW L C Sbjct: 411 PGTRRSLREW---LHDSQMD---LHDIHIGYSSGIFSLQERAWAEQLYLSMC 456 >5G2V-A nrPDB Length = 534 Score = 27.3 bits (59), Expect = 5.1, Method: Compositional matrix adjust. Identities = 8/19 (42%), Positives = 14/19 (73%) Query: 67 VTLYSDADEWEIWKSRSDP 85 + Y+D DEWE++ ++DP Sbjct: 463 IHFYNDIDEWEMYDMKADP 481 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1QZU-A nrPDB 359 e-100 1MVN-A nrPDB 295 9e-81 1P3Y-1 nrPDB 127 2e-30 5H75-A nrPDB 127 2e-30 Sequences not found previously or not previously below threshold: 4ZAN-A nrPDB 30 0.69 3FLC-O nrPDB 28 2.0 5G2V-A nrPDB 28 2.7 6GAW-Bm nrPDB 27 4.1 1A0D-A nrPDB 27 6.7 3FR8-A nrPDB 27 8.9 6NCL-a0 nrPDB 26 9.6 CONVERGED! >1QZU-A nrPDB Length = 206 Score = 359 bits (922), Expect = e-100, Method: Composition-based stats. Identities = 203/204 (99%), Positives = 204/204 (100%) Query: 1 MEPKASCPAAAPLMERKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFY 60 MEPKASCPAAAPLMERKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFY Sbjct: 3 MEPKASCPAAAPLMERKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFY 62 Query: 61 SPQDIPVTLYSDADEWEIWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLL 120 SPQDIPVTLYSDADEWE+WKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLL Sbjct: 63 SPQDIPVTLYSDADEWEMWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLL 122 Query: 121 TCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGA 180 TCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGA Sbjct: 123 TCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGA 182 Query: 181 MAEVGTIVDKVKEVLFQHSGFQQS 204 MAEVGTIVDKVKEVLFQHSGFQQS Sbjct: 183 MAEVGTIVDKVKEVLFQHSGFQQS 206 >1MVN-A nrPDB Length = 209 Score = 295 bits (755), Expect = 9e-81, Method: Composition-based stats. Identities = 92/191 (48%), Positives = 119/191 (62%), Gaps = 5/191 (2%) Query: 16 RKFHVLVGVTGSVAALKLPLLVSKLLDIPGLEVAVVTTERAKHFYSPQDIP--VTLYSDA 73 RK VL+ +GSVAA+K L + EV V T+ + HF +P VTLY+D Sbjct: 18 RKPRVLLAASGSVAAIKFGNLCHCFTEWA--EVRAVVTKSSLHFLDKLSLPQEVTLYTDE 75 Query: 74 DEWEIWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLLTCVMRAWDRSKPL 133 DEW W DPVLHI+LRRWAD+L++APL ANTLGK+A G+CDNLLTC++RAWD +KPL Sbjct: 76 DEWSSWNKIGDPVLHIELRRWADVLVIAPLSANTLGKIAGGLCDNLLTCIIRAWDYTKPL 135 Query: 134 LFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLVCGDEGLGAMAEVGTIVDKVKE 193 PAMNT MW +P T + + L G IP + K+L GD G GAMAE I V+ Sbjct: 136 FVAPAMNTLMWNNPFTERHLLSLDELGITLIPPIKKRLASGDYGNGAMAEPSLIYSTVR- 194 Query: 194 VLFQHSGFQQS 204 + ++ QQ+ Sbjct: 195 LFWESQAHQQT 205 >1P3Y-1 nrPDB Length = 194 Score = 127 bits (320), Expect = 2e-30, Method: Composition-based stats. Identities = 33/121 (27%), Positives = 59/121 (48%), Gaps = 5/121 (4%) Query: 47 EVAVVTTERAKHFYSPQDIPVTLYSDADEWEIWKSRSDPVLHIDLRRWADLLLVAPLDAN 106 E+ VV T+ A+ V+ + D + H+++ RWAD+ + P AN Sbjct: 37 EIRVVMTKTAEDLIPAHT--VSYFCDH-VYSEHGENGKRHSHVEIGRWADIYCIIPATAN 93 Query: 107 TLGKVASGICDNLLTCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPC 166 LG+ A+G+ NL+ + A + +F P MN MW + ++ ++QL+ G++ I Sbjct: 94 ILGQTANGVAMNLVATTVLAHPHNT--IFFPNMNDLMWNKTVVSRNIEQLRKDGHIVIEP 151 Query: 167 V 167 V Sbjct: 152 V 152 >5H75-A nrPDB Length = 238 Score = 127 bits (320), Expect = 2e-30, Method: Composition-based stats. Identities = 33/121 (27%), Positives = 59/121 (48%), Gaps = 5/121 (4%) Query: 47 EVAVVTTERAKHFYSPQDIPVTLYSDADEWEIWKSRSDPVLHIDLRRWADLLLVAPLDAN 106 E+ VV T+ A+ V+ + D + H+++ RWAD+ + P AN Sbjct: 44 EIRVVMTKTAEDLIPAHT--VSYFCDH-VYSEHGENGKRHSHVEIGRWADIYCIIPATAN 100 Query: 107 TLGKVASGICDNLLTCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPC 166 LG+ A+G+ NL+ + A + +F P MN MW + ++ ++QL+ G++ I Sbjct: 101 ILGQTANGVAMNLVATTVLAHPHNT--IFFPNMNDLMWNKTVVSRNIEQLRKDGHIVIEP 158 Query: 167 V 167 V Sbjct: 159 V 159 >4ZAN-A nrPDB Length = 209 Score = 30.0 bits (66), Expect = 0.69, Method: Composition-based stats. Identities = 19/74 (25%), Positives = 34/74 (45%), Gaps = 10/74 (13%) Query: 96 DLLLVAPLDANTLGKVASGICDNLLTCVM-RAWDRSKPLLFCPAMNTAMWEHPITAQQVD 154 + +++ P TL VA+G C+NL+ A +PL+ P E P ++ ++ Sbjct: 96 NAMVICPCSTGTLSAVATGACNNLIERAADVALKERRPLVLVPR------EAPFSSIHLE 149 Query: 155 ---QLKAFGYVEIP 165 +L G V +P Sbjct: 150 NMLKLSNLGAVILP 163 >3FLC-O nrPDB Length = 518 Score = 28.5 bits (62), Expect = 2.0, Method: Composition-based stats. Identities = 21/103 (20%), Positives = 40/103 (38%), Gaps = 15/103 (14%) Query: 62 PQDIPVTLYSDADEWEIWKSRSDPVLHIDLRRWADLLLVAPLDANTLGKVASGICDNLLT 121 PQ P + + + + EIW S ++ A +++ + +GI Sbjct: 49 PQYFPKSGWVEHNANEIWNSVQS------------VIAGAFIESGIRPEAIAGIGITNQR 96 Query: 122 CVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEI 164 WD++ P N +W+ ++ DQLK G+ E+ Sbjct: 97 ETTVVWDKTTGQ---PIANAIVWQSRQSSPIADQLKVDGHTEM 136 >5G2V-A nrPDB Length = 534 Score = 28.1 bits (61), Expect = 2.7, Method: Composition-based stats. Identities = 8/19 (42%), Positives = 14/19 (73%) Query: 67 VTLYSDADEWEIWKSRSDP 85 + Y+D DEWE++ ++DP Sbjct: 463 IHFYNDIDEWEMYDMKADP 481 >6GAW-Bm nrPDB Length = 159 Score = 27.3 bits (59), Expect = 4.1, Method: Composition-based stats. Identities = 19/83 (22%), Positives = 33/83 (39%), Gaps = 10/83 (12%) Query: 113 SGICDNLLTCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAKKLV 172 SG+ LTC + R + + P + P+ A+ V+++K + P + + Sbjct: 7 SGLARRSLTCAVSGAPRRE--FWSPFRKE---KQPVVAETVEEVKKEPILVCPPIQSRTY 61 Query: 173 CGDEGLGAMAEVGTIVDKVKEVL 195 E L + E VKEV Sbjct: 62 IPPEDLQSRLE-----SHVKEVF 79 >1A0D-A nrPDB Length = 440 Score = 26.9 bits (58), Expect = 6.7, Method: Composition-based stats. Identities = 9/41 (21%), Positives = 17/41 (41%) Query: 34 PLLVSKLLDIPGLEVAVVTTERAKHFYSPQDIPVTLYSDAD 74 ++ G+++A E A F+ +IP + D D Sbjct: 61 GNMIRPWNKYSGMDLAKARVEAAFEFFEKLNIPFFCFHDVD 101 >3FR8-A nrPDB Length = 525 Score = 26.6 bits (57), Expect = 8.9, Method: Composition-based stats. Identities = 11/47 (23%), Positives = 20/47 (42%), Gaps = 2/47 (4%) Query: 35 LLVSKLLDIP-GLEVAVVTTERAKHFYSPQDIPVTLYSDADEWEIWK 80 +L D+ G E+ V + FY + +P + D+ +WK Sbjct: 327 ILYECYEDVASGSEIRSVVL-AGRRFYEKEGLPAFPMGNIDQTRMWK 372 >6NCL-a0 nrPDB Length = 352 Score = 26.2 bits (56), Expect = 9.6, Method: Composition-based stats. Identities = 18/93 (19%), Positives = 34/93 (36%), Gaps = 7/93 (7%) Query: 110 KVASGICDNLLTCVMRAWDRSKPLLFCPAMNTAMWEHPITAQQVDQLKAFGYVEIPCVAK 169 K+ I DN + R ++ + + + AQQ +QL E Sbjct: 18 KMTDAISDNQIIATSRFFN------IARDVADVVVSNTKLAQQYEQLSIDSLKEYLVSVA 71 Query: 170 KLVCGDEGLGAMAEVGTIVDKVKEVLFQHSGFQ 202 K V D A+V ++ K++ + + +Q Sbjct: 72 KFVAVDYSNTTSADVDDLIHKLR-LFIEEECYQ 103 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.134 0.408 Lambda K H 0.267 0.0415 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,345,660 Number of Sequences: 36641 Number of extensions: 321931 Number of successful extensions: 820 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 797 Number of HSP's gapped (non-prelim): 25 length of query: 204 length of database: 10,336,785 effective HSP length: 92 effective length of query: 112 effective length of database: 6,965,813 effective search space: 780171056 effective search space used: 780171056 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 57 (26.5 bits)